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The map label for this gene is est [H]

Identifier: 150397386

GI number: 150397386

Start: 2260768

End: 2261706

Strand: Reverse

Name: est [H]

Synonym: Smed_2185

Alternate gene names: 150397386

Gene position: 2261706-2260768 (Counterclockwise)

Preceding gene: 150397397

Following gene: 150397383

Centisome position: 59.8

GC content: 66.13

Gene sequence:

>939_bases
ATGGGATCAGGACCGACAAGACCAATGCCGACGGAAGCCGGCATTCTGGCCTTTCACGAACGCTGCGAAGCGTTCTACCC
CGCCGATGCGGTCAACGCCTCCGTCGCGCAGCAGCGCCGATGGTATGACGCACTCTGCGCCGAGTTCGATGCGCCATCGC
CGGAGGGATTGACGCGGCGTGACGACCTCGTGGCTGGTCGAATCGCCATCAGGCGCTATCGTCCGGCCGAAGTCGCGAGT
GAGACGCGTGTTTTCTACATACACGGCGGCGGCTTCGTCGTCGGCTCACTTGAGAGCCATGATGCGATCTGCGCCGAGCT
TGCCGATGGCGCGCGGGTCGAACTGGTGTCGGCGGACTATCGCCTCGCGCCCGAGCACGTATGGCCCGCAGCCTTCGAGG
ACTGCTATGACGTGATGGAAGCGCTGCTTGCCGATGGGCGTCCGCTCGTCGTCGCGGGCGACAGCGCCGGCGGCAACCTC
TGCGCTGGGATCGTGCTGAAGGCAAAAGCCGAAGGGCTCTCCGGCATCGCCGGGCAGGTCCTGATCTATCCCGGCCTCGG
CGGCGATCTGACGCGCGGCTCCTATTTGGAGATGGCCGAGGCGCCGCTGCTTTCCACCGCCGATGTGGACTATTACCGCG
ATGTGCTGAAAGCACCGGCGGATGAGCCCTTTGCGCACCCGTTGCGCGCCGCGAACCTGTCAGGGCTTCCCCCGGCCTAT
GTTTCCGGCGCCCATTTTGACCCCTTGCGTGATGATGCGCGCGCCTATGCCGCCCGGCTTGCCAAGGCGGGCGTCGACGT
CACCTATCGCGAGGAGCCGCAGATGGTCCATTCCTGGCTCCGGGCTCGCCACATGAGTCCCGGTGCGCGGGAAGGCTTCG
GCCAGCTGGTGCGGGGTGTTGCGGGACTTGCCGGCACTTGCCCCTCTCCTCGGGTCTAA

Upstream 100 bases:

>100_bases
CGAAATCAAGCAGACAAAGGTCGCCGGCAGCACTTTGAAACCTTGTACATTTGCCCTTAAATCGGCTTGATCATGGAAGC
ATGCAGCGGGGAGTGCGGGG

Downstream 100 bases:

>100_bases
GCCGAGGACTAACCCCCGCAGCCGGATGAGGGGCTTTCCCGCTCCGGTCGTTCACATGAAATCGCGCCGGATCCGCTTCT
CGAAGCTCTTCTCAAAGATC

Product: alpha/beta hydrolase domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGSGPTRPMPTEAGILAFHERCEAFYPADAVNASVAQQRRWYDALCAEFDAPSPEGLTRRDDLVAGRIAIRRYRPAEVAS
ETRVFYIHGGGFVVGSLESHDAICAELADGARVELVSADYRLAPEHVWPAAFEDCYDVMEALLADGRPLVVAGDSAGGNL
CAGIVLKAKAEGLSGIAGQVLIYPGLGGDLTRGSYLEMAEAPLLSTADVDYYRDVLKAPADEPFAHPLRAANLSGLPPAY
VSGAHFDPLRDDARAYAARLAKAGVDVTYREEPQMVHSWLRARHMSPGAREGFGQLVRGVAGLAGTCPSPRV

Sequences:

>Translated_312_residues
MGSGPTRPMPTEAGILAFHERCEAFYPADAVNASVAQQRRWYDALCAEFDAPSPEGLTRRDDLVAGRIAIRRYRPAEVAS
ETRVFYIHGGGFVVGSLESHDAICAELADGARVELVSADYRLAPEHVWPAAFEDCYDVMEALLADGRPLVVAGDSAGGNL
CAGIVLKAKAEGLSGIAGQVLIYPGLGGDLTRGSYLEMAEAPLLSTADVDYYRDVLKAPADEPFAHPLRAANLSGLPPAY
VSGAHFDPLRDDARAYAARLAKAGVDVTYREEPQMVHSWLRARHMSPGAREGFGQLVRGVAGLAGTCPSPRV
>Mature_311_residues
GSGPTRPMPTEAGILAFHERCEAFYPADAVNASVAQQRRWYDALCAEFDAPSPEGLTRRDDLVAGRIAIRRYRPAEVASE
TRVFYIHGGGFVVGSLESHDAICAELADGARVELVSADYRLAPEHVWPAAFEDCYDVMEALLADGRPLVVAGDSAGGNLC
AGIVLKAKAEGLSGIAGQVLIYPGLGGDLTRGSYLEMAEAPLLSTADVDYYRDVLKAPADEPFAHPLRAANLSGLPPAYV
SGAHFDPLRDDARAYAARLAKAGVDVTYREEPQMVHSWLRARHMSPGAREGFGQLVRGVAGLAGTCPSPRV

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Secreted (Potential). Note=External to the cytoplasmic membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=281, Percent_Identity=27.4021352313167, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI206597554, Length=307, Percent_Identity=27.6872964169381, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI61966717, Length=280, Percent_Identity=27.8571428571429, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI68051721, Length=168, Percent_Identity=30.3571428571429, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI21328446, Length=112, Percent_Identity=35.7142857142857, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI157041239, Length=162, Percent_Identity=30.2469135802469, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=278, Percent_Identity=28.4172661870504, Blast_Score=95, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI72001146, Length=281, Percent_Identity=29.5373665480427, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71996133, Length=138, Percent_Identity=36.9565217391304, Blast_Score=87, Evalue=8e-18,
Organism=Caenorhabditis elegans, GI17567059, Length=139, Percent_Identity=32.3741007194245, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540028, Length=138, Percent_Identity=34.0579710144928, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33309; Mature: 33178

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSGPTRPMPTEAGILAFHERCEAFYPADAVNASVAQQRRWYDALCAEFDAPSPEGLTRR
CCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DDLVAGRIAIRRYRPAEVASETRVFYIHGGGFVVGSLESHDAICAELADGARVELVSADY
HHHHHHHHHHHHCCCHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCEEEEEECCC
RLAPEHVWPAAFEDCYDVMEALLADGRPLVVAGDSAGGNLCAGIVLKAKAEGLSGIAGQV
EECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEHEEEEECCCCCCCCCCCE
LIYPGLGGDLTRGSYLEMAEAPLLSTADVDYYRDVLKAPADEPFAHPLRAANLSGLPPAY
EEECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHH
VSGAHFDPLRDDARAYAARLAKAGVDVTYREEPQMVHSWLRARHMSPGAREGFGQLVRGV
HCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGLAGTCPSPRV
HHHCCCCCCCCC
>Mature Secondary Structure 
GSGPTRPMPTEAGILAFHERCEAFYPADAVNASVAQQRRWYDALCAEFDAPSPEGLTRR
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
DDLVAGRIAIRRYRPAEVASETRVFYIHGGGFVVGSLESHDAICAELADGARVELVSADY
HHHHHHHHHHHHCCCHHHHCCCEEEEEECCCEEEECCCCCHHHHHHHCCCCEEEEEECCC
RLAPEHVWPAAFEDCYDVMEALLADGRPLVVAGDSAGGNLCAGIVLKAKAEGLSGIAGQV
EECCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEHEEEEECCCCCCCCCCCE
LIYPGLGGDLTRGSYLEMAEAPLLSTADVDYYRDVLKAPADEPFAHPLRAANLSGLPPAY
EEECCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHCCCCCCCHHH
VSGAHFDPLRDDARAYAARLAKAGVDVTYREEPQMVHSWLRARHMSPGAREGFGQLVRGV
HCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
AGLAGTCPSPRV
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2545531 [H]