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The map label for this gene is pth [H]

Identifier: 150397464

GI number: 150397464

Start: 2342028

End: 2342774

Strand: Direct

Name: pth [H]

Synonym: Smed_2264

Alternate gene names: 150397464

Gene position: 2342028-2342774 (Clockwise)

Preceding gene: 150397463

Following gene: 150397466

Centisome position: 61.93

GC content: 59.84

Gene sequence:

>747_bases
ATGCAAACGCATCAGGAGTTTGGCCGCATGCTGATCATCGCAGGACTTGGCAATCCGGGTCCTAAATACGCCGGTAACCG
CCACAATATCGGCTTCATGGCCGTAGACGCCATTCAGGGACGCCAGGGCTTTTCTTCGTGGTCGAAGAAATTCAAGTCGG
AGATCTCCGAAGGCGAGATCGACGGCGAGCGCGTCCTGCTGATGAAGCCGCAGACCTTCATGAACCTGTCCGGCGAAGCC
TTGGGCGATGCGATGCGTTTCTACAAGCTCGCACCGAAGGACATCGTCGTCATCTATGACGAGCTGGACCTGCCCGCCGG
CAAGGCGCGGATCAAGACTGGCGGCGGCCATGGCGGGCACAACGGCATCAAGTCGATCGACGCTCATTGCGGTAGAGAGT
ACCGCCGCCTGCGGCTCGGCATCGGCCATCCCGGAGCCAAGGATCTGGTCCACGCGCATGTGCTCGGCGATTTTGCCAAA
GCGGACCGGGCCTGGGTCTCGCCACTTCTCGAGGCGATCGCCGACAATGCCGGAATGCTGGTGAAGGGCGAAGATTCACA
GCTCATGAACAAGATCGCACTGGCAACCGGAGGCAAACCCGAGATGGATAAGCCGCCGACACCGAAGAAGCAAACGGCGC
AATCCCATATCCATCAGGCTCGCGCCCCGGTTCAACCGAAGAAGCTGCCAACTACGGGGCCCATGGCCGATATGCTGAAG
AAGCTGTTCGGGACGAAAGGGGAATGA

Upstream 100 bases:

>100_bases
ACCCGAGGACTAACCCTCTCCCCGCCGGCGGGGGAGCGCGCCGCGAGTCCCTGATGCCATCAAAAATGGCGAGAAGGTGC
CGGCAGGCGGATGAGGGCAC

Downstream 100 bases:

>100_bases
TAAGCGGACCGCCGCCGCTGCCTCAATCCTCCGGCTCCGCCGTGAACAGCAGCGGAAAGCCTGCCTCCTTGCCAAGGTCG
GTCGCCTCCTTGGCCTTTGT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MQTHQEFGRMLIIAGLGNPGPKYAGNRHNIGFMAVDAIQGRQGFSSWSKKFKSEISEGEIDGERVLLMKPQTFMNLSGEA
LGDAMRFYKLAPKDIVVIYDELDLPAGKARIKTGGGHGGHNGIKSIDAHCGREYRRLRLGIGHPGAKDLVHAHVLGDFAK
ADRAWVSPLLEAIADNAGMLVKGEDSQLMNKIALATGGKPEMDKPPTPKKQTAQSHIHQARAPVQPKKLPTTGPMADMLK
KLFGTKGE

Sequences:

>Translated_248_residues
MQTHQEFGRMLIIAGLGNPGPKYAGNRHNIGFMAVDAIQGRQGFSSWSKKFKSEISEGEIDGERVLLMKPQTFMNLSGEA
LGDAMRFYKLAPKDIVVIYDELDLPAGKARIKTGGGHGGHNGIKSIDAHCGREYRRLRLGIGHPGAKDLVHAHVLGDFAK
ADRAWVSPLLEAIADNAGMLVKGEDSQLMNKIALATGGKPEMDKPPTPKKQTAQSHIHQARAPVQPKKLPTTGPMADMLK
KLFGTKGE
>Mature_248_residues
MQTHQEFGRMLIIAGLGNPGPKYAGNRHNIGFMAVDAIQGRQGFSSWSKKFKSEISEGEIDGERVLLMKPQTFMNLSGEA
LGDAMRFYKLAPKDIVVIYDELDLPAGKARIKTGGGHGGHNGIKSIDAHCGREYRRLRLGIGHPGAKDLVHAHVLGDFAK
ADRAWVSPLLEAIADNAGMLVKGEDSQLMNKIALATGGKPEMDKPPTPKKQTAQSHIHQARAPVQPKKLPTTGPMADMLK
KLFGTKGE

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis [H]

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family [H]

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=32.5581395348837, Blast_Score=102, Evalue=4e-22,
Organism=Escherichia coli, GI1787455, Length=148, Percent_Identity=47.2972972972973, Blast_Score=103, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321983, Length=145, Percent_Identity=31.0344827586207, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001328
- InterPro:   IPR018171 [H]

Pfam domain/function: PF01195 Pept_tRNA_hydro [H]

EC number: =3.1.1.29 [H]

Molecular weight: Translated: 26879; Mature: 26879

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1 ; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTHQEFGRMLIIAGLGNPGPKYAGNRHNIGFMAVDAIQGRQGFSSWSKKFKSEISEGEI
CCCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC
DGERVLLMKPQTFMNLSGEALGDAMRFYKLAPKDIVVIYDELDLPAGKARIKTGGGHGGH
CCCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
NGIKSIDAHCGREYRRLRLGIGHPGAKDLVHAHVLGDFAKADRAWVSPLLEAIADNAGML
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VKGEDSQLMNKIALATGGKPEMDKPPTPKKQTAQSHIHQARAPVQPKKLPTTGPMADMLK
EECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KLFGTKGE
HHHCCCCC
>Mature Secondary Structure
MQTHQEFGRMLIIAGLGNPGPKYAGNRHNIGFMAVDAIQGRQGFSSWSKKFKSEISEGEI
CCCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCC
DGERVLLMKPQTFMNLSGEALGDAMRFYKLAPKDIVVIYDELDLPAGKARIKTGGGHGGH
CCCEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEECCCCCCCC
NGIKSIDAHCGREYRRLRLGIGHPGAKDLVHAHVLGDFAKADRAWVSPLLEAIADNAGML
CHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VKGEDSQLMNKIALATGGKPEMDKPPTPKKQTAQSHIHQARAPVQPKKLPTTGPMADMLK
EECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
KLFGTKGE
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]