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The map label for this gene is bkdB [H]

Identifier: 150398026

GI number: 150398026

Start: 2952752

End: 2954032

Strand: Direct

Name: bkdB [H]

Synonym: Smed_2828

Alternate gene names: 150398026

Gene position: 2952752-2954032 (Clockwise)

Preceding gene: 150398025

Following gene: 150398027

Centisome position: 78.08

GC content: 66.67

Gene sequence:

>1281_bases
ATGGGCGAATTCACCATCAAGATGCCGGATGTCGGCGAAGGCGTCGCGGAGGCCGAACTCGTCGAATGGCATGTGAAGCC
GGGAGACCCGGTACGCGAAGACATGGTGCTCGCCGCCGTCATGACCGACAAGGCCACGGTCGAAATCCCCTCCCCCGTCT
CGGGCAAGGTGCTCTGGCTCGGCGCTGAAGTCGGCGACACGATCGCGGTGAAGGCGCCGCTGGTCAGGATCGAGACCTCA
GCTGAGGCGGCAGAGGCTGCTCCGGACAGTGTTCCGGAGGCGCTGGCGGAAAAAGTGCTCGACCAGCCCGTCGCCGTCTC
TTCCCGATCCGAGGCCGGGGCAGCTGCGCAGGCTAAAAGGCCGGCGCCGGAACCTGCTCCGGCGCCCCGCGAAACCCCAA
GCTTTTCGGCAAAGCCCCTCGCCTCCCCGGCCGTGCGGCTGCGCGCCAGAGAGAGCGGTATCGACCTCAGGCAGGTAACC
GGCACAGGGCCGGCAGGCCGGATCACCCATGAGGATCTCGACCTCTTCCTCAGCCGGGGCGCCGGCCCCGTGGCCGCTCC
GGCCGGACTGGTCCGCAAGACCGCGGTCGAGGAGGTCAAGATGACCGGCCTCAGGCGGCGGATCGCCGAGAAAATGTCGC
TCTCCGCGTCGCGCATCCCCCACATCACCTATGTGGAAGAGGTCGACATGACCGCGCTCGAGGATTTGCGCGCGACGATG
AACCGCGAGCGCAAGTCCGAACAGGCCAAGCTGACGATCCTGCCATTCCTGATGCGGGCGCTCGTCAGAACCGCCGCCGA
ACTGCCAGGCGTCAATGCCACATTCGACGATCATGCCGGCATCATCCATCGTCACTCCGCCGTCCATATCGGCGTCGCCA
CCCAGACACCCGCCGGCCTGATGGTTCCGGTGGTCCGCCATGCCGAGGCGCGCGGGATCTGGGACTGCGCGACCGAGCTC
AGCCGCCTGGCGGAGGCCGCCCGCACCGGAACCGCGACGCGCGACGAGCTCACCGGTTCGACCATCACCATCTCCTCGCT
CGGCGCCATTGGCGGGATCGCCTCGACCCCGGTCATCAACCATCCGGAAGTGGCGATCGTCGGCGTCAACAAGATCGCCG
TCCGCCCCGTCTGGGACGGCGCGCAATTCGTGCCTCGCAAGATCATGAATCTCTCGTCGAGCTTCGACCACCGGGTGATC
GACGGCTGGGATGCCGCGGTTTTCGTTCAGCGGCTGAAGACGCTGATCGAAACGCCGGCGCTGATTTTCGTTGAAGGATA
G

Upstream 100 bases:

>100_bases
GGGCTGGGATACCCCCTATCCGCACGCCCAGGAATGGGACTATTTCCCAGGCCCGGCACGCGTCGGGCGCGCGCTCGCCG
AAGCGATGGAGGGCTGACGC

Downstream 100 bases:

>100_bases
GCGTTATGAAGGAAATCTCCTGCAAACTCCTGGTTCTTGGCGCCGGTCCCGGCGGCTATGTCACGGCGATCCGCGCCGCC
CAGCTCGGCGTCAACACCGT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MGEFTIKMPDVGEGVAEAELVEWHVKPGDPVREDMVLAAVMTDKATVEIPSPVSGKVLWLGAEVGDTIAVKAPLVRIETS
AEAAEAAPDSVPEALAEKVLDQPVAVSSRSEAGAAAQAKRPAPEPAPAPRETPSFSAKPLASPAVRLRARESGIDLRQVT
GTGPAGRITHEDLDLFLSRGAGPVAAPAGLVRKTAVEEVKMTGLRRRIAEKMSLSASRIPHITYVEEVDMTALEDLRATM
NRERKSEQAKLTILPFLMRALVRTAAELPGVNATFDDHAGIIHRHSAVHIGVATQTPAGLMVPVVRHAEARGIWDCATEL
SRLAEAARTGTATRDELTGSTITISSLGAIGGIASTPVINHPEVAIVGVNKIAVRPVWDGAQFVPRKIMNLSSSFDHRVI
DGWDAAVFVQRLKTLIETPALIFVEG

Sequences:

