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The map label for this gene is gltD [H]

Identifier: 150397560

GI number: 150397560

Start: 2436352

End: 2437713

Strand: Direct

Name: gltD [H]

Synonym: Smed_2360

Alternate gene names: 150397560

Gene position: 2436352-2437713 (Clockwise)

Preceding gene: 150397550

Following gene: 150397561

Centisome position: 64.42

GC content: 64.17

Gene sequence:

>1362_bases
ATGGGAACGACTCAATCTGGGATTCTCGGCGGGCGCTTGCCGCTTGCCGAATACGAAGCCAATTTTTCCGACCTTCACGC
GCCTCTCGACAGGCATGAGGCGCTGGTCGCCGCGGATCGCTGTTACTTCTGTCATGACGCGCCCTGCATGACGGCCTGTC
CCACCGCCATAGACATCCCCCTCTTCATCCGGCAGATCGCGACCGGTAATCCCATCGGCTCGGCCAAGACGATCTTCGAT
CAGAACATACTTGGTGGAATGTGCGCCCGCGTCTGTCCCACCGAAACGCTCTGCGAAGAAGCCTGCGTGCGCAACACGGC
AGAGGAGCGGCCGGTCGAAATCGGCCGTCTGCAGCGCTATGCTTCCGACATCGCGATGACGGAAAACAAACAGTTCTACA
CGCGATCCGCCCGCACCGGCCGCCGGGTCGCGGTCGTTGGCGCCGGACCCGCGGGGCTCGCCTGCGCGCACCGGCTGGCC
GTGAAGGGCCATGACGTGGTGATCTACGATGCGCGCGAAAAGGCCGGTGGCCTGAACGAATACGGCATTGCCGCCTATAA
GGCGGTCGACGATTTCGCCCAGAAGGAAGTCGACTATGTGCTTTCGATCGGCGGCATCGAGGTCAGTCACGGCAAAGCGC
TCGGCCGCGACTTCTCTCTTGCCGACCTGGCAGGCGAATTCGACGCTGTCTTTCTCGGCCTCGGCCTCGCAGGGGTAAAC
GCACTTCGCATTGAAGGCGAGAACGCCGAAGGCGTAGAGGATGCCGTCGATTTCATCGCAGCACTTCGCCAGTCGAAGAC
AAAGGCCGACATTCCGGTCGGCCGGCGCGTCGTGGTGCTCGGCGGCGGCATGACTGCGATCGATGCCGCCGTGCAGGCAA
AGCTGCTCGGCGCAGAGGAGGTGACGATCTGCTATCGCCGCGGCAAGGAGCATATGAACGCCTCCGGATTCGAGCAGGAC
CTCGCGGCGTCCAAGGGCGTCACCATCCGTCACTGGCTGCAGCCGAAGCGTATTGCGGTCAAGGACGGCAGGGTCGCCGG
CATCGAACTCGACTACACCACGCTCGATAACGGGAAGCTGACGACGACCGGGGAGACCGGCATCCTCGCCGCCGATCAGA
TCTTCAAGGCCATCGGTCAGACCTTCGAGGCCTCCGCCCTCGGCGCGCTTCGGATGAAAAGCGGCCGCATCGTCGTCGAT
GCCGAAGGACGCACGTCGCTGGAGAAGGTCTGGGCCGGCGGCGACTGCGTGCTCGGCGGCGAGGATCTCACCGTTTCCGC
CGTTGCGATGGGACGCGATGCGGCCGGATCGATCGACCGGGCTTTCGCCGCGGCTCAACCGCTGGCGAGTGCCGTTGCCT
GA

Upstream 100 bases:

>100_bases
TCCCGGGCTTTGCGCCCACCGGGAAGAAACTTTTACCGTTTGATAAAAAAATAAAGCGTGTTACCGTTCCTTCATCCTGA
GAAAACATTGGGAGCCAAAA

Downstream 100 bases:

