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The map label for this gene is yafC [H]

Identifier: 150397448

GI number: 150397448

Start: 2325437

End: 2326399

Strand: Reverse

Name: yafC [H]

Synonym: Smed_2248

Alternate gene names: 150397448

Gene position: 2326399-2325437 (Counterclockwise)

Preceding gene: 150397449

Following gene: 150397447

Centisome position: 61.51

GC content: 62.72

Gene sequence:

>963_bases
ATGGATCGACAGGAGGCGATGGCGGTGTTTCTCGCCGTTGTCGAGGAGGGGGACTTCTCGGCGGCTGCCCGGCGGCTGCG
AATGACGCCATCCGCCGTCAGCAAAATCGTTGGCCGGCTCGAATCCCGGCTCGGAGTTCGGCTGTTGCAGCGCTCGACCC
GCAGTATCAGCCTTACGGCCGAGGGAAATTCCTATTCGGAGTCGGTCCGGCGCATTCTCGGCGATATGGAAGAGGCCGAG
CTGGCGATTCAGCCGGGCGCCGAGCCGCGCGGCCGATTACGCGTCAGTCTTCCAAGTGCATTCGGCCATCGTCTGATCGT
CCCGATGCTGCCGGCCTTCATCGGACGCCATCCGGCCATCGAACTTGAACTGGATTTCACCGACGCGATCGTCGATCTCG
TAAATGGCGATGCGCACGTGGCGGTGCGTGTGGCTGCCCAAGGCGACTCCACGCTCGTCACGCGGCGTCTGGCCCCCAAT
AGGCGCGTCATCTGCGGTGCTCCTGATTACTTCGAGCGTCATGGTGTCCCCATGAGGCCCGACGACCTCCTGAAGCACAT
CTGCCTCGGCATTTCGTCGCGTGGTGGGCTCAACCTGTGGGAGTTCGATGAAGCTGGCGGCCGCCGGTCGATGCGCATTC
GAAGTTCGATCGAGGCCAACAGCACGGAAGCACTGCGCCGGCTTGCGCTTGCCGGAATGGGGATTGTGCGGCTTTCGGAG
ATGCTGGTCGGGCAGGACATCAGGGCTGGGCGCCTGAAGCCGGTGCTCACGGACTGCAATCATGCCGAGGCCGAGCCGAT
CAGCGTCGTCTATCCGCATCGCCGGTTCCTCTCGCCACGGGTGCGCGTATTCGTCGATTTCCTCGCTGAACAATTTGCAA
AATCGATCGCAGAAGGAGGCGATGCTGCCCGTGTGATACGAGGTACATCCGGCGGTCTTCCCGAATCCGACGCAATGCGT
TAA

Upstream 100 bases:

>100_bases
ATTCACCTTCTGACAATTGCCATCGGATGATAAGGCTTAACTTTCTCGACGATAATCCGTATTGATTTCGTTATATCTGT
GACGAAATGGAATAGATGCC

Downstream 100 bases:

>100_bases
GCCTGCTCCGAGTTCCTCAGCAGGGAATCGCCATGGCGGAAATCATCGAATTTGCCGATTTTGAACGCGTCGACATTCGT
GTCGGGACAATCATCGAGGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDRQEAMAVFLAVVEEGDFSAAARRLRMTPSAVSKIVGRLESRLGVRLLQRSTRSISLTAEGNSYSESVRRILGDMEEAE
LAIQPGAEPRGRLRVSLPSAFGHRLIVPMLPAFIGRHPAIELELDFTDAIVDLVNGDAHVAVRVAAQGDSTLVTRRLAPN
RRVICGAPDYFERHGVPMRPDDLLKHICLGISSRGGLNLWEFDEAGGRRSMRIRSSIEANSTEALRRLALAGMGIVRLSE
MLVGQDIRAGRLKPVLTDCNHAEAEPISVVYPHRRFLSPRVRVFVDFLAEQFAKSIAEGGDAARVIRGTSGGLPESDAMR

Sequences:

>Translated_320_residues
MDRQEAMAVFLAVVEEGDFSAAARRLRMTPSAVSKIVGRLESRLGVRLLQRSTRSISLTAEGNSYSESVRRILGDMEEAE
LAIQPGAEPRGRLRVSLPSAFGHRLIVPMLPAFIGRHPAIELELDFTDAIVDLVNGDAHVAVRVAAQGDSTLVTRRLAPN
RRVICGAPDYFERHGVPMRPDDLLKHICLGISSRGGLNLWEFDEAGGRRSMRIRSSIEANSTEALRRLALAGMGIVRLSE
MLVGQDIRAGRLKPVLTDCNHAEAEPISVVYPHRRFLSPRVRVFVDFLAEQFAKSIAEGGDAARVIRGTSGGLPESDAMR
>Mature_320_residues
MDRQEAMAVFLAVVEEGDFSAAARRLRMTPSAVSKIVGRLESRLGVRLLQRSTRSISLTAEGNSYSESVRRILGDMEEAE
LAIQPGAEPRGRLRVSLPSAFGHRLIVPMLPAFIGRHPAIELELDFTDAIVDLVNGDAHVAVRVAAQGDSTLVTRRLAPN
RRVICGAPDYFERHGVPMRPDDLLKHICLGISSRGGLNLWEFDEAGGRRSMRIRSSIEANSTEALRRLALAGMGIVRLSE
MLVGQDIRAGRLKPVLTDCNHAEAEPISVVYPHRRFLSPRVRVFVDFLAEQFAKSIAEGGDAARVIRGTSGGLPESDAMR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=288, Percent_Identity=37.8472222222222, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=34.5890410958904, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI87081978, Length=291, Percent_Identity=32.3024054982818, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=32.0945945945946, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=27.8145695364238, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=29.7658862876254, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787128, Length=291, Percent_Identity=28.5223367697594, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=26.4705882352941, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786448, Length=299, Percent_Identity=27.7591973244147, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI157672245, Length=117, Percent_Identity=36.7521367521368, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI87082132, Length=297, Percent_Identity=23.9057239057239, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=23.448275862069, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35052; Mature: 35052

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRQEAMAVFLAVVEEGDFSAAARRLRMTPSAVSKIVGRLESRLGVRLLQRSTRSISLTA
CCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EGNSYSESVRRILGDMEEAELAIQPGAEPRGRLRVSLPSAFGHRLIVPMLPAFIGRHPAI
CCCCHHHHHHHHHCCHHHHEEEECCCCCCCCCEEEECCHHCCCEEHHHHHHHHHCCCCEE
ELELDFTDAIVDLVNGDAHVAVRVAAQGDSTLVTRRLAPNRRVICGAPDYFERHGVPMRP
EEEECHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHCCCCCCH
DDLLKHICLGISSRGGLNLWEFDEAGGRRSMRIRSSIEANSTEALRRLALAGMGIVRLSE
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
MLVGQDIRAGRLKPVLTDCNHAEAEPISVVYPHRRFLSPRVRVFVDFLAEQFAKSIAEGG
HHHCCCCCCCCCCHHHCCCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHCCC
DAARVIRGTSGGLPESDAMR
CCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MDRQEAMAVFLAVVEEGDFSAAARRLRMTPSAVSKIVGRLESRLGVRLLQRSTRSISLTA
CCHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
EGNSYSESVRRILGDMEEAELAIQPGAEPRGRLRVSLPSAFGHRLIVPMLPAFIGRHPAI
CCCCHHHHHHHHHCCHHHHEEEECCCCCCCCCEEEECCHHCCCEEHHHHHHHHHCCCCEE
ELELDFTDAIVDLVNGDAHVAVRVAAQGDSTLVTRRLAPNRRVICGAPDYFERHGVPMRP
EEEECHHHHHHHHHCCCCEEEEEEEECCCCEEEEEECCCCCEEEECCCCHHHHCCCCCCH
DDLLKHICLGISSRGGLNLWEFDEAGGRRSMRIRSSIEANSTEALRRLALAGMGIVRLSE
HHHHHHHHHCCCCCCCCCEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHCCHHHHHHH
MLVGQDIRAGRLKPVLTDCNHAEAEPISVVYPHRRFLSPRVRVFVDFLAEQFAKSIAEGG
HHHCCCCCCCCCCHHHCCCCCCCCCCEEEECCHHHHHCHHHHHHHHHHHHHHHHHHHCCC
DAARVIRGTSGGLPESDAMR
CCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]