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The map label for this gene is yafC [C]

Identifier: 150397430

GI number: 150397430

Start: 2310883

End: 2311773

Strand: Reverse

Name: yafC [C]

Synonym: Smed_2230

Alternate gene names: 150397430

Gene position: 2311773-2310883 (Counterclockwise)

Preceding gene: 150397433

Following gene: 150397428

Centisome position: 61.13

GC content: 62.74

Gene sequence:

>891_bases
ATGGCCAATCTCAACGACTTCACCTTTTTCGTTCATGTGGTGGATCACCATGGCTTTGCGCCGGCGGCGCGCGCGTTGAA
TGTGCCGAAGTCGACGCTCAGCAAGCGCGTAGCCGAGCTGGAAAGGAACCTCGGCGTCCGGCTGATCAACCGCACGTCCC
GTCATTTCGCGGTGACGGAAACCGGCAAGGATTTTTATCGCCATGCGGCGGCGATGATGATCGAGGCGGAAGCGGCGGAG
AATGTCGTCAGGGGCAGACTTGCCGAACCGAACGGCACGGTGAGGATCACGGCTTCGGTGCCGACCGCGCAGCTGTCGCT
GGCCCCGCTCCTGCCGCAACTCGCACTTGCCTATCCGAAGCTGCGCGTCACGCTGCATGCGACGGACCGCTTTGTCGATA
TCATTCAGGAAGGCTTCGATCTTGCCGTGCGCGACCATTTCGCGCCGCTGCCCGATTCCGGTCTCGTCCAGCGCCGGGTC
GGAACCCAGGCAAAGCTGATCGTCGCTTCACCGCTTTATTTGCACAGGAACGGAACTCCCGAACGCCCGGAGGATCTCGA
TCGTCATGACGGGCTGCTTACGTCGCTGGCGTCCGAAGGCTGGATGATGGAGCGCGCCGATGGGTCAACGGTATCGGTCT
CGCCGAAGCCGCGTTTCGTAGCGGATGAATCCCGCGTGCTGCGAGAAGCTGCGCTCGCCGGTCTCGGCATCACGACCTTG
CCCGGTAAGCTCTGTCAGCAGGAGATCGAAAGCGGTACGCTTATCCGCATCCTTCCCGGATGGACCGCCGGCAAGGTGGT
GACGACGATCCTGATGCCGCACCGGCGCGGGCAGCTTCCCTCCGTGCGGGCAACAGTCGATTTCATCGCTGAGCGTCTCG
TCGACGGCTGA

Upstream 100 bases:

>100_bases
TCTGTTCAACAATGGTGATGAACAGATGCACTTGTGGACGAATTTGTGCTAGATGGGCGTCTGTGGACCTATTGTCCATA
TTTGTGGATGATGAGTGCTC

Downstream 100 bases:

>100_bases
GGCAAGAAGGCCCGCACGAGGCGGGCCCTGAAAACGGCTCAGCGCTCGGCGAGTGCCGGTTCGCCTTTCTTCTCACGAAT
GAGGTTCAGGAAGCGGCGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MANLNDFTFFVHVVDHHGFAPAARALNVPKSTLSKRVAELERNLGVRLINRTSRHFAVTETGKDFYRHAAAMMIEAEAAE
NVVRGRLAEPNGTVRITASVPTAQLSLAPLLPQLALAYPKLRVTLHATDRFVDIIQEGFDLAVRDHFAPLPDSGLVQRRV
GTQAKLIVASPLYLHRNGTPERPEDLDRHDGLLTSLASEGWMMERADGSTVSVSPKPRFVADESRVLREAALAGLGITTL
PGKLCQQEIESGTLIRILPGWTAGKVVTTILMPHRRGQLPSVRATVDFIAERLVDG

Sequences:

>Translated_296_residues
MANLNDFTFFVHVVDHHGFAPAARALNVPKSTLSKRVAELERNLGVRLINRTSRHFAVTETGKDFYRHAAAMMIEAEAAE
NVVRGRLAEPNGTVRITASVPTAQLSLAPLLPQLALAYPKLRVTLHATDRFVDIIQEGFDLAVRDHFAPLPDSGLVQRRV
GTQAKLIVASPLYLHRNGTPERPEDLDRHDGLLTSLASEGWMMERADGSTVSVSPKPRFVADESRVLREAALAGLGITTL
PGKLCQQEIESGTLIRILPGWTAGKVVTTILMPHRRGQLPSVRATVDFIAERLVDG
>Mature_295_residues
ANLNDFTFFVHVVDHHGFAPAARALNVPKSTLSKRVAELERNLGVRLINRTSRHFAVTETGKDFYRHAAAMMIEAEAAEN
VVRGRLAEPNGTVRITASVPTAQLSLAPLLPQLALAYPKLRVTLHATDRFVDIIQEGFDLAVRDHFAPLPDSGLVQRRVG
TQAKLIVASPLYLHRNGTPERPEDLDRHDGLLTSLASEGWMMERADGSTVSVSPKPRFVADESRVLREAALAGLGITTLP
GKLCQQEIESGTLIRILPGWTAGKVVTTILMPHRRGQLPSVRATVDFIAERLVDG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=298, Percent_Identity=31.5436241610738, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789639, Length=264, Percent_Identity=31.8181818181818, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=28.125, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=27.5261324041812, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=28.6195286195286, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=26.8965517241379, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=27.5985663082437, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789173, Length=254, Percent_Identity=28.3464566929134, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788481, Length=265, Percent_Identity=26.4150943396226, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788706, Length=246, Percent_Identity=24.7967479674797, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788297, Length=152, Percent_Identity=30.9210526315789, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI87082132, Length=307, Percent_Identity=21.8241042345277, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32423; Mature: 32292

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLNDFTFFVHVVDHHGFAPAARALNVPKSTLSKRVAELERNLGVRLINRTSRHFAVTE
CCCCCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEC
TGKDFYRHAAAMMIEAEAAENVVRGRLAEPNGTVRITASVPTAQLSLAPLLPQLALAYPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCE
LRVTLHATDRFVDIIQEGFDLAVRDHFAPLPDSGLVQRRVGTQAKLIVASPLYLHRNGTP
EEEEEECHHHHHHHHHHCCCEEHHHCCCCCCCCCHHHHHCCCCEEEEEECCEEEECCCCC
ERPEDLDRHDGLLTSLASEGWMMERADGSTVSVSPKPRFVADESRVLREAALAGLGITTL
CCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEECCHHHHHHHHHHHCCCCCCC
PGKLCQQEIESGTLIRILPGWTAGKVVTTILMPHRRGQLPSVRATVDFIAERLVDG
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ANLNDFTFFVHVVDHHGFAPAARALNVPKSTLSKRVAELERNLGVRLINRTSRHFAVTE
CCCCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEEEC
TGKDFYRHAAAMMIEAEAAENVVRGRLAEPNGTVRITASVPTAQLSLAPLLPQLALAYPK
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCE
LRVTLHATDRFVDIIQEGFDLAVRDHFAPLPDSGLVQRRVGTQAKLIVASPLYLHRNGTP
EEEEEECHHHHHHHHHHCCCEEHHHCCCCCCCCCHHHHHCCCCEEEEEECCEEEECCCCC
ERPEDLDRHDGLLTSLASEGWMMERADGSTVSVSPKPRFVADESRVLREAALAGLGITTL
CCCCHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCCEECCHHHHHHHHHHHCCCCCCC
PGKLCQQEIESGTLIRILPGWTAGKVVTTILMPHRRGQLPSVRATVDFIAERLVDG
CHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]