>Translated_426_residues
MGEFTIKMPDVGEGVAEAELVEWHVKPGDPVREDMVLAAVMTDKATVEIPSPVSGKVLWLGAEVGDTIAVKAPLVRIETS
AEAAEAAPDSVPEALAEKVLDQPVAVSSRSEAGAAAQAKRPAPEPAPAPRETPSFSAKPLASPAVRLRARESGIDLRQVT
GTGPAGRITHEDLDLFLSRGAGPVAAPAGLVRKTAVEEVKMTGLRRRIAEKMSLSASRIPHITYVEEVDMTALEDLRATM
NRERKSEQAKLTILPFLMRALVRTAAELPGVNATFDDHAGIIHRHSAVHIGVATQTPAGLMVPVVRHAEARGIWDCATEL
SRLAEAARTGTATRDELTGSTITISSLGAIGGIASTPVINHPEVAIVGVNKIAVRPVWDGAQFVPRKIMNLSSSFDHRVI
DGWDAAVFVQRLKTLIETPALIFVEG
>Mature_425_residues
GEFTIKMPDVGEGVAEAELVEWHVKPGDPVREDMVLAAVMTDKATVEIPSPVSGKVLWLGAEVGDTIAVKAPLVRIETSA
EAAEAAPDSVPEALAEKVLDQPVAVSSRSEAGAAAQAKRPAPEPAPAPRETPSFSAKPLASPAVRLRARESGIDLRQVTG
TGPAGRITHEDLDLFLSRGAGPVAAPAGLVRKTAVEEVKMTGLRRRIAEKMSLSASRIPHITYVEEVDMTALEDLRATMN
RERKSEQAKLTILPFLMRALVRTAAELPGVNATFDDHAGIIHRHSAVHIGVATQTPAGLMVPVVRHAEARGIWDCATELS
RLAEAARTGTATRDELTGSTITISSLGAIGGIASTPVINHPEVAIVGVNKIAVRPVWDGAQFVPRKIMNLSSSFDHRVID
GWDAAVFVQRLKTLIETPALIFVEG

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=446, Percent_Identity=32.5112107623318, Blast_Score=206, Evalue=4e-53,
Organism=Homo sapiens, GI31711992, Length=417, Percent_Identity=28.0575539568345, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=33.0434782608696, Blast_Score=138, Evalue=8e-33,
Organism=Homo sapiens, GI203098753, Length=457, Percent_Identity=24.507658643326, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI203098816, Length=457, Percent_Identity=24.507658643326, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=30.625, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1786305, Length=422, Percent_Identity=31.9905213270142, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786946, Length=435, Percent_Identity=29.8850574712644, Blast_Score=183, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=32.2654462242563, Blast_Score=199, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=29.6037296037296, Blast_Score=165, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17560088, Length=421, Percent_Identity=29.4536817102138, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=311, Percent_Identity=24.4372990353698, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=31.7307692307692, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6324258, Length=448, Percent_Identity=27.6785714285714, Blast_Score=148, Evalue=2e-36,
Organism=Drosophila melanogaster, GI18859875, Length=440, Percent_Identity=30.4545454545455, Blast_Score=171, Evalue=6e-43,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=33.4801762114537, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=26.8722466960352, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=26.8722466960352, Blast_Score=105, Evalue=9e-23,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 45260; Mature: 45129

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEFTIKMPDVGEGVAEAELVEWHVKPGDPVREDMVLAAVMTDKATVEIPSPVSGKVLWL
CCCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHEEEEEECCCCEEECCCCCCCEEEEE
GAEVGDTIAVKAPLVRIETSAEAAEAAPDSVPEALAEKVLDQPVAVSSRSEAGAAAQAKR
ECCCCCEEEEECCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
PAPEPAPAPRETPSFSAKPLASPAVRLRARESGIDLRQVTGTGPAGRITHEDLDLFLSRG
CCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCC
AGPVAAPAGLVRKTAVEEVKMTGLRRRIAEKMSLSASRIPHITYVEEVDMTALEDLRATM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
NRERKSEQAKLTILPFLMRALVRTAAELPGVNATFDDHAGIIHRHSAVHIGVATQTPAGL
HHHHHHHHCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCH
MVPVVRHAEARGIWDCATELSRLAEAARTGTATRDELTGSTITISSLGAIGGIASTPVIN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHCCCCCCCCCC
HPEVAIVGVNKIAVRPVWDGAQFVPRKIMNLSSSFDHRVIDGWDAAVFVQRLKTLIETPA
CCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCC
LIFVEG
EEEEEC
>Mature Secondary Structure 
GEFTIKMPDVGEGVAEAELVEWHVKPGDPVREDMVLAAVMTDKATVEIPSPVSGKVLWL
CCEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHEEEEEECCCCEEECCCCCCCEEEEE
GAEVGDTIAVKAPLVRIETSAEAAEAAPDSVPEALAEKVLDQPVAVSSRSEAGAAAQAKR
ECCCCCEEEEECCEEEEECCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCC
PAPEPAPAPRETPSFSAKPLASPAVRLRARESGIDLRQVTGTGPAGRITHEDLDLFLSRG
CCCCCCCCCCCCCCCCCCCCCCHHHHEEHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCC
AGPVAAPAGLVRKTAVEEVKMTGLRRRIAEKMSLSASRIPHITYVEEVDMTALEDLRATM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHH
NRERKSEQAKLTILPFLMRALVRTAAELPGVNATFDDHAGIIHRHSAVHIGVATQTPAGL
HHHHHHHHCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCH
MVPVVRHAEARGIWDCATELSRLAEAARTGTATRDELTGSTITISSLGAIGGIASTPVIN
HHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEEEHHHHHCCCCCCCCCC
HPEVAIVGVNKIAVRPVWDGAQFVPRKIMNLSSSFDHRVIDGWDAAVFVQRLKTLIETPA
CCCEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCCCCEECCCHHHHHHHHHHHHHCCCC
LIFVEG
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3046941 [H]