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The map label for this gene is queF [H]

Identifier: 150397493

GI number: 150397493

Start: 2368135

End: 2368599

Strand: Reverse

Name: queF [H]

Synonym: Smed_2293

Alternate gene names: 150397493

Gene position: 2368599-2368135 (Counterclockwise)

Preceding gene: 150397494

Following gene: 150397492

Centisome position: 62.63

GC content: 61.94

Gene sequence:

>465_bases
ATGACGAAGACGGACGTATCAGGACTTTCCCAGCTCGGGACGAAGGTCGATCTGCCCCAGAACCCGGAAGAGGCGGTGCT
CGAAAGGGTGCCGAGCGGCCATGGCGGCACCGATTTCGTCGTCCGCTTCACGGCGCCGGAGTTCACCTCTCTTTGCCCGA
TGACAGGGCAGCCCGACTTCGCCCACCTCGTAATCGATTACGTGCCGGACGGCTGGCTGGTCGAGTCGAAATCTCTGAAG
CTTTTCCTGCACTCCTTCCGCAATCACGGCGCTTTTCACGAGGATTGCACGATCGATATCGCCAAGCGGCTGGTTTCGCT
GCTTTCGCCGAAGTGGCTTCGGATCGGCGCCTATTGGTATCCCCGCGGTGGAATTCCGATCGACGTTTTCTGGCAGACCG
GAAGCCCGCCGGAAGGTGTCTGGCTGCCCGATCAGGGCGTGCCGACCTATCGCGGGCGGGGCTGA

Upstream 100 bases:

>100_bases
TTCGCGCCGTGCATCCGGGTGCGAGGGTCGCCATTCACGTCGAACCGGAAGGCGAAAAGGCGCACGGCGTGCGCGTTAAA
GTGAGCGGAGCATCACGGAA

Downstream 100 bases:

>100_bases
CATTTGCTGAATTGCGCGGGCAGCTTCCATGATAAATCGCCAGTTTGCCGCCGAAAGGCTTCTGCCGTCGCCGCCCGCGG
GTAGGGAATGGCTGTCGGTT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MTKTDVSGLSQLGTKVDLPQNPEEAVLERVPSGHGGTDFVVRFTAPEFTSLCPMTGQPDFAHLVIDYVPDGWLVESKSLK
LFLHSFRNHGAFHEDCTIDIAKRLVSLLSPKWLRIGAYWYPRGGIPIDVFWQTGSPPEGVWLPDQGVPTYRGRG

Sequences:

>Translated_154_residues
MTKTDVSGLSQLGTKVDLPQNPEEAVLERVPSGHGGTDFVVRFTAPEFTSLCPMTGQPDFAHLVIDYVPDGWLVESKSLK
LFLHSFRNHGAFHEDCTIDIAKRLVSLLSPKWLRIGAYWYPRGGIPIDVFWQTGSPPEGVWLPDQGVPTYRGRG
>Mature_153_residues
TKTDVSGLSQLGTKVDLPQNPEEAVLERVPSGHGGTDFVVRFTAPEFTSLCPMTGQPDFAHLVIDYVPDGWLVESKSLKL
FLHSFRNHGAFHEDCTIDIAKRLVSLLSPKWLRIGAYWYPRGGIPIDVFWQTGSPPEGVWLPDQGVPTYRGRG

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 17122; Mature: 16990

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTDVSGLSQLGTKVDLPQNPEEAVLERVPSGHGGTDFVVRFTAPEFTSLCPMTGQPDF
CCCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCCCCCCCC
AHLVIDYVPDGWLVESKSLKLFLHSFRNHGAFHEDCTIDIAKRLVSLLSPKWLRIGAYWY
EEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEE
PRGGIPIDVFWQTGSPPEGVWLPDQGVPTYRGRG
CCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure 
TKTDVSGLSQLGTKVDLPQNPEEAVLERVPSGHGGTDFVVRFTAPEFTSLCPMTGQPDF
CCCCCHHHHHCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCHHCCCCCCCCCC
AHLVIDYVPDGWLVESKSLKLFLHSFRNHGAFHEDCTIDIAKRLVSLLSPKWLRIGAYWY
EEEEEEECCCCEEEECCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEEE
PRGGIPIDVFWQTGSPPEGVWLPDQGVPTYRGRG
CCCCCEEEEEECCCCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]