Click here to switch to the map view.

The map label for this gene is clpB [H]

Identifier: 150397697

GI number: 150397697

Start: 2592576

End: 2595182

Strand: Reverse

Name: clpB [H]

Synonym: Smed_2499

Alternate gene names: 150397697

Gene position: 2595182-2592576 (Counterclockwise)

Preceding gene: 150397698

Following gene: 150397695

Centisome position: 68.62

GC content: 62.06

Gene sequence:

>2607_bases
ATGGATATCGAAAAATATTCGGAACGCGTGCGCGGGTTCCTGCAGTCGGCGCAGACCTATGCGCTGGCAGAAGGGCACCA
GCAGTTCACGCCGGAGCACGTCCTCAAGGTCTTGCTCGACGACGACCAGGGCATGGCGGCCTCGCTCATCGAGCGCGCCG
GCGGAAATGCGCGCGAGGCGAAAGTCGGTACCGCCGCAGCTTTGGCCAAGCTTCCGAAGGTGACCGGCGGCAATGGCTCG
GTCTATCTGTCGCAGCCGCTCGCCAAGGTATTCACCGCAGCCGAGGACGCCGCAAAGAAGGCCGGCGACAGCTTCGTCAC
GGTCGAGCGCCTGCTTCTGGCATTGGCGATCGAAAGCTCGGCGGCGACGGCCTCGATTCTCTCCAAGGCCGGCGTTACGC
CGACGAAGCTCAACCAGGTCATCAATGAAATTCGCAAGGGCCGCACCGCCGACAGCGCGAATGCCGAACAGGGCTTCGAC
TCGCTCAAGAAATATGCCCGCGATCTGACCGCCGAGGCGAGGGAAGGCAAGCTCGATCCGGTGATCGGCCGTGACGACGA
GATCCGCCGCACGATCCAGGTGCTTTCGCGCCGAACGAAGAACAATCCTGTTCTGATCGGCGAGCCGGGCGTCGGCAAGA
CCGCGATCGCAGAAGGCCTGGCACTCAGGATCGTCAACGGCGATGTCCCCGAAAGCCTGAAGGACAAGAGGCTGATGGCG
CTCGACATGGGCGCTTTGATCGCCGGTGCAAAGTTCCGCGGTGAGTTCGAAGAGCGGCTCAAGGCCGTGCTGAACGAAGT
GCGCTCGGAGGGGGGAGAGATCATCCTCTTCATCGACGAGATGCATACGCTGGTCGGTGCCGGCAAGGCTGAGGGCGCCA
TGGATGCATCGAACCTCCTGAAGCCCGCGCTTGCCCGCGGCGAGCTGCACTGCGTCGGCGCGACGACTCTCGACGAATAC
CGAAAGCATGTCGAGAAGGACGCAGCCCTTGCCCGGCGGTTTCAGCCGGTGATGGTCGAGGAGCCGACGGTCGAAGATAC
GATCTCGATCCTGCGCGGCTTGAAGGAGAAATACGAGCAGCACCACAAGGTGCGGATCTCCGACTCCGCGCTGGTTGCGG
CTGCCGCACTTTCCAACCGCTACATCACGGATCGTTTCCTTCCGGACAAGGCTATCGACCTCATGGACGAGGCAGCCTCA
CGTCTGAGGATGCAGGTCGACTCCAAACCGGAGGAACTCGACGAGCTGGACCGGCGCGTCATTCAGCTCAAGATCGAACG
CGAAGCCTTGAAGAAGGAGACCGACGTATCCTCCAGGGATCGCCTTTCCAAGCTCGAACTCGACCTGTCCTCGCTCGAAG
AACAGGCGGCGGCATTGACGGCACGCTGGCAGGCGGAGAAGCAGAAGCTCGGCCGGGCGGCAGACCTCAAGAAGCAGCTC
GACGAAGCACGCAACGAACTGCAGATCGCGCAGCGCAAGGGTGAATTCCAGCGGGCAGGCGAACTCGCCTACGGCGTGAT
CCCAAACCTCGAAAAGGAACTTACCGAGGCGGAAAGCCAGGATGGCGCCAGCGCCAACCCGATGGTGCAGGAGGTCGTGA
CCCCAGACAATATTGCTCATATCGTCTCGCGTTGGACCGGCATTCCGGTCGACAAGATGCTGGAAGGCGAGCGCGACAAG
CTGCTCAGGATGGAAGACGAGCTGGCTAAATGGGTCGTCGGTCAGGGCGACGCCGTGCAGGCGGTTTCGCGGGCGGTTCG
CCGTTCGCGCGCAGGGCTACAGGATCCGAACCGGCCGATCGGCTCGTTTATTTTCCTAGGCCCGACGGGCGTCGGCAAGA
CGGAGCTGACCAAGGCGCTTGCCCGGTTCCTCTTCGACGACGAGACCGCGCTTATGCGGATCGACATGTCGGAATATATG
GAGAAGCACTCGGTAGCCCGCCTGATCGGCGCGCCTCCCGGCTATGTCGGCTATGAGGAGGGTGGGGCGCTGACGGAATC
CGTTCGTCGCCGGCCCTATCAGGTCGTGCTCTTCGACGAGATCGAGAAGGCGCATCCGGATGTCTTCAACGTGCTTCTGC
AGGTGCTTGACGATGGACGTCTGACCGATGGTCAGGGGCGCACCGTCGACTTCAAGAACACGATGATCATCATGACCTCG
AACCTCGGTGCGGAATACCTGACCGCACTCGGTGAAAACGAGGACAGCGATGCTGTTCGCGATCAGGTCATGGAGGTGGT
GCGCGCGGCTTTCCGTCCGGAATTCCTGAATCGCGTCGACGAGATCATCCTGTTCCACCGGCTGCGTCGCTCGGAGATGG
GCGCGATCGTCGACATACAGCTTGAAAGGCTGCGCAAGCTCCTGGCGGATCGCAAGATCACGCTCGAGCTTGAGGACGAT
GCACGCGTTTTCCTGGCGGAAAGGGGCTACGATCCTGCTTACGGCGCACGCCCCCTGAAACGGGCGATTCAGAAATATGT
CCAGGATCCGCTGGCCGAGAAGGTACTGCAGGGCGAGTTCCCGGACGGGTCGGTGATCAAAGTCGTTACCGGTTCCGACC
GCCTCAACTTCGAGCGCGGAGCCGGGACGGACAAAGAAGCCGCGTAA

