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The map label for this gene is yfkN [H]

Identifier: 150397888

GI number: 150397888

Start: 2794291

End: 2796174

Strand: Direct

Name: yfkN [H]

Synonym: Smed_2690

Alternate gene names: 150397888

Gene position: 2794291-2796174 (Clockwise)

Preceding gene: 150397887

Following gene: 150397889

Centisome position: 73.89

GC content: 62.79

Gene sequence:

>1884_bases
ATGATCAGACACATGCGGGTCGGCTTTACAGCTGTTTCCCTTTTTGCGCTCTCGTCGGGCGCAGCCTTTGCCGATTATGA
ATTGAACATCCTGCACATCAATGACCTTCATTCGCGCATCGAATCGATCAACAAGTTCGATTCGACCTGCTCCGTCGAGG
AAGAGGGCAAGAACGAGTGCTTCGGCGGCGTCGCGCGCCTCAAGACCCTGATCGACCAGAAGCGACAGGAACGGACGGGC
AAGAACCTGCTTCTGCTCAACGCCGGCGACAATTTCCAGGGTTCGCTCTTCTTCACCACCTATAAGGGCGCCGCGGAGGC
GGAATTCCTGAACCTGATGAAGTTCGACGCGATGACCGTCGGCAATCACGAGTTCGATGAAAGCGAGGACGGGCTTGCGA
GCTTCCTCGACAAGGTCACCTTCCCCGTCGTCACCGCCAATGTCCTGCCGAGTCATAAGTCAAAGATCGGCGACCGCATC
AAGCCGTCGATCGTTCTCGATGTCGGCGGCCAGAAGGTGGGTATCGTCGGCGCGGTGGCCAATGACACGCCGGAACTCGC
CTCACCCGGCCCCGACATCCTCATCGGCGAGGATGTCGCGACGATCACCAGCGCAGTCGAGGAATTGAAGAAGCAGGGCA
TCAACAAGATCGTCGCCCTGACCCATGTCGGCTATCCGCGCGACCTGGCGGCAATTGCGAAGATCCCTGATGTCGACGTC
GTGGTCGGCGGTCATTCCCACAGCCTCTTGTCCAACACCGACGAAAAGGCCGAGGGGCCCTATCCTACGATGGTCGACAA
CCCCGGCGGCTACAAGGTGCCGGTCGTACAGGCCGGCTCCTACAGCAAATATCTCGGCGACCTCATGGTGACGTTCGACG
ACAACGGCGTGGTCAAAGCAGCCAAAGGCGATCCGATCCTCGTCGATTCCTCCGTAAAGCCGGACGAAACGGTGGTCGCC
CGCGTCAAGGAGCTGGCCAAGCCGATCGAGGAACTGAGAACGAAGGTCATCGGCAAGACCGAGGCGCCGATCGACGGGTC
GCGCGAGAGCTGCCGCGCCAAGGAATGCGAAATGGGCAGCCTGGTGGCCGACGCGATGCTCGACCGCGTCAAGGGACAGG
GCGTGACGGTCGCCATCACCAATGGCGGCGGCTTGCGCGCTTCGATCGACGGCGGCGACGTGACGATGGGCGAGGTCATC
ACCGTTCTGCCCTTCCAGAACACGCTGTCCACCTTCCAGCTCAAGGGCGCGGACATCCGGGCAGCTCTTGAAAACGGACT
AAGCCAGGTCGAAGAGGGCGGCGGGCGCTTCCCGCAGGTCGCCGGTCTCAGATTTTCCTTCGATCGTTCGAAGCCCGCCG
GCAGCCGCCTCGTCAGCGTCGAGGTCGAAGACGGCGGGGCCTTTGCCGCGCTCGACCCGGAAAAGACCTATTCGCTCGTC
AGCAACAATTTCATGCGCGGCGGCGGTGACGGTTACGCGGTCTTCAAGGACAAGGGAGAGAACGCCTACGACTACGGTCC
GGGCCTCGAAACGGTGCTCGCCGATTATCTCGCCGCCCATCAACCTTTCAAACCCTACACCAGCGGCCGCATCACGGAAG
TGACTGCCGCGGCGGATGCGGCGCCCGGCGCTGCGAGTGAGCCGGAAGCCGCAGCCGCGCCGGCGGAAAGCGCAACGCCC
ACTCCTGCCCCCGAGCCCTCGACCGAGTCGGCCGCAGCCGCAGGTCCGCGCACGCACGTCATCGCCAGAGGCGACACGCT
CTGGGACCTCGCCCAGACCTTCTACGGCAGCGGCGCGGAATGGAAGAGAATATCTGCCGCCAATGGCGATCCGGCACCGC
GGGCGCTGGAAATCGGCCGAGAGCTGGAGATCCCGGCCGAATAA

Upstream 100 bases:

>100_bases
ATCCGCTGGAGCGGGACGAGGAAAAGTCTGTGCGGTTTTCCACCCGCATCCCGCCGCGCATCCTATCCGGAACTCGGCCG
GCAGCACACAGGAGCACACC

Downstream 100 bases:

