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The map label for this gene is lig [H]

Identifier: 150398002

GI number: 150398002

Start: 2926730

End: 2928343

Strand: Direct

Name: lig [H]

Synonym: Smed_2804

Alternate gene names: 150398002

Gene position: 2926730-2928343 (Clockwise)

Preceding gene: 150398001

Following gene: 150398003

Centisome position: 77.39

GC content: 63.32

Gene sequence:

>1614_bases
ATGAAAGCCTTCGCCGAATTGCTCGACCGGCTGGTGCTGACGCCGCAGCGAAACGCCAAGATCCGGCTGCTCGTCGATTA
CTTCCACAGCGCGCCCGATCCGAGCCGCGGCTACGCGTTGGCCGCGATCGCCGGAACGCTCTCGCTGAACACGGTGAAGC
CTGCTCTCATTCGCGACCTGCTGCTCGAGCGTATGGACGGCGTACTGTTCCAGTACTCCTACGATTATGTAGGCGATCTC
GCGGAAACAGTCTCTCTGGCCTGGGAGCCGCCGGCGGAGGTCGCCCCCGAGGACATTCCGCTCGGGGAAGTCGTCGAACG
GCTTCAAAAAGCGGGCCGTTCCGAGGTCCGTTCGCTCGTCCGCGAGATGCTGGACCGGCTCGATACCTCCGGCCGCTTCG
CCCTCCTGAAGCTCGCGACGGGAGGCCTGCGTATCGGGGTCTCGGCACGGCTTGCGAAACAGGCGCTTGCGGAGATGGGC
AGCAGGGAGGTGACAGAGATCGAAACGCTCTGGCACGGCCTGGAGCCGCCCTACCGGCCGCTTTTCCTCTGGCTCACCGG
CGAGGGCGAAATGCCGTTGCTCAAGACGCCGGCGATATTTCACTCGGTGATGCTCGCGACAGCGGTGGGCGAAGGCGAGC
TCGATGGTCTCGATCCGAGCGACTACGCGGCCGAGTGGAAATGGGACGGTATCCGCGTCCAACTCGCCAATGTCGGCGGG
GTCCGCCGTCTCTACTCTCGCAGCGGTGACGAGATTTCCTCCGCCTTTCCCGAAATCATCGAGGTTGCCGGGATTGACGG
CGTCATCGACGGCGAGCTTCTGGTCGGCGGAACGATGCGCAGCAACCGCGCCACCGGTACGTTCGCCGACCTGCAGCAGA
GGCTGAACCGGAAGACCGTCAGTCGCAAGCTCCTCGATGATTATCCGGCATTCATTCGCGCCTATGACGTGCTCTTTTCA
GGCGAGCGCGATATCAGGCCCGAGCCCTTCCATGTCCGCCGCGGGGCCCTGTCGGCGCTGATCGAAGCAGCCTCGCCGCA
GCATTTCGATCTCTCGCCACTCGTCCCGTTTTCGAACTGGAAGGAGCTCGACGAGCTACGAGCGAAGCCGCCCGACCCCG
TGATCGAGGGCGTCATGCTGAAACGTCTGGACTCGCCCTATCTGGCCGGAAGATCCAAGGGCCCCTGGTTCAAGTGGAAG
CGTGCGCCCTTCAATATAGATGCCGTGCTGATGTATGCGCAACGCGGCCACGGCAAACGTTCAAGCTATTATTCCGACTT
CACCTTCGGAGTTTGGACGGAGGGGGAAGACGGAACGAGCCTCGTTCCGGTCGGCAAGGCCTATTTCGGTTTCACCGATG
CCGAGCTCGAGATTCTCGACCGTTTCGTGCGCGACAATACGGTCGAACGTTTCGGTCCCGTGCGCGCGGTGCGCGCCGAA
CCCGACAAGGGTTTCGTCGTCGAGGTCGCCTTCGAAGGCCTCAACCGCTCCACCCGTCACAAGTCCGGCGTAGCCATGCG
CTTTCCGCGGATTGCCCGGCTCAGACCCGACAAGCTGCCACGCGACGCGGACCGACTGGAAACGTTGCAGGCGATGATCG
GGCCGCCAACTTGA

