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The map label for this gene is mltB [H]

Identifier: 150397906

GI number: 150397906

Start: 2816713

End: 2817930

Strand: Direct

Name: mltB [H]

Synonym: Smed_2708

Alternate gene names: 150397906

Gene position: 2816713-2817930 (Clockwise)

Preceding gene: 150397904

Following gene: 150397907

Centisome position: 74.48

GC content: 60.43

Gene sequence:

>1218_bases
ATGAGCACAAACCGCAGGACGTTCTTCCGACATATAGCCGCCGCTCTCGTCGTTGCCGCCGCTTTTGGGTCGTCGCCAGC
GCGCGCGGATCAAGGTTTTCAAAACTGGATCAACAACTTCTATGCGACGGCTGCCAAGAGCGGCATCAGCCAGGCAACCT
ATCGCAAGGCCTTTGCCGGTGTGAAGACGCCTGATCAGGCCGTGCTGGAGAAGGCGGCCTATCAGCCTGAGTTCAAGCAC
AAGATCTGGGAGTATATCGACGCCCGTGTCAATCCGTACACCAAGCGGACGGGACAGGAAATGGCGGCGAAGCATGCACG
CACGCTCAACGCTCTCGAGCGGCACTACGGCGTCGACAAGTCCGTTCTGCTGGCCATCTGGTCTATGGAATCGAATTACG
GCGCGATCCTCGAAAAAGACGATCGGCTGCATTACGTGCCGCGGGCGCTGGCGACGCTTGCCTATGCCGATAAGAAGCGG
GCCAAATTCGCCCGTACACAACTTATTGCGGCATTGAAGATCCTGCAGAGCGGCGATATCACCCCGCGAGAACTGACGGG
CTCCTGGGCGGGTGCCATGGGGCACACGCAGTTCATTCCGACGAGCTACCTGCTCTATGCCGTCGACGTCGACGGGAACG
GACATAGAGACATCTGGAACTCGGTGCCGGATGCCCTGGCGACGGCTGCCAATCTCTTGCGAAAGAACGGATGGAGATCG
GGCGAGACTTGGGGTTACGAGATCGTCCCGCCGGCAAATGCGGCGCAATATTCAGGCCAGACGAAAACGCTTGGCCAGTG
GGCAGCCCTTGGCTTTGCCCGCCCGAATGGTAAGGGTTTCAGCAATTCGAAGATGCGTGCGGTCCTGAAAATGCCCGGCG
GCAGCAACGGCCCCGGCTTCCTGGTGACCAAGAATTTCTTCGTCATCAAGCGCTACAACGCCTCCGACTCCTACGCGCTC
GGCGTCGGCCTCCTTGCCGATCAGATCGCAGGTTATGCGGGCATGCAGCAGCGCTGGCCGCGTCCGGACGGCTCGCTCGA
CATCAGCGAAAAGTTCGAGCTGCAGAACCGGCTGAAGGAACTCGGCTATTACGATGGCGAAGTCGACGGCAATTTCGGCT
CGGGTTCGAAGGCCGCGATCGAGGCGTTCCAGGCACAGAACGGCCTGAAACCGGACGGGCAGCCGACACAGCATCTCCTG
CGCGCCCTGCGCCGGTAA

Upstream 100 bases:

>100_bases
CTTTCCAGTGGCCTGGTGCGGGCAAATTGCGAACCTCGGAGTAGTTGGTCCGGCATGAACGCTGGCTGAACGCTCCACCG
AGAAACGGAACCGACAGCAG

Downstream 100 bases:

>100_bases
ACGCACCTGTCGCTTTTTTCCCGGTCACGGCGGTTTCCCAACAAACGGTGCCAATCGCCGTGATTTGGCGTATGCTTCCG
GGATTGTCTGCATTCGGCGG

Product: lytic murein transglycosylase

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSTNRRTFFRHIAAALVVAAAFGSSPARADQGFQNWINNFYATAAKSGISQATYRKAFAGVKTPDQAVLEKAAYQPEFKH
KIWEYIDARVNPYTKRTGQEMAAKHARTLNALERHYGVDKSVLLAIWSMESNYGAILEKDDRLHYVPRALATLAYADKKR
AKFARTQLIAALKILQSGDITPRELTGSWAGAMGHTQFIPTSYLLYAVDVDGNGHRDIWNSVPDALATAANLLRKNGWRS
GETWGYEIVPPANAAQYSGQTKTLGQWAALGFARPNGKGFSNSKMRAVLKMPGGSNGPGFLVTKNFFVIKRYNASDSYAL
GVGLLADQIAGYAGMQQRWPRPDGSLDISEKFELQNRLKELGYYDGEVDGNFGSGSKAAIEAFQAQNGLKPDGQPTQHLL
RALRR

Sequences:

>Translated_405_residues
MSTNRRTFFRHIAAALVVAAAFGSSPARADQGFQNWINNFYATAAKSGISQATYRKAFAGVKTPDQAVLEKAAYQPEFKH
KIWEYIDARVNPYTKRTGQEMAAKHARTLNALERHYGVDKSVLLAIWSMESNYGAILEKDDRLHYVPRALATLAYADKKR
AKFARTQLIAALKILQSGDITPRELTGSWAGAMGHTQFIPTSYLLYAVDVDGNGHRDIWNSVPDALATAANLLRKNGWRS
GETWGYEIVPPANAAQYSGQTKTLGQWAALGFARPNGKGFSNSKMRAVLKMPGGSNGPGFLVTKNFFVIKRYNASDSYAL
GVGLLADQIAGYAGMQQRWPRPDGSLDISEKFELQNRLKELGYYDGEVDGNFGSGSKAAIEAFQAQNGLKPDGQPTQHLL
RALRR
>Mature_404_residues
STNRRTFFRHIAAALVVAAAFGSSPARADQGFQNWINNFYATAAKSGISQATYRKAFAGVKTPDQAVLEKAAYQPEFKHK
IWEYIDARVNPYTKRTGQEMAAKHARTLNALERHYGVDKSVLLAIWSMESNYGAILEKDDRLHYVPRALATLAYADKKRA
KFARTQLIAALKILQSGDITPRELTGSWAGAMGHTQFIPTSYLLYAVDVDGNGHRDIWNSVPDALATAANLLRKNGWRSG
ETWGYEIVPPANAAQYSGQTKTLGQWAALGFARPNGKGFSNSKMRAVLKMPGGSNGPGFLVTKNFFVIKRYNASDSYALG
VGLLADQIAGYAGMQQRWPRPDGSLDISEKFELQNRLKELGYYDGEVDGNFGSGSKAAIEAFQAQNGLKPDGQPTQHLLR
ALRR

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=242, Percent_Identity=30.5785123966942, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 44606; Mature: 44475

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTNRRTFFRHIAAALVVAAAFGSSPARADQGFQNWINNFYATAAKSGISQATYRKAFAG
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKTPDQAVLEKAAYQPEFKHKIWEYIDARVNPYTKRTGQEMAAKHARTLNALERHYGVDK
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVLLAIWSMESNYGAILEKDDRLHYVPRALATLAYADKKRAKFARTQLIAALKILQSGDI
HHHHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPRELTGSWAGAMGHTQFIPTSYLLYAVDVDGNGHRDIWNSVPDALATAANLLRKNGWRS
CHHHHCCCHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GETWGYEIVPPANAAQYSGQTKTLGQWAALGFARPNGKGFSNSKMRAVLKMPGGSNGPGF
CCCCCEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCE
LVTKNFFVIKRYNASDSYALGVGLLADQIAGYAGMQQRWPRPDGSLDISEKFELQNRLKE
EEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHH
LGYYDGEVDGNFGSGSKAAIEAFQAQNGLKPDGQPTQHLLRALRR
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
STNRRTFFRHIAAALVVAAAFGSSPARADQGFQNWINNFYATAAKSGISQATYRKAFAG
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VKTPDQAVLEKAAYQPEFKHKIWEYIDARVNPYTKRTGQEMAAKHARTLNALERHYGVDK
CCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SVLLAIWSMESNYGAILEKDDRLHYVPRALATLAYADKKRAKFARTQLIAALKILQSGDI
HHHHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPRELTGSWAGAMGHTQFIPTSYLLYAVDVDGNGHRDIWNSVPDALATAANLLRKNGWRS
CHHHHCCCHHCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
GETWGYEIVPPANAAQYSGQTKTLGQWAALGFARPNGKGFSNSKMRAVLKMPGGSNGPGF
CCCCCEEECCCCCHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCE
LVTKNFFVIKRYNASDSYALGVGLLADQIAGYAGMQQRWPRPDGSLDISEKFELQNRLKE
EEECCEEEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHH
LGYYDGEVDGNFGSGSKAAIEAFQAQNGLKPDGQPTQHLLRALRR
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]