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The map label for this gene is glgB [H]

Identifier: 150397938

GI number: 150397938

Start: 2854543

End: 2856753

Strand: Direct

Name: glgB [H]

Synonym: Smed_2740

Alternate gene names: 150397938

Gene position: 2854543-2856753 (Clockwise)

Preceding gene: 150397937

Following gene: 150397939

Centisome position: 75.48

GC content: 62.1

Gene sequence:

>2211_bases
ATGACGGTTCCCCCCGGCCAGGATCCAGCTCCCGCCCCGTCAGGCACGCTGCCAGCGCCGGAAATTGCGGCAATACTTTC
CGGCACCCATAGCGACCCCTTTGCCGTCCTTGGAGTCCACGCCGCGGGCAAGACATTTGTTGCCCGATGCTTCATTCCCG
GAGCGGAGACGGTCGTTGCGGAGACGCTTGCCGGGAACGAACTCGGCACGCTCGTGCGGCGCGACGAGGCCGGCTTCTTC
GAGGGGCCGGTGGCCTTGAGCAAGGAGCAACCCGTCCGCTACCGCGCTCGCAATGGCGGCGGCGAATGGACGGTCATCGA
TCCCTACAGCTTCGGTCCCGTGCTCGGGCCGATGGACGATTACTATATCCGCGAGGGCTCGCATCTTCGCCTCTTCGACA
AGATGGGTGCGCATCCGATCTCGCATGAAGGCGCGGAGGGCTTTCACTTCGCCGTCTGGGCGCCGAACGCGCGGCGCGTC
TCCGTCGTCGGGAACTTCAATGAATGGGACGGGCGCCGGCACGTGATGCGCCTGCGCGTGGACACCGGTATTTGGGAAAT
CTTCATACCCGGCGTGCCGGTCGGCACACCCTACAAGTACGAAATTGTCGACCGTCAAGGCACGCTCTTGCCGTTGAAAG
CCGACCCTTTTGCCCGCCGGTCGGAACTCAGGCCCGACACGGCTTCCATGACGGCGGCCGAAATCGCTCAGGTTTGGGAA
GATGAGGCGCATCGGCGCCACTGGGCCGAGCTCGATCCGCGCCGCCAGCCGATCTCGATCTACGAGGTCCACGCCGCCTC
ATGGCAGCGTCGCGACAATGGCGACATGCTCTCCTGGGACGAGCTGGCGGAAAGGCTGATCCCCTATTGCGTCGACATGG
GCTTCACCCATATCGAGTTCCTGCCCATTTCGGAATATCCCTATGACCCGTCCTGGGGCTACCAGACCACCGGCCTGTAT
GCGCCGACCGCACGCTTCGGCGAGCCGGAGGGCTTTGCGCGCTTCGTCAACGGTTGCCACAAGGTCGGCCTCGGCGTCAT
CCTCGACTGGGTGCCGGCGCATTTCCCGACGGACGAACACGGGCTGCGCTGGTTCGACGGCACCGCCCTCTACGAGCATG
CGGATCCACGCCAGGGCTTTCACCCCGACTGGAACACTGCAATCTACAATTTCGGCCGTCAGGAGGTCGTCGCCTTTCTG
GTCAACAACGCCCTCTACTGGGCGGAGAAATTCCATGTCGACGGCCTGCGCGTCGATGCGGTCGCTTCGATGCTTTATCT
CGACTATTCGCGAAAGCATGGCGAATGGGTGCCGAACGAATATGGCGGCAACGAAAACCTCGAGGCCGTGCGTTTCCTGC
AGACGATGAATTCCAGCATATACGGTATTCACCAGGGCGTGCTGACCATCGCCGAGGAGTCGACCTCCTGGCCGAAGGTC
TCGCACCCGGTGCATGCGGGCGGCCTCGGCTTCGGGTTCAAGTGGAACATGGGCTTCATGCATGACACGCTGCAATATCT
CGCGCGCGAGCCCGTTCATCGGAAGTTCCACCATAACGACCTGACTTTCGGGCTGCTCTATGCCTTCAGCGAAAACTTCG
TGCTGCCGCTCTCTCATGACGAGGTCGTGCACGGCAAGGGATCGCTGATCGCCAAGATGGCCGGCGATGACTGGCAGAAA
TTCGCCAATCTCAGGGCCTATTACGGTTTCATGTGGGGCTATCCCGGCAAGAAGCTGCTTTTCATGGGACAGGAATTCGC
GCAGTGGCGAGAGTGGTCTGAGGACCGCGCACTCGACTGGAACCTGCTCGAATACAATCTGCACGAAGGAATGCGCCGCC
TGGTGCGCGACCTGAACGGTACCTACCGCTCGAAGCCGGCGCTGCACGCGCGCGACTGCGAGGGCGACGGATTCGAGTGG
CTGATTGCCGATGACCGTCAAAATTCCGTCTTCGCCTGGCTCAGGAAAGCACCCGGTGAGAAGCTCGTCGCCGTCATCAC
CAATTTCACTCCCGTCTACCGCGAACATTACGACATACCGCTTCCGGTCGCCGGGCGCTGGAAGGAAATCCTCAACACCG
ACGCGGAAATCTACGGAGGAAGCGGCAAGGGTAACGGCGGCGCCGTCATTGCGGAAAAGAGAGCGAACGGAGAGACAATC
GCCACCATCACGCTGCCGCCTCTGGCGGCACTGATGCTGGAGCAGGATTAG