Upstream 100 bases:

>100_bases
ACATGGACGCGAGGAAGCGCTGGTTCGCTGGTGCCAGTTGCAGGGAATCCCGGCACGCCCGCTGCACCTTGTCGGCTACG
ATGACGAGGGGGAGTGAGCG

Downstream 100 bases:

>100_bases
AACGCCCGTCACGGCGTGTAACGAGAGCTATTTCAGTAAATTCGCATGGAGGTTTGTTAAGGAGAGTCTGGAATTATCCG
GAAGCTGCTGCATGCCGTGC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKAFAELLDRLVLTPQRNAKIRLLVDYFHSAPDPSRGYALAAIAGTLSLNTVKPALIRDLLLERMDGVLFQYSYDYVGDL
AETVSLAWEPPAEVAPEDIPLGEVVERLQKAGRSEVRSLVREMLDRLDTSGRFALLKLATGGLRIGVSARLAKQALAEMG
SREVTEIETLWHGLEPPYRPLFLWLTGEGEMPLLKTPAIFHSVMLATAVGEGELDGLDPSDYAAEWKWDGIRVQLANVGG
VRRLYSRSGDEISSAFPEIIEVAGIDGVIDGELLVGGTMRSNRATGTFADLQQRLNRKTVSRKLLDDYPAFIRAYDVLFS
GERDIRPEPFHVRRGALSALIEAASPQHFDLSPLVPFSNWKELDELRAKPPDPVIEGVMLKRLDSPYLAGRSKGPWFKWK
RAPFNIDAVLMYAQRGHGKRSSYYSDFTFGVWTEGEDGTSLVPVGKAYFGFTDAELEILDRFVRDNTVERFGPVRAVRAE
PDKGFVVEVAFEGLNRSTRHKSGVAMRFPRIARLRPDKLPRDADRLETLQAMIGPPT

Sequences:

>Translated_537_residues
MKAFAELLDRLVLTPQRNAKIRLLVDYFHSAPDPSRGYALAAIAGTLSLNTVKPALIRDLLLERMDGVLFQYSYDYVGDL
AETVSLAWEPPAEVAPEDIPLGEVVERLQKAGRSEVRSLVREMLDRLDTSGRFALLKLATGGLRIGVSARLAKQALAEMG
SREVTEIETLWHGLEPPYRPLFLWLTGEGEMPLLKTPAIFHSVMLATAVGEGELDGLDPSDYAAEWKWDGIRVQLANVGG
VRRLYSRSGDEISSAFPEIIEVAGIDGVIDGELLVGGTMRSNRATGTFADLQQRLNRKTVSRKLLDDYPAFIRAYDVLFS
GERDIRPEPFHVRRGALSALIEAASPQHFDLSPLVPFSNWKELDELRAKPPDPVIEGVMLKRLDSPYLAGRSKGPWFKWK
RAPFNIDAVLMYAQRGHGKRSSYYSDFTFGVWTEGEDGTSLVPVGKAYFGFTDAELEILDRFVRDNTVERFGPVRAVRAE
PDKGFVVEVAFEGLNRSTRHKSGVAMRFPRIARLRPDKLPRDADRLETLQAMIGPPT
>Mature_537_residues
MKAFAELLDRLVLTPQRNAKIRLLVDYFHSAPDPSRGYALAAIAGTLSLNTVKPALIRDLLLERMDGVLFQYSYDYVGDL
AETVSLAWEPPAEVAPEDIPLGEVVERLQKAGRSEVRSLVREMLDRLDTSGRFALLKLATGGLRIGVSARLAKQALAEMG
SREVTEIETLWHGLEPPYRPLFLWLTGEGEMPLLKTPAIFHSVMLATAVGEGELDGLDPSDYAAEWKWDGIRVQLANVGG
VRRLYSRSGDEISSAFPEIIEVAGIDGVIDGELLVGGTMRSNRATGTFADLQQRLNRKTVSRKLLDDYPAFIRAYDVLFS
GERDIRPEPFHVRRGALSALIEAASPQHFDLSPLVPFSNWKELDELRAKPPDPVIEGVMLKRLDSPYLAGRSKGPWFKWK
RAPFNIDAVLMYAQRGHGKRSSYYSDFTFGVWTEGEDGTSLVPVGKAYFGFTDAELEILDRFVRDNTVERFGPVRAVRAE
PDKGFVVEVAFEGLNRSTRHKSGVAMRFPRIARLRPDKLPRDADRLETLQAMIGPPT

