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The map label for this gene is rbsR [H]

Identifier: 150397348

GI number: 150397348

Start: 2216810

End: 2217832

Strand: Direct

Name: rbsR [H]

Synonym: Smed_2147

Alternate gene names: 150397348

Gene position: 2216810-2217832 (Clockwise)

Preceding gene: 150397342

Following gene: 150397349

Centisome position: 58.62

GC content: 58.36

Gene sequence:

>1023_bases
ATGCGAAAGATCTTCCATCGCCGACGCACTCTGCGGGACGTCGCGCTGGCGGCGGGAGTGAGCATCTCGACCGCTTCAAA
CGCCCTTAACGAGACTGGCCGCGTCAACATCGAAACCAGGGACAAGGTCAAGCGGATCGCCCAGGAGATCGGTTTCCTTC
CCAATGCGCTCGCCCGAAGCCTGCTCAGCAAGCGAAGCTTCACCATTGGCATGATCACAAACGACACCTATGGCAGGCTT
ACATTGCCTATGGCAGCGGGTGTTTCCGAAGCCCTCGTCGATCACGGCATTTCCGTTTTCCTGTGTGCGACAAACAACGA
TCAAAGGCTGGCGACGTCTCATCTGCAGGCTCTTCTGGAGAAGCAGGTCGACGGTATCATCTTCACCGCGACGCGACTGG
ATCTGGAGCCGCCTGTCGAATTGCTCAATCTGCCTATCCCTGTCGTCTATGTTTTCGCGCAGGGGCCAAATCAGAATGTC
AGTTTCGTCCCGGACGATGCCCAAGGCGCCCGTCTGGCGGTGACTCACCTGAAGCGTCTCGGCTACTCCCGCATCGTTCA
TATCACCGGCCCGCAGGACTATCTGGCAGCACGGATCAGGGCGGAGACTTATCGTGAGAGCTGTGACCGGAGCCCGGAAG
TATTTTTTGGCGACTGGAGCGAGGATTGGGGATATGTGGCGACGGAGAAAGTGTTTTCACAAGGAGGCAAACAGCCCGAC
GCCATTTTTTGCGGGAGCGACGAGATCGCCCGCGGCGTGATCGACGCTCTGCGTGACCTGGATCTATCGGTCCCCAACGA
CGTTGCTGTCGTGGGCTTTGACAACTGGGAGGTTATTTCACGCCAGACGCGGCCGCCGCTCACGACGGTCGACATGGAAC
TGAAAGAACTCGGCCGCCAGGCCGGATTGGCTATCCTTGCCCTTACGCGCGATGAAGCCATCGACACTGGCATCCAGAGG
CTTTCCTGTTCTCTGGTGGTAAGAGAGTCATGCGGAGCAGAGGGTTCCCATCCCCAGGTATGA

Upstream 100 bases:

>100_bases
GCGATTGTCGATAATTTGCGTTTCCCAACGTGCAAAAACCTTTTTGCATGAGCGATTTCAACTGGTAAAAGGTTCTTGCA
CATGAAACGGGAATCCAGGG

Downstream 100 bases:

>100_bases
AGCACCGAGCGGAATCCGGGCGGGACGCCGATGGGCACGAGAGACCGGCAAAGCAAACAATCCCAGGATAACTGGGCGGC
TGTGCTTAGATCTTCTATTC

Product: periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRKIFHRRRTLRDVALAAGVSISTASNALNETGRVNIETRDKVKRIAQEIGFLPNALARSLLSKRSFTIGMITNDTYGRL
TLPMAAGVSEALVDHGISVFLCATNNDQRLATSHLQALLEKQVDGIIFTATRLDLEPPVELLNLPIPVVYVFAQGPNQNV
SFVPDDAQGARLAVTHLKRLGYSRIVHITGPQDYLAARIRAETYRESCDRSPEVFFGDWSEDWGYVATEKVFSQGGKQPD
AIFCGSDEIARGVIDALRDLDLSVPNDVAVVGFDNWEVISRQTRPPLTTVDMELKELGRQAGLAILALTRDEAIDTGIQR
LSCSLVVRESCGAEGSHPQV

Sequences:

>Translated_340_residues
MRKIFHRRRTLRDVALAAGVSISTASNALNETGRVNIETRDKVKRIAQEIGFLPNALARSLLSKRSFTIGMITNDTYGRL
TLPMAAGVSEALVDHGISVFLCATNNDQRLATSHLQALLEKQVDGIIFTATRLDLEPPVELLNLPIPVVYVFAQGPNQNV
SFVPDDAQGARLAVTHLKRLGYSRIVHITGPQDYLAARIRAETYRESCDRSPEVFFGDWSEDWGYVATEKVFSQGGKQPD
AIFCGSDEIARGVIDALRDLDLSVPNDVAVVGFDNWEVISRQTRPPLTTVDMELKELGRQAGLAILALTRDEAIDTGIQR
LSCSLVVRESCGAEGSHPQV
>Mature_340_residues
MRKIFHRRRTLRDVALAAGVSISTASNALNETGRVNIETRDKVKRIAQEIGFLPNALARSLLSKRSFTIGMITNDTYGRL
TLPMAAGVSEALVDHGISVFLCATNNDQRLATSHLQALLEKQVDGIIFTATRLDLEPPVELLNLPIPVVYVFAQGPNQNV
SFVPDDAQGARLAVTHLKRLGYSRIVHITGPQDYLAARIRAETYRESCDRSPEVFFGDWSEDWGYVATEKVFSQGGKQPD
AIFCGSDEIARGVIDALRDLDLSVPNDVAVVGFDNWEVISRQTRPPLTTVDMELKELGRQAGLAILALTRDEAIDTGIQR
LSCSLVVRESCGAEGSHPQV

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=301, Percent_Identity=34.21926910299, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=30.2395209580838, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1786540, Length=330, Percent_Identity=30.3030303030303, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787948, Length=303, Percent_Identity=29.042904290429, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787906, Length=217, Percent_Identity=32.258064516129, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1789202, Length=346, Percent_Identity=25.7225433526012, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=25.2252252252252, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789068, Length=301, Percent_Identity=26.9102990033223, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1788474, Length=299, Percent_Identity=25.0836120401338, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787580, Length=305, Percent_Identity=28.1967213114754, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=23.8670694864048, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789456, Length=332, Percent_Identity=24.3975903614458, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786268, Length=327, Percent_Identity=22.6299694189602, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1790689, Length=312, Percent_Identity=22.1153846153846, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37327; Mature: 37327

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKIFHRRRTLRDVALAAGVSISTASNALNETGRVNIETRDKVKRIAQEIGFLPNALARS
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHH
LLSKRSFTIGMITNDTYGRLTLPMAAGVSEALVDHGISVFLCATNNDQRLATSHLQALLE
HHHCCCEEEEEEECCCCCEEEEHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KQVDGIIFTATRLDLEPPVELLNLPIPVVYVFAQGPNQNVSFVPDDAQGARLAVTHLKRL
HHCCCEEEEEEECCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHC
GYSRIVHITGPQDYLAARIRAETYRESCDRSPEVFFGDWSEDWGYVATEKVFSQGGKQPD
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCC
AIFCGSDEIARGVIDALRDLDLSVPNDVAVVGFDNWEVISRQTRPPLTTVDMELKELGRQ
EEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCEEECHHHHHHHHH
AGLAILALTRDEAIDTGIQRLSCSLVVRESCGAEGSHPQV
CCEEEEEEECHHHHHHHHHHHHHHEEEEHHCCCCCCCCCC
>Mature Secondary Structure
MRKIFHRRRTLRDVALAAGVSISTASNALNETGRVNIETRDKVKRIAQEIGFLPNALARS
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHCCCCEEEEEHHHHHHHHHHHHCCCHHHHHHH
LLSKRSFTIGMITNDTYGRLTLPMAAGVSEALVDHGISVFLCATNNDQRLATSHLQALLE
HHHCCCEEEEEEECCCCCEEEEHHHCCHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
KQVDGIIFTATRLDLEPPVELLNLPIPVVYVFAQGPNQNVSFVPDDAQGARLAVTHLKRL
HHCCCEEEEEEECCCCCCHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHC
GYSRIVHITGPQDYLAARIRAETYRESCDRSPEVFFGDWSEDWGYVATEKVFSQGGKQPD
CCEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCCCCC
AIFCGSDEIARGVIDALRDLDLSVPNDVAVVGFDNWEVISRQTRPPLTTVDMELKELGRQ
EEEECCHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCCCCCEEECHHHHHHHHH
AGLAILALTRDEAIDTGIQRLSCSLVVRESCGAEGSHPQV
CCEEEEEEECHHHHHHHHHHHHHHEEEEHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]