Upstream 100 bases:

>100_bases
CCGCCTGGTATTCGAAGGCGGTCCGCAATACCGCCCGGATGGGCTGGTTCTCATCCGATCGAACGATCCGGCAATATGCC
GGAGAGATCTGGAGAGCCGG

Downstream 100 bases:

>100_bases
AGGTCCCGCCCGGCTGGAAATGGCACGTCGGGCGGAGCAGGAGTAAGGGCTGCGAGCGTCGGAAAGAGCACGCCGGGCAG
TCCTCCGAAATGACTTTGGG

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 736; Mature: 735

Protein sequence:

>736_residues
MTVPPGQDPAPAPSGTLPAPEIAAILSGTHSDPFAVLGVHAAGKTFVARCFIPGAETVVAETLAGNELGTLVRRDEAGFF
EGPVALSKEQPVRYRARNGGGEWTVIDPYSFGPVLGPMDDYYIREGSHLRLFDKMGAHPISHEGAEGFHFAVWAPNARRV
SVVGNFNEWDGRRHVMRLRVDTGIWEIFIPGVPVGTPYKYEIVDRQGTLLPLKADPFARRSELRPDTASMTAAEIAQVWE
DEAHRRHWAELDPRRQPISIYEVHAASWQRRDNGDMLSWDELAERLIPYCVDMGFTHIEFLPISEYPYDPSWGYQTTGLY
APTARFGEPEGFARFVNGCHKVGLGVILDWVPAHFPTDEHGLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRQEVVAFL
VNNALYWAEKFHVDGLRVDAVASMLYLDYSRKHGEWVPNEYGGNENLEAVRFLQTMNSSIYGIHQGVLTIAEESTSWPKV
SHPVHAGGLGFGFKWNMGFMHDTLQYLAREPVHRKFHHNDLTFGLLYAFSENFVLPLSHDEVVHGKGSLIAKMAGDDWQK
FANLRAYYGFMWGYPGKKLLFMGQEFAQWREWSEDRALDWNLLEYNLHEGMRRLVRDLNGTYRSKPALHARDCEGDGFEW
LIADDRQNSVFAWLRKAPGEKLVAVITNFTPVYREHYDIPLPVAGRWKEILNTDAEIYGGSGKGNGGAVIAEKRANGETI
ATITLPPLAALMLEQD

Sequences:

>Translated_736_residues
MTVPPGQDPAPAPSGTLPAPEIAAILSGTHSDPFAVLGVHAAGKTFVARCFIPGAETVVAETLAGNELGTLVRRDEAGFF
EGPVALSKEQPVRYRARNGGGEWTVIDPYSFGPVLGPMDDYYIREGSHLRLFDKMGAHPISHEGAEGFHFAVWAPNARRV
SVVGNFNEWDGRRHVMRLRVDTGIWEIFIPGVPVGTPYKYEIVDRQGTLLPLKADPFARRSELRPDTASMTAAEIAQVWE
DEAHRRHWAELDPRRQPISIYEVHAASWQRRDNGDMLSWDELAERLIPYCVDMGFTHIEFLPISEYPYDPSWGYQTTGLY
APTARFGEPEGFARFVNGCHKVGLGVILDWVPAHFPTDEHGLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRQEVVAFL
VNNALYWAEKFHVDGLRVDAVASMLYLDYSRKHGEWVPNEYGGNENLEAVRFLQTMNSSIYGIHQGVLTIAEESTSWPKV
SHPVHAGGLGFGFKWNMGFMHDTLQYLAREPVHRKFHHNDLTFGLLYAFSENFVLPLSHDEVVHGKGSLIAKMAGDDWQK
FANLRAYYGFMWGYPGKKLLFMGQEFAQWREWSEDRALDWNLLEYNLHEGMRRLVRDLNGTYRSKPALHARDCEGDGFEW
LIADDRQNSVFAWLRKAPGEKLVAVITNFTPVYREHYDIPLPVAGRWKEILNTDAEIYGGSGKGNGGAVIAEKRANGETI
ATITLPPLAALMLEQD
>Mature_735_residues
TVPPGQDPAPAPSGTLPAPEIAAILSGTHSDPFAVLGVHAAGKTFVARCFIPGAETVVAETLAGNELGTLVRRDEAGFFE
GPVALSKEQPVRYRARNGGGEWTVIDPYSFGPVLGPMDDYYIREGSHLRLFDKMGAHPISHEGAEGFHFAVWAPNARRVS
VVGNFNEWDGRRHVMRLRVDTGIWEIFIPGVPVGTPYKYEIVDRQGTLLPLKADPFARRSELRPDTASMTAAEIAQVWED
EAHRRHWAELDPRRQPISIYEVHAASWQRRDNGDMLSWDELAERLIPYCVDMGFTHIEFLPISEYPYDPSWGYQTTGLYA
PTARFGEPEGFARFVNGCHKVGLGVILDWVPAHFPTDEHGLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRQEVVAFLV
NNALYWAEKFHVDGLRVDAVASMLYLDYSRKHGEWVPNEYGGNENLEAVRFLQTMNSSIYGIHQGVLTIAEESTSWPKVS
HPVHAGGLGFGFKWNMGFMHDTLQYLAREPVHRKFHHNDLTFGLLYAFSENFVLPLSHDEVVHGKGSLIAKMAGDDWQKF
ANLRAYYGFMWGYPGKKLLFMGQEFAQWREWSEDRALDWNLLEYNLHEGMRRLVRDLNGTYRSKPALHARDCEGDGFEWL
IADDRQNSVFAWLRKAPGEKLVAVITNFTPVYREHYDIPLPVAGRWKEILNTDAEIYGGSGKGNGGAVIAEKRANGETIA
TITLPPLAALMLEQD

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=646, Percent_Identity=25.3869969040248, Blast_Score=164, Evalue=3e-40,
Organism=Escherichia coli, GI1789839, Length=719, Percent_Identity=53.6856745479833, Blast_Score=752, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=410, Percent_Identity=26.0975609756098, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI32564391, Length=302, Percent_Identity=26.4900662251656, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6320826, Length=375, Percent_Identity=27.4666666666667, Blast_Score=146, Evalue=1e-35,
Organism=Drosophila melanogaster, GI28573410, Length=374, Percent_Identity=32.3529411764706, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 82998; Mature: 82867

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVPPGQDPAPAPSGTLPAPEIAAILSGTHSDPFAVLGVHAAGKTFVARCFIPGAETVVA
CCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHH
ETLAGNELGTLVRRDEAGFFEGPVALSKEQPVRYRARNGGGEWTVIDPYSFGPVLGPMDD
HHHCCCHHHHHEEECCCCCCCCCEECCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
YYIREGSHLRLFDKMGAHPISHEGAEGFHFAVWAPNARRVSVVGNFNEWDGRRHVMRLRV
CEECCCCEEEEHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEEE
DTGIWEIFIPGVPVGTPYKYEIVDRQGTLLPLKADPFARRSELRPDTASMTAAEIAQVWE
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHH
DEAHRRHWAELDPRRQPISIYEVHAASWQRRDNGDMLSWDELAERLIPYCVDMGFTHIEF
HHHHHHHHHCCCCCCCCCEEEEEECHHCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
LPISEYPYDPSWGYQTTGLYAPTARFGEPEGFARFVNGCHKVGLGVILDWVPAHFPTDEH
EECCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
GLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRQEVVAFLVNNALYWAEKFHVDGLRVDA
CCEEECCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHEEHHHHCCCCEEHHH
VASMLYLDYSRKHGEWVPNEYGGNENLEAVRFLQTMNSSIYGIHQGVLTIAEESTSWPKV
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCEEEEEECCCCCCCC
SHPVHAGGLGFGFKWNMGFMHDTLQYLAREPVHRKFHHNDLTFGLLYAFSENFVLPLSHD
CCCCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCEEEECCCC
EVVHGKGSLIAKMAGDDWQKFANLRAYYGFMWGYPGKKLLFMGQEFAQWREWSEDRALDW
CEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCC
NLLEYNLHEGMRRLVRDLNGTYRSKPALHARDCEGDGFEWLIADDRQNSVFAWLRKAPGE
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCC
KLVAVITNFTPVYREHYDIPLPVAGRWKEILNTDAEIYGGSGKGNGGAVIAEKRANGETI
EEEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCEE
ATITLPPLAALMLEQD
EEEECCHHHHHHCCCC
>Mature Secondary Structure 
TVPPGQDPAPAPSGTLPAPEIAAILSGTHSDPFAVLGVHAAGKTFVARCFIPGAETVVA
CCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEECCCCHHHHH
ETLAGNELGTLVRRDEAGFFEGPVALSKEQPVRYRARNGGGEWTVIDPYSFGPVLGPMDD
HHHCCCHHHHHEEECCCCCCCCCEECCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCC
YYIREGSHLRLFDKMGAHPISHEGAEGFHFAVWAPNARRVSVVGNFNEWDGRRHVMRLRV
CEECCCCEEEEHHHHCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCEEEEEEEE
DTGIWEIFIPGVPVGTPYKYEIVDRQGTLLPLKADPFARRSELRPDTASMTAAEIAQVWE
CCCEEEEEECCCCCCCCEEEEEECCCCCEEEECCCCCHHHHCCCCCHHHHHHHHHHHHHH
DEAHRRHWAELDPRRQPISIYEVHAASWQRRDNGDMLSWDELAERLIPYCVDMGFTHIEF
HHHHHHHHHCCCCCCCCCEEEEEECHHCCCCCCCCEECHHHHHHHHHHHHHHCCCCEEEE
LPISEYPYDPSWGYQTTGLYAPTARFGEPEGFARFVNGCHKVGLGVILDWVPAHFPTDEH
EECCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCC
GLRWFDGTALYEHADPRQGFHPDWNTAIYNFGRQEVVAFLVNNALYWAEKFHVDGLRVDA
CCEEECCCEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHEEHHHHCCCCEEHHH
VASMLYLDYSRKHGEWVPNEYGGNENLEAVRFLQTMNSSIYGIHQGVLTIAEESTSWPKV
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHCEEEEEECCCCCCCC
SHPVHAGGLGFGFKWNMGFMHDTLQYLAREPVHRKFHHNDLTFGLLYAFSENFVLPLSHD
CCCCCCCCCCCCEEECCCHHHHHHHHHHHCCHHHHHCCCCCCEEEEEEECCCEEEECCCC
EVVHGKGSLIAKMAGDDWQKFANLRAYYGFMWGYPGKKLLFMGQEFAQWREWSEDRALDW
CEECCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHCCCCCCCCC
NLLEYNLHEGMRRLVRDLNGTYRSKPALHARDCEGDGFEWLIADDRQNSVFAWLRKAPGE
EEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCC
KLVAVITNFTPVYREHYDIPLPVAGRWKEILNTDAEIYGGSGKGNGGAVIAEKRANGETI
EEEEEECCCCHHHHHCCCCCCCCCHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCEE
ATITLPPLAALMLEQD
EEEECCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]