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71985269, Length=338, Percent_Identity=26.3313609467456, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320038, Length=515, Percent_Identity=24.2718446601942, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 59882; Mature: 59882

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFAELLDRLVLTPQRNAKIRLLVDYFHSAPDPSRGYALAAIAGTLSLNTVKPALIRDL
CCHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHH
LLERMDGVLFQYSYDYVGDLAETVSLAWEPPAEVAPEDIPLGEVVERLQKAGRSEVRSLV
HHHHHCCEEEEECHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
REMLDRLDTSGRFALLKLATGGLRIGVSARLAKQALAEMGSREVTEIETLWHGLEPPYRP
HHHHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCE
LFLWLTGEGEMPLLKTPAIFHSVMLATAVGEGELDGLDPSDYAAEWKWDGIRVQLANVGG
EEEEEECCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEEEECCCH
VRRLYSRSGDEISSAFPEIIEVAGIDGVIDGELLVGGTMRSNRATGTFADLQQRLNRKTV
HHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
SRKLLDDYPAFIRAYDVLFSGERDIRPEPFHVRRGALSALIEAASPQHFDLSPLVPFSNW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
KELDELRAKPPDPVIEGVMLKRLDSPYLAGRSKGPWFKWKRAPFNIDAVLMYAQRGHGKR
HHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCHHHEEEEECCCCCCC
SSYYSDFTFGVWTEGEDGTSLVPVGKAYFGFTDAELEILDRFVRDNTVERFGPVRAVRAE
CCCCCCCEEEEEECCCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCHHHHCCCEEEECC
PDKGFVVEVAFEGLNRSTRHKSGVAMRFPRIARLRPDKLPRDADRLETLQAMIGPPT
CCCCEEEEEEECCCCCCHHCCCCCEEECCHHHCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKAFAELLDRLVLTPQRNAKIRLLVDYFHSAPDPSRGYALAAIAGTLSLNTVKPALIRDL
CCHHHHHHHHHHCCCCCCCEEEEEEEHHHCCCCCCCCEEEEEEEHHHCCCCCCHHHHHHH
LLERMDGVLFQYSYDYVGDLAETVSLAWEPPAEVAPEDIPLGEVVERLQKAGRSEVRSLV
HHHHHCCEEEEECHHHHHHHHHHHHCCCCCCHHCCCCCCCHHHHHHHHHHCCHHHHHHHH
REMLDRLDTSGRFALLKLATGGLRIGVSARLAKQALAEMGSREVTEIETLWHGLEPPYRP
HHHHHHCCCCCCEEEEEEECCCEEECHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCE
LFLWLTGEGEMPLLKTPAIFHSVMLATAVGEGELDGLDPSDYAAEWKWDGIRVQLANVGG
EEEEEECCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCEEEEEECCCH
VRRLYSRSGDEISSAFPEIIEVAGIDGVIDGELLVGGTMRSNRATGTFADLQQRLNRKTV
HHHHHHCCCCHHHHHHHHHHHHCCCCCEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHH
SRKLLDDYPAFIRAYDVLFSGERDIRPEPFHVRRGALSALIEAASPQHFDLSPLVPFSNW
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCH
KELDELRAKPPDPVIEGVMLKRLDSPYLAGRSKGPWFKWKRAPFNIDAVLMYAQRGHGKR
HHHHHHHCCCCCHHHHHHHHHHCCCCHHCCCCCCCCCEEECCCCCHHHEEEEECCCCCCC
SSYYSDFTFGVWTEGEDGTSLVPVGKAYFGFTDAELEILDRFVRDNTVERFGPVRAVRAE
CCCCCCCEEEEEECCCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCHHHHCCCEEEECC
PDKGFVVEVAFEGLNRSTRHKSGVAMRFPRIARLRPDKLPRDADRLETLQAMIGPPT
CCCCEEEEEEECCCCCCHHCCCCCEEECCHHHCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA