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The map label for this gene is gltX [H]

Identifier: 150397997

GI number: 150397997

Start: 2920720

End: 2922177

Strand: Direct

Name: gltX [H]

Synonym: Smed_2799

Alternate gene names: 150397997

Gene position: 2920720-2922177 (Clockwise)

Preceding gene: 150397996

Following gene: 150397998

Centisome position: 77.23

GC content: 60.77

Gene sequence:

>1458_bases
ATGGCAGATTCTGCAGTCCGGGTGCGTATCGCACCTTCCCCCACCGGCGAGCCGCATGTCGGCACCGCTTATATCGCGCT
CTTCAACTATCTCTTCGCCAAGAAGCACGGCGGCGAATTTATTCTGCGCATTGAGGACACGGATGCGACGCGCTCGACGC
CCGAATTCGAGAAGAAAGTGCTCGACGCACTGAAATGGTGCGGGCTGGAATGGTCGGAAGGTCCCGATATTGGCGGCCCC
TGCGGCCCCTATCGCCAGAGCGACCGCAAGGACATCTATAAGCCCTATGTCGAACAGATCGTGGCAAACGGCCACGGCTT
CCGCTGCTTCTGCACGCCGGAGCGGTTGGAGCAGATGCGCGAGGCGCAGCGGGCCGCCGGCAAGCCGCCGAAATATGACG
GTCTCTGCCTCAGCCTCTCGGCCGAGGAAGTAACTTCGCGGGTAGCCGCCGGCGAGCCGCATGTCGTGCGTATGAAGATA
CCCACCGAGGGTTCCTGCAAGTTCCGCGACGGCGTCTATGGCGACGTGGAGATACCGTGGGAAGCCGTCGACATGCAGGT
GCTGCTCAAGGCCGACGGCATGCCGACCTATCACATGGCGAACGTCGTCGACGACCATCTGATGAAGATCACGCATGTCG
CACGCGGCGAGGAATGGCTCGCCTCCGTTCCGAAGCACATCCTGATCTATCAGTATCTCGGTCTTGAACCGCCTGTCTTC
ATGCATCTGTCGCTGATGCGCAACGCCGACAAGTCGAAGCTGTCGAAGCGCAAGAACCCGACCTCGATCTCCTATTACAC
CGCTCTCGGTTACCTGCCGGAAGCGCTCATGAACTTCCTCGGCCTGTTCTTCATCCAGATTGCCGAGGGCGAAGAACTGC
TGACCATGGCGGAGCTGGCGGAGAAGTTCGATCCGGAAAACCTTTCGAAGGCCGGCGCGATCTTCGACATTCAGAAGCTC
GACTGGCTGAACGCACGCTGGATCCGCGAAAAGCTCTCGGAAGAGCAATTCGCAGCAAAGGTCCTTTCATGGGCCATGGA
CAACGACCGTCTGAGGGAGGGCCTGAAGCTCTCGCAGACCCGCATCTCCAAGCTTGGCGAACTGCCGGACCTCGCCGCCT
TCCTCTTCAAATCGGACCTCGGCCTGCAGCCCGCCGCCTTTGCCGGGGTGAAGGCCTCGCCCGAGGAAATGCTGGAGATT
CTGAACACCGTTCAGCCGGATCTCGAAAAGATCCTGGAATGGAACAAGGAATCGATCGAGACTGAACTGCGCGCCTGCGC
CGAGCGCATGGGCAAGAAGCTGAAGGCCGTCGTGGCGCCCCTCTTTGTCGCCGTCTCGGGTTCGCAACGCTCGCTGCCGC
TCTTCGATTCGATGGAACTGCTCGGCCGATCTGTCGTGCGTCAGCGGTTGAAGGTGGCCGCACAGGTCGTCGCCTCCATG
GCAGGCAGCGGGAAGTAA

Upstream 100 bases:

>100_bases
TGGCGCCCTTGCGGGCCTTTCCTTCCCATAAAATGCGCGCCCGAAAGCCGGAGGATATTCCGGCAAGGGCCCCGAAAGCA
CGTCAAGACGGGCAAAGACA

Downstream 100 bases:

>100_bases
GGACGAGACCATGACCGATAAGACAGAAAACTCCGGCCTTTCCTCCGATGCGACGGAAGTACGCGCCCAGAAGCTGAAGC
TCCTGCGCGAGCAGATCGGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 485; Mature: 484

Protein sequence:

>485_residues
MADSAVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFEKKVLDALKWCGLEWSEGPDIGGP
CGPYRQSDRKDIYKPYVEQIVANGHGFRCFCTPERLEQMREAQRAAGKPPKYDGLCLSLSAEEVTSRVAAGEPHVVRMKI
PTEGSCKFRDGVYGDVEIPWEAVDMQVLLKADGMPTYHMANVVDDHLMKITHVARGEEWLASVPKHILIYQYLGLEPPVF
MHLSLMRNADKSKLSKRKNPTSISYYTALGYLPEALMNFLGLFFIQIAEGEELLTMAELAEKFDPENLSKAGAIFDIQKL
DWLNARWIREKLSEEQFAAKVLSWAMDNDRLREGLKLSQTRISKLGELPDLAAFLFKSDLGLQPAAFAGVKASPEEMLEI
LNTVQPDLEKILEWNKESIETELRACAERMGKKLKAVVAPLFVAVSGSQRSLPLFDSMELLGRSVVRQRLKVAAQVVASM
AGSGK

