BacMap

Definition	Sinorhizobium medicae WSM419 chromosome, complete genome.
Accession	NC_009636
Length	3,781,904

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The map label for this gene is braF [H]

Identifier: 150397595

GI number: 150397595

Start: 2470532

End: 2471419

Strand: Reverse

Name: braF [H]

Synonym: Smed_2395

Alternate gene names: 150397595

Gene position: 2471419-2470532 (Counterclockwise)

Preceding gene: 150397596

Following gene: 150397594

Centisome position: 65.35

GC content: 59.46

Gene sequence:

>888_bases
ATGGCGATCGGGACAGAGGCAATGAAAAAAGACCCCATCCTGAAGGTTGAGCGTCTGTCGATGCGCTTCGGCGGTCTGAT
GGCCATCAACGACTTCTCCTTCGAGGCGGAACGCGGCGAGATCACGGCGCTGATCGGCCCGAACGGAGCAGGCAAGACAA
CGGTGTTCAACTGCATCACCGGCTTCTACAAGCCGACGATGGGCATGATCACCCTGCGGCAGAATTCCGGCGACGAGTTC
CTTCTGGAGCGGCTGCCGGATTTCGAAATCACCAAGAAGGCGAAGGTGGCACGCACCTTCCAGAACATCCGCATGTTCTC
GGGCCTGACGGTTCTGGAAAACCTGCTCGTCGCGCAACATAACAAGCTGATGCGGGCATCCGGCTATACCATTCTCGGCC
TTTTGGGCTTCCCGACCTATCGCGAGGCGTCAAGGGAATCAATCGAACTCGCCAGGCACTGGTTGGAAAAAGCTTCGCTG
ATCGAGCGGGCCGACGATCCGGCCGGCGACCTTCCCTATGGTGCTCAGCGGCGGCTGGAGATCGCCCGCGCAATGTGCAC
CGGACCGGAGCTTCTCTGCCTGGACGAGCCCGCCGCGGGCCTCAACCCGCGCGAGTCGCTCGCGCTGAATGAACTGCTGC
AGGAGATTCGCCGCGATACCGGCACCTCCATCCTGTTGATCGAGCACGACATGTCCGTGGTGATGGAGATATCCGATCAC
GTCGTGGTTCTCGAATACGGTCAGAAGATTTCCGACGGCACCCCGGATTTCGTGAAGAACGATCCAAAGGTCATTGCGGC
CTATCTGGGCGTCGAGGATGAAGAGGTTGACGAGGTGATTGGAGAGATCGAGGAAATCGAAGGCGAGCATGGGGGGACCA
GGCAATGA

Upstream 100 bases:

>100_bases
TCTGGAAGCCGCGTGGCTTCGTCGGATCACGAGAGCCGACCGCGTTCCTGCGCCAACGCAGGGCAGTCTCCGGCAGCTTC
ACCAAAGAGGGGCACGGCTG

Downstream 100 bases:

>100_bases
CGGCGCCCCTGCTGCATGTGAGAGGCGTCGAGACCTATTACGGCAATATCCGGGCGCTGAACGGCGTCGACATGGAGGTC
CATCGCGGCGAAATCGTTGC

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MAIGTEAMKKDPILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCITGFYKPTMGMITLRQNSGDEF
LLERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQHNKLMRASGYTILGLLGFPTYREASRESIELARHWLEKASL
IERADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESLALNELLQEIRRDTGTSILLIEHDMSVVMEISDH
VVVLEYGQKISDGTPDFVKNDPKVIAAYLGVEDEEVDEVIGEIEEIEGEHGGTRQ

Sequences:

>Translated_295_residues
MAIGTEAMKKDPILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCITGFYKPTMGMITLRQNSGDEF
LLERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQHNKLMRASGYTILGLLGFPTYREASRESIELARHWLEKASL
IERADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESLALNELLQEIRRDTGTSILLIEHDMSVVMEISDH
VVVLEYGQKISDGTPDFVKNDPKVIAAYLGVEDEEVDEVIGEIEEIEGEHGGTRQ
>Mature_294_residues
AIGTEAMKKDPILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCITGFYKPTMGMITLRQNSGDEFL
LERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQHNKLMRASGYTILGLLGFPTYREASRESIELARHWLEKASLI
ERADDPAGDLPYGAQRRLEIARAMCTGPELLCLDEPAAGLNPRESLALNELLQEIRRDTGTSILLIEHDMSVVMEISDHV
VVLEYGQKISDGTPDFVKNDPKVIAAYLGVEDEEVDEVIGEIEEIEGEHGGTRQ

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI21536376, Length=248, Percent_Identity=26.6129032258064, Blast_Score=78, Evalue=9e-15,
Organism=Homo sapiens, GI27881501, Length=237, Percent_Identity=25.3164556962025, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI105990541, Length=238, Percent_Identity=27.3109243697479, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI30795238, Length=237, Percent_Identity=25.3164556962025, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI31657092, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI27477115, Length=219, Percent_Identity=31.9634703196347, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI9665248, Length=234, Percent_Identity=29.4871794871795, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI153792144, Length=234, Percent_Identity=26.9230769230769, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI47078218, Length=233, Percent_Identity=27.4678111587983, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI45446740, Length=233, Percent_Identity=27.4678111587983, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI116734710, Length=239, Percent_Identity=26.7782426778243, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI46592971, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592915, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592956, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI46592898, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI46592978, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI46592964, Length=216, Percent_Identity=25.462962962963, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI27436953, Length=238, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789864, Length=261, Percent_Identity=52.8735632183908, Blast_Score=268, Evalue=4e-73,
Organism=Escherichia coli, GI1786398, Length=239, Percent_Identity=31.7991631799163, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI87082268, Length=262, Percent_Identity=29.7709923664122, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787029, Length=253, Percent_Identity=27.2727272727273, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1789672, Length=261, Percent_Identity=25.2873563218391, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788225, Length=260, Percent_Identity=27.3076923076923, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1790525, Length=254, Percent_Identity=27.5590551181102, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1788761, Length=275, Percent_Identity=26.9090909090909, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1789593, Length=258, Percent_Identity=26.7441860465116, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788210, Length=246, Percent_Identity=28.0487804878049, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1788165, Length=256, Percent_Identity=26.953125, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI48995001, Length=235, Percent_Identity=28.0851063829787, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789032, Length=251, Percent_Identity=25.4980079681275, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789991, Length=249, Percent_Identity=29.3172690763052, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788450, Length=262, Percent_Identity=26.7175572519084, Blast_Score=91, Evalue=9e-20,
Organism=Escherichia coli, GI87081782, Length=265, Percent_Identity=28.3018867924528, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081709, Length=265, Percent_Identity=24.1509433962264, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787759, Length=268, Percent_Identity=28.3582089552239, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1790544, Length=257, Percent_Identity=26.0700389105058, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1788644, Length=263, Percent_Identity=27.7566539923954, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1786872, Length=253, Percent_Identity=25.6916996047431, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI48994997, Length=255, Percent_Identity=27.0588235294118, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787370, Length=261, Percent_Identity=27.2030651340996, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787089, Length=233, Percent_Identity=25.3218884120172, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI87081791, Length=252, Percent_Identity=25, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI48994943, Length=238, Percent_Identity=27.7310924369748, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790190, Length=247, Percent_Identity=25.5060728744939, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790162, Length=268, Percent_Identity=26.865671641791, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787712, Length=264, Percent_Identity=25, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1786803, Length=246, Percent_Identity=24.7967479674797, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788897, Length=256, Percent_Identity=26.171875, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789962, Length=243, Percent_Identity=27.9835390946502, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787500, Length=243, Percent_Identity=28.3950617283951, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87082267, Length=260, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789873, Length=238, Percent_Identity=26.890756302521, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081834, Length=210, Percent_Identity=26.1904761904762, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790467, Length=259, Percent_Identity=22.007722007722, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786345, Length=250, Percent_Identity=26.4, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1788472, Length=262, Percent_Identity=24.0458015267176, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786975, Length=206, Percent_Identity=27.1844660194175, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789963, Length=266, Percent_Identity=25.5639097744361, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI48994883, Length=261, Percent_Identity=25.2873563218391, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693107, Length=260, Percent_Identity=23.8461538461538, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786703, Length=250, Percent_Identity=26, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1786253, Length=240, Percent_Identity=26.25, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787164, Length=255, Percent_Identity=24.7058823529412, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787143, Length=237, Percent_Identity=28.2700421940928, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787105, Length=268, Percent_Identity=24.6268656716418, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=233, Percent_Identity=27.4678111587983, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71983523, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71983516, Length=234, Percent_Identity=28.2051282051282, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=234, Percent_Identity=29.4871794871795, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI115533608, Length=222, Percent_Identity=31.0810810810811, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666092, Length=262, Percent_Identity=28.2442748091603, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24648314, Length=255, Percent_Identity=25.4901960784314, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24643674, Length=263, Percent_Identity=26.2357414448669, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28573571, Length=271, Percent_Identity=24.3542435424354, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI28573573, Length=261, Percent_Identity=24.904214559387, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19920532, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24580930, Length=250, Percent_Identity=26, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161076682, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076684, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581617, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24581615, Length=234, Percent_Identity=26.0683760683761, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI116007184, Length=239, Percent_Identity=24.6861924686192, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI221500365, Length=239, Percent_Identity=24.6861924686192, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24581191, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581189, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581193, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581195, Length=220, Percent_Identity=26.3636363636364, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574744, Length=217, Percent_Identity=27.1889400921659, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581387, Length=225, Percent_Identity=25.3333333333333, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 32731; Mature: 32600

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGTEAMKKDPILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCIT
CCCCCHHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHH
GFYKPTMGMITLRQNSGDEFLLERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQH
HHHCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLMRASGYTILGLLGFPTYREASRESIELARHWLEKASLIERADDPAGDLPYGAQRRLE
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IARAMCTGPELLCLDEPAAGLNPRESLALNELLQEIRRDTGTSILLIEHDMSVVMEISDH
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
VVVLEYGQKISDGTPDFVKNDPKVIAAYLGVEDEEVDEVIGEIEEIEGEHGGTRQ
EEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AIGTEAMKKDPILKVERLSMRFGGLMAINDFSFEAERGEITALIGPNGAGKTTVFNCIT
CCCCHHHHCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCCHHHHHHHH
GFYKPTMGMITLRQNSGDEFLLERLPDFEITKKAKVARTFQNIRMFSGLTVLENLLVAQH
HHHCCCCCEEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKLMRASGYTILGLLGFPTYREASRESIELARHWLEKASLIERADDPAGDLPYGAQRRLE
HHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
IARAMCTGPELLCLDEPAAGLNPRESLALNELLQEIRRDTGTSILLIEHDMSVVMEISDH
HHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCC
VVVLEYGQKISDGTPDFVKNDPKVIAAYLGVEDEEVDEVIGEIEEIEGEHGGTRQ
EEEEECCCCCCCCCCHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]