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The map label for this gene is ywlF [H]

Identifier: 150397350

GI number: 150397350

Start: 2218647

End: 2219084

Strand: Reverse

Name: ywlF [H]

Synonym: Smed_2149

Alternate gene names: 150397350

Gene position: 2219084-2218647 (Counterclockwise)

Preceding gene: 150397351

Following gene: 150397347

Centisome position: 58.68

GC content: 61.19

Gene sequence:

>438_bases
ATGAAAATCGCGATTGGAGCCGACAGCGCCGGTAAGCCCCTGCTCGACGTCATCGCCGCGCATCTCGCCACGAAAAGCGG
CCTCGAAGTGAAGGACCTGAGCCAGTCCGGCTATTATGCCGATCTCTCCCGAGATCTGGCGCAGACGATCACGGCAGGCG
AAAACGAGCGTGGCATCCTGATCTGCGGTACCGGCATTGGCGTATGCATCTCCGCCAACAAGGTGCCCGGCATCCGCGCC
GCGCTTACCCACGACACCTATTCGGCCGAGCGGGCAGCCAAGTCCAATAATGCCCAGATCATCACCATGGGCGCACGCGT
TATCGGCCCGGAATTGGCGAAATCGATCGTCGACACCTGGCTTGCCTCCGAATTCGACCCGAACGGGCCGTCTGCGGCGA
ATGTCGACGCGATCGATCGTCTGGATGCGGTGAAGTAA

Upstream 100 bases:

>100_bases
TCCGCCTGGGAGGTTGGCGGCTATCTCGACATCTTGCAACGCGTAGCCCGGGCGATCTGAGCATCGACGGGTCAGGCATC
AACATCTGAAAGGAAACGAC

Downstream 100 bases:

>100_bases
CAGTCCCGAGTTCTGCGGCGCATGCTGATCCTCCCTCATGCGCGCAGCGAGACGCCCGGCAGCAATGCCGGGTGTTTTCG
TTGTGGTGCGCCCGGCATGG

Product: ribose-5-phosphate isomerase B

Products: D-ribulose 5-phosphate

Alternate protein names: NA

Number of amino acids: Translated: 145; Mature: 145

Protein sequence:

>145_residues
MKIAIGADSAGKPLLDVIAAHLATKSGLEVKDLSQSGYYADLSRDLAQTITAGENERGILICGTGIGVCISANKVPGIRA
ALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTWLASEFDPNGPSAANVDAIDRLDAVK

Sequences:

>Translated_145_residues
MKIAIGADSAGKPLLDVIAAHLATKSGLEVKDLSQSGYYADLSRDLAQTITAGENERGILICGTGIGVCISANKVPGIRA
ALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTWLASEFDPNGPSAANVDAIDRLDAVK
>Mature_145_residues
MKIAIGADSAGKPLLDVIAAHLATKSGLEVKDLSQSGYYADLSRDLAQTITAGENERGILICGTGIGVCISANKVPGIRA
ALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTWLASEFDPNGPSAANVDAIDRLDAVK

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=145, Percent_Identity=40, Blast_Score=102, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6

Molecular weight: Translated: 15020; Mature: 15020

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIGADSAGKPLLDVIAAHLATKSGLEVKDLSQSGYYADLSRDLAQTITAGENERGIL
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCEEE
ICGTGIGVCISANKVPGIRAALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTW
EEECCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCHHHCHHHHHHHHHHH
LASEFDPNGPSAANVDAIDRLDAVK
HHCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MKIAIGADSAGKPLLDVIAAHLATKSGLEVKDLSQSGYYADLSRDLAQTITAGENERGIL
CEEEECCCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHHHHHHHHHCCCCCCEEE
ICGTGIGVCISANKVPGIRAALTHDTYSAERAAKSNNAQIITMGARVIGPELAKSIVDTW
EEECCCEEEEECCCCCCEEEEEECCCHHHHHHHCCCCCEEEEECCHHHCHHHHHHHHHHH
LASEFDPNGPSAANVDAIDRLDAVK
HHCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: D-ribose 5-phosphate

Specific reaction: D-ribose 5-phosphate = D-ribulose 5-phosphate

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]