>100_bases
GCGCGTAAATGGATCGAGAGGACGAGAACAATGGCTGATCTTCGCAACAATTTCGTCGGCATCAAATCCCCCAACCCATT
CTGGCTCGCCTCGGCGCCGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MGTTQSGILGGRLPLAEYEANFSDLHAPLDRHEALVAADRCYFCHDAPCMTACPTAIDIPLFIRQIATGNPIGSAKTIFD
QNILGGMCARVCPTETLCEEACVRNTAEERPVEIGRLQRYASDIAMTENKQFYTRSARTGRRVAVVGAGPAGLACAHRLA
VKGHDVVIYDAREKAGGLNEYGIAAYKAVDDFAQKEVDYVLSIGGIEVSHGKALGRDFSLADLAGEFDAVFLGLGLAGVN
ALRIEGENAEGVEDAVDFIAALRQSKTKADIPVGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASGFEQD
LAASKGVTIRHWLQPKRIAVKDGRVAGIELDYTTLDNGKLTTTGETGILAADQIFKAIGQTFEASALGALRMKSGRIVVD
AEGRTSLEKVWAGGDCVLGGEDLTVSAVAMGRDAAGSIDRAFAAAQPLASAVA

Sequences:

>Translated_453_residues
MGTTQSGILGGRLPLAEYEANFSDLHAPLDRHEALVAADRCYFCHDAPCMTACPTAIDIPLFIRQIATGNPIGSAKTIFD
QNILGGMCARVCPTETLCEEACVRNTAEERPVEIGRLQRYASDIAMTENKQFYTRSARTGRRVAVVGAGPAGLACAHRLA
VKGHDVVIYDAREKAGGLNEYGIAAYKAVDDFAQKEVDYVLSIGGIEVSHGKALGRDFSLADLAGEFDAVFLGLGLAGVN
ALRIEGENAEGVEDAVDFIAALRQSKTKADIPVGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASGFEQD
LAASKGVTIRHWLQPKRIAVKDGRVAGIELDYTTLDNGKLTTTGETGILAADQIFKAIGQTFEASALGALRMKSGRIVVD
AEGRTSLEKVWAGGDCVLGGEDLTVSAVAMGRDAAGSIDRAFAAAQPLASAVA
>Mature_452_residues
GTTQSGILGGRLPLAEYEANFSDLHAPLDRHEALVAADRCYFCHDAPCMTACPTAIDIPLFIRQIATGNPIGSAKTIFDQ
NILGGMCARVCPTETLCEEACVRNTAEERPVEIGRLQRYASDIAMTENKQFYTRSARTGRRVAVVGAGPAGLACAHRLAV
KGHDVVIYDAREKAGGLNEYGIAAYKAVDDFAQKEVDYVLSIGGIEVSHGKALGRDFSLADLAGEFDAVFLGLGLAGVNA
LRIEGENAEGVEDAVDFIAALRQSKTKADIPVGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASGFEQDL
AASKGVTIRHWLQPKRIAVKDGRVAGIELDYTTLDNGKLTTTGETGILAADQIFKAIGQTFEASALGALRMKSGRIVVDA
EGRTSLEKVWAGGDCVLGGEDLTVSAVAMGRDAAGSIDRAFAAAQPLASAVA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=454, Percent_Identity=32.8193832599119, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI237757300, Length=108, Percent_Identity=46.2962962962963, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1789606, Length=452, Percent_Identity=34.070796460177, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=33.9805825242718, Blast_Score=197, Evalue=9e-52,
Organism=Escherichia coli, GI87082180, Length=454, Percent_Identity=33.920704845815, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788811, Length=456, Percent_Identity=32.6754385964912, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789243, Length=405, Percent_Identity=30.3703703703704, Blast_Score=128, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI71984108, Length=455, Percent_Identity=34.7252747252747, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17570289, Length=454, Percent_Identity=25.5506607929515, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6320030, Length=281, Percent_Identity=30.9608540925267, Blast_Score=114, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24640763, Length=455, Percent_Identity=33.6263736263736, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI18858217, Length=455, Percent_Identity=33.6263736263736, Blast_Score=197, Evalue=1e-50,
Organism=Drosophila melanogaster, GI24665547, Length=468, Percent_Identity=26.4957264957265, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24665543, Length=468, Percent_Identity=26.4957264957265, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI28574881, Length=447, Percent_Identity=27.2930648769575, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24665539, Length=447, Percent_Identity=27.2930648769575, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 47789; Mature: 47658