>100_bases
GACGATTTGCCTCGGCATTTCCCACCGGCGCGGGCTTTCCCGCGCCGGTATTTCTTTTTAGAAAGGTTTGAAATTGTAGG
CAGCCGCGCAGTAACGTGGC

Product: 5'-nucleotidase domain-containing protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 627; Mature: 627

Protein sequence:

>627_residues
MIRHMRVGFTAVSLFALSSGAAFADYELNILHINDLHSRIESINKFDSTCSVEEEGKNECFGGVARLKTLIDQKRQERTG
KNLLLLNAGDNFQGSLFFTTYKGAAEAEFLNLMKFDAMTVGNHEFDESEDGLASFLDKVTFPVVTANVLPSHKSKIGDRI
KPSIVLDVGGQKVGIVGAVANDTPELASPGPDILIGEDVATITSAVEELKKQGINKIVALTHVGYPRDLAAIAKIPDVDV
VVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLMVTFDDNGVVKAAKGDPILVDSSVKPDETVVA
RVKELAKPIEELRTKVIGKTEAPIDGSRESCRAKECEMGSLVADAMLDRVKGQGVTVAITNGGGLRASIDGGDVTMGEVI
TVLPFQNTLSTFQLKGADIRAALENGLSQVEEGGGRFPQVAGLRFSFDRSKPAGSRLVSVEVEDGGAFAALDPEKTYSLV
SNNFMRGGGDGYAVFKDKGENAYDYGPGLETVLADYLAAHQPFKPYTSGRITEVTAAADAAPGAASEPEAAAAPAESATP
TPAPEPSTESAAAAGPRTHVIARGDTLWDLAQTFYGSGAEWKRISAANGDPAPRALEIGRELEIPAE

Sequences:

>Translated_627_residues
MIRHMRVGFTAVSLFALSSGAAFADYELNILHINDLHSRIESINKFDSTCSVEEEGKNECFGGVARLKTLIDQKRQERTG
KNLLLLNAGDNFQGSLFFTTYKGAAEAEFLNLMKFDAMTVGNHEFDESEDGLASFLDKVTFPVVTANVLPSHKSKIGDRI
KPSIVLDVGGQKVGIVGAVANDTPELASPGPDILIGEDVATITSAVEELKKQGINKIVALTHVGYPRDLAAIAKIPDVDV
VVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLMVTFDDNGVVKAAKGDPILVDSSVKPDETVVA
RVKELAKPIEELRTKVIGKTEAPIDGSRESCRAKECEMGSLVADAMLDRVKGQGVTVAITNGGGLRASIDGGDVTMGEVI
TVLPFQNTLSTFQLKGADIRAALENGLSQVEEGGGRFPQVAGLRFSFDRSKPAGSRLVSVEVEDGGAFAALDPEKTYSLV
SNNFMRGGGDGYAVFKDKGENAYDYGPGLETVLADYLAAHQPFKPYTSGRITEVTAAADAAPGAASEPEAAAAPAESATP
TPAPEPSTESAAAAGPRTHVIARGDTLWDLAQTFYGSGAEWKRISAANGDPAPRALEIGRELEIPAE
>Mature_627_residues
MIRHMRVGFTAVSLFALSSGAAFADYELNILHINDLHSRIESINKFDSTCSVEEEGKNECFGGVARLKTLIDQKRQERTG
KNLLLLNAGDNFQGSLFFTTYKGAAEAEFLNLMKFDAMTVGNHEFDESEDGLASFLDKVTFPVVTANVLPSHKSKIGDRI
KPSIVLDVGGQKVGIVGAVANDTPELASPGPDILIGEDVATITSAVEELKKQGINKIVALTHVGYPRDLAAIAKIPDVDV
VVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLMVTFDDNGVVKAAKGDPILVDSSVKPDETVVA
RVKELAKPIEELRTKVIGKTEAPIDGSRESCRAKECEMGSLVADAMLDRVKGQGVTVAITNGGGLRASIDGGDVTMGEVI
TVLPFQNTLSTFQLKGADIRAALENGLSQVEEGGGRFPQVAGLRFSFDRSKPAGSRLVSVEVEDGGAFAALDPEKTYSLV
SNNFMRGGGDGYAVFKDKGENAYDYGPGLETVLADYLAAHQPFKPYTSGRITEVTAAADAAPGAASEPEAAAAPAESATP
TPAPEPSTESAAAAGPRTHVIARGDTLWDLAQTFYGSGAEWKRISAANGDPAPRALEIGRELEIPAE

