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The map label for this gene is nthB [H]

Identifier: 150397201

GI number: 150397201

Start: 2052047

End: 2052706

Strand: Reverse

Name: nthB [H]

Synonym: Smed_2000

Alternate gene names: 150397201

Gene position: 2052706-2052047 (Counterclockwise)

Preceding gene: 150397202

Following gene: 150397196

Centisome position: 54.28

GC content: 65.0

Gene sequence:

>660_bases
ATGAACGGTCCGCATGATCTCGGCGGTCAGCATGGCATGGGCCCGATCGCTCCGGAATGGAACGAGCCCACGTTCCACGC
GGAATGGGAGAAGCGTGCGCTTGGCATTACGCTTTCCTGTGGCGCTTTCGGCGCCTGGACGCTGGACGAAAGCCGGCACG
CGCGTGAAAGCCTGCCCCCGGCGACCTATCTCTCGGCGAGCTATTATGAAATCTGGACACGAGCCCTGGAAACGCTGCTC
AAGCGCCACGGCTTCCTGACAGAAGCCGAGCTCGACGCCGGCCGTATGCTCGACAAGTGGCGGGAGCCCAAACGGGTGCT
CGCAGCGGACATGGTTGCCGGCGTGCTCGCCAAGGGAGCTGCGTGCGACCGGCCGGTGGAAGCGCCGCCGCGCTTTGCCG
CCGGCGAGAGCGTGCGTACGAAGAACTTCAATCCGGAGAGTCACACACGCCTCCCGCGTTACGCCCGTGCCCGGAGGGGC
GTGGTGGAGGCCGTACAGGGCAGCTTCGTCTTTCCGGACGACAACGCTCACGGCAGGGGCGAGAATCCGCAATGGCTCTA
TACCGTCGTCTTCGGCGCCCGGGAGATCTGGGGCGAGGGCGCGGACCCGACGCATACCATCTCCATCGATGCATGGGAGA
GCTACCTTGAACCTCTATGA

Upstream 100 bases:

>100_bases
CGGCGGCCACCGACGATCTCGGCGAGGATGCCCTGGCAAAGCTCGTCACGCGCGATTCGATGATCGGCACCGGGCTCGCC
CTTTCACCGGAGGCTTTTCG

Downstream 100 bases:

>100_bases
CGCGAGTCTTGCCCGGTCGCCGCTCCTTGCTTCGGCAGAACTGCCGAAATCGCGGGAGGGCGACCCCGTCTTTGCCGAAC
CCTGGCAGGCGGCCGTCTTC

Product: nitrile hydratase

Products: NA

Alternate protein names: NHase; Nitrilase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNGPHDLGGQHGMGPIAPEWNEPTFHAEWEKRALGITLSCGAFGAWTLDESRHARESLPPATYLSASYYEIWTRALETLL
KRHGFLTEAELDAGRMLDKWREPKRVLAADMVAGVLAKGAACDRPVEAPPRFAAGESVRTKNFNPESHTRLPRYARARRG
VVEAVQGSFVFPDDNAHGRGENPQWLYTVVFGAREIWGEGADPTHTISIDAWESYLEPL

Sequences:

>Translated_219_residues
MNGPHDLGGQHGMGPIAPEWNEPTFHAEWEKRALGITLSCGAFGAWTLDESRHARESLPPATYLSASYYEIWTRALETLL
KRHGFLTEAELDAGRMLDKWREPKRVLAADMVAGVLAKGAACDRPVEAPPRFAAGESVRTKNFNPESHTRLPRYARARRG
VVEAVQGSFVFPDDNAHGRGENPQWLYTVVFGAREIWGEGADPTHTISIDAWESYLEPL
>Mature_219_residues
MNGPHDLGGQHGMGPIAPEWNEPTFHAEWEKRALGITLSCGAFGAWTLDESRHARESLPPATYLSASYYEIWTRALETLL
KRHGFLTEAELDAGRMLDKWREPKRVLAADMVAGVLAKGAACDRPVEAPPRFAAGESVRTKNFNPESHTRLPRYARARRG
VVEAVQGSFVFPDDNAHGRGENPQWLYTVVFGAREIWGEGADPTHTISIDAWESYLEPL

Specific function: NHase catalyzes the hydration of various nitrile compounds to the corresponding amides [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrile hydratase subunit beta family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008990
- InterPro:   IPR003168 [H]

Pfam domain/function: NA

EC number: =4.2.1.84 [H]

Molecular weight: Translated: 24380; Mature: 24380

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPHDLGGQHGMGPIAPEWNEPTFHAEWEKRALGITLSCGAFGAWTLDESRHARESLPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEEECCCHHHHHHHCCC
ATYLSASYYEIWTRALETLLKRHGFLTEAELDAGRMLDKWREPKRVLAADMVAGVLAKGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCC
ACDRPVEAPPRFAAGESVRTKNFNPESHTRLPRYARARRGVVEAVQGSFVFPDDNAHGRG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCCC
ENPQWLYTVVFGAREIWGEGADPTHTISIDAWESYLEPL
CCCCEEEEEEHHHHHHHCCCCCCCEEEEHHHHHHHHCCC
>Mature Secondary Structure
MNGPHDLGGQHGMGPIAPEWNEPTFHAEWEKRALGITLSCGAFGAWTLDESRHARESLPP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCEEEEEEECCCCEEEECCCHHHHHHHCCC
ATYLSASYYEIWTRALETLLKRHGFLTEAELDAGRMLDKWREPKRVLAADMVAGVLAKGA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCCC
ACDRPVEAPPRFAAGESVRTKNFNPESHTRLPRYARARRGVVEAVQGSFVFPDDNAHGRG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCEECCCCCCCCCC
ENPQWLYTVVFGAREIWGEGADPTHTISIDAWESYLEPL
CCCCEEEEEEHHHHHHHCCCCCCCEEEEHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9154927 [H]