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The map label for this gene is lysS [H]

Identifier: 150397998

GI number: 150397998

Start: 2922188

End: 2923684

Strand: Direct

Name: lysS [H]

Synonym: Smed_2800

Alternate gene names: 150397998

Gene position: 2922188-2923684 (Clockwise)

Preceding gene: 150397997

Following gene: 150398000

Centisome position: 77.27

GC content: 60.25

Gene sequence:

>1497_bases
ATGACCGATAAGACAGAAAACTCCGGCCTTTCCTCCGATGCGACGGAAGTACGCGCCCAGAAGCTGAAGCTCCTGCGCGA
GCAGATCGGCGATGTCTATCCCGCGCATTTCCATCGGACGATGACCAATGCGGAATTGGCCGAGAAGTACCAGGACCTCG
CGCCGGACACGGAGACGCAGGACCTCGTCACCGTTGCCGGACGAGTCTATTCCTCGCGCAATTCCGGCATGTTCATGGAC
ATCCATGACGCTTCCGGCAAGATCCAGGTCTTCTCCCACAAGGACACGACGCCTGAGGAGGCGCGTGGCCTCCTGCCGAT
GATCGACCTCGGAGATGTCATCGGCGTCACCGGGCTCGTCCGCCGCACGAAGCGCGGCGAACTGACGATCAACGCGCAGC
AGATCACCATGCTGACCAAGTCGCTCCTGCCGATGCCGGAAAAGTATCACGGCCTTGCAGACATCGAGACGCGCTATCGC
AAGCGATATCTGGACATCATGGTCAATGAGGAATCGAAGCTGCGCTTCCAGCAAAGAAGTCGCATCGTCTCCAGCCTGCG
CCGCTTCCTCGAGGACGAAGGCTTCATGGAAGTCGAGACGCCGATGCTGCAGCCGATCTACGGTGGCGCGACGGCCGAGC
CCTTCAAGACCCATCACAATACGCTGAAGCTCGATATGTATCTGCGCATCGCGCCCGAGCTTTATCTCAAGCGCGTGCTG
GTCTCCGGTCTCACCGACAAAGTCTTCGAAATCAACCGCAACTTCCGCAACGAAGGCGTCTCCACCCGGCACAATCCGGA
ATTCACCATGATGGAGTGCTATTGGGCCTATGCCGACTATGAAGACGTAATGGGCCTCGTCGAACGTATGTTCGAGACCC
TTGCTCTGGCGGTCCACGGCACGACCGAATTCGAGTTCGGCGACAAGCAGCTCTCCTTCAAGGGGCCGTTCCCGCGCGTC
TCCATGCCCGCCGCTGTCAAGGACGCGACCGGCATTGATTTCCTAGCGATCAAAAGCGACGAGGAGGCCCGACAGGCGGC
TCGCGATGCCGGTGTCGAGATCGAGAAGGACGCGACATGGGGCGAGGTGCTCGCCTTCCTCTTCGAGGAAAAGGTCGAAG
CGACGCTGATCCAGCCTGCCCACGTCATCCATTTCCCGAAGGACATCTCGCCCTTCGCCAAAGAGGTGCCGGGCGAGCCG
AGGCTCGTCGAGCGGTTCGAGACCTATTGCAACGGATGGGAGATCGGCAACGCCTTTTCCGAGCTCAACGACCCGGTCGA
GCAACGCGCTCGTATGGTCGAACAAATGGAACAGGCGCACGCACGCGGCGAGAAGGAAAAGACGCTCGACGAGGACTTCC
TCGATGCCATGGACCAGGGCATGCCGCCCGCAGGTGGTTTGGGGATCGGTGTCGACCGGCTGATCATGCTGCTCACCAAC
TCGCCGTCGATCCGCGACATCATTCTCTTCCCGGCCCGACGCCATAAGTCCGATTGA

Upstream 100 bases:

>100_bases
TCGATGGAACTGCTCGGCCGATCTGTCGTGCGTCAGCGGTTGAAGGTGGCCGCACAGGTCGTCGCCTCCATGGCAGGCAG
CGGGAAGTAAGGACGAGACC

Downstream 100 bases:

>100_bases
AGCCTGAGCCGAGCGCAGGCGGGGAGCGAGACACGGGCGGAAACCCGCGCGCTTGTCCTTCCGCTCCGGATCCAAGCGGA
CGTTAATGCCCCGCGTTCTC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MTDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQDLVTVAGRVYSSRNSGMFMD
IHDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLVRRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYR
KRYLDIMVNEESKLRFQQRSRIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVL
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHGTTEFEFGDKQLSFKGPFPRV
SMPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATWGEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEP
RLVERFETYCNGWEIGNAFSELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTN
SPSIRDIILFPARRHKSD

Sequences:

>Translated_498_residues
MTDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQDLVTVAGRVYSSRNSGMFMD
IHDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLVRRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYR
KRYLDIMVNEESKLRFQQRSRIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVL
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHGTTEFEFGDKQLSFKGPFPRV
SMPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATWGEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEP
RLVERFETYCNGWEIGNAFSELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTN
SPSIRDIILFPARRHKSD
>Mature_497_residues
TDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQDLVTVAGRVYSSRNSGMFMDI
HDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLVRRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYRK
RYLDIMVNEESKLRFQQRSRIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVLV
SGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHGTTEFEFGDKQLSFKGPFPRVS
MPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATWGEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEPR
LVERFETYCNGWEIGNAFSELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTNS
PSIRDIILFPARRHKSD

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=499, Percent_Identity=37.875751503006, Blast_Score=333, Evalue=2e-91,
Organism=Homo sapiens, GI194272210, Length=499, Percent_Identity=37.875751503006, Blast_Score=333, Evalue=2e-91,
Organism=Escherichia coli, GI1789256, Length=490, Percent_Identity=46.1224489795918, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1790571, Length=490, Percent_Identity=45.9183673469388, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI87082379, Length=325, Percent_Identity=31.3846153846154, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1788173, Length=265, Percent_Identity=29.4339622641509, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1787161, Length=478, Percent_Identity=23.6401673640167, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535927, Length=505, Percent_Identity=39.2079207920792, Blast_Score=355, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI17535925, Length=505, Percent_Identity=39.2079207920792, Blast_Score=355, Evalue=4e-98,
Organism=Caenorhabditis elegans, GI71994340, Length=497, Percent_Identity=39.0342052313883, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI32566633, Length=281, Percent_Identity=28.4697508896797, Blast_Score=89, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=507, Percent_Identity=38.2642998027613, Blast_Score=338, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6324256, Length=480, Percent_Identity=30.625, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6323011, Length=480, Percent_Identity=22.7083333333333, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=327, Percent_Identity=27.5229357798165, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24640849, Length=492, Percent_Identity=39.2276422764228, Blast_Score=350, Evalue=1e-96,
Organism=Drosophila melanogaster, GI24640851, Length=492, Percent_Identity=39.2276422764228, Blast_Score=349, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17136276, Length=459, Percent_Identity=22.4400871459695, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24584738, Length=274, Percent_Identity=26.2773722627737, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56536; Mature: 56405

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLVTVAGRVYSSRNSGMFMDIHDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHH
RRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYRKRYLDIMVNEESKLRFQQRS
HHCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHEECCHHHHHHHHHH
RIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVL
HHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCCHHHCCCEEEEEEEEEHHHHHHHHHHH
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHG
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHEEHHCCHHHHHHHHHHHHHHHHHHHCC
TTEFEFGDKQLSFKGPFPRVSMPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATW
CCEEECCCCEEECCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCH
GEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEPRLVERFETYCNGWEIGNAFS
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHH
ELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTN
HHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
SPSIRDIILFPARRHKSD
CCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TDKTENSGLSSDATEVRAQKLKLLREQIGDVYPAHFHRTMTNAELAEKYQDLAPDTETQ
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHH
DLVTVAGRVYSSRNSGMFMDIHDASGKIQVFSHKDTTPEEARGLLPMIDLGDVIGVTGLV
HHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHHH
RRTKRGELTINAQQITMLTKSLLPMPEKYHGLADIETRYRKRYLDIMVNEESKLRFQQRS
HHCCCCCEEEEHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHEECCHHHHHHHHHH
RIVSSLRRFLEDEGFMEVETPMLQPIYGGATAEPFKTHHNTLKLDMYLRIAPELYLKRVL
HHHHHHHHHHHCCCCEEECCCHHCCCCCCCCCCCHHHCCCEEEEEEEEEHHHHHHHHHHH
VSGLTDKVFEINRNFRNEGVSTRHNPEFTMMECYWAYADYEDVMGLVERMFETLALAVHG
HHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHEEHHCCHHHHHHHHHHHHHHHHHHHCC
TTEFEFGDKQLSFKGPFPRVSMPAAVKDATGIDFLAIKSDEEARQAARDAGVEIEKDATW
CCEEECCCCEEECCCCCCCCCCCCHHCCCCCCEEEEECCCHHHHHHHHHCCCEEECCCCH
GEVLAFLFEEKVEATLIQPAHVIHFPKDISPFAKEVPGEPRLVERFETYCNGWEIGNAFS
HHHHHHHHHHHHHHHHCCCHHEEECCCCCCHHHHHCCCCCHHHHHHHHHHCCCCCCHHHH
ELNDPVEQRARMVEQMEQAHARGEKEKTLDEDFLDAMDQGMPPAGGLGIGVDRLIMLLTN
HHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCC
SPSIRDIILFPARRHKSD
CCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10225420; 11481430 [H]