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=527, Percent_Identity=37.3814041745731, Blast_Score=329, Evalue=4e-90,
Organism=Escherichia coli, GI1786687, Length=541, Percent_Identity=26.8022181146026, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI1790658, Length=618, Percent_Identity=23.3009708737864, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI19922446, Length=538, Percent_Identity=35.6877323420074, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI24654424, Length=538, Percent_Identity=35.6877323420074, Blast_Score=328, Evalue=6e-90,
Organism=Drosophila melanogaster, GI28573524, Length=523, Percent_Identity=33.8432122370937, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24641187, Length=541, Percent_Identity=34.5656192236599, Blast_Score=306, Evalue=4e-83,
Organism=Drosophila melanogaster, GI19922444, Length=501, Percent_Identity=35.1297405189621, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI221329836, Length=545, Percent_Identity=32.4770642201835, Blast_Score=285, Evalue=9e-77,
Organism=Drosophila melanogaster, GI19921980, Length=479, Percent_Identity=27.348643006263, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI161076508, Length=479, Percent_Identity=27.348643006263, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24652512, Length=479, Percent_Identity=27.348643006263, Blast_Score=157, Evalue=3e-38,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 66246; Mature: 66246

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRHMRVGFTAVSLFALSSGAAFADYELNILHINDLHSRIESINKFDSTCSVEEEGKNEC
CCCEEECCHHHHHHHHHCCCCEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHH
FGGVARLKTLIDQKRQERTGKNLLLLNAGDNFQGSLFFTTYKGAAEAEFLNLMKFDAMTV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEC
GNHEFDESEDGLASFLDKVTFPVVTANVLPSHKSKIGDRIKPSIVLDVGGQKVGIVGAVA
CCCCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCCEEEEEECCCEEEEEEEEC
NDTPELASPGPDILIGEDVATITSAVEELKKQGINKIVALTHVGYPRDLAAIAKIPDVDV
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEE
VVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLMVTFDDNGVVKA
EECCCCCHHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCHHHHCCCEEEEECCCCEEEE
AKGDPILVDSSVKPDETVVARVKELAKPIEELRTKVIGKTEAPIDGSRESCRAKECEMGS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHCCHHH
LVADAMLDRVKGQGVTVAITNGGGLRASIDGGDVTMGEVITVLPFQNTLSTFQLKGADIR
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEHHHEEEEEECCCCCCEEEECCCHHH
AALENGLSQVEEGGGRFPQVAGLRFSFDRSKPAGSRLVSVEVEDGGAFAALDPEKTYSLV
HHHHHHHHHHHHCCCCCCEECCEEEECCCCCCCCCEEEEEEEECCCEEEEECHHHHHHHH
SNNFMRGGGDGYAVFKDKGENAYDYGPGLETVLADYLAAHQPFKPYTSGRITEVTAAADA
HCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
APGAASEPEAAAAPAESATPTPAPEPSTESAAAAGPRTHVIARGDTLWDLAQTFYGSGAE
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEECCCHHHHHHHHHHCCCCC
WKRISAANGDPAPRALEIGRELEIPAE
CEEEECCCCCCCCHHHHCCCCCCCCCC
>Mature Secondary Structure
MIRHMRVGFTAVSLFALSSGAAFADYELNILHINDLHSRIESINKFDSTCSVEEEGKNEC
CCCEEECCHHHHHHHHHCCCCEEEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCCCHHH
FGGVARLKTLIDQKRQERTGKNLLLLNAGDNFQGSLFFTTYKGAAEAEFLNLMKFDAMTV
HHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHCEEEC
GNHEFDESEDGLASFLDKVTFPVVTANVLPSHKSKIGDRIKPSIVLDVGGQKVGIVGAVA
CCCCCCCCCCHHHHHHHHHHHHEEEECCCCCHHHHHCCCCCCEEEEEECCCEEEEEEEEC
NDTPELASPGPDILIGEDVATITSAVEELKKQGINKIVALTHVGYPRDLAAIAKIPDVDV
CCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHCCCCCEE
VVGGHSHSLLSNTDEKAEGPYPTMVDNPGGYKVPVVQAGSYSKYLGDLMVTFDDNGVVKA
EECCCCCHHHCCCCCCCCCCCCCEEECCCCEEEEEEECCCHHHHCCCEEEEECCCCEEEE
AKGDPILVDSSVKPDETVVARVKELAKPIEELRTKVIGKTEAPIDGSRESCRAKECEMGS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCHHHCCHHH
LVADAMLDRVKGQGVTVAITNGGGLRASIDGGDVTMGEVITVLPFQNTLSTFQLKGADIR
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCEEHHHEEEEEECCCCCCEEEECCCHHH
AALENGLSQVEEGGGRFPQVAGLRFSFDRSKPAGSRLVSVEVEDGGAFAALDPEKTYSLV
HHHHHHHHHHHHCCCCCCEECCEEEECCCCCCCCCEEEEEEEECCCEEEEECHHHHHHHH
SNNFMRGGGDGYAVFKDKGENAYDYGPGLETVLADYLAAHQPFKPYTSGRITEVTAAADA
HCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
APGAASEPEAAAAPAESATPTPAPEPSTESAAAAGPRTHVIARGDTLWDLAQTFYGSGAE
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHCCCCCEEEEECCCHHHHHHHHHHCCCCC
WKRISAANGDPAPRALEIGRELEIPAE
CEEEECCCCCCCCHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]