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The map label for this gene is caiD [H]

Identifier: 150397378

GI number: 150397378

Start: 2249712

End: 2250494

Strand: Direct

Name: caiD [H]

Synonym: Smed_2177

Alternate gene names: 150397378

Gene position: 2249712-2250494 (Clockwise)

Preceding gene: 150397377

Following gene: 150397379

Centisome position: 59.49

GC content: 63.86

Gene sequence:

>783_bases
ATGACCGGACCGATCCATACCCGCCGCGAAGGTGGCATTCTCGAAGTCACGATCGACCGGCCGAAGGCCAATGCCATCGA
TCTGAAGACCAGCCGCATCATGGGCGAGATCTTCCGCGATTTTCGGGACGACCCGGAGCTTCGCGTCGCGATCATCACAG
GCGCCGGGGAGAAGTTCTTCTGCCCGGGCTGGGACCTGAAGGCGGCGGCAGACGGCGACGCAGTCGACGGAGACTATGGC
GTCGGCGGCTTCGGCGGCATGCAGGAATTGCGTGACCTCAACAAGCCGATCATCGCGGCCGTCAACGGCATTTGTTGCGG
TGGTGGGCTGGAGATCGCACTCTCCACCGACCTCATCCTCGCAACCGAACATGCGAGTTTTGCACTCCCGGAGATCCGCT
CGGGCACGCTTGCCGATGCCGCGTCGATCAAACTGCCGAAACGCATCCCCTATCACATTGCCATGGACATGCTGCTCACC
GGCCGCTGGCTCGACGCGGTCGAGGCGAATCGCTGGGGCTTCGTCAATGAAATTCTGCCGGCCGGACGGCTGATGGACAG
AGCCTGGGAGCTGGCGCGCCTGCTCGAAAGCGGACCGCCGCTCGTCTATGCCGCGATCAAGGAAGTCGTCCGTGATGCGG
AAGGCCGGGACTTCCAGACCACGATGAACCGGGTCACCCGCCGCCAGTTCAAGACGGTCGACACCCTCTATTCGAGCGAA
GACCAGCTGGAGGGCGCCCGCGCCTTCGCGGAAAAGCGCGACCCGGTCTGGAAGGGACGCTGA

Upstream 100 bases:

>100_bases
TCCAAGCTCGAAGAACTCGATATCAATCCTGTTATGGTGTTGCCGGAGGGCCAGGGAGCCCTTGCAGCCGACGCCCTGAT
CCGCCGGAGGAAATGACCCT

Downstream 100 bases:

>100_bases
GACAAGCGGTCATCGGCCGCAAACTGGAGAGGAGCCGGATAATACCGGCCGTTCAGCGTGGCGCGCCGCGAACACCGGTG
TGTGCGGATGATCAGTCATT

Product: carnitinyl-CoA dehydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTGPIHTRREGGILEVTIDRPKANAIDLKTSRIMGEIFRDFRDDPELRVAIITGAGEKFFCPGWDLKAAADGDAVDGDYG
VGGFGGMQELRDLNKPIIAAVNGICCGGGLEIALSTDLILATEHASFALPEIRSGTLADAASIKLPKRIPYHIAMDMLLT
GRWLDAVEANRWGFVNEILPAGRLMDRAWELARLLESGPPLVYAAIKEVVRDAEGRDFQTTMNRVTRRQFKTVDTLYSSE
DQLEGARAFAEKRDPVWKGR

Sequences:

