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The map label for this gene is rutR [H]

Identifier: 150397565

GI number: 150397565

Start: 2443156

End: 2443797

Strand: Reverse

Name: rutR [H]

Synonym: Smed_2365

Alternate gene names: 150397565

Gene position: 2443797-2443156 (Counterclockwise)

Preceding gene: 150397568

Following gene: 150397562

Centisome position: 64.62

GC content: 59.66

Gene sequence:

>642_bases
ATGGTACTTCCGAGAGCGGCTAGAACCCAGAGGCGCACGCGAATCCAGGAGGAGAAGGAGGAGCAGATTCTCGAGGCGGC
GCTGGAGGTGTTTTCCGCAAACGGCTTTCGCGGTGCGACCGTCGATCAGATCGCCGAGGTGGCGGGCATGTCGAAGCCGA
ATCTGCTCTATTATTTCCGCACCAAAGAGGCGATGCACCGGTCGCTGATCGACCGCGTCCTCGACACCTGGCTTGACCCT
TTGCGCGCGTTCGATGCGGAAGGCAATCCGGTAGCCGAAATACGCAGTTATATCAGGCGAAAACTTGAGATGGCGCGGGA
CTTTCCGCGCGAAAGCCGGTTGTTCGCCAATGAGGTCCTGCAGGGCGCTCCGCATATCGTGGACGAGCTCAAGGGACCAC
TCAAGCAACTTGTGGACGAGAAGGCCGAAGTCATCCGCAGTTGGGCGAAGGCCGGCAAGATCGCGAAGTGCGATCCTTAT
CACCTGATCTTCGCTATTTGGTCGACGACCCAGCATTACGCCGATTTCGACGTTCAGGTGCGCGCCGTGCTGGGCCAGGA
AAATGACGGCGAGGGGCGCTTTGAGGATGCGGCGCGCTTTCTGGAGGAACTGTTCGTCGGTGGGTTGCAGGTGCGTGAGT
AG

Upstream 100 bases:

>100_bases
AAAATAAAAAACAGGAGTTGTTGCTTAAAAAACCGGCGGGTCCGTATTTTGGGCAAATTGCGCCGGACGTCATGCGTCGG
GGGTGTGGACAGGGGACGAA

Downstream 100 bases:

>100_bases
CTTCGCTCGGGGATTCGCCCCTCATCCGGCTGCCACCGCCTTCTTCTTGTTTTTCGACGGGGAGAAGGGACTCGCGGACG
GCCTTCATCTCTGTAGAGGG

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MVLPRAARTQRRTRIQEEKEEQILEAALEVFSANGFRGATVDQIAEVAGMSKPNLLYYFRTKEAMHRSLIDRVLDTWLDP
LRAFDAEGNPVAEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIVDELKGPLKQLVDEKAEVIRSWAKAGKIAKCDPY
HLIFAIWSTTQHYADFDVQVRAVLGQENDGEGRFEDAARFLEELFVGGLQVRE

Sequences:

>Translated_213_residues
MVLPRAARTQRRTRIQEEKEEQILEAALEVFSANGFRGATVDQIAEVAGMSKPNLLYYFRTKEAMHRSLIDRVLDTWLDP
LRAFDAEGNPVAEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIVDELKGPLKQLVDEKAEVIRSWAKAGKIAKCDPY
HLIFAIWSTTQHYADFDVQVRAVLGQENDGEGRFEDAARFLEELFVGGLQVRE
>Mature_213_residues
MVLPRAARTQRRTRIQEEKEEQILEAALEVFSANGFRGATVDQIAEVAGMSKPNLLYYFRTKEAMHRSLIDRVLDTWLDP
LRAFDAEGNPVAEIRSYIRRKLEMARDFPRESRLFANEVLQGAPHIVDELKGPLKQLVDEKAEVIRSWAKAGKIAKCDPY
HLIFAIWSTTQHYADFDVQVRAVLGQENDGEGRFEDAARFLEELFVGGLQVRE

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=204, Percent_Identity=49.0196078431373, Blast_Score=208, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24418; Mature: 24418

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLPRAARTQRRTRIQEEKEEQILEAALEVFSANGFRGATVDQIAEVAGMSKPNLLYYFR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEC
TKEAMHRSLIDRVLDTWLDPLRAFDAEGNPVAEIRSYIRRKLEMARDFPRESRLFANEVL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QGAPHIVDELKGPLKQLVDEKAEVIRSWAKAGKIAKCDPYHLIFAIWSTTQHYADFDVQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
RAVLGQENDGEGRFEDAARFLEELFVGGLQVRE
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MVLPRAARTQRRTRIQEEKEEQILEAALEVFSANGFRGATVDQIAEVAGMSKPNLLYYFR
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEC
TKEAMHRSLIDRVLDTWLDPLRAFDAEGNPVAEIRSYIRRKLEMARDFPRESRLFANEVL
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
QGAPHIVDELKGPLKQLVDEKAEVIRSWAKAGKIAKCDPYHLIFAIWSTTQHYADFDVQV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHH
RAVLGQENDGEGRFEDAARFLEELFVGGLQVRE
HHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]