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The map label for this gene is potD [C]

Identifier: 150397150

GI number: 150397150

Start: 2002028

End: 2003008

Strand: Reverse

Name: potD [C]

Synonym: Smed_1948

Alternate gene names: 150397150

Gene position: 2003008-2002028 (Counterclockwise)

Preceding gene: 150397154

Following gene: 150397149

Centisome position: 52.96

GC content: 62.9

Gene sequence:

>981_bases
ATGAAAACGCTTCTCCCTGCCGTCGCCGCAGCGCTCGTCGCCGGCCTCCTGTCCACTGCCGCTCTCGCCGAAAGCCTCGT
GCTTTATACCAGTCAGCCGAACGAGGACGCGCAGGCGACGGTGGATGCATTCGAGGCGGCCAACCCCGGCGTCGAGGTGG
AATGGGTGCGGGAGGGAACAACAAAAATCATGGCTAAGCTGATGGCCGAGATCGAGGCGGGCAACCCCGTGGCGGATGTG
CTTCTGATCGCCGATACGGTGACGATGCAGCGGTTGAAGGAGGCGGGCCAGCTGATGCCTTACAAATCTCCGGAAGCCTC
AGCTTTCGAGGCCTCTCTCTTCGACCCGGACGGCACCTATTATTCGACGAAGATGATCACCACCGGGATCATCTACAACA
CTTCCGCCGCGATGAAGCCGGCCGGCTGGGAGGATCTTGCGAAACCCGAGGCCAAGGGGCTCGTCACCATGCCCAGCCCG
CTCACGTCAGGCGCGGCGCTGATCCATGCCCAGACGCTTGCCGGCATCGGTGCGCTCGGTTGGGACTACTACGAGGCGCT
CGCGGAAAACGGCGCGACGGCCGCCGGCGGCAATGGCGGCGTGTTGAAGTCCGTCGCAACGGGCGAGAAGGCCTATGGGA
TGGTGGTGGATTTCATGGCGATCCGCGAGAAGGCAAAGGGCGCGCCGGTGGAGTTCGTCTTTCCGGCGGAGGGCGTTTCG
GCCGTTACCGAGCCGGTCGCCATCCTGAGAACCGCAAAAAACCCGGATGCAGCAAAGAAATTCGTCGATTTCCTCCTTTC
GGAAGAGGGGCAGCAGGTGGCAGTGACGATGGGCTACATTCCGGCCCGCAACGGGCTTGCCTTGCCCGAGGGATTTCCCG
CCCGCGAGGTTGTTAAGGTGCTGCCGGTCGACGCCGCCGCAGCCGTGAAGAATTCCGACGCGGATCTGAAAACCTTCTCG
GGGATTTTCGGCACCAACTGA

Upstream 100 bases:

>100_bases
TCGCCGGCGGACGGGTTGAAGGGGGCGGTCCCGTTGTCACAAGGCTGTAAAAGACGCGCTCTATCCCTCGGTGCGATCTT
CTCCGACTGAGGGCAAACTC

Downstream 100 bases:

>100_bases
CCCTCCGATGCGCGTTTCCGACGAGATCGCCAGAGGTCGGCGCAAGGCAACGCATGCGCCGGCCGTGGACACCGATGCTG
AAGTACGCTCCGGCTCGAGA

Product: extracellular solute-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKTLLPAVAAALVAGLLSTAALAESLVLYTSQPNEDAQATVDAFEAANPGVEVEWVREGTTKIMAKLMAEIEAGNPVADV
LLIADTVTMQRLKEAGQLMPYKSPEASAFEASLFDPDGTYYSTKMITTGIIYNTSAAMKPAGWEDLAKPEAKGLVTMPSP
LTSGAALIHAQTLAGIGALGWDYYEALAENGATAAGGNGGVLKSVATGEKAYGMVVDFMAIREKAKGAPVEFVFPAEGVS
AVTEPVAILRTAKNPDAAKKFVDFLLSEEGQQVAVTMGYIPARNGLALPEGFPAREVVKVLPVDAAAAVKNSDADLKTFS
GIFGTN

Sequences:

>Translated_326_residues
MKTLLPAVAAALVAGLLSTAALAESLVLYTSQPNEDAQATVDAFEAANPGVEVEWVREGTTKIMAKLMAEIEAGNPVADV
LLIADTVTMQRLKEAGQLMPYKSPEASAFEASLFDPDGTYYSTKMITTGIIYNTSAAMKPAGWEDLAKPEAKGLVTMPSP
LTSGAALIHAQTLAGIGALGWDYYEALAENGATAAGGNGGVLKSVATGEKAYGMVVDFMAIREKAKGAPVEFVFPAEGVS
AVTEPVAILRTAKNPDAAKKFVDFLLSEEGQQVAVTMGYIPARNGLALPEGFPAREVVKVLPVDAAAAVKNSDADLKTFS
GIFGTN
>Mature_326_residues
MKTLLPAVAAALVAGLLSTAALAESLVLYTSQPNEDAQATVDAFEAANPGVEVEWVREGTTKIMAKLMAEIEAGNPVADV
LLIADTVTMQRLKEAGQLMPYKSPEASAFEASLFDPDGTYYSTKMITTGIIYNTSAAMKPAGWEDLAKPEAKGLVTMPSP
LTSGAALIHAQTLAGIGALGWDYYEALAENGATAAGGNGGVLKSVATGEKAYGMVVDFMAIREKAKGAPVEFVFPAEGVS
AVTEPVAILRTAKNPDAAKKFVDFLLSEEGQQVAVTMGYIPARNGLALPEGFPAREVVKVLPVDAAAAVKNSDADLKTFS
GIFGTN

Specific function: Required For The Activity Of The Bacterial Periplasmic Transport System Of Putrescine And Spermidine. Polyamine Binding Protein. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 33906; Mature: 33906

Theoretical pI: Translated: 4.37; Mature: 4.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLPAVAAALVAGLLSTAALAESLVLYTSQPNEDAQATVDAFEAANPGVEVEWVREGT
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCH
TKIMAKLMAEIEAGNPVADVLLIADTVTMQRLKEAGQLMPYKSPEASAFEASLFDPDGTY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCE
YSTKMITTGIIYNTSAAMKPAGWEDLAKPEAKGLVTMPSPLTSGAALIHAQTLAGIGALG
EEEEEEEEEEEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
WDYYEALAENGATAAGGNGGVLKSVATGEKAYGMVVDFMAIREKAKGAPVEFVFPAEGVS
HHHHHHHHHCCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AVTEPVAILRTAKNPDAAKKFVDFLLSEEGQQVAVTMGYIPARNGLALPEGFPAREVVKV
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHH
LPVDAAAAVKNSDADLKTFSGIFGTN
HCCCHHHHHCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKTLLPAVAAALVAGLLSTAALAESLVLYTSQPNEDAQATVDAFEAANPGVEVEWVREGT
CCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCH
TKIMAKLMAEIEAGNPVADVLLIADTVTMQRLKEAGQLMPYKSPEASAFEASLFDPDGTY
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCE
YSTKMITTGIIYNTSAAMKPAGWEDLAKPEAKGLVTMPSPLTSGAALIHAQTLAGIGALG
EEEEEEEEEEEEECCCCCCCCCCHHHCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHH
WDYYEALAENGATAAGGNGGVLKSVATGEKAYGMVVDFMAIREKAKGAPVEFVFPAEGVS
HHHHHHHHHCCCCCCCCCCCEEHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCHH
AVTEPVAILRTAKNPDAAKKFVDFLLSEEGQQVAVTMGYIPARNGLALPEGFPAREVVKV
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHHH
LPVDAAAAVKNSDADLKTFSGIFGTN
HCCCHHHHHCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]