Sequences:

>Translated_485_residues
MADSAVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFEKKVLDALKWCGLEWSEGPDIGGP
CGPYRQSDRKDIYKPYVEQIVANGHGFRCFCTPERLEQMREAQRAAGKPPKYDGLCLSLSAEEVTSRVAAGEPHVVRMKI
PTEGSCKFRDGVYGDVEIPWEAVDMQVLLKADGMPTYHMANVVDDHLMKITHVARGEEWLASVPKHILIYQYLGLEPPVF
MHLSLMRNADKSKLSKRKNPTSISYYTALGYLPEALMNFLGLFFIQIAEGEELLTMAELAEKFDPENLSKAGAIFDIQKL
DWLNARWIREKLSEEQFAAKVLSWAMDNDRLREGLKLSQTRISKLGELPDLAAFLFKSDLGLQPAAFAGVKASPEEMLEI
LNTVQPDLEKILEWNKESIETELRACAERMGKKLKAVVAPLFVAVSGSQRSLPLFDSMELLGRSVVRQRLKVAAQVVASM
AGSGK
>Mature_484_residues
ADSAVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFEKKVLDALKWCGLEWSEGPDIGGPC
GPYRQSDRKDIYKPYVEQIVANGHGFRCFCTPERLEQMREAQRAAGKPPKYDGLCLSLSAEEVTSRVAAGEPHVVRMKIP
TEGSCKFRDGVYGDVEIPWEAVDMQVLLKADGMPTYHMANVVDDHLMKITHVARGEEWLASVPKHILIYQYLGLEPPVFM
HLSLMRNADKSKLSKRKNPTSISYYTALGYLPEALMNFLGLFFIQIAEGEELLTMAELAEKFDPENLSKAGAIFDIQKLD
WLNARWIREKLSEEQFAAKVLSWAMDNDRLREGLKLSQTRISKLGELPDLAAFLFKSDLGLQPAAFAGVKASPEEMLEIL
NTVQPDLEKILEWNKESIETELRACAERMGKKLKAVVAPLFVAVSGSQRSLPLFDSMELLGRSVVRQRLKVAAQVVASMA
GSGK

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=498, Percent_Identity=35.7429718875502, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1788743, Length=482, Percent_Identity=35.0622406639004, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI226510925, Length=263, Percent_Identity=29.277566539924, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=34.2158859470468, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6324540, Length=341, Percent_Identity=41.0557184750733, Blast_Score=254, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24665321, Length=491, Percent_Identity=36.4562118126273, Blast_Score=302, Evalue=4e-82,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54167; Mature: 54036

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSAVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFEKKV
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
LDALKWCGLEWSEGPDIGGPCGPYRQSDRKDIYKPYVEQIVANGHGFRCFCTPERLEQMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHH
EAQRAAGKPPKYDGLCLSLSAEEVTSRVAAGEPHVVRMKIPTEGSCKFRDGVYGDVEIPW
HHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCH
EAVDMQVLLKADGMPTYHMANVVDDHLMKITHVARGEEWLASVPKHILIYQYLGLEPPVF
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
MHLSLMRNADKSKLSKRKNPTSISYYTALGYLPEALMNFLGLFFIQIAEGEELLTMAELA
HHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EKFDPENLSKAGAIFDIQKLDWLNARWIREKLSEEQFAAKVLSWAMDNDRLREGLKLSQT
HHCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHH
RISKLGELPDLAAFLFKSDLGLQPAAFAGVKASPEEMLEILNTVQPDLEKILEWNKESIE
HHHHHCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHH
TELRACAERMGKKLKAVVAPLFVAVSGSQRSLPLFDSMELLGRSVVRQRLKVAAQVVASM
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGK
HCCCC
>Mature Secondary Structure 
ADSAVRVRIAPSPTGEPHVGTAYIALFNYLFAKKHGGEFILRIEDTDATRSTPEFEKKV
CCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHH
LDALKWCGLEWSEGPDIGGPCGPYRQSDRKDIYKPYVEQIVANGHGFRCFCTPERLEQMR
HHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHH
EAQRAAGKPPKYDGLCLSLSAEEVTSRVAAGEPHVVRMKIPTEGSCKFRDGVYGDVEIPW
HHHHHCCCCCCCCCEEEECCHHHHHHHHHCCCCCEEEEECCCCCCCEECCCCCCCCCCCH
EAVDMQVLLKADGMPTYHMANVVDDHLMKITHVARGEEWLASVPKHILIYQYLGLEPPVF
HHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHH
MHLSLMRNADKSKLSKRKNPTSISYYTALGYLPEALMNFLGLFFIQIAEGEELLTMAELA
HHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
EKFDPENLSKAGAIFDIQKLDWLNARWIREKLSEEQFAAKVLSWAMDNDRLREGLKLSQT
HHCCCCHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHH
RISKLGELPDLAAFLFKSDLGLQPAAFAGVKASPEEMLEILNTVQPDLEKILEWNKESIE
HHHHHCCCHHHHHHHHHHCCCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHCCHHHHH
TELRACAERMGKKLKAVVAPLFVAVSGSQRSLPLFDSMELLGRSVVRQRLKVAAQVVASM
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGK
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2661539; 11481430 [H]