Upstream 100 bases:

>100_bases
GCGGCGACGTCGTACAGGGTTTGCCGAATGCGGGAGCCTGATCCTGAACCGCCGGCCAGTGCCACATTGATGGGCTCGTC
GGGTTATGGAGGTTGAAGAA

Downstream 100 bases:

>100_bases
ACTGCGCATTTCGACATGAGGGCCGCCCTGCAGGGCGGCCCATTTTTGTACGAGCCCGCCATGCCGTTACTGGGCGCCTA
CTTGCTTGCGACTTCGTTCG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 868; Mature: 868

Protein sequence:

>868_residues
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREAKVGTAAALAKLPKVTGGNGS
VYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESSAATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFD
SLKKYARDLTAEAREGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKAEGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQHHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAAS
RLRMQVDSKPEELDELDRRVIQLKIEREALKKETDVSSRDRLSKLELDLSSLEEQAAALTARWQAEKQKLGRAADLKKQL
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELTEAESQDGASANPMVQEVVTPDNIAHIVSRWTGIPVDKMLEGERDK
LLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPIGSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYM
EKHSVARLIGAPPGYVGYEEGGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQLERLRKLLADRKITLELEDD
ARVFLAERGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEFPDGSVIKVVTGSDRLNFERGAGTDKEAA

Sequences:

>Translated_868_residues
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREAKVGTAAALAKLPKVTGGNGS
VYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESSAATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFD
SLKKYARDLTAEAREGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKAEGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQHHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAAS
RLRMQVDSKPEELDELDRRVIQLKIEREALKKETDVSSRDRLSKLELDLSSLEEQAAALTARWQAEKQKLGRAADLKKQL
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELTEAESQDGASANPMVQEVVTPDNIAHIVSRWTGIPVDKMLEGERDK
LLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPIGSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYM
EKHSVARLIGAPPGYVGYEEGGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQLERLRKLLADRKITLELEDD
ARVFLAERGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEFPDGSVIKVVTGSDRLNFERGAGTDKEAA
>Mature_868_residues
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREAKVGTAAALAKLPKVTGGNGS
VYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESSAATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFD
SLKKYARDLTAEAREGKLDPVIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKAEGAMDASNLLKPALARGELHCVGATTLDEY
RKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQHHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAAS
RLRMQVDSKPEELDELDRRVIQLKIEREALKKETDVSSRDRLSKLELDLSSLEEQAAALTARWQAEKQKLGRAADLKKQL
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELTEAESQDGASANPMVQEVVTPDNIAHIVSRWTGIPVDKMLEGERDK
LLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPIGSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYM
EKHSVARLIGAPPGYVGYEEGGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQLERLRKLLADRKITLELEDD
ARVFLAERGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEFPDGSVIKVVTGSDRLNFERGAGTDKEAA

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=427, Percent_Identity=33.4894613583138, Blast_Score=212, Evalue=1e-54,
Organism=Escherichia coli, GI1788943, Length=855, Percent_Identity=58.0116959064327, Blast_Score=1001, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=324, Percent_Identity=44.1358024691358, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6320464, Length=700, Percent_Identity=55.2857142857143, Blast_Score=784, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=863, Percent_Identity=44.3800695249131, Blast_Score=692, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95847; Mature: 95847

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREA
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
KVGTAAALAKLPKVTGGNGSVYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESS
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCH
AATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFDSLKKYARDLTAEAREGKLDP
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCHHHHCHHHH
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKAEGAMDASNLL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQ
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHH
HHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAASRLRMQVDSKPEELDELDRRV
HCCEEECCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IQLKIEREALKKETDVSSRDRLSKLELDLSSLEEQAAALTARWQAEKQKLGRAADLKKQL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELTEAESQDGASANPMVQEVVTPDNIAH
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHH
IVSRWTGIPVDKMLEGERDKLLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPI
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYMEKHSVARLIGAPPGYVGYEE
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
CCHHHHHHHCCCEEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQ
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEHHH
LERLRKLLADRKITLELEDDARVFLAERGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEF
HHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCC
PDGSVIKVVTGSDRLNFERGAGTDKEAA
CCCCEEEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDIEKYSERVRGFLQSAQTYALAEGHQQFTPEHVLKVLLDDDQGMAASLIERAGGNAREA
CCHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHH
KVGTAAALAKLPKVTGGNGSVYLSQPLAKVFTAAEDAAKKAGDSFVTVERLLLALAIESS
HHHHHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCH
AATASILSKAGVTPTKLNQVINEIRKGRTADSANAEQGFDSLKKYARDLTAEAREGKLDP
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
VIGRDDEIRRTIQVLSRRTKNNPVLIGEPGVGKTAIAEGLALRIVNGDVPESLKDKRLMA
CCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCEEEEECCCCCCHHHHCHHHH
LDMGALIAGAKFRGEFEERLKAVLNEVRSEGGEIILFIDEMHTLVGAGKAEGAMDASNLL
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHH
KPALARGELHCVGATTLDEYRKHVEKDAALARRFQPVMVEEPTVEDTISILRGLKEKYEQ
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHH
HHKVRISDSALVAAAALSNRYITDRFLPDKAIDLMDEAASRLRMQVDSKPEELDELDRRV
HCCEEECCHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IQLKIEREALKKETDVSSRDRLSKLELDLSSLEEQAAALTARWQAEKQKLGRAADLKKQL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DEARNELQIAQRKGEFQRAGELAYGVIPNLEKELTEAESQDGASANPMVQEVVTPDNIAH
HHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHH
IVSRWTGIPVDKMLEGERDKLLRMEDELAKWVVGQGDAVQAVSRAVRRSRAGLQDPNRPI
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCC
GSFIFLGPTGVGKTELTKALARFLFDDETALMRIDMSEYMEKHSVARLIGAPPGYVGYEE
CCEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEECHHHHHHHHHHHHHHCCCCCCCCCCC
GGALTESVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDGQGRTVDFKNTMIIMTS
CCHHHHHHHCCCEEEEEEHHHHHHCCHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEEC
NLGAEYLTALGENEDSDAVRDQVMEVVRAAFRPEFLNRVDEIILFHRLRRSEMGAIVDIQ
CCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCEEHHH
LERLRKLLADRKITLELEDDARVFLAERGYDPAYGARPLKRAIQKYVQDPLAEKVLQGEF
HHHHHHHHCCCEEEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCHHHHHHHCCCC
PDGSVIKVVTGSDRLNFERGAGTDKEAA
CCCCEEEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]