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The map label for this gene is tpiA [H]

Identifier: 150397351

GI number: 150397351

Start: 2219125

End: 2219892

Strand: Reverse

Name: tpiA [H]

Synonym: Smed_2150

Alternate gene names: 150397351

Gene position: 2219892-2219125 (Counterclockwise)

Preceding gene: 150397352

Following gene: 150397350

Centisome position: 58.7

GC content: 63.8

Gene sequence:

>768_bases
ATGAGCAAGTCGGGGCTCTGGGTCGGCACGAGCTGGAAGATGAACAAGACGTTGGCGGAGGCCATGGTCTTCGCCGATGG
TCTTGCGGCCGCAGACGCGACGCGCGACGCGCGCATTCAACGCTTCGTCGTCCCTCCGTTCACGGCCGTACGACAAGTCA
AGGAAAGGCTGAACGGAACCAGCGTCAAAGTCGGTGCGCAGAACATGCATTGGGACGATGCGGGAGCCTGGACCGGCGAA
ATTTCACCGATGATGCTCAAGGACTGCGGTCTCGATATCGTCGAGCTTGGTCACAGCGAACGGCGCGAGCATTTCGGCGA
AACCGACCGAACCGTGGGCCTGAAAACCGCGGCCGCCGTGAAGCACGGTCTGGTGCCGTTGATCTGCATCGGTGAGACGC
TCGCCGAACGCGAGGCAGGTGAAGCGGACACGGTGTTGAGGCGCCAGGTGGAAGGCGCCCTTGCATTCCTCGGGGGCGCG
GCAAGGACGGCCCCTGTTCTCCTGGCTTACGAGCCGGTCTGGGCGATCGGCGTCAACGGCATACCGGCGACAGCCGGCTA
CGCCGACGCCCGCCACAAGATCATTGCCGACGTCGCTCAGCGTGCGCTCGGGGTGAAGGTTCCGGTGCTCTATGGCGGCA
GTGTCAATCCCGGAAATTGCGAGGAACTGATCGCTGAGCCGCACATCGACGGGCTCTTCATCGGCCGCTCCGCCTGGGAG
GTTGGCGGCTATCTCGACATCTTGCAACGCGTAGCCCGGGCGATCTGA

Upstream 100 bases:

>100_bases
TTCTGACCGGGAGCGAAGTGTCCGACGGGCACGCAAGGGAACTGGGAGACGCCGGCGTCGGGCTCCTCAGGATCGACAAC
CAGAAAATAATGGAGAAGGC

Downstream 100 bases:

>100_bases
GCATCGACGGGTCAGGCATCAACATCTGAAAGGAAACGACATGAAAATCGCGATTGGAGCCGACAGCGCCGGTAAGCCCC
TGCTCGACGTCATCGCCGCG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM 2; Triose-phosphate isomerase 2 [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSKSGLWVGTSWKMNKTLAEAMVFADGLAAADATRDARIQRFVVPPFTAVRQVKERLNGTSVKVGAQNMHWDDAGAWTGE
ISPMMLKDCGLDIVELGHSERREHFGETDRTVGLKTAAAVKHGLVPLICIGETLAEREAGEADTVLRRQVEGALAFLGGA
ARTAPVLLAYEPVWAIGVNGIPATAGYADARHKIIADVAQRALGVKVPVLYGGSVNPGNCEELIAEPHIDGLFIGRSAWE
VGGYLDILQRVARAI

Sequences:

>Translated_255_residues
MSKSGLWVGTSWKMNKTLAEAMVFADGLAAADATRDARIQRFVVPPFTAVRQVKERLNGTSVKVGAQNMHWDDAGAWTGE
ISPMMLKDCGLDIVELGHSERREHFGETDRTVGLKTAAAVKHGLVPLICIGETLAEREAGEADTVLRRQVEGALAFLGGA
ARTAPVLLAYEPVWAIGVNGIPATAGYADARHKIIADVAQRALGVKVPVLYGGSVNPGNCEELIAEPHIDGLFIGRSAWE
VGGYLDILQRVARAI
>Mature_254_residues
SKSGLWVGTSWKMNKTLAEAMVFADGLAAADATRDARIQRFVVPPFTAVRQVKERLNGTSVKVGAQNMHWDDAGAWTGEI
SPMMLKDCGLDIVELGHSERREHFGETDRTVGLKTAAAVKHGLVPLICIGETLAEREAGEADTVLRRQVEGALAFLGGAA
RTAPVLLAYEPVWAIGVNGIPATAGYADARHKIIADVAQRALGVKVPVLYGGSVNPGNCEELIAEPHIDGLFIGRSAWEV
GGYLDILQRVARAI

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4507645, Length=234, Percent_Identity=38.034188034188, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI226529917, Length=235, Percent_Identity=38.2978723404255, Blast_Score=139, Evalue=3e-33,
Organism=Escherichia coli, GI1790353, Length=210, Percent_Identity=40.4761904761905, Blast_Score=150, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17536593, Length=239, Percent_Identity=38.4937238493724, Blast_Score=135, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6320255, Length=234, Percent_Identity=35.042735042735, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28572008, Length=235, Percent_Identity=38.7234042553192, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28572006, Length=235, Percent_Identity=38.7234042553192, Blast_Score=130, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28572004, Length=235, Percent_Identity=38.7234042553192, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861 [H]

Pfam domain/function: PF00121 TIM [H]

EC number: =5.3.1.1 [H]

Molecular weight: Translated: 27264; Mature: 27132

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00171 TIM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSGLWVGTSWKMNKTLAEAMVFADGLAAADATRDARIQRFVVPPFTAVRQVKERLNGT
CCCCCEEEECCCCHHHHHHHHHHHHHCHHHHCCCHHHHHEEEECCCHHHHHHHHHHHCCC
SVKVGAQNMHWDDAGAWTGEISPMMLKDCGLDIVELGHSERREHFGETDRTVGLKTAAAV
EEEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHCCCCCCCCCHHHHHHH
KHGLVPLICIGETLAEREAGEADTVLRRQVEGALAFLGGAARTAPVLLAYEPVWAIGVNG
HHCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCC
IPATAGYADARHKIIADVAQRALGVKVPVLYGGSVNPGNCEELIAEPHIDGLFIGRSAWE
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCEEEECCHHHH
VGGYLDILQRVARAI
HCCHHHHHHHHHHHC
>Mature Secondary Structure 
SKSGLWVGTSWKMNKTLAEAMVFADGLAAADATRDARIQRFVVPPFTAVRQVKERLNGT
CCCCEEEECCCCHHHHHHHHHHHHHCHHHHCCCHHHHHEEEECCCHHHHHHHHHHHCCC
SVKVGAQNMHWDDAGAWTGEISPMMLKDCGLDIVELGHSERREHFGETDRTVGLKTAAAV
EEEECCCCCCCCCCCCCCCCCCHHHHHHCCCHHHHCCCHHHHHHCCCCCCCCCHHHHHHH
KHGLVPLICIGETLAEREAGEADTVLRRQVEGALAFLGGAARTAPVLLAYEPVWAIGVNG
HHCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEECCC
IPATAGYADARHKIIADVAQRALGVKVPVLYGGSVNPGNCEELIAEPHIDGLFIGRSAWE
CCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHCCCCCEEEECCHHHH
VGGYLDILQRVARAI
HCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]