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The map label for this gene is aglA [H]

Identifier: 150397520

GI number: 150397520

Start: 2400629

End: 2402272

Strand: Reverse

Name: aglA [H]

Synonym: Smed_2320

Alternate gene names: 150397520

Gene position: 2402272-2400629 (Counterclockwise)

Preceding gene: 150397522

Following gene: 150397519

Centisome position: 63.52

GC content: 62.29

Gene sequence:

>1644_bases
ATGGCGGCCCCATCGGAATCGGATGACAACTGGTGGCGCGGAGCGGTCATCTATCAGGTCTATCCGCGATCTTTCCAGGA
CACCGACGGCGACGGAATGGGTGACCTTCGCGGCGTTACCCGGCGGCTTCCGCATATCGCCTCGCTCGGTGTCGATGCCA
TCTGGCTCTCGCCTTTCTTCACCTCGCCCCAAGCGGACATGGGCTATGACGTTTCGGATTATTGCGACGTCGATCCGATG
TTCGGCACGCTCGACGATTTCGACGAGATGATGGCGGAAGCCCATCGGCTGGGACTCAAGGTGATTATCGACCAGGTGAT
CTCGCACACATCCGACCGGCATCCCTGGTTCATGGAGAGCCGTTCGAGCAGGACGAATGCCAAGGCGGACTGGTATGTAT
GGGCCGACCCGAAACCGGACGGCACGGCGCCGAACAACTGGCTTTCAATCTTCGGCGGCCCGGGCTGGGAGTGGGATGGG
GTCCGCAGGCAATATTACCTGCACAATTTCCTTTCGTCGCAGCCCGATCTCAATTTCCATAACCCTGAAGTTCAGGAAGC
TGTGCTGGCGACGGTCCGCTTCTGGCTGGACCGAGGCGTGGACGGTTTCAGGCTCGATACGGCGAACTTCTATTTCCACG
ATCGGCTTTTGCGGGACAACCCGCCGCTGGTGCCGGATCCGGACGCGACGAGCCGCGACGCGCCAGAGGTCAATCCTTAC
GGCATGCAGAACCACCTCTATGACAAGACGCAGCCGGAAAACATCGATTTCCTGCGCCGGTTCCGGGCGCTGCTCGATGA
ATATGGCGGCCGCGCGACGGTGGGCGAAATCGGCGACGGATCACGCTCGCTGCAGACGGTTGCCGCCTATACGAGTGGCG
GCGACAAGCTGCACATGTGCTACACATTCGACCTGCTCGGACCGGAATTCACCGCGCGTCACATCCGCCGCTGCGTTGAG
AGTTTCCAGGCAACGGTGGCCGACGGCTGGGTCTGCTGGGCCTTTTCCAATCACGACGTCATGCGCCATGTCAGCCGCTT
TGCGTTGCGCGAGGCCGACCGTGCGCGCGTGGCGAAGCTCGCGATTTCGTTGCTGGCCACTCTGCGGGGCACCATCTGCC
TTTACCAGGGGGAGGAATTGGGATTGCCGGAAGCCGACCTTGCCTTCGAAGAACTGCGCGATCCATACGGAATCCGCTTC
TGGCCCGCCTTTGCCGGACGCGACGGATGCCGGACGCCGATGGTATGGGGAAGTGAACTGAAGAATGCCGGCTTTTCCGA
CGGCGTTCCCTGGCTGCCGGTGCGCGAAGGGCACCGGACGCTGGCGGTGGACGCGCAGGATGGTGTCGAGGGTTCCGTGC
TCGAGCACTATCGAGACACCCTTGAATTCCGCCGGAGGCGGCCGGCGCTCGTCGATGGAGACATGGCGTTCCTCGGCACC
AACCAGGATATTCTGGTCTTCACGCGGGAGAAAGACGGCGATCGGCTGCTGTTCGTCTTCAACCTGACGCCGGAACCGCA
GACGTTTCAGCTTCCGGCAACGCTCGTGGCCGAGCCGCTGCCGATGCCCGGATTCGCGCCGCCTTTCGCGGACAATGCGA
TCACGCTTGCTGCGCTCGACGTTTTCTGCGGCAGGCTGCGTTAG

Upstream 100 bases:

>100_bases
CGATGTCGCCGAGCGAGCAAGCGAATGTTGGGGGCGGCGTTTACTCGAAGCGGCGGTGAAGATAAATCTGAATTGCTGAA
CCAAACACGGAGACCTGCGC

Downstream 100 bases:

