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The map label for this gene is fixK [H]

Identifier: 150397600

GI number: 150397600

Start: 2475596

End: 2476348

Strand: Reverse

Name: fixK [H]

Synonym: Smed_2400

Alternate gene names: 150397600

Gene position: 2476348-2475596 (Counterclockwise)

Preceding gene: 150397603

Following gene: 150397599

Centisome position: 65.48

GC content: 59.5

Gene sequence:

>753_bases
ATGATAACCGAAGGAAAGTTTAGCAAAGGCACGCCCTGCAAAGAGTGTCCCTTGCGCCCGCTGCCGCTGTTCCGGTCCTT
CGAGGCCGAAGAAATTGCATTCGTCTCCCGTTTCAAGATCGGCGAACTCGTCTTTGACTCAAATGCGATGATTCTCGCTG
AGGGAGAGGAGAGCGAACACCTCTTTACCGTGCTTTCCGGTTGGGGGTTTCGCTACAAGATGCTGGATGACGGGCGGCGG
CAGATCATGAACTATGTCATGCCCGGCGACATTGTGGGGCTGCAGGGCACCCTGATGGGCGAGATGCAGCATTCGATCGA
GTCGCTTTCGCCCGTGGTGCTCTGCGTTTTTCAGCGCAGCAAGCTCGCCGAGCTTTACAGGTGCCATCCGGAACTGGCCT
ACGACCTGACCTGGATCGCAGCGCGCGAGGAGCGTATGCTGGACGAAAATCTGCTGAGCATCGGCCGCCGATCCGCTCTG
GAAAGGGCCGCCTATCTCATTGCCTTCCTGTACCAGAGGGCTGCGAGCCTTGAACTCTTTCGCAGCGGCTGGAGCGTCAT
TCCCATCACACAGCAACATGTGGCCGACACGCTGGGGCTCTCGCTCGTCCACACCAACAAGACGCTGAAGAAGCTGGCCG
CCAGAGGTCTGCTGCGCTGGGCCGAGAAGGGCTGCGACGTCCATGACATCGACGGCTTGCTTGCGGTTGCGGGCTGGGAC
GGATTGTGCGAACCGAAGAGGCCGCTGATCTGA

Upstream 100 bases:

>100_bases
CGCCTTCACGGCTTCGTCTTCGGCCTTGTCGTTCTTCTGCGCCATGCTACTGTATCGGGTCTGACCATCCGCACCGGTGC
GCCGGGCCTCGAGATACTTC

Downstream 100 bases:

>100_bases
TTGGGCGGCACCATCAAATTTCGTACAACATGTCTTTTTTAAATGCGCCGGAGTTTCCGGGTGTTTACTAGACGACGATG
GTCTTAAGCCCTATTCCTCC

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MITEGKFSKGTPCKECPLRPLPLFRSFEAEEIAFVSRFKIGELVFDSNAMILAEGEESEHLFTVLSGWGFRYKMLDDGRR
QIMNYVMPGDIVGLQGTLMGEMQHSIESLSPVVLCVFQRSKLAELYRCHPELAYDLTWIAAREERMLDENLLSIGRRSAL
ERAAYLIAFLYQRAASLELFRSGWSVIPITQQHVADTLGLSLVHTNKTLKKLAARGLLRWAEKGCDVHDIDGLLAVAGWD
GLCEPKRPLI

Sequences:

>Translated_250_residues
MITEGKFSKGTPCKECPLRPLPLFRSFEAEEIAFVSRFKIGELVFDSNAMILAEGEESEHLFTVLSGWGFRYKMLDDGRR
QIMNYVMPGDIVGLQGTLMGEMQHSIESLSPVVLCVFQRSKLAELYRCHPELAYDLTWIAAREERMLDENLLSIGRRSAL
ERAAYLIAFLYQRAASLELFRSGWSVIPITQQHVADTLGLSLVHTNKTLKKLAARGLLRWAEKGCDVHDIDGLLAVAGWD
GLCEPKRPLI
>Mature_250_residues
MITEGKFSKGTPCKECPLRPLPLFRSFEAEEIAFVSRFKIGELVFDSNAMILAEGEESEHLFTVLSGWGFRYKMLDDGRR
QIMNYVMPGDIVGLQGTLMGEMQHSIESLSPVVLCVFQRSKLAELYRCHPELAYDLTWIAAREERMLDENLLSIGRRSAL
ERAAYLIAFLYQRAASLELFRSGWSVIPITQQHVADTLGLSLVHTNKTLKKLAARGLLRWAEKGCDVHDIDGLLAVAGWD
GLCEPKRPLI

Specific function: B.japonicum has two fixLJ-dependent fixK homologs that are activators of the transcription of a group of genes involved in anaerobic processes such as denitrification and possibly nitrogen fixation. FixK may bind DNA at the FNR consensus binding site [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 28204; Mature: 28204

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITEGKFSKGTPCKECPLRPLPLFRSFEAEEIAFVSRFKIGELVFDSNAMILAEGEESEH
CCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHEEECCCEEEEECCCCCCH
LFTVLSGWGFRYKMLDDGRRQIMNYVMPGDIVGLQGTLMGEMQHSIESLSPVVLCVFQRS
HHHHHHCCCEEEEEHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLAELYRCHPELAYDLTWIAAREERMLDENLLSIGRRSALERAAYLIAFLYQRAASLELF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGWSVIPITQQHVADTLGLSLVHTNKTLKKLAARGLLRWAEKGCDVHDIDGLLAVAGWD
HCCCCEEECCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCC
GLCEPKRPLI
CCCCCCCCCC
>Mature Secondary Structure
MITEGKFSKGTPCKECPLRPLPLFRSFEAEEIAFVSRFKIGELVFDSNAMILAEGEESEH
CCCCCCCCCCCCHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHEEECCCEEEEECCCCCCH
LFTVLSGWGFRYKMLDDGRRQIMNYVMPGDIVGLQGTLMGEMQHSIESLSPVVLCVFQRS
HHHHHHCCCEEEEEHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
KLAELYRCHPELAYDLTWIAAREERMLDENLLSIGRRSALERAAYLIAFLYQRAASLELF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGWSVIPITQQHVADTLGLSLVHTNKTLKKLAARGLLRWAEKGCDVHDIDGLLAVAGWD
HCCCCEEECCHHHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHCCCEECCCCEEEEECCC
GLCEPKRPLI
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1551834; 12597275 [H]