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The map label for this gene is purU [H]

Identifier: 150397295

GI number: 150397295

Start: 2154290

End: 2155186

Strand: Reverse

Name: purU [H]

Synonym: Smed_2094

Alternate gene names: 150397295

Gene position: 2155186-2154290 (Counterclockwise)

Preceding gene: 150397296

Following gene: 150397294

Centisome position: 56.99

GC content: 59.75

Gene sequence:

>897_bases
ATGCCCGTCCCCTCCAATCGCTATGCATTGCGTGTCGCCTGCCCGTCCATCCGGGGGGTGACTGCTGCCATCGCGACCTA
TCTATCCAACAACGGCTGCAATATCTCCGACAGCGCCCAATTCGACGACAAAAGCACCGGGCGGTATTTCATGCGCGTGA
GCTTCCAGCCGGAGGAAGGTCACACCCTCGAGCTTTTGCGCGAAGGCTTCGCCGACATCGCCGCGGAATACGAAGCGGAT
GCCGAACTCTTCGACGAGACGGCAAAGCGCAAGGTTATCCTCATGGTGTCGCGCTTCGGTCATTGCCTCAACGACCTGCT
CTACCGCTGGCGCATTGGCGCCCTGCCGATCGACATCGTGGGCGTCATCTCCAACCATTTCGACTATCAGAAGATCGTCG
TGAACCATGACATTCCCTTCCACCACATCAAGGTCACCAGGGAAAACAAGCTGGCGGCGGAAGCCGAGCAGATGCGCATA
GTGGATGAGACGGGTGCGGAGCTCATCGTGCTCGCGCGCTATATGCAGGTCCTCTCCGACGGGATGTGCAGGAAGATGTC
AGGACGCATCATCAATATCCACCATTCGTTCCTGCCGTCCTTCAAGGGCGCGAACCCTTATAAGCAGGCTTTCGAGCGGG
GCGTTAAATTGATCGGCGCGACATCGCACTACGTGACCGCGGACCTGGATGAAGGCCCCATCATCGAGCAGGAGACCGTC
CGGGTGACGCACGCCCAGAGCGCCGACGATTATGTAAGCCTCGGACGCGACGTGGAAAGCCAGGTTCTGGCGCGGGCGAT
CCATGCGCATATCCATGGCCGCGTTTTCCTGAACGGCAACAAGACCATCGTCTTCCCGCCGTCGCCGGGCTCCTTTGCAT
CCGAGCGCATGGGCTGA

Upstream 100 bases:

>100_bases
CGGCCCCATGACAATCGCCTGCCTGCTGTCGAATACCCTGACGGCCTTCTGCCGTCAGCACGCCGTCAATATCGATTGAG
CGGCCCGAAAGGATCGTCCG

Downstream 100 bases:

>100_bases
AGCGCCCGTCGAGCCGGAGCCGCAGCGCCACAACCGTTCGCCTTGATTTCGGGTACATCATGAAAGTCATCGTTCTCGGC
GCCGGCATCATCGGCGTCAC

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MPVPSNRYALRVACPSIRGVTAAIATYLSNNGCNISDSAQFDDKSTGRYFMRVSFQPEEGHTLELLREGFADIAAEYEAD
AELFDETAKRKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHFDYQKIVVNHDIPFHHIKVTRENKLAAEAEQMRI
VDETGAELIVLARYMQVLSDGMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQETV
RVTHAQSADDYVSLGRDVESQVLARAIHAHIHGRVFLNGNKTIVFPPSPGSFASERMG

Sequences:

>Translated_298_residues
MPVPSNRYALRVACPSIRGVTAAIATYLSNNGCNISDSAQFDDKSTGRYFMRVSFQPEEGHTLELLREGFADIAAEYEAD
AELFDETAKRKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHFDYQKIVVNHDIPFHHIKVTRENKLAAEAEQMRI
VDETGAELIVLARYMQVLSDGMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQETV
RVTHAQSADDYVSLGRDVESQVLARAIHAHIHGRVFLNGNKTIVFPPSPGSFASERMG
>Mature_297_residues
PVPSNRYALRVACPSIRGVTAAIATYLSNNGCNISDSAQFDDKSTGRYFMRVSFQPEEGHTLELLREGFADIAAEYEADA
ELFDETAKRKVILMVSRFGHCLNDLLYRWRIGALPIDIVGVISNHFDYQKIVVNHDIPFHHIKVTRENKLAAEAEQMRIV
DETGAELIVLARYMQVLSDGMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQETVR
VTHAQSADDYVSLGRDVESQVLARAIHAHIHGRVFLNGNKTIVFPPSPGSFASERMG

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=213, Percent_Identity=29.5774647887324, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI209869995, Length=213, Percent_Identity=29.5774647887324, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI209869993, Length=213, Percent_Identity=29.5774647887324, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787483, Length=283, Percent_Identity=39.5759717314488, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1788846, Length=208, Percent_Identity=25.9615384615385, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17567511, Length=161, Percent_Identity=32.2981366459627, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24582400, Length=198, Percent_Identity=30.8080808080808, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 33274; Mature: 33143

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVPSNRYALRVACPSIRGVTAAIATYLSNNGCNISDSAQFDDKSTGRYFMRVSFQPEEG
CCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
HTLELLREGFADIAAEYEADAELFDETAKRKVILMVSRFGHCLNDLLYRWRIGALPIDIV
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCHHHH
GVISNHFDYQKIVVNHDIPFHHIKVTRENKLAAEAEQMRIVDETGAELIVLARYMQVLSD
HHHHCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHEEHHHCCCHHHHHHHHHHHHHC
GMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQETV
CHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEE
RVTHAQSADDYVSLGRDVESQVLARAIHAHIHGRVFLNGNKTIVFPPSPGSFASERMG
EEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCCCHHHCCC
>Mature Secondary Structure 
PVPSNRYALRVACPSIRGVTAAIATYLSNNGCNISDSAQFDDKSTGRYFMRVSFQPEEG
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCC
HTLELLREGFADIAAEYEADAELFDETAKRKVILMVSRFGHCLNDLLYRWRIGALPIDIV
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHCCCCCCHHHH
GVISNHFDYQKIVVNHDIPFHHIKVTRENKLAAEAEQMRIVDETGAELIVLARYMQVLSD
HHHHCCCCEEEEEEECCCCEEEEEEECCCCHHHHHHHHEEHHHCCCHHHHHHHHHHHHHC
GMCRKMSGRIINIHHSFLPSFKGANPYKQAFERGVKLIGATSHYVTADLDEGPIIEQETV
CHHHHHCCCEEEEEHHHCCCCCCCCHHHHHHHHCCEEEECCCCEEEEECCCCCCCCCCEE
RVTHAQSADDYVSLGRDVESQVLARAIHAHIHGRVFLNGNKTIVFPPSPGSFASERMG
EEEECCCCHHHHHHCCCHHHHHHHHHHHHHHCEEEEECCCEEEEECCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]