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The map label for this gene is dppA [H]

Identifier: 150397379

GI number: 150397379

Start: 2250833

End: 2252422

Strand: Direct

Name: dppA [H]

Synonym: Smed_2178

Alternate gene names: 150397379

Gene position: 2250833-2252422 (Clockwise)

Preceding gene: 150397378

Following gene: 150397380

Centisome position: 59.52

GC content: 61.07

Gene sequence:

>1590_bases
ATGAGCGATTACAAAGACTACTTGGCTCGTCAGGTCATGCTCGGCAAGATGAAGCGGCGCGAGTTTCTCGGACGCGCAGC
CGCTCTCGGCATTGCCGCGTCGAGCGCAAATATGCTCTTCGCGTCCAGCGCCGCAGCGCAGGAGCCAAAACGCGGCGGTC
ACCTTAAGCTCGGCCTAGAAGGGGCTGCTGCAACGGACTCGCGGGACCCCGCAAAGGCCCTGTCGCAATTCATGTTCGTC
GTCGGCCGCAACTGGGGCGACATGCTGGTCGAGAGCCATCCGACGACCGGCGAGCCGGTGCCGGCACTTGCGGAATCCTG
GGAACCCTCCGCCGATGCGTCCACCTGGACCTTTACGATCCGCAAGGGCGTGAAATTCCATGACGGCAAGGAACTGACGA
TCGACGACGTCATCAAGACTCTGCAGCGACACACGGATGAAAAATCCGAGTCCGGCGCGCTCGGCGTGATGAAATCCATC
AAGGAAATCAAGGCCGATGGCGATAAGCTCGTCCTGGTGCTGACGGAAGGCAATGCGGACCTGCCGCTGCTTTTGACCGA
CTACCATTTGATCATCCAGCCGAACGGCGGCACCGACAATCCCGATGCGATGATCGGTACCGGTCCCTACAAGGTCGCAA
GCTTCGAGCCGGGCATACGCGCCACGTTCGAGAAGAACCCGGACGACTGGCGCACCGACCGCGGCTTCGTCGATTCCATC
GAATTGATCGCCATGAACGACGCGACGGCGCGTGTCGCGGCGCTTTCCTCGGGCCAGGTCCACTTCATCAACCGCGTCGA
TCCTAAAACCGTCAATCTGCTGAAGAAGGCGCCGACTGTTGAAATCCTCAACACGTCCGGCCGCGGCCACTACGTGTTCA
TCATGCATTGCAACACGGCGCCCTTCGACAACAACGATCTGCGCATGGCGCTGAAATATGCCATGGACCGCGAGACCCTC
GTAGAGCGCATCCTCGGCGGCTACGGCAAGATCGGGAACGACTTCCCGATCAACGACACCTATGCGCTTTTCCCGGAAGG
GATAGAGCAACGCACTTACGACCCCGACAAGGCCGCATTCCACTACAAGAAATCCGGCCATAGCGGTCCGGTGCTGCTGC
GCACCTCCGACGTCGCCTTCCCGAACGCGGTCGACGCCGCGGTTCTCTACCAGGCGAGCGCCAGGAAGGCCGGCATCGAG
ATCGAGGTCAAGCGCGAGCCCGGCGACGGCTACTGGTCCAATGTCTGGAACGTCCAGCCTTTCTCGACATCTTATTGGGG
CGGACGCCCGACCCAGGATCAGATGTACTCCACCGCCTATCTCTCGACGGCCGACTGGAACGACACCCGTTTCAAGCGTC
CGGATTTCGACAAGATCCTGCTTGAGGCGCGTTCCGAGCTGGACGAAGCCAAGCGCAAGGACATGTACCGCACCATGGCG
ATGATGGTGCGCGACGAGGGCGGCCTGATCCTGCCCATGTTCAACGACTTCGTGAACGCGGCCGGCAAGACGGTGAAGGG
CTATGTCCACGACATCGGCAACGACATGTCCAACGGCTATGTCGCGACCAGGGTATGGCTGGACGCCTGA

Upstream 100 bases:

>100_bases
GGACTGCAAACCACCTTCCGGCCATCGACCGATGGCAATGCGCGAACACTCTACCAATCACAACGATCGGCGAATGCCGA
CCCAGGAAGGGAACAGCGGA

Downstream 100 bases:

>100_bases
TGATGGAAAGCGGACCGATCACGGGTCCGGTCGCGACGGATGCGACCGCAGGCGAGACAGCCCTGCGGTCCTCCGATCTT
GCCGGTATCCCAGCCAAACC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MSDYKDYLARQVMLGKMKRREFLGRAAALGIAASSANMLFASSAAAQEPKRGGHLKLGLEGAAATDSRDPAKALSQFMFV
VGRNWGDMLVESHPTTGEPVPALAESWEPSADASTWTFTIRKGVKFHDGKELTIDDVIKTLQRHTDEKSESGALGVMKSI
KEIKADGDKLVLVLTEGNADLPLLLTDYHLIIQPNGGTDNPDAMIGTGPYKVASFEPGIRATFEKNPDDWRTDRGFVDSI
ELIAMNDATARVAALSSGQVHFINRVDPKTVNLLKKAPTVEILNTSGRGHYVFIMHCNTAPFDNNDLRMALKYAMDRETL
VERILGGYGKIGNDFPINDTYALFPEGIEQRTYDPDKAAFHYKKSGHSGPVLLRTSDVAFPNAVDAAVLYQASARKAGIE
IEVKREPGDGYWSNVWNVQPFSTSYWGGRPTQDQMYSTAYLSTADWNDTRFKRPDFDKILLEARSELDEAKRKDMYRTMA
MMVRDEGGLILPMFNDFVNAAGKTVKGYVHDIGNDMSNGYVATRVWLDA

Sequences:

>Translated_529_residues
MSDYKDYLARQVMLGKMKRREFLGRAAALGIAASSANMLFASSAAAQEPKRGGHLKLGLEGAAATDSRDPAKALSQFMFV
VGRNWGDMLVESHPTTGEPVPALAESWEPSADASTWTFTIRKGVKFHDGKELTIDDVIKTLQRHTDEKSESGALGVMKSI
KEIKADGDKLVLVLTEGNADLPLLLTDYHLIIQPNGGTDNPDAMIGTGPYKVASFEPGIRATFEKNPDDWRTDRGFVDSI
ELIAMNDATARVAALSSGQVHFINRVDPKTVNLLKKAPTVEILNTSGRGHYVFIMHCNTAPFDNNDLRMALKYAMDRETL
VERILGGYGKIGNDFPINDTYALFPEGIEQRTYDPDKAAFHYKKSGHSGPVLLRTSDVAFPNAVDAAVLYQASARKAGIE
IEVKREPGDGYWSNVWNVQPFSTSYWGGRPTQDQMYSTAYLSTADWNDTRFKRPDFDKILLEARSELDEAKRKDMYRTMA
MMVRDEGGLILPMFNDFVNAAGKTVKGYVHDIGNDMSNGYVATRVWLDA
>Mature_528_residues
SDYKDYLARQVMLGKMKRREFLGRAAALGIAASSANMLFASSAAAQEPKRGGHLKLGLEGAAATDSRDPAKALSQFMFVV
GRNWGDMLVESHPTTGEPVPALAESWEPSADASTWTFTIRKGVKFHDGKELTIDDVIKTLQRHTDEKSESGALGVMKSIK
EIKADGDKLVLVLTEGNADLPLLLTDYHLIIQPNGGTDNPDAMIGTGPYKVASFEPGIRATFEKNPDDWRTDRGFVDSIE
LIAMNDATARVAALSSGQVHFINRVDPKTVNLLKKAPTVEILNTSGRGHYVFIMHCNTAPFDNNDLRMALKYAMDRETLV
ERILGGYGKIGNDFPINDTYALFPEGIEQRTYDPDKAAFHYKKSGHSGPVLLRTSDVAFPNAVDAAVLYQASARKAGIEI
EVKREPGDGYWSNVWNVQPFSTSYWGGRPTQDQMYSTAYLSTADWNDTRFKRPDFDKILLEARSELDEAKRKDMYRTMAM
MVRDEGGLILPMFNDFVNAAGKTVKGYVHDIGNDMSNGYVATRVWLDA

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=521, Percent_Identity=23.6084452975048, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1787495, Length=456, Percent_Identity=24.1228070175439, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1787052, Length=430, Percent_Identity=23.2558139534884, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87081878, Length=481, Percent_Identity=23.9085239085239, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787762, Length=442, Percent_Identity=23.3031674208145, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=432, Percent_Identity=23.6111111111111, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58534; Mature: 58403

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYKDYLARQVMLGKMKRREFLGRAAALGIAASSANMLFASSAAAQEPKRGGHLKLGLE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCHHHCCCCCCCEEEEEEC
GAAATDSRDPAKALSQFMFVVGRNWGDMLVESHPTTGEPVPALAESWEPSADASTWTFTI
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCHHHHCCCCCCCCCEEEEEE
RKGVKFHDGKELTIDDVIKTLQRHTDEKSESGALGVMKSIKEIKADGDKLVLVLTEGNAD
ECCCEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
LPLLLTDYHLIIQPNGGTDNPDAMIGTGPYKVASFEPGIRATFEKNPDDWRTDRGFVDSI
CCEEEECEEEEEECCCCCCCCCCEECCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCE
ELIAMNDATARVAALSSGQVHFINRVDPKTVNLLKKAPTVEILNTSGRGHYVFIMHCNTA
EEEEECCCCEEEEEECCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCCC
PFDNNDLRMALKYAMDRETLVERILGGYGKIGNDFPINDTYALFPEGIEQRTYDPDKAAF
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCHHEE
HYKKSGHSGPVLLRTSDVAFPNAVDAAVLYQASARKAGIEIEVKREPGDGYWSNVWNVQP
EEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCCCCCC
FSTSYWGGRPTQDQMYSTAYLSTADWNDTRFKRPDFDKILLEARSELDEAKRKDMYRTMA
CCCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MMVRDEGGLILPMFNDFVNAAGKTVKGYVHDIGNDMSNGYVATRVWLDA
HHEECCCCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEECC
>Mature Secondary Structure 
SDYKDYLARQVMLGKMKRREFLGRAAALGIAASSANMLFASSAAAQEPKRGGHLKLGLE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCHHHCCCCCCCEEEEEEC
GAAATDSRDPAKALSQFMFVVGRNWGDMLVESHPTTGEPVPALAESWEPSADASTWTFTI
CCCCCCCCCHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCHHHHCCCCCCCCCEEEEEE
RKGVKFHDGKELTIDDVIKTLQRHTDEKSESGALGVMKSIKEIKADGDKLVLVLTEGNAD
ECCCEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCC
LPLLLTDYHLIIQPNGGTDNPDAMIGTGPYKVASFEPGIRATFEKNPDDWRTDRGFVDSI
CCEEEECEEEEEECCCCCCCCCCEECCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCE
ELIAMNDATARVAALSSGQVHFINRVDPKTVNLLKKAPTVEILNTSGRGHYVFIMHCNTA
EEEEECCCCEEEEEECCCCEEEEECCCCHHHHHHHCCCCEEEEECCCCCCEEEEEECCCC
PFDNNDLRMALKYAMDRETLVERILGGYGKIGNDFPINDTYALFPEGIEQRTYDPDKAAF
CCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHCCCCCCHHEE
HYKKSGHSGPVLLRTSDVAFPNAVDAAVLYQASARKAGIEIEVKREPGDGYWSNVWNVQP
EEECCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCHHHCCCCCCC
FSTSYWGGRPTQDQMYSTAYLSTADWNDTRFKRPDFDKILLEARSELDEAKRKDMYRTMA
CCCCCCCCCCCHHHHHEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MMVRDEGGLILPMFNDFVNAAGKTVKGYVHDIGNDMSNGYVATRVWLDA
HHEECCCCEEEEHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]