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The map label for this gene is nthA [H]

Identifier: 150397202

GI number: 150397202

Start: 2052703

End: 2053344

Strand: Reverse

Name: nthA [H]

Synonym: Smed_2001

Alternate gene names: 150397202

Gene position: 2053344-2052703 (Counterclockwise)

Preceding gene: 150397203

Following gene: 150397201

Centisome position: 54.29

GC content: 63.24

Gene sequence:

>642_bases
ATGTCCGAGCATCGTCATGGCCCTGGCGAAGAACACGGCCATCATCACGACAATCATCTCACCGACATGGAGGCGCGGGT
CAAGGCGCTGGAGACGGTGTTGACGGAAAAAGGGCTGATCGATCCTGCGGCGATCGACGCGATCGTCGACACATACGAGA
CGAAGGTGGGACCGCGAAACGGTGCACGCGTCGTCGCCAAGGCCTGGAGCGATCCGGATTTTGCCGATTGGCTGAGACGT
GACGCCACCGCGGCGATCGCCAGCCTCGGCTTCACCGGACGCCAGGGCGAGCACATGCGTGCGGTGTTCAATACGTCCGA
AACGCACAACCTCATCGTCTGTACGCTCTGCTCCTGCTATCCCTGGGCAGTGCTGGGGCTGCCGCCGGTCTGGTACAAGG
CGCCGCCCTATCGATCGCGGGCCGTTATCGATCCGCGGGGCGTTCTTGCAGAATTCGGGCTTAACCTGCCGGCTGAAAAG
AAGATCCGCGTCTGGGATTCGACCGCAGAGCTGCGCTATCTGGTGGTGCCGGAGCGGCCGGCGGCCACCGACGATCTCGG
CGAGGATGCCCTGGCAAAGCTCGTCACGCGCGATTCGATGATCGGCACCGGGCTCGCCCTTTCACCGGAGGCTTTTCGAT
GA

Upstream 100 bases:

>100_bases
TGGCCGATGCTCAGGTAAGCCAATGGGCATCTTCAGAAAATGATTCTGCTTCATCGCCCAATGCATTAATAATAGACGCA
TTTTCGGAAAGGATTACGGA

Downstream 100 bases:

>100_bases
ACGGTCCGCATGATCTCGGCGGTCAGCATGGCATGGGCCCGATCGCTCCGGAATGGAACGAGCCCACGTTCCACGCGGAA
TGGGAGAAGCGTGCGCTTGG

Product: nitrile hydratase subunit alpha

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSEHRHGPGEEHGHHHDNHLTDMEARVKALETVLTEKGLIDPAAIDAIVDTYETKVGPRNGARVVAKAWSDPDFADWLRR
DATAAIASLGFTGRQGEHMRAVFNTSETHNLIVCTLCSCYPWAVLGLPPVWYKAPPYRSRAVIDPRGVLAEFGLNLPAEK
KIRVWDSTAELRYLVVPERPAATDDLGEDALAKLVTRDSMIGTGLALSPEAFR

Sequences:

>Translated_213_residues
MSEHRHGPGEEHGHHHDNHLTDMEARVKALETVLTEKGLIDPAAIDAIVDTYETKVGPRNGARVVAKAWSDPDFADWLRR
DATAAIASLGFTGRQGEHMRAVFNTSETHNLIVCTLCSCYPWAVLGLPPVWYKAPPYRSRAVIDPRGVLAEFGLNLPAEK
KIRVWDSTAELRYLVVPERPAATDDLGEDALAKLVTRDSMIGTGLALSPEAFR
>Mature_212_residues
SEHRHGPGEEHGHHHDNHLTDMEARVKALETVLTEKGLIDPAAIDAIVDTYETKVGPRNGARVVAKAWSDPDFADWLRRD
ATAAIASLGFTGRQGEHMRAVFNTSETHNLIVCTLCSCYPWAVLGLPPVWYKAPPYRSRAVIDPRGVLAEFGLNLPAEKK
IRVWDSTAELRYLVVPERPAATDDLGEDALAKLVTRDSMIGTGLALSPEAFR

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides. Industrial production of acrylamide is now being developed using some of the enzymes of this class [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit alpha family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004232
- InterPro:   IPR018141
- ProDom:   PD007559 [H]

Pfam domain/function: PF02979 NHase_alpha [H]

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 23473; Mature: 23342

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEHRHGPGEEHGHHHDNHLTDMEARVKALETVLTEKGLIDPAAIDAIVDTYETKVGPRN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
GARVVAKAWSDPDFADWLRRDATAAIASLGFTGRQGEHMRAVFNTSETHNLIVCTLCSCY
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
PWAVLGLPPVWYKAPPYRSRAVIDPRGVLAEFGLNLPAEKKIRVWDSTAELRYLVVPERP
CHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCC
AATDDLGEDALAKLVTRDSMIGTGLALSPEAFR
CCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCC
>Mature Secondary Structure 
SEHRHGPGEEHGHHHDNHLTDMEARVKALETVLTEKGLIDPAAIDAIVDTYETKVGPRN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCC
GARVVAKAWSDPDFADWLRRDATAAIASLGFTGRQGEHMRAVFNTSETHNLIVCTLCSCY
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEEECCCC
PWAVLGLPPVWYKAPPYRSRAVIDPRGVLAEFGLNLPAEKKIRVWDSTAELRYLVVPERP
CHHHHCCCCCCCCCCCCCCCEEECCHHHHHHHCCCCCCCCEEEEECCCCCEEEEEECCCC
AATDDLGEDALAKLVTRDSMIGTGLALSPEAFR
CCCCCCCHHHHHHHHHHHHHHCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]