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The map label for this gene is dnaG [H]

Identifier: 150397427

GI number: 150397427

Start: 2306655

End: 2308670

Strand: Reverse

Name: dnaG [H]

Synonym: Smed_2227

Alternate gene names: 150397427

Gene position: 2308670-2306655 (Counterclockwise)

Preceding gene: 150397428

Following gene: 150397426

Centisome position: 61.05

GC content: 64.43

Gene sequence:

>2016_bases
ATGCGCTTTTCACCGTCCTTCCTTGACGAGATACGCGACCGCGTCCCCATTTCGGACGTGGTCGGTAAACGCGTCACCTG
GGATCGGCGCAAGACCAATGTTTCGCGCGGCGATTATTGGGCCTGCTGCCCCTTTCACGGCGAAAAATCGCCGAGTTTTC
ACTGCGAGGACCGCAAAGGCCGCTATCATTGCTTCGGCTGCGGCGTTTCCGGCGACCATTTCCGCTTCCTGACCGAGCTC
GAAGGCCTCAGCTTTCCGGAAGCCGTGCAGCAGATCGCCGATATGGCAGGCGTCGCGATGCCGCAGCCCGACCCGCAGGC
CGAGCAGCGCGAGCGGGAGCGCACGAGCCTGCTCGACGTCATGGAACTTGCGACGCACTTCTTTCAGGATCAGCTTCAGA
GTGCGACCGGGGCGAAGGCGCGCGCCTATCTGCGCGAGCGCGGGCTGACCGGCCGTACGATCGAGACATTCCGCCTCGGC
TTTGCACCCGACAGCCGAAACGCGCTGAAGGAGTTCCTGGCGGGCAAGGGGATCGGCAAGGAGCAGATCGAGGCCTGTGG
CCTCGTCGTTCACGGACCGGATATTCCTGTTTCCTATGATCGCTTCCGCGACAGGATCATGTTTCCTATCCTTTCCGCGC
GCGAGAAGGTGATTGCCTTTGGCGGGCGGGCCATGTCGCCGGATGCGCCGGCAAAATATCTGAATTCCAACGAGACGGAG
CTCTTTCACAAGGGCAATGTCCTCTTCAATTTCACGCGGGCACGGCGTGCCTCGCAGGGCGCCGATGGCGCAGGCACGAT
CATTGCCGTCGAAGGCTATATGGATGTGATCGCGCTTCATCAGGCCGGGATCGAAAATGCCGTCGCGCCGCTCGGGACCG
CGCTTACCGAGAACCAGCTCGATCTGTTGTGGAAGCTTACCGCCCAGCCGGTGCTCTGCTTCGATGGCGACGGCGCCGGT
ATCCGCGCCGCCCACCGGGCTGTCGATCTCGCATTGCCCCATCTGAAACCGGGCCGTTCCGTGCGCTTTGCCATGCTGCC
GGAGGGCAAGGACCCCGACGATGTGGTGCGCCAGGATGGACGCGAGCCCTTCGACAGGGTGCTCGCCAATGCCCGCTCGC
TCGCCGACATGGTCTGGCTTCGCGAAGTGCAGGGCGGCGATTTCGACACACCGGAAAAGCGCGCCGAGCTCGAAGGGCGG
CTGCGGCAGGTGAGCGCGGTCATCGCCGACGAGAGTGTGCGCCGCCACTACGGGCAGGACATGCGCGACCGCCTGAACGC
GTTTCTTCAGGGGAGCGCGCCTTTCAGAGGCGAGAGGCGGCCGTTCGATCGGGGCGGAAGGCAGGGCGGAGGCCAGGCCG
GACGCCAGGGCGGCAGGGGCTGGTCGCGAGCTGCGAACCCGGTCCTGGGCGCGGCCGTGGAGGCCGGCACTGCCGGCAGT
TCCAAACTCAACCAGATGATGAAGGCCGGCGGCTCGCTTCGACCGCCGGTGCTGCGCGAGAGCGTGCTCGCATTGACGAT
CGTCAATCATCCGCAACTGCTATTCGACGAATATGACGAGATCGCCACGATCGAGTTCGACCACCGTGACCTGCAGCGCT
GCTGGGCCGCGGTTCTGAACGCCGCGGCAGCGAACGGGTTGCGGCTGACGCGGGAAACCCTCCTGGAGCAGCTTGAGGCG
GAGGGTTTTTCCCAGTTGATCGGCGCTCTCGACCAGCAGATTAGAAATGCGCGGCTTTGGACGGCAACTGAGGCAGCGGC
GCCGGAGGATGCACGCGAAGGCTATCTCCAGGCCCTGACCCTTCACCGGCGGACGAAGGCGCTGCTTTGGCAGAAACGGG
AACTCGAACGCGAAATCGCAGAAGCCACGGCCTCCGAAGACGCCGATCAAGGGCAGCGGCTCGTGCGCGCCATGGAGGAA
GTACAGCTGGAGCTTCACCGGATGGACAAGCTCGAAGCAATCATCGAAGGCTTCGGCGTGCTTTCGGGGAGGGTGAAGGG
GCCTGCGGCGCGGTAA

Upstream 100 bases:

>100_bases
GCCCGTGGCTTCCTCCGGCGATGCCGCTTATATGGAGGTTCCATGCCTGCCGCCCGAAGTCGTCGCACGGGGTTCGGGCG
GCATGCAGCAGAGGTAATCC

Downstream 100 bases:

>100_bases
TCATCTGTCGTGCGGCGAGTCAGCAGGCTAGCGGAATGCTGCCTGTGGTTTACACGCGAGCACCCGCGACGCCATATTGT
ACGCACCGACGAAAGCGATT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MRFSPSFLDEIRDRVPISDVVGKRVTWDRRKTNVSRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGVAMPQPDPQAEQRERERTSLLDVMELATHFFQDQLQSATGAKARAYLRERGLTGRTIETFRLG
FAPDSRNALKEFLAGKGIGKEQIEACGLVVHGPDIPVSYDRFRDRIMFPILSAREKVIAFGGRAMSPDAPAKYLNSNETE
LFHKGNVLFNFTRARRASQGADGAGTIIAVEGYMDVIALHQAGIENAVAPLGTALTENQLDLLWKLTAQPVLCFDGDGAG
IRAAHRAVDLALPHLKPGRSVRFAMLPEGKDPDDVVRQDGREPFDRVLANARSLADMVWLREVQGGDFDTPEKRAELEGR
LRQVSAVIADESVRRHYGQDMRDRLNAFLQGSAPFRGERRPFDRGGRQGGGQAGRQGGRGWSRAANPVLGAAVEAGTAGS
SKLNQMMKAGGSLRPPVLRESVLALTIVNHPQLLFDEYDEIATIEFDHRDLQRCWAAVLNAAAANGLRLTRETLLEQLEA
EGFSQLIGALDQQIRNARLWTATEAAAPEDAREGYLQALTLHRRTKALLWQKRELEREIAEATASEDADQGQRLVRAMEE
VQLELHRMDKLEAIIEGFGVLSGRVKGPAAR

Sequences:

>Translated_671_residues
MRFSPSFLDEIRDRVPISDVVGKRVTWDRRKTNVSRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGVAMPQPDPQAEQRERERTSLLDVMELATHFFQDQLQSATGAKARAYLRERGLTGRTIETFRLG
FAPDSRNALKEFLAGKGIGKEQIEACGLVVHGPDIPVSYDRFRDRIMFPILSAREKVIAFGGRAMSPDAPAKYLNSNETE
LFHKGNVLFNFTRARRASQGADGAGTIIAVEGYMDVIALHQAGIENAVAPLGTALTENQLDLLWKLTAQPVLCFDGDGAG
IRAAHRAVDLALPHLKPGRSVRFAMLPEGKDPDDVVRQDGREPFDRVLANARSLADMVWLREVQGGDFDTPEKRAELEGR
LRQVSAVIADESVRRHYGQDMRDRLNAFLQGSAPFRGERRPFDRGGRQGGGQAGRQGGRGWSRAANPVLGAAVEAGTAGS
SKLNQMMKAGGSLRPPVLRESVLALTIVNHPQLLFDEYDEIATIEFDHRDLQRCWAAVLNAAAANGLRLTRETLLEQLEA
EGFSQLIGALDQQIRNARLWTATEAAAPEDAREGYLQALTLHRRTKALLWQKRELEREIAEATASEDADQGQRLVRAMEE
VQLELHRMDKLEAIIEGFGVLSGRVKGPAAR
>Mature_671_residues
MRFSPSFLDEIRDRVPISDVVGKRVTWDRRKTNVSRGDYWACCPFHGEKSPSFHCEDRKGRYHCFGCGVSGDHFRFLTEL
EGLSFPEAVQQIADMAGVAMPQPDPQAEQRERERTSLLDVMELATHFFQDQLQSATGAKARAYLRERGLTGRTIETFRLG
FAPDSRNALKEFLAGKGIGKEQIEACGLVVHGPDIPVSYDRFRDRIMFPILSAREKVIAFGGRAMSPDAPAKYLNSNETE
LFHKGNVLFNFTRARRASQGADGAGTIIAVEGYMDVIALHQAGIENAVAPLGTALTENQLDLLWKLTAQPVLCFDGDGAG
IRAAHRAVDLALPHLKPGRSVRFAMLPEGKDPDDVVRQDGREPFDRVLANARSLADMVWLREVQGGDFDTPEKRAELEGR
LRQVSAVIADESVRRHYGQDMRDRLNAFLQGSAPFRGERRPFDRGGRQGGGQAGRQGGRGWSRAANPVLGAAVEAGTAGS
SKLNQMMKAGGSLRPPVLRESVLALTIVNHPQLLFDEYDEIATIEFDHRDLQRCWAAVLNAAAANGLRLTRETLLEQLEA
EGFSQLIGALDQQIRNARLWTATEAAAPEDAREGYLQALTLHRRTKALLWQKRELEREIAEATASEDADQGQRLVRAMEE
VQLELHRMDKLEAIIEGFGVLSGRVKGPAAR

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=427, Percent_Identity=38.4074941451991, Blast_Score=287, Evalue=1e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 74339; Mature: 74339

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFSPSFLDEIRDRVPISDVVGKRVTWDRRKTNVSRGDYWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELEGLSFPEAVQQIADMAGVAMPQPDPQAEQRERERTSLLDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MELATHFFQDQLQSATGAKARAYLRERGLTGRTIETFRLGFAPDSRNALKEFLAGKGIGK
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHCCCCCH
EQIEACGLVVHGPDIPVSYDRFRDRIMFPILSAREKVIAFGGRAMSPDAPAKYLNSNETE
HHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCCCCCE
LFHKGNVLFNFTRARRASQGADGAGTIIAVEGYMDVIALHQAGIENAVAPLGTALTENQL
EEECCCEEEEEHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHH
DLLWKLTAQPVLCFDGDGAGIRAAHRAVDLALPHLKPGRSVRFAMLPEGKDPDDVVRQDG
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCC
REPFDRVLANARSLADMVWLREVQGGDFDTPEKRAELEGRLRQVSAVIADESVRRHYGQD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRDRLNAFLQGSAPFRGERRPFDRGGRQGGGQAGRQGGRGWSRAANPVLGAAVEAGTAGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHCCCHHHHHHHCCCCCH
SKLNQMMKAGGSLRPPVLRESVLALTIVNHPQLLFDEYDEIATIEFDHRDLQRCWAAVLN
HHHHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHCCCCEEEEEECHHHHHHHHHHHHH
AAAANGLRLTRETLLEQLEAEGFSQLIGALDQQIRNARLWTATEAAAPEDAREGYLQALT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHH
LHRRTKALLWQKRELEREIAEATASEDADQGQRLVRAMEEVQLELHRMDKLEAIIEGFGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGRVKGPAAR
HCCCCCCCCCC
>Mature Secondary Structure
MRFSPSFLDEIRDRVPISDVVGKRVTWDRRKTNVSRGDYWACCPFHGEKSPSFHCEDRKG
CCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RYHCFGCGVSGDHFRFLTELEGLSFPEAVQQIADMAGVAMPQPDPQAEQRERERTSLLDV
CEEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MELATHFFQDQLQSATGAKARAYLRERGLTGRTIETFRLGFAPDSRNALKEFLAGKGIGK
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHEEECCCCCCHHHHHHHHHCCCCCH
EQIEACGLVVHGPDIPVSYDRFRDRIMFPILSAREKVIAFGGRAMSPDAPAKYLNSNETE
HHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHCCCCCCE
LFHKGNVLFNFTRARRASQGADGAGTIIAVEGYMDVIALHQAGIENAVAPLGTALTENQL
EEECCCEEEEEHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHCHHHHHHHHHHHHCCHHH
DLLWKLTAQPVLCFDGDGAGIRAAHRAVDLALPHLKPGRSVRFAMLPEGKDPDDVVRQDG
HHHHHHCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHCC
REPFDRVLANARSLADMVWLREVQGGDFDTPEKRAELEGRLRQVSAVIADESVRRHYGQD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
MRDRLNAFLQGSAPFRGERRPFDRGGRQGGGQAGRQGGRGWSRAANPVLGAAVEAGTAGS
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCHHHHCCCHHHHHHHCCCCCH
SKLNQMMKAGGSLRPPVLRESVLALTIVNHPQLLFDEYDEIATIEFDHRDLQRCWAAVLN
HHHHHHHHCCCCCCCHHHHHHHHEEEEECCCHHHHHCCCCEEEEEECHHHHHHHHHHHHH
AAAANGLRLTRETLLEQLEAEGFSQLIGALDQQIRNARLWTATEAAAPEDAREGYLQALT
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHH
LHRRTKALLWQKRELEREIAEATASEDADQGQRLVRAMEEVQLELHRMDKLEAIIEGFGV
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGRVKGPAAR
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA