Click here to switch to the map view.

The map label for this gene is aphE [H]

Identifier: 150397492

GI number: 150397492

Start: 2367285

End: 2368106

Strand: Reverse

Name: aphE [H]

Synonym: Smed_2292

Alternate gene names: 150397492

Gene position: 2368106-2367285 (Counterclockwise)

Preceding gene: 150397493

Following gene: 150397489

Centisome position: 62.62

GC content: 64.11

Gene sequence:

>822_bases
ATGATAAATCGCCAGTTTGCCGCCGAAAGGCTTCTGCCGTCGCCGCCCGCGGGTAGGGAATGGCTGTCGGTTCGCCACGG
CGAATCCGGCGACCGGGTTTATGCGCGCAGCGACGGCGCCGCCTATGCAAAGATTGCGAGCGGCAAAGCCGCGGCACTGC
TCGAAGGCGAGCGCGATCGCGCTGCGTGGCTGGCGGCGTTCGGCATCGGCTCACCGGCCGTTTACGACTGGATCGCCGAC
AGCCACGAGGCGTGTCTCGTGCTCAGCGCGGTTCCGGGCGTGCCGGCGAGCGATCTGGCCGTCCCCGATCTCATGAAAGC
ATGGCCCACGATCATGCGGCAGTTTAAGGCCCTGCACGAGCTGCCCAGCGAATCATGCCCGTTCGAACGGCGCCTCGCTT
CGATGTTCGAGCATGCCACGGACGTCGTTGCCCGCGAAGCGGTCAACCCGGATTTCCTTGCTCCCGAGGATCAGAACGTA
CCACCGAGCAAATTGCTTCTGGCTCTGGGGGTGGAGTTGCCGAAGCGCCTGGCCGAGGAGGCCCGCGATCTCGTGATCTG
CCATGGCGACGCATGCTTGCCGAATTTCATGGTCGACCCGGAAACGCAGCGCTGCACAGGCCTCATCGACCTCGGCCGGC
TCGGAACGGCGGATCGATATGTCGATTTTTCGCTTCTTCTCGGAAATGCCCGGGAAAGCTGGACGACGCCGGGCCAAGCG
CAGATGGCACGGGACTCGCTCTTCGGCATTCACGGGATCGCCAATCCGGACGAAGGCCGGCTCGCCTTCTATCTTCGTTT
GGATCCGCTGACCTGGGGATGA

Upstream 100 bases:

>100_bases
CAGACCGGAAGCCCGCCGGAAGGTGTCTGGCTGCCCGATCAGGGCGTGCCGACCTATCGCGGGCGGGGCTGACATTTGCT
GAATTGCGCGGGCAGCTTCC

Downstream 100 bases:

>100_bases
AGAAGTGCTTTGGACAGTCCAACCTTCTCCAGATCGATCCTGAGTATGGGGTCGAAGGCGACCTTTCTCCAACGCACCTG
GTAGCCGAGATGCTGGTAGA

Product: aminoglycoside phosphotransferase

Products: NA

Alternate protein names: Streptomycin 3''-phosphotransferase; Streptomycin 6-kinase; Streptomycin 6-phosphotransferase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MINRQFAAERLLPSPPAGREWLSVRHGESGDRVYARSDGAAYAKIASGKAAALLEGERDRAAWLAAFGIGSPAVYDWIAD
SHEACLVLSAVPGVPASDLAVPDLMKAWPTIMRQFKALHELPSESCPFERRLASMFEHATDVVAREAVNPDFLAPEDQNV
PPSKLLLALGVELPKRLAEEARDLVICHGDACLPNFMVDPETQRCTGLIDLGRLGTADRYVDFSLLLGNARESWTTPGQA
QMARDSLFGIHGIANPDEGRLAFYLRLDPLTWG

Sequences:

>Translated_273_residues
MINRQFAAERLLPSPPAGREWLSVRHGESGDRVYARSDGAAYAKIASGKAAALLEGERDRAAWLAAFGIGSPAVYDWIAD
SHEACLVLSAVPGVPASDLAVPDLMKAWPTIMRQFKALHELPSESCPFERRLASMFEHATDVVAREAVNPDFLAPEDQNV
PPSKLLLALGVELPKRLAEEARDLVICHGDACLPNFMVDPETQRCTGLIDLGRLGTADRYVDFSLLLGNARESWTTPGQA
QMARDSLFGIHGIANPDEGRLAFYLRLDPLTWG
>Mature_273_residues
MINRQFAAERLLPSPPAGREWLSVRHGESGDRVYARSDGAAYAKIASGKAAALLEGERDRAAWLAAFGIGSPAVYDWIAD
SHEACLVLSAVPGVPASDLAVPDLMKAWPTIMRQFKALHELPSESCPFERRLASMFEHATDVVAREAVNPDFLAPEDQNV
PPSKLLLALGVELPKRLAEEARDLVICHGDACLPNFMVDPETQRCTGLIDLGRLGTADRYVDFSLLLGNARESWTTPGQA
QMARDSLFGIHGIANPDEGRLAFYLRLDPLTWG

Specific function: The aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation [H]

COG id: COG3231

COG function: function code J; Aminoglycoside phosphotransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aminoglycoside phosphotransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002575
- InterPro:   IPR011009 [H]

Pfam domain/function: PF01636 APH [H]

EC number: =2.7.1.87 [H]

Molecular weight: Translated: 29788; Mature: 29788

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRQFAAERLLPSPPAGREWLSVRHGESGDRVYARSDGAAYAKIASGKAAALLEGERDR
CCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCHH
AAWLAAFGIGSPAVYDWIADSHEACLVLSAVPGVPASDLAVPDLMKAWPTIMRQFKALHE
HHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPSESCPFERRLASMFEHATDVVAREAVNPDFLAPEDQNVPPSKLLLALGVELPKRLAEE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARDLVICHGDACLPNFMVDPETQRCTGLIDLGRLGTADRYVDFSLLLGNARESWTTPGQA
HCCEEEECCCCCCCCCEECCCHHHHHHHHHHCCCCCCHHHEEEEEEECCCHHCCCCCCHH
QMARDSLFGIHGIANPDEGRLAFYLRLDPLTWG
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MINRQFAAERLLPSPPAGREWLSVRHGESGDRVYARSDGAAYAKIASGKAAALLEGERDR
CCCHHHHHHHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEECCCCEEEEECCCCHH
AAWLAAFGIGSPAVYDWIADSHEACLVLSAVPGVPASDLAVPDLMKAWPTIMRQFKALHE
HHHHHHHCCCCCHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LPSESCPFERRLASMFEHATDVVAREAVNPDFLAPEDQNVPPSKLLLALGVELPKRLAEE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ARDLVICHGDACLPNFMVDPETQRCTGLIDLGRLGTADRYVDFSLLLGNARESWTTPGQA
HCCEEEECCCCCCCCCEECCCHHHHHHHHHHCCCCCCHHHEEEEEEECCCHHCCCCCCHH
QMARDSLFGIHGIANPDEGRLAFYLRLDPLTWG
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2167474; 2844130; 2821169 [H]