>Translated_260_residues
MTGPIHTRREGGILEVTIDRPKANAIDLKTSRIMGEIFRDFRDDPELRVAIITGAGEKFFCPGWDLKAAADGDAVDGDYG
VGGFGGMQELRDLNKPIIAAVNGICCGGGLEIALSTDLILATEHASFALPEIRSGTLADAASIKLPKRIPYHIAMDMLLT
GRWLDAVEANRWGFVNEILPAGRLMDRAWELARLLESGPPLVYAAIKEVVRDAEGRDFQTTMNRVTRRQFKTVDTLYSSE
DQLEGARAFAEKRDPVWKGR
>Mature_259_residues
TGPIHTRREGGILEVTIDRPKANAIDLKTSRIMGEIFRDFRDDPELRVAIITGAGEKFFCPGWDLKAAADGDAVDGDYGV
GGFGGMQELRDLNKPIIAAVNGICCGGGLEIALSTDLILATEHASFALPEIRSGTLADAASIKLPKRIPYHIAMDMLLTG
RWLDAVEANRWGFVNEILPAGRLMDRAWELARLLESGPPLVYAAIKEVVRDAEGRDFQTTMNRVTRRQFKTVDTLYSSED
QLEGARAFAEKRDPVWKGR

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=33.0612244897959, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI31542718, Length=260, Percent_Identity=28.4615384615385, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI20127408, Length=177, Percent_Identity=28.8135593220339, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI70995211, Length=264, Percent_Identity=28.030303030303, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI37594471, Length=174, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594469, Length=174, Percent_Identity=27.5862068965517, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68989263, Length=175, Percent_Identity=28, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI221142681, Length=261, Percent_Identity=52.8735632183908, Blast_Score=273, Evalue=6e-75,
Organism=Escherichia coli, GI1787659, Length=259, Percent_Identity=32.046332046332, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1788597, Length=257, Percent_Identity=28.0155642023346, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=28.1853281853282, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=31.1224489795918, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=29.6577946768061, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=32.7935222672065, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=31.9838056680162, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560910, Length=253, Percent_Identity=30.0395256916996, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17534483, Length=228, Percent_Identity=28.0701754385965, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=245, Percent_Identity=28.1632653061224, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=207, Percent_Identity=28.9855072463768, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=186, Percent_Identity=25.8064516129032, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=261, Percent_Identity=25.2873563218391, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=243, Percent_Identity=23.8683127572016, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=209, Percent_Identity=24.8803827751196, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=243, Percent_Identity=32.0987654320988, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24653477, Length=243, Percent_Identity=32.0987654320988, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21357171, Length=262, Percent_Identity=29.0076335877863, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24653139, Length=258, Percent_Identity=28.6821705426357, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24650670, Length=175, Percent_Identity=30.8571428571429, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920382, Length=213, Percent_Identity=28.169014084507, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921000, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583077, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24583079, Length=183, Percent_Identity=28.9617486338798, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI19922422, Length=261, Percent_Identity=20.6896551724138, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 28630; Mature: 28499

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGPIHTRREGGILEVTIDRPKANAIDLKTSRIMGEIFRDFRDDPELRVAIITGAGEKFF
CCCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEE
CPGWDLKAAADGDAVDGDYGVGGFGGMQELRDLNKPIIAAVNGICCGGGLEIALSTDLIL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCEEECCCEEEEEECCEEE
ATEHASFALPEIRSGTLADAASIKLPKRIPYHIAMDMLLTGRWLDAVEANRWGFVNEILP
EECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHH
AGRLMDRAWELARLLESGPPLVYAAIKEVVRDAEGRDFQTTMNRVTRRQFKTVDTLYSSE
HHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
DQLEGARAFAEKRDPVWKGR
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TGPIHTRREGGILEVTIDRPKANAIDLKTSRIMGEIFRDFRDDPELRVAIITGAGEKFF
CCCCCCCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEE
CPGWDLKAAADGDAVDGDYGVGGFGGMQELRDLNKPIIAAVNGICCGGGLEIALSTDLIL
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHCCEEECCCEEEEEECCEEE
ATEHASFALPEIRSGTLADAASIKLPKRIPYHIAMDMLLTGRWLDAVEANRWGFVNEILP
EECCCCCCCCCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHCCHHHHHHCCCCCCHHHHHH
AGRLMDRAWELARLLESGPPLVYAAIKEVVRDAEGRDFQTTMNRVTRRQFKTVDTLYSSE
HHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCH
DQLEGARAFAEKRDPVWKGR
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA