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The map label for this gene is emrB [H]

Identifier: 150397992

GI number: 150397992

Start: 2914672

End: 2916261

Strand: Reverse

Name: emrB [H]

Synonym: Smed_2794

Alternate gene names: 150397992

Gene position: 2916261-2914672 (Counterclockwise)

Preceding gene: 150397993

Following gene: 150397990

Centisome position: 77.11

GC content: 61.89

Gene sequence:

>1590_bases
ATGGCCGCAACGGCAATAGCGGGCGCTGCTGCGCCGAGCCTACCGAAAGCCGAGGAGCATATGGATCCGCGGCGGCTCAT
CGCCTTCTTCGCGATGGTCGTCGGCATGTTCATGGCGATCCTCGACATCCAGATCGTCTCCGCCTCGCTCGCCGAAATCC
AGGCGGGTCTTTCTGCCGGGTCCGACGAGATCGGCTGGGTCCAGACGTCCTACCTGATTGCGGAAGTCATCATGATCCCG
CTCTCGGGCACGCTGGCCCGCATCGTCTCGACGCGGGTGCTCTTCTCCGTGGCTGCCGCCGGTTTTACGGCAGCCAGCGC
CCTTGCGGCTACCGCCACCAATATCGAGCAGATGATCGCCTACCGGGCGATCCAGGGCTTTATCGGCGGCGGCATGATCC
CGTCGGTCTTTGCCGCGGCCTTCACCATCTTCCCGCCCTCGAAGCGAAACGTCGTCTCGCCGATCATCGGTCTCATCGCG
ACGCTGGCGCCGACCATCGGCCCGACGGTCGGCGGCTATCTCAGCCATGCCTTCTCCTGGCACTGGCTGTTTCTCGTCAA
CGTCATCCCCGGCATCATCGTCGCGACGGTTACCTGGTTCTTCATCGACTTCGACAAGCCGGAACTCGGATTGATCAAGA
AATTCGACTGGTGGGGACTTCTCGCCATGGCGATCTTCCTCGGCTCGCTCGAATATGTTCTGGAGGAAGGCAACGCCAAC
GACTGGTTCAACGACGAGCATATCGTCACGGGCGCCGTTGCCACCACGGTCGCCGCCGTCATTTTCTTCTACCGGGCGTT
CAAGGTCGATTTCCCGGTTGTCGATCTCAAGGCCTTTGCCAACCGCAACTTCGCCTTCGGCTCGCTCTTCTCCTTCGTGA
TGGGGATCGGCCTCTACGGTCTCACCTATCTCTATCCGCTCTATCTCGGGCGCATTCGCGGCTACGATTCGCTGATGATC
GGCGAGACCATGTTCGTCTCGGGCCTCGCAATGTTCGTCACGGCGCCGGTCGCGGGCTTTCTCTCCGGCCGAATGGATCC
GCGGGCGATGATGACGATCGGCTTTGCCGGTTTTGCCGCGGGCACATGGCTGATGAGCCAGATGACTGCCGACTGGGATT
TCTGGGAACTGCTCGTCCCGCAGATCCTGCGCGGCTGCTCGCTGATGCTATGCATGGTGCCGATCAACAATATCGCGCTC
GGCACCCTGCCGCCGGCGCGCATCCGCAACGCGTCGGGCCTCTACAATCTGACGCGCAATCTCGGAGGCGCCGTTGGGCT
TGCGGTCATCAACACTATTCTCACGCAGCGCCAGGATTTTCACTACGCACGCCTTGCCGAACACGTGGAATGGGGTAATC
CGGAGGCAGTTGAGCGGATGAGAAACATCGCCTCGACGTTCACCGCGCACGGCCTCGACGGAACCACGGCCGCGGTCAAG
CAACTGGCGGCGATGGTTCAGCAGCAGGCAGTGATCATGTCCTTCATCGACGTGTTCGTATTGCTGACGGTCCTGTTCCT
GTCGATGATCGCCGGCGTTCTGATGATCGCGAAACCGCAAGCGGCGGGTCCCGGAGGTGGCGGGCACTAG

Upstream 100 bases:

>100_bases
TCTGAGCGTAGTCGTAGACGTCGATACACGCACTGCACCGGAACAGTCGAAGCTCGCTCAGGCGAAGTAAAAAGGGCTGC
AGGCCTGAGGAGTGGCGGAA

Downstream 100 bases:

>100_bases
GTGTTTCGCGGCATGGAGAGCGGCGCCTCCGCCGCCGCGGTTTTTCCTTTTCCTTATGATCGTGATTCCGCATTTGCGGA
ACTCAGGATTTCCGGATTTC

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAATAIAGAAAPSLPKAEEHMDPRRLIAFFAMVVGMFMAILDIQIVSASLAEIQAGLSAGSDEIGWVQTSYLIAEVIMIP
LSGTLARIVSTRVLFSVAAAGFTAASALAATATNIEQMIAYRAIQGFIGGGMIPSVFAAAFTIFPPSKRNVVSPIIGLIA
TLAPTIGPTVGGYLSHAFSWHWLFLVNVIPGIIVATVTWFFIDFDKPELGLIKKFDWWGLLAMAIFLGSLEYVLEEGNAN
DWFNDEHIVTGAVATTVAAVIFFYRAFKVDFPVVDLKAFANRNFAFGSLFSFVMGIGLYGLTYLYPLYLGRIRGYDSLMI
GETMFVSGLAMFVTAPVAGFLSGRMDPRAMMTIGFAGFAAGTWLMSQMTADWDFWELLVPQILRGCSLMLCMVPINNIAL
GTLPPARIRNASGLYNLTRNLGGAVGLAVINTILTQRQDFHYARLAEHVEWGNPEAVERMRNIASTFTAHGLDGTTAAVK
QLAAMVQQQAVIMSFIDVFVLLTVLFLSMIAGVLMIAKPQAAGPGGGGH

Sequences:

>Translated_529_residues
MAATAIAGAAAPSLPKAEEHMDPRRLIAFFAMVVGMFMAILDIQIVSASLAEIQAGLSAGSDEIGWVQTSYLIAEVIMIP
LSGTLARIVSTRVLFSVAAAGFTAASALAATATNIEQMIAYRAIQGFIGGGMIPSVFAAAFTIFPPSKRNVVSPIIGLIA
TLAPTIGPTVGGYLSHAFSWHWLFLVNVIPGIIVATVTWFFIDFDKPELGLIKKFDWWGLLAMAIFLGSLEYVLEEGNAN
DWFNDEHIVTGAVATTVAAVIFFYRAFKVDFPVVDLKAFANRNFAFGSLFSFVMGIGLYGLTYLYPLYLGRIRGYDSLMI
GETMFVSGLAMFVTAPVAGFLSGRMDPRAMMTIGFAGFAAGTWLMSQMTADWDFWELLVPQILRGCSLMLCMVPINNIAL
GTLPPARIRNASGLYNLTRNLGGAVGLAVINTILTQRQDFHYARLAEHVEWGNPEAVERMRNIASTFTAHGLDGTTAAVK
QLAAMVQQQAVIMSFIDVFVLLTVLFLSMIAGVLMIAKPQAAGPGGGGH
>Mature_528_residues
AATAIAGAAAPSLPKAEEHMDPRRLIAFFAMVVGMFMAILDIQIVSASLAEIQAGLSAGSDEIGWVQTSYLIAEVIMIPL
SGTLARIVSTRVLFSVAAAGFTAASALAATATNIEQMIAYRAIQGFIGGGMIPSVFAAAFTIFPPSKRNVVSPIIGLIAT
LAPTIGPTVGGYLSHAFSWHWLFLVNVIPGIIVATVTWFFIDFDKPELGLIKKFDWWGLLAMAIFLGSLEYVLEEGNAND
WFNDEHIVTGAVATTVAAVIFFYRAFKVDFPVVDLKAFANRNFAFGSLFSFVMGIGLYGLTYLYPLYLGRIRGYDSLMIG
ETMFVSGLAMFVTAPVAGFLSGRMDPRAMMTIGFAGFAAGTWLMSQMTADWDFWELLVPQILRGCSLMLCMVPINNIALG
TLPPARIRNASGLYNLTRNLGGAVGLAVINTILTQRQDFHYARLAEHVEWGNPEAVERMRNIASTFTAHGLDGTTAAVKQ
LAAMVQQQAVIMSFIDVFVLLTVLFLSMIAGVLMIAKPQAAGPGGGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=28.9738430583501, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1788710, Length=499, Percent_Identity=27.0541082164329, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1790195, Length=433, Percent_Identity=25.1732101616628, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI87081983, Length=425, Percent_Identity=25.4117647058824, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=25.6218905472637, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=26.1904761904762, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56846; Mature: 56715

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATAIAGAAAPSLPKAEEHMDPRRLIAFFAMVVGMFMAILDIQIVSASLAEIQAGLSAG
CCCCHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SDEIGWVQTSYLIAEVIMIPLSGTLARIVSTRVLFSVAAAGFTAASALAATATNIEQMIA
CCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YRAIQGFIGGGMIPSVFAAAFTIFPPSKRNVVSPIIGLIATLAPTIGPTVGGYLSHAFSW
HHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
HWLFLVNVIPGIIVATVTWFFIDFDKPELGLIKKFDWWGLLAMAIFLGSLEYVLEEGNAN
HHHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCC
DWFNDEHIVTGAVATTVAAVIFFYRAFKVDFPVVDLKAFANRNFAFGSLFSFVMGIGLYG
CCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHCCCCHHHHHHHHHHHHHHHH
LTYLYPLYLGRIRGYDSLMIGETMFVSGLAMFVTAPVAGFLSGRMDPRAMMTIGFAGFAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHH
GTWLMSQMTADWDFWELLVPQILRGCSLMLCMVPINNIALGTLPPARIRNASGLYNLTRN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHCCCCHHHHHHHH
LGGAVGLAVINTILTQRQDFHYARLAEHVEWGNPEAVERMRNIASTFTAHGLDGTTAAVK
HCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
QLAAMVQQQAVIMSFIDVFVLLTVLFLSMIAGVLMIAKPQAAGPGGGGH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
AATAIAGAAAPSLPKAEEHMDPRRLIAFFAMVVGMFMAILDIQIVSASLAEIQAGLSAG
CCCHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]