>100_bases
GTCATCAGGTCAGGCTCTGTCGTGCGGGCTTTCATGCTCTGAAACACGATCAGGTTGGGCGCATTGCGGTTCGGGTCTCT
CGAAACTGGCGGATCACCTT

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MAAPSESDDNWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFFTSPQADMGYDVSDYCDVDPM
FGTLDDFDEMMAEAHRLGLKVIIDQVISHTSDRHPWFMESRSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDG
VRRQYYLHNFLSSQPDLNFHNPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPY
GMQNHLYDKTQPENIDFLRRFRALLDEYGGRATVGEIGDGSRSLQTVAAYTSGGDKLHMCYTFDLLGPEFTARHIRRCVE
SFQATVADGWVCWAFSNHDVMRHVSRFALREADRARVAKLAISLLATLRGTICLYQGEELGLPEADLAFEELRDPYGIRF
WPAFAGRDGCRTPMVWGSELKNAGFSDGVPWLPVREGHRTLAVDAQDGVEGSVLEHYRDTLEFRRRRPALVDGDMAFLGT
NQDILVFTREKDGDRLLFVFNLTPEPQTFQLPATLVAEPLPMPGFAPPFADNAITLAALDVFCGRLR

Sequences:

>Translated_547_residues
MAAPSESDDNWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFFTSPQADMGYDVSDYCDVDPM
FGTLDDFDEMMAEAHRLGLKVIIDQVISHTSDRHPWFMESRSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDG
VRRQYYLHNFLSSQPDLNFHNPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPY
GMQNHLYDKTQPENIDFLRRFRALLDEYGGRATVGEIGDGSRSLQTVAAYTSGGDKLHMCYTFDLLGPEFTARHIRRCVE
SFQATVADGWVCWAFSNHDVMRHVSRFALREADRARVAKLAISLLATLRGTICLYQGEELGLPEADLAFEELRDPYGIRF
WPAFAGRDGCRTPMVWGSELKNAGFSDGVPWLPVREGHRTLAVDAQDGVEGSVLEHYRDTLEFRRRRPALVDGDMAFLGT
NQDILVFTREKDGDRLLFVFNLTPEPQTFQLPATLVAEPLPMPGFAPPFADNAITLAALDVFCGRLR
>Mature_546_residues
AAPSESDDNWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFFTSPQADMGYDVSDYCDVDPMF
GTLDDFDEMMAEAHRLGLKVIIDQVISHTSDRHPWFMESRSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGV
RRQYYLHNFLSSQPDLNFHNPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPYG
MQNHLYDKTQPENIDFLRRFRALLDEYGGRATVGEIGDGSRSLQTVAAYTSGGDKLHMCYTFDLLGPEFTARHIRRCVES
FQATVADGWVCWAFSNHDVMRHVSRFALREADRARVAKLAISLLATLRGTICLYQGEELGLPEADLAFEELRDPYGIRFW
PAFAGRDGCRTPMVWGSELKNAGFSDGVPWLPVREGHRTLAVDAQDGVEGSVLEHYRDTLEFRRRRPALVDGDMAFLGTN
QDILVFTREKDGDRLLFVFNLTPEPQTFQLPATLVAEPLPMPGFAPPFADNAITLAALDVFCGRLR

