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The map label for this gene is folD2

Identifier: 150397296

GI number: 150397296

Start: 2155208

End: 2156101

Strand: Reverse

Name: folD2

Synonym: Smed_2095

Alternate gene names: 150397296

Gene position: 2156101-2155208 (Counterclockwise)

Preceding gene: 150397297

Following gene: 150397295

Centisome position: 57.01

GC content: 62.64

Gene sequence:

>894_bases
ATGGGAATGGATAGAATCGCCGATCGTGCGATCATTGACGGCAAACGGTTCGCCGCCGGACTGCTGGACGATATCACGGC
CAGGGTGAAGAAACTGGAGGAAGAGACCGGGCAGGTTCCCGGCCTTGCCGTCGTGCTCGTCGGCGAAGACCCTGCAAGCA
GAGTCTATGTCGGGTCGAAGCATCGCCAGACCCTTGCCGCCGGCATCGCTTCATTCAAATACGAGCTTCCGGCAGAAACC
ACGCAGGAAGAACTGATGGCGCTGATCGACCGGCTGAACGTCGATCCCGCCGTACACGGCATCCTCGTTCAATTGCCTCT
GCCGGCACATCTCGACGCCAACGCCGTCATTCAGGAGATCAACCCGAACAAAGACGTCGACGGCTTTCACATTTCCAATG
CCGGACGCCTTGCGACCGGCCAACCGGCATTCGTGCCCTGCACACCGCTCGGCTGCCTTTTACTGCTGAAAGACCGCCTC
GGCGCGAACCTGTCGGGGCTCCACGCGGTCGTTCTGGGCAAGTCCAACATAGTCGGCCGGCCGATGGTGCAACTGCTGCT
TGCCGAAAGCTGCACGGTGACGATCGCGCATTCGCGCACGCGCAACGCGCCGGAGCTTTGTCGGGAAGCGGATATCCTCA
TCGTCGCGGTGGGACGGCCGGGCCTGGTACGTGGCGACTGGATCAAGCCCGGGGCCGTGGTCATCGATGTCGGGATTAAC
CGCGTCGAAGCGGAGGGCAAGTCGCGCATCGTCGGCGACGTTGCCTTCGAGGAAGCCGGCCATGCCGGTGCCATTACGCC
CGTGCCCGGCGGGGTCGGCCCCATGACAATCGCCTGCCTGCTGTCGAATACCCTGACGGCCTTCTGCCGTCAGCACGCCG
TCAATATCGATTGA

Upstream 100 bases:

>100_bases
GGAGCGGGATGAGGAAAAGTGTGCTCGGTTGTCCGCCCGCATCCCGCTCTAACTTGCTGCAGCCGGTGCGCCCGGTCCGT
TGAACGCGAAAGGAATCCCC

Downstream 100 bases:

>100_bases
GCGGCCCGAAAGGATCGTCCGATGCCCGTCCCCTCCAATCGCTATGCATTGCGTGTCGCCTGCCCGTCCATCCGGGGGGT
GACTGCTGCCATCGCGACCT

Product: methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MGMDRIADRAIIDGKRFAAGLLDDITARVKKLEEETGQVPGLAVVLVGEDPASRVYVGSKHRQTLAAGIASFKYELPAET
TQEELMALIDRLNVDPAVHGILVQLPLPAHLDANAVIQEINPNKDVDGFHISNAGRLATGQPAFVPCTPLGCLLLLKDRL
GANLSGLHAVVLGKSNIVGRPMVQLLLAESCTVTIAHSRTRNAPELCREADILIVAVGRPGLVRGDWIKPGAVVIDVGIN
RVEAEGKSRIVGDVAFEEAGHAGAITPVPGGVGPMTIACLLSNTLTAFCRQHAVNID

Sequences:

