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The map label for this gene is ligA [H]

Identifier: 150397271

GI number: 150397271

Start: 2122076

End: 2124229

Strand: Reverse

Name: ligA [H]

Synonym: Smed_2070

Alternate gene names: 150397271

Gene position: 2124229-2122076 (Counterclockwise)

Preceding gene: 150397272

Following gene: 150397269

Centisome position: 56.17

GC content: 63.56

Gene sequence:

>2154_bases
ATGCTGAATCAAAGAAAACCGGTCGAGCAAATGACCGAAGCCGAAGCCGCCGAGGAACTCGCCTTCCTGGCGGCGGAACT
TTCGCGCCACGACATGCTCTATCACGGGAAGGATGATCCGGAGATTTCGGACGCGGACTACGACGCGCTGAAGCGGCGCA
ACGATTTGATCGAGGAGCGATTTCCGGCGCTCATCCGCGAGGACAGCCCCTCGCAAAAGGTCGGTGCCGCACCTTCGCTG
ACCTTCGCGCCCGTAGTTCATGCGCGGCCGATGCTTTCGCTCGACAACAGTTTCTCGGACGAGGACGCCCGCGCATTCGT
CGCCGGCATCTACCGCTTTCTCGGGCGGCTCCCGGATGGATCGATCGCCTTTACGGTGGAACCGAAGATCGACGGTCTCT
CCATGTCGCTGCGTTACGAGAACCGTCGGCTGGTCACGGCCGCGACCCGCGGCGACGGCACGACCGGGGAAAATGTCACC
GCCAATGTCCGCACCATCGGCATGATACCGCAGAGGCTTCCTGCTGAAGCGCCCGACGTGGTCGAGATCCGCGGCGAAAT
CTATATGGCGAAATCCGACTTCGCCGCGCTCAATGCCGAAATGGCGGCGCAGGGCAGGCCACTCTACGTCAACCCCCGCA
ATACCGCATCCGGATCGCTCCGCCAGCTCGATGCCAAGGTGACCGCGAGCCGCAGGCTGCGCTTCTTCGCCTATGCCTGG
GGCGAGATGTCGGCGATGCCGGCGGATACGCAGCTCGGAATGGTGGAGACCTTCAAGGCCTGGGGATTCCCGGTCAATCC
GCTGATGCAGCGCTTCTTCTCCGCCGACGAGCTCCTGGAGCACTATCACCATATCGAGCGGGAGCGTCCGGAGCTGGACT
ACGACATCGACGGGGTCGTCTACAAGGTCGACCAGCTCGATCTGCAGGCTAGGCTCGGCTTCCGTTCGCGCAGCCCGCGC
TGGGCGACGGCGCACAAGTTCCCGGCAGAGCGGGCATTCACGCGGCTGAAGGGCATCGACATCCAGGTCGGGCGCACCGG
CGCGCTGACGCCTGTCGCGCGGCTGGAGCCAATCACGGTCGGGGGCGTGGTCGTGACCAATGCAACGCTGCACAACGAGG
ATTACATCCGCGGCATCGGCAATACGGGCGAACCGATCCGCGAAGGGCGCGACATCCGCATCGGCGACATGGTCATCGTT
CAGAGGGCAGGGGACGTGATTCCCCAGATCGTCGACGTGGTGATGGACGAGCGCCCTGAAGGGACCGAGCCTTACCGGTT
TCCGACGAGCTGCCCTATCTGCGGCAGTCACGCGGTTCGCGACATCAACGAGAAGACCGGCAAGGTAGATGCCGTGCGCC
GTTGCACGGGCGGTTTCGTCTGCCGCGCGCAGGCGGTCGAGCACCTGAAGCATTTCGTCTCGCGTAATGCCTTCGACATC
GAGGGGCTCGGCTCCAAGCAGATCGAGTTTTTCTTCGAAAGCGAGGACGAGACCCTCAGGATCCGCACCGCACCAGATAT
CTTCACGCTCGAACGCAGGCAGGAGTCCTCGCTTACGAAGCTCGAAAACATCGATGGCTTCGGCAAGGTCAGCGTCCGTA
AACTCTACGAGGCGATCAATGCCCGCCGCTCGATTGGGCTGCATCGCTTTATCTATGCGCTCGGAATCCGCCACGTCGGG
GAGACGACGGCCAAGCTTCTGGCGCGCTCCTACGGCACCTACGAGCACTTCGGCGCGGCCATGACCGAGGCGGGCGGCTT
TTCGGGAGACGCCTGGAACGAACTGAACAGCATCGACGGCATAGGCGAGGTGGTCGCCCGCGCCATCGTCGAATTCTACA
AGGAGCCGCGCAACCTGAAGGTCCTCTCCGACCTTCTGGAGGAGGTTACGCCTGAGCGTGCGGAGATGCCCGTTGCGACC
GACAGCCCCGTGGCCGGCAAGACGGTCGTCTTCACCGGTTCACTCGAAAAGATGACCCGCGACGAGGCCAAGGCGAAAGC
GGAGAGTCTCGGCGCCAAGGTCGCAGGCTCCGTCTCCAAGAAGACCGACATCGTCGTCGCAGGCCCTGGCGCCGGCTCGA
AGCTCGACAAGGCACGCGAACTCGGCCTCCAGACGATGGACGAGGACGAGTGGTTGGCGCTGATCGGCGGGTAG

Upstream 100 bases:

>100_bases
GCCTCGGAAGTGTGCATTGCTTGATGATTTTCTCCTCAAATCGATTCCGATTTAAGGAATCATGCAGTAAAAGCGACTCC
AAAGTTCTGGAGTCGCGTCC

Downstream 100 bases:

>100_bases
CGCAACTCACTATGTGCGGTCTTCCCCTCGATTCGATTTAGCTGGTGTCTGAATTATCTTTGTTCGGTCCGGTCGAGCCC
GAGTTTCGTGACCTCGTCGG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MLNQRKPVEQMTEAEAAEELAFLAAELSRHDMLYHGKDDPEISDADYDALKRRNDLIEERFPALIREDSPSQKVGAAPSL
TFAPVVHARPMLSLDNSFSDEDARAFVAGIYRFLGRLPDGSIAFTVEPKIDGLSMSLRYENRRLVTAATRGDGTTGENVT
ANVRTIGMIPQRLPAEAPDVVEIRGEIYMAKSDFAALNAEMAAQGRPLYVNPRNTASGSLRQLDAKVTASRRLRFFAYAW
GEMSAMPADTQLGMVETFKAWGFPVNPLMQRFFSADELLEHYHHIERERPELDYDIDGVVYKVDQLDLQARLGFRSRSPR
WATAHKFPAERAFTRLKGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIRGIGNTGEPIREGRDIRIGDMVIV
QRAGDVIPQIVDVVMDERPEGTEPYRFPTSCPICGSHAVRDINEKTGKVDAVRRCTGGFVCRAQAVEHLKHFVSRNAFDI
EGLGSKQIEFFFESEDETLRIRTAPDIFTLERRQESSLTKLENIDGFGKVSVRKLYEAINARRSIGLHRFIYALGIRHVG
ETTAKLLARSYGTYEHFGAAMTEAGGFSGDAWNELNSIDGIGEVVARAIVEFYKEPRNLKVLSDLLEEVTPERAEMPVAT
DSPVAGKTVVFTGSLEKMTRDEAKAKAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGLQTMDEDEWLALIGG

Sequences:

>Translated_717_residues
MLNQRKPVEQMTEAEAAEELAFLAAELSRHDMLYHGKDDPEISDADYDALKRRNDLIEERFPALIREDSPSQKVGAAPSL
TFAPVVHARPMLSLDNSFSDEDARAFVAGIYRFLGRLPDGSIAFTVEPKIDGLSMSLRYENRRLVTAATRGDGTTGENVT
ANVRTIGMIPQRLPAEAPDVVEIRGEIYMAKSDFAALNAEMAAQGRPLYVNPRNTASGSLRQLDAKVTASRRLRFFAYAW
GEMSAMPADTQLGMVETFKAWGFPVNPLMQRFFSADELLEHYHHIERERPELDYDIDGVVYKVDQLDLQARLGFRSRSPR
WATAHKFPAERAFTRLKGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIRGIGNTGEPIREGRDIRIGDMVIV
QRAGDVIPQIVDVVMDERPEGTEPYRFPTSCPICGSHAVRDINEKTGKVDAVRRCTGGFVCRAQAVEHLKHFVSRNAFDI
EGLGSKQIEFFFESEDETLRIRTAPDIFTLERRQESSLTKLENIDGFGKVSVRKLYEAINARRSIGLHRFIYALGIRHVG
ETTAKLLARSYGTYEHFGAAMTEAGGFSGDAWNELNSIDGIGEVVARAIVEFYKEPRNLKVLSDLLEEVTPERAEMPVAT
DSPVAGKTVVFTGSLEKMTRDEAKAKAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGLQTMDEDEWLALIGG
>Mature_717_residues
MLNQRKPVEQMTEAEAAEELAFLAAELSRHDMLYHGKDDPEISDADYDALKRRNDLIEERFPALIREDSPSQKVGAAPSL
TFAPVVHARPMLSLDNSFSDEDARAFVAGIYRFLGRLPDGSIAFTVEPKIDGLSMSLRYENRRLVTAATRGDGTTGENVT
ANVRTIGMIPQRLPAEAPDVVEIRGEIYMAKSDFAALNAEMAAQGRPLYVNPRNTASGSLRQLDAKVTASRRLRFFAYAW
GEMSAMPADTQLGMVETFKAWGFPVNPLMQRFFSADELLEHYHHIERERPELDYDIDGVVYKVDQLDLQARLGFRSRSPR
WATAHKFPAERAFTRLKGIDIQVGRTGALTPVARLEPITVGGVVVTNATLHNEDYIRGIGNTGEPIREGRDIRIGDMVIV
QRAGDVIPQIVDVVMDERPEGTEPYRFPTSCPICGSHAVRDINEKTGKVDAVRRCTGGFVCRAQAVEHLKHFVSRNAFDI
EGLGSKQIEFFFESEDETLRIRTAPDIFTLERRQESSLTKLENIDGFGKVSVRKLYEAINARRSIGLHRFIYALGIRHVG
ETTAKLLARSYGTYEHFGAAMTEAGGFSGDAWNELNSIDGIGEVVARAIVEFYKEPRNLKVLSDLLEEVTPERAEMPVAT
DSPVAGKTVVFTGSLEKMTRDEAKAKAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGLQTMDEDEWLALIGG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=706, Percent_Identity=47.3087818696884, Blast_Score=591, Evalue=1e-170,
Organism=Escherichia coli, GI87082305, Length=626, Percent_Identity=24.2811501597444, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 79297; Mature: 79297

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQRKPVEQMTEAEAAEELAFLAAELSRHDMLYHGKDDPEISDADYDALKRRNDLIEER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
FPALIREDSPSQKVGAAPSLTFAPVVHARPMLSLDNSFSDEDARAFVAGIYRFLGRLPDG
HHHHHCCCCCCCCCCCCCCCEECHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC
SIAFTVEPKIDGLSMSLRYENRRLVTAATRGDGTTGENVTANVRTIGMIPQRLPAEAPDV
CEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEEEEEEECCHHHCCCCCCCE
VEIRGEIYMAKSDFAALNAEMAAQGRPLYVNPRNTASGSLRQLDAKVTASRRLRFFAYAW
EEECCEEEEECCCHHHHCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCEEEEEEEC
GEMSAMPADTQLGMVETFKAWGFPVNPLMQRFFSADELLEHYHHIERERPELDYDIDGVV
CCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEE
YKVDQLDLQARLGFRSRSPRWATAHKFPAERAFTRLKGIDIQVGRTGALTPVARLEPITV
EEECHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHCCCEEE
GGVVVTNATLHNEDYIRGIGNTGEPIREGRDIRIGDMVIVQRAGDVIPQIVDVVMDERPE
CCEEEEEEEECCCHHHHCCCCCCCHHHCCCCEEECCEEEEEHHHHHHHHHHHHHHCCCCC
GTEPYRFPTSCPICGSHAVRDINEKTGKVDAVRRCTGGFVCRAQAVEHLKHFVSRNAFDI
CCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
EGLGSKQIEFFFESEDETLRIRTAPDIFTLERRQESSLTKLENIDGFGKVSVRKLYEAIN
CCCCCCEEEEEEECCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ARRSIGLHRFIYALGIRHVGETTAKLLARSYGTYEHFGAAMTEAGGFSGDAWNELNSIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCH
IGEVVARAIVEFYKEPRNLKVLSDLLEEVTPERAEMPVATDSPVAGKTVVFTGSLEKMTR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEEEECCHHHHHH
DEAKAKAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGLQTMDEDEWLALIGG
HHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MLNQRKPVEQMTEAEAAEELAFLAAELSRHDMLYHGKDDPEISDADYDALKRRNDLIEER
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
FPALIREDSPSQKVGAAPSLTFAPVVHARPMLSLDNSFSDEDARAFVAGIYRFLGRLPDG
HHHHHCCCCCCCCCCCCCCCEECHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHCCCCC
SIAFTVEPKIDGLSMSLRYENRRLVTAATRGDGTTGENVTANVRTIGMIPQRLPAEAPDV
CEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCEEEEEEEEECCHHHCCCCCCCE
VEIRGEIYMAKSDFAALNAEMAAQGRPLYVNPRNTASGSLRQLDAKVTASRRLRFFAYAW
EEECCEEEEECCCHHHHCHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHCEEEEEEEC
GEMSAMPADTQLGMVETFKAWGFPVNPLMQRFFSADELLEHYHHIERERPELDYDIDGVV
CCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEE
YKVDQLDLQARLGFRSRSPRWATAHKFPAERAFTRLKGIDIQVGRTGALTPVARLEPITV
EEECHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCCCHHHCCCEEE
GGVVVTNATLHNEDYIRGIGNTGEPIREGRDIRIGDMVIVQRAGDVIPQIVDVVMDERPE
CCEEEEEEEECCCHHHHCCCCCCCHHHCCCCEEECCEEEEEHHHHHHHHHHHHHHCCCCC
GTEPYRFPTSCPICGSHAVRDINEKTGKVDAVRRCTGGFVCRAQAVEHLKHFVSRNAFDI
CCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
EGLGSKQIEFFFESEDETLRIRTAPDIFTLERRQESSLTKLENIDGFGKVSVRKLYEAIN
CCCCCCEEEEEEECCCCEEEEEECCCEEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ARRSIGLHRFIYALGIRHVGETTAKLLARSYGTYEHFGAAMTEAGGFSGDAWNELNSIDG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCH
IGEVVARAIVEFYKEPRNLKVLSDLLEEVTPERAEMPVATDSPVAGKTVVFTGSLEKMTR
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCEEEEECCHHHHHH
DEAKAKAESLGAKVAGSVSKKTDIVVAGPGAGSKLDKARELGLQTMDEDEWLALIGG
HHHHHHHHHHCHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]