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The map label for this gene is cobJ [H]

Identifier: 150398013

GI number: 150398013

Start: 2940176

End: 2940940

Strand: Reverse

Name: cobJ [H]

Synonym: Smed_2815

Alternate gene names: 150398013

Gene position: 2940940-2940176 (Counterclockwise)

Preceding gene: 150398014

Following gene: 150398011

Centisome position: 77.76

GC content: 66.67

Gene sequence:

>765_bases
ATGAGGGGAACGCTCTTCATCGTCGGCACCGGGCCTGGCAATCCGGCACAGATGACGCCCGAGGCCGAGAATGCGATCGC
TGCAGCGACCGAATTCTTCGGCTATGGCCCCTATCTCGATCGCCTGCAACTTCGCGCGGATCAACGGCGCATAGCATCGG
ACAACCGCGAGGAACTGGACCGCGCTCAGGCGGCACTCGCGCGCGCCGCGGCAGGGGTGAATGTCTGCGTCGTCTCCGGC
GGCGACCCCGGCATCTTCGCCATGGCCGCCGCCGTCTGCGAGGCGATCGACAACGGACCGCAGGAATGGCGCGAAGTCGA
TCTCACGATCACGCCCGGCGTCACGGCAATGCTCGCGGTCGCCGCACGGATCGGGGCGCCGCTGGGGCACGATTTCTGTG
TCATGTCGCTTTCCGACAATCTGAAGCCCTGGGAGGTCATCACCCGCCGGCTCCAGCTTGCGGCCGAAGCGGGGCTGGTC
ATCGCGCTTTACAATCCGATCAGCAAGGCTCGCCCCTGGCAACTCGGCGAGGCTTTCGAGGTTCTGCGGCAGGTGCTGCC
GGCAACCGTGCCGGTGATCTTCGGCCACGCAGCCGGGCGGCCCGACGAGCGCATCGAAGTGATGCCGCTCGGCGAGGCCG
AGCCGGGTCGCGCTGACATGGCGACCTGCGTGATCATCGGCTCGCCGGAGACACGTACCATCCTGCGCGACGGGAAGCAT
GATCTCGTCTACACGCCGCGGTCCTTTACCGGAGCAAAGCGTTGA

Upstream 100 bases:

>100_bases
AGAAGCCGGACGACGAGGCGCCCTATTTCTCGCTGGTGCTGGTTCCGGGTTGGAAGGACAGGCCCGGCGGGAAGGACAGA
CCGGGATTGGAGGGCAAGGT

Downstream 100 bases:

>100_bases
CGGATGCGTTCGAGCGCCTCGCTACAGTCGCCGACCGACGGAACGTCGGCAGGCTTTCGGCGCTCGACCATCACCACCTC
GATCCCGAGCTTTCGCGCCG

Product: precorrin-3B C17-methyltransferase

Products: NA

Alternate protein names: Precorrin-3 methylase; Precorrin-3 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MRGTLFIVGTGPGNPAQMTPEAENAIAAATEFFGYGPYLDRLQLRADQRRIASDNREELDRAQAALARAAAGVNVCVVSG
GDPGIFAMAAAVCEAIDNGPQEWREVDLTITPGVTAMLAVAARIGAPLGHDFCVMSLSDNLKPWEVITRRLQLAAEAGLV
IALYNPISKARPWQLGEAFEVLRQVLPATVPVIFGHAAGRPDERIEVMPLGEAEPGRADMATCVIIGSPETRTILRDGKH
DLVYTPRSFTGAKR

Sequences:

>Translated_254_residues
MRGTLFIVGTGPGNPAQMTPEAENAIAAATEFFGYGPYLDRLQLRADQRRIASDNREELDRAQAALARAAAGVNVCVVSG
GDPGIFAMAAAVCEAIDNGPQEWREVDLTITPGVTAMLAVAARIGAPLGHDFCVMSLSDNLKPWEVITRRLQLAAEAGLV
IALYNPISKARPWQLGEAFEVLRQVLPATVPVIFGHAAGRPDERIEVMPLGEAEPGRADMATCVIIGSPETRTILRDGKH
DLVYTPRSFTGAKR
>Mature_254_residues
MRGTLFIVGTGPGNPAQMTPEAENAIAAATEFFGYGPYLDRLQLRADQRRIASDNREELDRAQAALARAAAGVNVCVVSG
GDPGIFAMAAAVCEAIDNGPQEWREVDLTITPGVTAMLAVAARIGAPLGHDFCVMSLSDNLKPWEVITRRLQLAAEAGLV
IALYNPISKARPWQLGEAFEVLRQVLPATVPVIFGHAAGRPDERIEVMPLGEAEPGRADMATCVIIGSPETRTILRDGKH
DLVYTPRSFTGAKR

Specific function: Methyltransferase that catalyzes the methylation of C-17 in precorrin-3B to form precorrin-4 [H]

COG id: COG1010

COG function: function code H; Precorrin-3B methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006363 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.131 [H]

Molecular weight: Translated: 27200; Mature: 27200

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTLFIVGTGPGNPAQMTPEAENAIAAATEFFGYGPYLDRLQLRADQRRIASDNREELD
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
RAQAALARAAAGVNVCVVSGGDPGIFAMAAAVCEAIDNGPQEWREVDLTITPGVTAMLAV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEEECCCHHHHHHH
AARIGAPLGHDFCVMSLSDNLKPWEVITRRLQLAAEAGLVIALYNPISKARPWQLGEAFE
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
VLRQVLPATVPVIFGHAAGRPDERIEVMPLGEAEPGRADMATCVIIGSPETRTILRDGKH
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHCCCC
DLVYTPRSFTGAKR
CEEECCCCCCCCCC
>Mature Secondary Structure
MRGTLFIVGTGPGNPAQMTPEAENAIAAATEFFGYGPYLDRLQLRADQRRIASDNREELD
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHH
RAQAALARAAAGVNVCVVSGGDPGIFAMAAAVCEAIDNGPQEWREVDLTITPGVTAMLAV
HHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHEEEEEECCCHHHHHHH
AARIGAPLGHDFCVMSLSDNLKPWEVITRRLQLAAEAGLVIALYNPISKARPWQLGEAFE
HHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
VLRQVLPATVPVIFGHAAGRPDERIEVMPLGEAEPGRADMATCVIIGSPETRTILRDGKH
HHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCHHHHHCCCC
DLVYTPRSFTGAKR
CEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2211521; 8226690 [H]