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=536, Percent_Identity=32.6492537313433, Blast_Score=278, Evalue=7e-75,
Organism=Escherichia coli, GI1790687, Length=534, Percent_Identity=36.3295880149813, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1786604, Length=495, Percent_Identity=23.030303030303, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI32565753, Length=496, Percent_Identity=26.008064516129, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25147709, Length=491, Percent_Identity=25.8655804480652, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6322245, Length=555, Percent_Identity=34.4144144144144, Blast_Score=303, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6321731, Length=556, Percent_Identity=34.1726618705036, Blast_Score=298, Evalue=2e-81,
Organism=Saccharomyces cerevisiae, GI6319776, Length=556, Percent_Identity=34.1726618705036, Blast_Score=297, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6321726, Length=560, Percent_Identity=34.1071428571429, Blast_Score=288, Evalue=2e-78,
Organism=Saccharomyces cerevisiae, GI6324416, Length=558, Percent_Identity=34.9462365591398, Blast_Score=286, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6322241, Length=558, Percent_Identity=34.5878136200717, Blast_Score=283, Evalue=4e-77,
Organism=Saccharomyces cerevisiae, GI6322021, Length=558, Percent_Identity=34.5878136200717, Blast_Score=283, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24586587, Length=502, Percent_Identity=37.0517928286853, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24583745, Length=534, Percent_Identity=36.1423220973783, Blast_Score=309, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24586599, Length=541, Percent_Identity=34.0110905730129, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24583747, Length=544, Percent_Identity=35.8455882352941, Blast_Score=301, Evalue=7e-82,
Organism=Drosophila melanogaster, GI24583749, Length=537, Percent_Identity=35.9404096834264, Blast_Score=301, Evalue=8e-82,
Organism=Drosophila melanogaster, GI24586589, Length=526, Percent_Identity=35.171102661597, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI45549022, Length=527, Percent_Identity=33.965844402277, Blast_Score=295, Evalue=4e-80,
Organism=Drosophila melanogaster, GI24586597, Length=535, Percent_Identity=34.5794392523364, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24586591, Length=529, Percent_Identity=35.538752362949, Blast_Score=294, Evalue=1e-79,
Organism=Drosophila melanogaster, GI221330053, Length=510, Percent_Identity=34.3137254901961, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI24586593, Length=518, Percent_Identity=33.5907335907336, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI281360393, Length=460, Percent_Identity=30.8695652173913, Blast_Score=206, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61849; Mature: 61718

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPSESDDNWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFF
CCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEECCCC
TSPQADMGYDVSDYCDVDPMFGTLDDFDEMMAEAHRLGLKVIIDQVISHTSDRHPWFMES
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGVRRQYYLHNFLSSQPDLNFH
CCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPY
CHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
GMQNHLYDKTQPENIDFLRRFRALLDEYGGRATVGEIGDGSRSLQTVAAYTSGGDKLHMC
CCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEEECCCCEEEEE
YTFDLLGPEFTARHIRRCVESFQATVADGWVCWAFSNHDVMRHVSRFALREADRARVAKL
EEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AISLLATLRGTICLYQGEELGLPEADLAFEELRDPYGIRFWPAFAGRDGCRTPMVWGSEL
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECHHH
KNAGFSDGVPWLPVREGHRTLAVDAQDGVEGSVLEHYRDTLEFRRRRPALVDGDMAFLGT
CCCCCCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEC
NQDILVFTREKDGDRLLFVFNLTPEPQTFQLPATLVAEPLPMPGFAPPFADNAITLAALD
CCEEEEEEECCCCCEEEEEEECCCCCCEEECCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VFCGRLR
HHHHCCC
>Mature Secondary Structure 
AAPSESDDNWWRGAVIYQVYPRSFQDTDGDGMGDLRGVTRRLPHIASLGVDAIWLSPFF
CCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEECCCC
TSPQADMGYDVSDYCDVDPMFGTLDDFDEMMAEAHRLGLKVIIDQVISHTSDRHPWFMES
CCCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
RSSRTNAKADWYVWADPKPDGTAPNNWLSIFGGPGWEWDGVRRQYYLHNFLSSQPDLNFH
CCCCCCCCCCEEEECCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
NPEVQEAVLATVRFWLDRGVDGFRLDTANFYFHDRLLRDNPPLVPDPDATSRDAPEVNPY
CHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEHHHHCCCCCCCCCCCCCCCCCCCCCCC
GMQNHLYDKTQPENIDFLRRFRALLDEYGGRATVGEIGDGSRSLQTVAAYTSGGDKLHMC
CCHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHEEEEEECCCCEEEEE
YTFDLLGPEFTARHIRRCVESFQATVADGWVCWAFSNHDVMRHVSRFALREADRARVAKL
EEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
AISLLATLRGTICLYQGEELGLPEADLAFEELRDPYGIRFWPAFAGRDGCRTPMVWGSEL
HHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEECHHH
KNAGFSDGVPWLPVREGHRTLAVDAQDGVEGSVLEHYRDTLEFRRRRPALVDGDMAFLGT
CCCCCCCCCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEECCCEEEEEC
NQDILVFTREKDGDRLLFVFNLTPEPQTFQLPATLVAEPLPMPGFAPPFADNAITLAALD
CCEEEEEEECCCCCEEEEEEECCCCCCEEECCHHHHCCCCCCCCCCCCCCCCCCHHHHHH
VFCGRLR
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]