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The map label for this gene is yitJ [H]

Identifier: 150396986

GI number: 150396986

Start: 1865800

End: 1866903

Strand: Direct

Name: yitJ [H]

Synonym: Smed_1783

Alternate gene names: 150396986

Gene position: 1865800-1866903 (Clockwise)

Preceding gene: 150396985

Following gene: 150396987

Centisome position: 49.33

GC content: 62.41

Gene sequence:

>1104_bases
ATGAGCCCGGATATCAATCCTTACGATCCGGGCGCCCCGCTCGACCCGCTCCCCGGCCATTCTTCGCGCGGGCGGCTGGA
GCGCGTTCTGCGTCGCGGTGAATTCGCCGTGACCGCCGAGCTCAACCCGCCGGACAGCGCCAATCCGCATGACGTCTACG
AGCGCGCGGCGATCTTCGACGGGTGGGTTGACGGCATCAACGCAGTCGACGCCTCGGGCGCCAATTGCCATATGTCTTCC
GTCGGTATCTGCGCGCTGCTGACGCGCATGGGCTATGCGCCGATCATGCAGATCGCCTGCCGGGACAGAAATCGCATCGC
CATTCAGGGCGACGTTCTCGGCGCCTCGGCCATGGGCGTCCAGAACATCATGTGCTTGACGGGCGACGGCGTACAGGCCG
GCGACCAGCCCGGGGCCAAGCCCGTCTTCGATCTGGACTGCATGTCCCTGCTCGAGACGGTGCGCATCATGCGCGACAAT
TCCAAGTTTCTTTCGGGCCGCAAGCTATCGACGCCGCCCCACGTATTTCTTGGAGCGGCAATCAACCCTTTCGCCCCTCC
CTACGATTTCCGCCCCTACCGCCTCGCCAAGAAGATCGAAGCCGGCGCTCAATTCGTCCAGAGCCAGTATTGCTTCGACG
TTCCGATGTTCCGCGAATATATGAAAAAGGTGCGCGACCTCGGTTTGCACGAGAAGTGCTTCATCCTGGTGGGCGTCGGG
CCGTTGGCTTCCGCCAAGACTGCCCGCTGGATCCGATCCAATGTTCCTGGCATCCACATTCCGGATGGCATTATCAGGCG
GCTCGAGGGCGCCCAGGATCAGAAGAAGGAAGGCAAGCAGCTCTGCATCGACGTCATGAACGAGGTGAAGGAGATCGAGG
GTGTCTCAGGCGTCCATGTCATGGCGTACCGCCAGGAGGAGTATGTCGCGGAAATCGTACATGAATCAGGCGTCCTGAGG
GGCCGCAAGCCGTGGAAGCGCGAAGCCGCGCCGACCGATGCGATGGTTGCCGAACGGCTGGAGCATATTCGCGAAGGCAG
GGAGGAAAACCAGCAGCAGATGGCCGAGGCCGCCGCGCACCACCCGCATGAGACAAAGCAGTGA

Upstream 100 bases:

>100_bases
AGCCGGTCAATCAGTCGCTGCGCGAGACCTCTTCCTGGTTGCGGGTGACGGCCGAAGCCGCGGCCGCCCGCGAAGCAGCG
AAATCGGAGGGCCAGCAGCT

Downstream 100 bases:

>100_bases
ACCAGCGGCTTTACTGCATGTTTCCTTTCATCCAATGCGGTAAGGAAGCATGCAGCAGGTCAAAGTGCTCGGACCGTCCG
GCACGCTACAAAAACACGAA

Product: methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSPDINPYDPGAPLDPLPGHSSRGRLERVLRRGEFAVTAELNPPDSANPHDVYERAAIFDGWVDGINAVDASGANCHMSS
VGICALLTRMGYAPIMQIACRDRNRIAIQGDVLGASAMGVQNIMCLTGDGVQAGDQPGAKPVFDLDCMSLLETVRIMRDN
SKFLSGRKLSTPPHVFLGAAINPFAPPYDFRPYRLAKKIEAGAQFVQSQYCFDVPMFREYMKKVRDLGLHEKCFILVGVG
PLASAKTARWIRSNVPGIHIPDGIIRRLEGAQDQKKEGKQLCIDVMNEVKEIEGVSGVHVMAYRQEEYVAEIVHESGVLR
GRKPWKREAAPTDAMVAERLEHIREGREENQQQMAEAAAHHPHETKQ

Sequences:

>Translated_367_residues
MSPDINPYDPGAPLDPLPGHSSRGRLERVLRRGEFAVTAELNPPDSANPHDVYERAAIFDGWVDGINAVDASGANCHMSS
VGICALLTRMGYAPIMQIACRDRNRIAIQGDVLGASAMGVQNIMCLTGDGVQAGDQPGAKPVFDLDCMSLLETVRIMRDN
SKFLSGRKLSTPPHVFLGAAINPFAPPYDFRPYRLAKKIEAGAQFVQSQYCFDVPMFREYMKKVRDLGLHEKCFILVGVG
PLASAKTARWIRSNVPGIHIPDGIIRRLEGAQDQKKEGKQLCIDVMNEVKEIEGVSGVHVMAYRQEEYVAEIVHESGVLR
GRKPWKREAAPTDAMVAERLEHIREGREENQQQMAEAAAHHPHETKQ
>Mature_366_residues
SPDINPYDPGAPLDPLPGHSSRGRLERVLRRGEFAVTAELNPPDSANPHDVYERAAIFDGWVDGINAVDASGANCHMSSV
GICALLTRMGYAPIMQIACRDRNRIAIQGDVLGASAMGVQNIMCLTGDGVQAGDQPGAKPVFDLDCMSLLETVRIMRDNS
KFLSGRKLSTPPHVFLGAAINPFAPPYDFRPYRLAKKIEAGAQFVQSQYCFDVPMFREYMKKVRDLGLHEKCFILVGVGP
LASAKTARWIRSNVPGIHIPDGIIRRLEGAQDQKKEGKQLCIDVMNEVKEIEGVSGVHVMAYRQEEYVAEIVHESGVLRG
RKPWKREAAPTDAMVAERLEHIREGREENQQQMAEAAAHHPHETKQ

Specific function: Unknown

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 40573; Mature: 40442

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDINPYDPGAPLDPLPGHSSRGRLERVLRRGEFAVTAELNPPDSANPHDVYERAAIFD
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GWVDGINAVDASGANCHMSSVGICALLTRMGYAPIMQIACRDRNRIAIQGDVLGASAMGV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHCC
QNIMCLTGDGVQAGDQPGAKPVFDLDCMSLLETVRIMRDNSKFLSGRKLSTPPHVFLGAA
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEECCC
INPFAPPYDFRPYRLAKKIEAGAQFVQSQYCFDVPMFREYMKKVRDLGLHEKCFILVGVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
PLASAKTARWIRSNVPGIHIPDGIIRRLEGAQDQKKEGKQLCIDVMNEVKEIEGVSGVHV
CCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MAYRQEEYVAEIVHESGVLRGRKPWKREAAPTDAMVAERLEHIREGREENQQQMAEAAAH
EEECHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HPHETKQ
CCCCCCC
>Mature Secondary Structure 
SPDINPYDPGAPLDPLPGHSSRGRLERVLRRGEFAVTAELNPPDSANPHDVYERAAIFD
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHH
GWVDGINAVDASGANCHMSSVGICALLTRMGYAPIMQIACRDRNRIAIQGDVLGASAMGV
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEECCCCCCHHHCC
QNIMCLTGDGVQAGDQPGAKPVFDLDCMSLLETVRIMRDNSKFLSGRKLSTPPHVFLGAA
CEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEECCC
INPFAPPYDFRPYRLAKKIEAGAQFVQSQYCFDVPMFREYMKKVRDLGLHEKCFILVGVG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECC
PLASAKTARWIRSNVPGIHIPDGIIRRLEGAQDQKKEGKQLCIDVMNEVKEIEGVSGVHV
CCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEE
MAYRQEEYVAEIVHESGVLRGRKPWKREAAPTDAMVAERLEHIREGREENQQQMAEAAAH
EEECHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
HPHETKQ
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]