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The map label for this gene is panB [H]

Identifier: 150397260

GI number: 150397260

Start: 2110193

End: 2111014

Strand: Reverse

Name: panB [H]

Synonym: Smed_2059

Alternate gene names: 150397260

Gene position: 2111014-2110193 (Counterclockwise)

Preceding gene: 150397261

Following gene: 150397259

Centisome position: 55.82

GC content: 63.5

Gene sequence:

>822_bases
ATGAGCGTCCACACCACGAAACGGCGGCTCAGCCCCGCCAGCATCGAATCCCTGAAAGGCGACCGACCGATCGTAAGCCT
GACGGCCTATACGACGCCGATCGCGCGGCTGCTCGATCCCCATGTGGATTTCCTCCTCGTCGGCGATTCGCTCGGCATGG
TGCTTTACGGCCTCGACACGACCGTCGGCGTCACGCTCGACATGATGATTGCACATGGCCAGGCGGTCATGCGCGGATCG
GAGCGCTGTTGCGTCGTCGTCGATCTGCCTTTCGGCGCCTATCAGGAGTCGAAGGAGCAGGCGTTCCGCAGTGCCGCGCG
CATCCTGAAGGAAACGGGCTGCAGCGCAGTGAAGCTCGAGGGCGGGGCCGAGATGGCCGAGACGGTCGAATTCCTCGTCA
GCCGCGGCATTCCGGTGCTCGGCCATGTGGGCCTGATGCCGCAGCTCGTCAACACGACCGGCGGCTATCGCTCGGTCGGG
CGCAACGAGAAGGAAGTGGCCAAGATCCGCCGCGACGCCAAGGCGATCGACGATGCCGGTGCCTTCGCCATAGTGGTCGA
GGGGACGGTGGAGCCGGTTGCACGAGAGATCACCGCCACCCTGCGCGCGCCGACCATCGGTATCGGTGCTTCGCCGGCCT
GCGACGGCCAGATCCTTGTGTCGGACGATATGCTCGGCCTTTTCAATGATTTCAAACCGCGTTTCGTCAAGCATTTCGCC
GAGCTCGCCCCGGCGATCTCGAAGGCGGTCGAGGATTATGCCAATGAGGTGAAGGCGCGAACCTTTCCCGGCATAGAGCA
CACGTTTCAGGTAAAGCGTTGA

Upstream 100 bases:

>100_bases
CGGTCCTCATTTTGCTTTTCGTCCGCTTCGGCACGACGAAGCTGCTCGACAACCGCGTCATAGCCCCGAAATCCGCCCGT
TTTGCAAAGGTAGCGTGAGA

Downstream 100 bases:

>100_bases
CGTGTAGGGGCGGGCGCGATCCTTGCGCCCGCGTCCTTATGGTTGATCAAGCAACAAGTCTCGGGGGCCTTCCTTCCGGT
ATGACGCACGTCCAACCATC

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSVHTTKRRLSPASIESLKGDRPIVSLTAYTTPIARLLDPHVDFLLVGDSLGMVLYGLDTTVGVTLDMMIAHGQAVMRGS
ERCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLEGGAEMAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVG
RNEKEVAKIRRDAKAIDDAGAFAIVVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFKPRFVKHFA
ELAPAISKAVEDYANEVKARTFPGIEHTFQVKR

Sequences:

>Translated_273_residues
MSVHTTKRRLSPASIESLKGDRPIVSLTAYTTPIARLLDPHVDFLLVGDSLGMVLYGLDTTVGVTLDMMIAHGQAVMRGS
ERCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLEGGAEMAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVG
RNEKEVAKIRRDAKAIDDAGAFAIVVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFKPRFVKHFA
ELAPAISKAVEDYANEVKARTFPGIEHTFQVKR
>Mature_272_residues
SVHTTKRRLSPASIESLKGDRPIVSLTAYTTPIARLLDPHVDFLLVGDSLGMVLYGLDTTVGVTLDMMIAHGQAVMRGSE
RCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLEGGAEMAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVGR
NEKEVAKIRRDAKAIDDAGAFAIVVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFKPRFVKHFAE
LAPAISKAVEDYANEVKARTFPGIEHTFQVKR

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=266, Percent_Identity=40.2255639097744, Blast_Score=184, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6319653, Length=260, Percent_Identity=35, Blast_Score=155, Evalue=5e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 29386; Mature: 29255

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVHTTKRRLSPASIESLKGDRPIVSLTAYTTPIARLLDPHVDFLLVGDSLGMVLYGLDT
CCCCCCHHHCCCHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCEEEECCCCCEEEEECCC
TVGVTLDMMIAHGQAVMRGSERCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLE
HHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
GGAEMAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVGRNEKEVAKIRRDAKAIDDAG
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCC
AFAIVVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFKPRFVKHFA
CEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHH
ELAPAISKAVEDYANEVKARTFPGIEHTFQVKR
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC
>Mature Secondary Structure 
SVHTTKRRLSPASIESLKGDRPIVSLTAYTTPIARLLDPHVDFLLVGDSLGMVLYGLDT
CCCCCHHHCCCHHHHHCCCCCCEEEEEEHHHHHHHHHCCCCCEEEECCCCCEEEEECCC
TVGVTLDMMIAHGQAVMRGSERCCVVVDLPFGAYQESKEQAFRSAARILKETGCSAVKLE
HHHHHHHHHHHCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEC
GGAEMAETVEFLVSRGIPVLGHVGLMPQLVNTTGGYRSVGRNEKEVAKIRRDAKAIDDAG
CCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHCCCC
AFAIVVEGTVEPVAREITATLRAPTIGIGASPACDGQILVSDDMLGLFNDFKPRFVKHFA
CEEEEEECCHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCHHHHHHCCCHHHHHHHH
ELAPAISKAVEDYANEVKARTFPGIEHTFQVKR
HHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]