>Translated_297_residues
MGMDRIADRAIIDGKRFAAGLLDDITARVKKLEEETGQVPGLAVVLVGEDPASRVYVGSKHRQTLAAGIASFKYELPAET
TQEELMALIDRLNVDPAVHGILVQLPLPAHLDANAVIQEINPNKDVDGFHISNAGRLATGQPAFVPCTPLGCLLLLKDRL
GANLSGLHAVVLGKSNIVGRPMVQLLLAESCTVTIAHSRTRNAPELCREADILIVAVGRPGLVRGDWIKPGAVVIDVGIN
RVEAEGKSRIVGDVAFEEAGHAGAITPVPGGVGPMTIACLLSNTLTAFCRQHAVNID
>Mature_296_residues
GMDRIADRAIIDGKRFAAGLLDDITARVKKLEEETGQVPGLAVVLVGEDPASRVYVGSKHRQTLAAGIASFKYELPAETT
QEELMALIDRLNVDPAVHGILVQLPLPAHLDANAVIQEINPNKDVDGFHISNAGRLATGQPAFVPCTPLGCLLLLKDRLG
ANLSGLHAVVLGKSNIVGRPMVQLLLAESCTVTIAHSRTRNAPELCREADILIVAVGRPGLVRGDWIKPGAVVIDVGINR
VEAEGKSRIVGDVAFEEAGHAGAITPVPGGVGPMTIACLLSNTLTAFCRQHAVNID

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=45.2380952380952, Blast_Score=229, Evalue=2e-60,
Organism=Homo sapiens, GI222418558, Length=290, Percent_Identity=45.1724137931035, Blast_Score=206, Evalue=1e-53,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=43.298969072165, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI36796743, Length=201, Percent_Identity=27.8606965174129, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=51.5901060070671, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=45.6747404844291, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6319558, Length=288, Percent_Identity=44.0972222222222, Blast_Score=232, Evalue=5e-62,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=43.1438127090301, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.6190476190476, Blast_Score=234, Evalue=5e-62,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.6190476190476, Blast_Score=234, Evalue=7e-62,
Organism=Drosophila melanogaster, GI62472483, Length=294, Percent_Identity=44.5578231292517, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI45551871, Length=294, Percent_Identity=44.5578231292517, Blast_Score=228, Evalue=4e-60,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=45.4861111111111, Blast_Score=227, Evalue=7e-60,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=45.4861111111111, Blast_Score=227, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD2_SINMW (A6UB98)

Other databases:

- EMBL:   CP000738
- RefSeq:   YP_001327763.1
- ProteinModelPortal:   A6UB98
- SMR:   A6UB98
- STRING:   A6UB98
- GeneID:   5322955
- GenomeReviews:   CP000738_GR
- KEGG:   smd:Smed_2095
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   LLEHYHI
- ProtClustDB:   CLSK2327460
- BioCyc:   SMED366394:SMED_2095-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 31346; Mature: 31215

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMDRIADRAIIDGKRFAAGLLDDITARVKKLEEETGQVPGLAVVLVGEDPASRVYVGSK
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCH
HRQTLAAGIASFKYELPAETTQEELMALIDRLNVDPAVHGILVQLPLPAHLDANAVIQEI
HHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCHHHCEEEEEECCCCCCCHHHHHEEC
NPNKDVDGFHISNAGRLATGQPAFVPCTPLGCLLLLKDRLGANLSGLHAVVLGKSNIVGR
CCCCCCCCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCH
PMVQLLLAESCTVTIAHSRTRNAPELCREADILIVAVGRPGLVRGDWIKPGAVVIDVGIN
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCCCEECCCCCCCCEEEEECCC
RVEAEGKSRIVGDVAFEEAGHAGAITPVPGGVGPMTIACLLSNTLTAFCRQHAVNID
EEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
GMDRIADRAIIDGKRFAAGLLDDITARVKKLEEETGQVPGLAVVLVGEDPASRVYVGSK
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEECCH
HRQTLAAGIASFKYELPAETTQEELMALIDRLNVDPAVHGILVQLPLPAHLDANAVIQEI
HHHHHHHHHHHHEEECCCCCCHHHHHHHHHHCCCCHHHCEEEEEECCCCCCCHHHHHEEC
NPNKDVDGFHISNAGRLATGQPAFVPCTPLGCLLLLKDRLGANLSGLHAVVLGKSNIVGR
CCCCCCCCEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCH
PMVQLLLAESCTVTIAHSRTRNAPELCREADILIVAVGRPGLVRGDWIKPGAVVIDVGIN
HHHHHHHHCCCEEEEECCCCCCCHHHHCCCCEEEEECCCCCCEECCCCCCCCEEEEECCC
RVEAEGKSRIVGDVAFEEAGHAGAITPVPGGVGPMTIACLLSNTLTAFCRQHAVNID
EEECCCCCEEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA