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The map label for this gene is emrK [H]

Identifier: 150397993

GI number: 150397993

Start: 2916292

End: 2917500

Strand: Reverse

Name: emrK [H]

Synonym: Smed_2795

Alternate gene names: 150397993

Gene position: 2917500-2916292 (Counterclockwise)

Preceding gene: 150397999

Following gene: 150397992

Centisome position: 77.14

GC content: 63.85

Gene sequence:

>1209_bases
ATGTCCGCTCCCAGCTCATCCAGCGCTGCCCGTGTCCGTCCCGTCGGCGACGATTTCGAAGTCGTGGACGATCAGAAGGC
CGAGGCCAAGAGCCCCTCGACCGACGCCGCTACCGATGAGCAGGCGGGCGAGCAGATCGCTACACCTCCTAAAAAACGTC
GCAAGATCCTTCCGGTTCTGGGCATCGTGCTCCTCGGTGCCGCCGCCTGGTACGGCTATGATTGGTGGACCAATGGGCGC
TTCCTGGTCTCGACCGACGACGCGTACATAGAAGGCGACATTGCGACGATCTCGCCGAAGGTTTCCGGCTATATTGCCAA
GGTCGACGTCGTCGCCAACCAGCATGTGAAGGCCGGCGACCCGCTCGTCACGCTCGACGACGGCGATTACCGCATCGCGG
CGGAGCAGGCGGAGGCGCAGATCGCCACACAGAAGCTGGCGCTCAGCCGTTTCGACGCACAGATCGCCGGGGCGAAGGCG
AGCCTTACTCAAACCGAAGCGCAGAAGAAGGCGCTCGAGGCGACGGTGCGCGGCGCGGAACTGGCACAGAGGCGCGCCAG
CGAACTGCAGTCGAAGTCATTCGGTACGGATGCTTCTCTCGACAGCGCCCAGGTGGCGCTCGATCAAGCCCGTGCAAATC
TCGCCGGCGCCGAGGCCAATATCGCGGCTGCCAAGGCCAACATCACCGTGCTCGAGGCCCAGCGCAACGAGGCTGAAAGC
ACGATCCATTCGCTGGAGCTTGCCCGCGACAAGGCAAGTCGCGATCTGGGCTTCACCGTGCTCAAGGCACCCTATGACGG
CGTGATAGGCAACGTCGCGGTCCAGGTCGGCGATCTCGTTTCGGCAGGCCAGCGGCTCGCCGCGCTCGTGCCCACCGATC
AGCTCTATATCGACGCCAACTTCAAGGAGACGCAGATCGCGCATCTGGTGCCGGGTTCCAAGGTTGAGATCCATGTGGAC
GCCTATGAGGATCGCCCGATCGAAGGCACCGTGGCATCGATCTCGCCGGCCTCCGGCTCGGTCTTCTCGCTGCTGCCCGC
CGAAAACGCGACGGGCAACTTCACCAAGGTCATCCAGCGCGTTCCGGTGCGCATTACCTTGCCGGCCGACGTGCTGGCCG
AGGGACATCTGCGCGCGGGTCTGAGCGTAGTCGTAGACGTCGATACACGCACTGCACCGGAACAGTCGAAGCTCGCTCAG
GCGAAGTAA

Upstream 100 bases:

>100_bases
AACCGGTTCGATTTGCCCTTGATATGCGGCGCGAAAGGCATTATGTCAAGATCCATCGAACCGAACGGTTCAGTTCAATC
TCAACCCGAATTGGTGGTCC

Downstream 100 bases:

>100_bases
AAAGGGCTGCAGGCCTGAGGAGTGGCGGAAATGGCCGCAACGGCAATAGCGGGCGCTGCTGCGCCGAGCCTACCGAAAGC
CGAGGAGCATATGGATCCGC

Product: secretion protein HlyD family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MSAPSSSSAARVRPVGDDFEVVDDQKAEAKSPSTDAATDEQAGEQIATPPKKRRKILPVLGIVLLGAAAWYGYDWWTNGR
FLVSTDDAYIEGDIATISPKVSGYIAKVDVVANQHVKAGDPLVTLDDGDYRIAAEQAEAQIATQKLALSRFDAQIAGAKA
SLTQTEAQKKALEATVRGAELAQRRASELQSKSFGTDASLDSAQVALDQARANLAGAEANIAAAKANITVLEAQRNEAES
TIHSLELARDKASRDLGFTVLKAPYDGVIGNVAVQVGDLVSAGQRLAALVPTDQLYIDANFKETQIAHLVPGSKVEIHVD
AYEDRPIEGTVASISPASGSVFSLLPAENATGNFTKVIQRVPVRITLPADVLAEGHLRAGLSVVVDVDTRTAPEQSKLAQ
AK

Sequences:

>Translated_402_residues
MSAPSSSSAARVRPVGDDFEVVDDQKAEAKSPSTDAATDEQAGEQIATPPKKRRKILPVLGIVLLGAAAWYGYDWWTNGR
FLVSTDDAYIEGDIATISPKVSGYIAKVDVVANQHVKAGDPLVTLDDGDYRIAAEQAEAQIATQKLALSRFDAQIAGAKA
SLTQTEAQKKALEATVRGAELAQRRASELQSKSFGTDASLDSAQVALDQARANLAGAEANIAAAKANITVLEAQRNEAES
TIHSLELARDKASRDLGFTVLKAPYDGVIGNVAVQVGDLVSAGQRLAALVPTDQLYIDANFKETQIAHLVPGSKVEIHVD
AYEDRPIEGTVASISPASGSVFSLLPAENATGNFTKVIQRVPVRITLPADVLAEGHLRAGLSVVVDVDTRTAPEQSKLAQ
AK
>Mature_401_residues
SAPSSSSAARVRPVGDDFEVVDDQKAEAKSPSTDAATDEQAGEQIATPPKKRRKILPVLGIVLLGAAAWYGYDWWTNGRF
LVSTDDAYIEGDIATISPKVSGYIAKVDVVANQHVKAGDPLVTLDDGDYRIAAEQAEAQIATQKLALSRFDAQIAGAKAS
LTQTEAQKKALEATVRGAELAQRRASELQSKSFGTDASLDSAQVALDQARANLAGAEANIAAAKANITVLEAQRNEAEST
IHSLELARDKASRDLGFTVLKAPYDGVIGNVAVQVGDLVSAGQRLAALVPTDQLYIDANFKETQIAHLVPGSKVEIHVDA
YEDRPIEGTVASISPASGSVFSLLPAENATGNFTKVIQRVPVRITLPADVLAEGHLRAGLSVVVDVDTRTAPEQSKLAQA
K

Specific function: Unknown

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788711, Length=359, Percent_Identity=36.2116991643454, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1789041, Length=359, Percent_Identity=34.8189415041783, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1790519, Length=320, Percent_Identity=29.0625, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI87081951, Length=307, Percent_Identity=26.7100977198697, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI1789637, Length=318, Percent_Identity=27.0440251572327, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790024, Length=250, Percent_Identity=25.2, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790012, Length=248, Percent_Identity=23.7903225806452, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42444; Mature: 42313

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPSSSSAARVRPVGDDFEVVDDQKAEAKSPSTDAATDEQAGEQIATPPKKRRKILPVL
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHH
GIVLLGAAAWYGYDWWTNGRFLVSTDDAYIEGDIATISPKVSGYIAKVDVVANQHVKAGD
HHHHHHHHHHCCEEEECCCEEEEECCCCEEECCEEEECCCCCCEEEEEEEEECCCCCCCC
PLVTLDDGDYRIAAEQAEAQIATQKLALSRFDAQIAGAKASLTQTEAQKKALEATVRGAE
CEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQRRASELQSKSFGTDASLDSAQVALDQARANLAGAEANIAAAKANITVLEAQRNEAES
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECEEEEEECCCCHHHH
TIHSLELARDKASRDLGFTVLKAPYDGVIGNVAVQVGDLVSAGQRLAALVPTDQLYIDAN
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECC
FKETQIAHLVPGSKVEIHVDAYEDRPIEGTVASISPASGSVFSLLPAENATGNFTKVIQR
CCCCEEEEECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHH
VPVRITLPADVLAEGHLRAGLSVVVDVDTRTAPEQSKLAQAK
CCEEEEECHHHHHCCCCCCCCEEEEEECCCCCCCHHHHCCCC
>Mature Secondary Structure 
SAPSSSSAARVRPVGDDFEVVDDQKAEAKSPSTDAATDEQAGEQIATPPKKRRKILPVL
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHH
GIVLLGAAAWYGYDWWTNGRFLVSTDDAYIEGDIATISPKVSGYIAKVDVVANQHVKAGD
HHHHHHHHHHCCEEEECCCEEEEECCCCEEECCEEEECCCCCCEEEEEEEEECCCCCCCC
PLVTLDDGDYRIAAEQAEAQIATQKLALSRFDAQIAGAKASLTQTEAQKKALEATVRGAE
CEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
LAQRRASELQSKSFGTDASLDSAQVALDQARANLAGAEANIAAAKANITVLEAQRNEAES
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECEEEEEECCCCHHHH
TIHSLELARDKASRDLGFTVLKAPYDGVIGNVAVQVGDLVSAGQRLAALVPTDQLYIDAN
HHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCCCEEEEECC
FKETQIAHLVPGSKVEIHVDAYEDRPIEGTVASISPASGSVFSLLPAENATGNFTKVIQR
CCCCEEEEECCCCEEEEEEECCCCCCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHHH
VPVRITLPADVLAEGHLRAGLSVVVDVDTRTAPEQSKLAQAK
CCEEEEECHHHHHCCCCCCCCEEEEEECCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]