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The map label for this gene is braE [H]

Identifier: 150397596

GI number: 150397596

Start: 2471419

End: 2472804

Strand: Reverse

Name: braE [H]

Synonym: Smed_2396

Alternate gene names: 150397596

Gene position: 2472804-2471419 (Counterclockwise)

Preceding gene: 150397597

Following gene: 150397595

Centisome position: 65.39

GC content: 60.82

Gene sequence:

>1386_bases
ATGGCAAATGTCGCATCCACCACCGCAAGCGCCGGCAGCGAACTGACGGCGCGGGCGCTTCGCGAGGCTGTTTTTGCGGG
CCTCATCACGCTTGGACTGTTCGTGCTCTTCGTCGGGCTCAAGTCCGACCAGAACATCCGCAACGAACTCATTCTCACCC
AGCGCTGGGGACTGCTTGCGATATTCGTGGCGGTTGCCATGGTTGGCCGTTTCCTCATCGTTGTCTATGTGCAGCCGCGG
TTCGCCAAACGGAAGGCGGCAAAGGCGGCCGCGCCCGACGTGGTGAAGAAGGACACCTTTTTCACCCGCCAATGGCCGAA
GATCGCGGTCGTCATGCTGCTGCTTTACCCCCCGGTCATCGTCTCGTTCGTGGGCGTCCAGGGGTCGCTGAAATGGGTCG
ACAATTTCGGCATTCAGATCCTGATCTACGTGATGCTTGCCTGGGGCCTCAACATCGTCGTCGGTCTCGCCGGGTTGCTC
GATCTCGGCTATGTGGCCTTCTATGCCGTGGGCGCCTATTCCTACGCGCTTCTCTCCAGTTATTTCGGACTTTCCTTCTG
GGTGCTGCTGCCTGTCGCCGGACTTCTCGCCGCCCTCTGGGGCATCGTTCTCGGCTTCCCGGTGCTGCGCCTCAGGGGTG
ACTATCTGGCGATCGTCACGCTCGCTTTCGGAGAGATCATCCGCCTCGTGCTGATCAACTGGACCGAGGTTACCAAGGGG
ACATTCGGCGTCTCCGGCATCGCCAAGGCGACGCTTTTCGGCATCAAGTTCGATGCTTCGAAGGATGGTTTTGCGGCGCT
CTTCGACTTGCCTCTCTCTTCGGCCTATTACAAGATCTTCCTGTTCTATCTGATCCTCGCGCTCGCCCTTCTGACCGCCT
TCGTAACGATCCGGCTGCGCCGAATGCCGATCGGGCGCGCATGGGAGGCGTTGCGCGAAGACGAGATCGCCTGCCGTTCG
CTCGGCATCAACACTGTCACCACCAAGCTGACGGCCTTTGCGACGGGTGCGATGTTCGGCGGGTTTGCAGGCTCCTTTTT
TGCCGTGCGTCAGGGCTTCGTCTCGCCGGAATCCTTCGTCTTCCTGGAATCGGCCGTCATCCTTGCCATCGTCGTTCTTG
GCGGCATGGGCTCGCTGACCGGTATCGCCATTGCCGCCGTCGTTATGATCGGCGGCACCGAAATCCTGCGTGAGCTGACG
TTCCTCAAGATGATCTTCGGACCGGCCTTCACACCCGAACTCTATCGCATGCTGATCTTCGGCCTGGCCATGGTGACGGT
CATGGTCTGGAAGCCGCGTGGCTTCGTCGGATCACGAGAGCCGACCGCGTTCCTGCGCCAACGCAGGGCAGTCTCCGGCA
GCTTCACCAAAGAGGGGCACGGCTGA

Upstream 100 bases:

>100_bases
GACTACAAGGACGTCGCAACCTTTTCCATTCTCGCGATCGTCCTGATCTTCAAGCCGTCGGGTATTCTCGGACGACCGGA
AGTCGAGAAGGTATAGTTTC

Downstream 100 bases:

>100_bases
TGGCGATCGGGACAGAGGCAATGAAAAAAGACCCCATCCTGAAGGTTGAGCGTCTGTCGATGCGCTTCGGCGGTCTGATG
GCCATCAACGACTTCTCCTT

Product: inner-membrane translocator

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MANVASTTASAGSELTARALREAVFAGLITLGLFVLFVGLKSDQNIRNELILTQRWGLLAIFVAVAMVGRFLIVVYVQPR
FAKRKAAKAAAPDVVKKDTFFTRQWPKIAVVMLLLYPPVIVSFVGVQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLL
DLGYVAFYAVGAYSYALLSSYFGLSFWVLLPVAGLLAALWGIVLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTEVTKG
TFGVSGIAKATLFGIKFDASKDGFAALFDLPLSSAYYKIFLFYLILALALLTAFVTIRLRRMPIGRAWEALREDEIACRS
LGINTVTTKLTAFATGAMFGGFAGSFFAVRQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAVVMIGGTEILRELT
FLKMIFGPAFTPELYRMLIFGLAMVTVMVWKPRGFVGSREPTAFLRQRRAVSGSFTKEGHG

Sequences:

>Translated_461_residues
MANVASTTASAGSELTARALREAVFAGLITLGLFVLFVGLKSDQNIRNELILTQRWGLLAIFVAVAMVGRFLIVVYVQPR
FAKRKAAKAAAPDVVKKDTFFTRQWPKIAVVMLLLYPPVIVSFVGVQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLL
DLGYVAFYAVGAYSYALLSSYFGLSFWVLLPVAGLLAALWGIVLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTEVTKG
TFGVSGIAKATLFGIKFDASKDGFAALFDLPLSSAYYKIFLFYLILALALLTAFVTIRLRRMPIGRAWEALREDEIACRS
LGINTVTTKLTAFATGAMFGGFAGSFFAVRQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAVVMIGGTEILRELT
FLKMIFGPAFTPELYRMLIFGLAMVTVMVWKPRGFVGSREPTAFLRQRRAVSGSFTKEGHG
>Mature_460_residues
ANVASTTASAGSELTARALREAVFAGLITLGLFVLFVGLKSDQNIRNELILTQRWGLLAIFVAVAMVGRFLIVVYVQPRF
AKRKAAKAAAPDVVKKDTFFTRQWPKIAVVMLLLYPPVIVSFVGVQGSLKWVDNFGIQILIYVMLAWGLNIVVGLAGLLD
LGYVAFYAVGAYSYALLSSYFGLSFWVLLPVAGLLAALWGIVLGFPVLRLRGDYLAIVTLAFGEIIRLVLINWTEVTKGT
FGVSGIAKATLFGIKFDASKDGFAALFDLPLSSAYYKIFLFYLILALALLTAFVTIRLRRMPIGRAWEALREDEIACRSL
GINTVTTKLTAFATGAMFGGFAGSFFAVRQGFVSPESFVFLESAVILAIVVLGGMGSLTGIAIAAVVMIGGTEILRELTF
LKMIFGPAFTPELYRMLIFGLAMVTVMVWKPRGFVGSREPTAFLRQRRAVSGSFTKEGHG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=312, Percent_Identity=52.8846153846154, Blast_Score=295, Evalue=4e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 50095; Mature: 49964

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANVASTTASAGSELTARALREAVFAGLITLGLFVLFVGLKSDQNIRNELILTQRWGLLA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHHHHHH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HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]