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTQSGILGGRLPLAEYEANFSDLHAPLDRHEALVAADRCYFCHDAPCMTACPTAIDIP
CCCCCCCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCHHCCCCCCCHH
LFIRQIATGNPIGSAKTIFDQNILGGMCARVCPTETLCEEACVRNTAEERPVEIGRLQRY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHHH
ASDIAMTENKQFYTRSARTGRRVAVVGAGPAGLACAHRLAVKGHDVVIYDAREKAGGLNE
HHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHEECCCEEEEEECHHHCCCCCC
YGIAAYKAVDDFAQKEVDYVLSIGGIEVSHGKALGRDFSLADLAGEFDAVFLGLGLAGVN
CCHHHHHHHHHHHHHHHHEEEEECCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHCCE
ALRIEGENAEGVEDAVDFIAALRQSKTKADIPVGRRVVVLGGGMTAIDAAVQAKLLGAEE
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCH
VTICYRRGKEHMNASGFEQDLAASKGVTIRHWLQPKRIAVKDGRVAGIELDYTTLDNGKL
HHHHHHCCHHHCCCCCCHHHHHHCCCCEEEECCCCCEEEEECCEEEEEEEEEEECCCCEE
TTTGETGILAADQIFKAIGQTFEASALGALRMKSGRIVVDAEGRTSLEKVWAGGDCVLGG
EECCCCCEEEHHHHHHHHCCHHHHHHHHHEEECCCCEEEECCCCCCHHHHHCCCCEEECC
EDLTVSAVAMGRDAAGSIDRAFAAAQPLASAVA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GTTQSGILGGRLPLAEYEANFSDLHAPLDRHEALVAADRCYFCHDAPCMTACPTAIDIP
CCCCCCCCCCCCCHHHHCCCHHHHCCCHHHHHHHHHHCCEEEECCCCCHHCCCCCCCHH
LFIRQIATGNPIGSAKTIFDQNILGGMCARVCPTETLCEEACVRNTAEERPVEIGRLQRY
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHCCHHHHHHHHH
ASDIAMTENKQFYTRSARTGRRVAVVGAGPAGLACAHRLAVKGHDVVIYDAREKAGGLNE
HHHHHCCCCCCEEECCCCCCCEEEEEECCCHHHHHHHHHEECCCEEEEEECHHHCCCCCC
YGIAAYKAVDDFAQKEVDYVLSIGGIEVSHGKALGRDFSLADLAGEFDAVFLGLGLAGVN
CCHHHHHHHHHHHHHHHHEEEEECCEEEECCCCCCCCCCHHHHCCCHHHHHHHHHHHCCE
ALRIEGENAEGVEDAVDFIAALRQSKTKADIPVGRRVVVLGGGMTAIDAAVQAKLLGAEE
EEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHCCCCH
VTICYRRGKEHMNASGFEQDLAASKGVTIRHWLQPKRIAVKDGRVAGIELDYTTLDNGKL
HHHHHHCCHHHCCCCCCHHHHHHCCCCEEEECCCCCEEEEECCEEEEEEEEEEECCCCEE
TTTGETGILAADQIFKAIGQTFEASALGALRMKSGRIVVDAEGRTSLEKVWAGGDCVLGG
EECCCCCEEEHHHHHHHHCCHHHHHHHHHEEECCCCEEEECCCCCCHHHHHCCCCEEECC
EDLTVSAVAMGRDAAGSIDRAFAAAQPLASAVA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]