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The map label for this gene is metH [H]

Identifier: 150396987

GI number: 150396987

Start: 1867049

End: 1868008

Strand: Direct

Name: metH [H]

Synonym: Smed_1784

Alternate gene names: 150396987

Gene position: 1867049-1868008 (Clockwise)

Preceding gene: 150396986

Following gene: 150396988

Centisome position: 49.37

GC content: 64.69

Gene sequence:

>960_bases
ATGACTCGCACCATCGTCGCCTCCGCCACCCGTGAGATCGTGATCGGCTTCGACCAGCCCTTCTGCGTCATCGGCGAACG
CATCAATCCGACCGGCCGAAAGAAGCTCGCCGCCGAAATGCTCGAAGGCAACTTCGACACAGTGATCAAGGATGCGCTGG
AGCAGGTCGCCGCCGGAGCTACGATGCTCGACGTCAATGCGGGCGTGACAGCCGTCAACCCGAACGAGACCGAGCCTCCG
CTCCTCGTCAAAACACTCGAGATCGTCCAAGGCCTCATCGACGTACCGCTGTCGATCGACAGCTCGGTGACCGCAGCCAT
CGAGGCGGGCTTGCGTGTCGCCAAGGGCCGCCCGCTGGTGAACTCGGTCACCGGCGAAGAAGAGAAGCTGGAAGCGATCC
TGCCGCTCATCAAGAAATACGACGTCCCCGTCGTAGCCATCTCCAACGACGAAACCGGCATTTCCATGGATCCGGACGTT
CGCTTTGCGGTCGCCAAGAAGATCGTTGAGCGCGCCATGGACCACGGGATCAAGCCCCACGATATCGTCGTCGATCCGCT
CGTCATGCCCATCGGGGCCCTGGGCGATGCCGGCCGGCAGGTCTTCGCGCTTCTGCGCCGCCTGCGCGAGGAGCTGAAGG
TCAACACGACCTGCGGCCTCTCCAACATCTCCTTCGGCCTGCCGCACCGCCACGGCATCAATGCCGGCTTCATTCCGATG
GTTATCGGTGCCGGCATGACCTCGGCAATCATGAACCCCTGCCGCCCGCAGGAGATGGAGGCGGTTCGCGCGGCCAACGT
GCTTAACGGCACCGACCCGAACTGCACGAACTGGATCATGACCTATCGCGACCACAAGCCGGCCGAAGGAGGCCACGCCG
TGGCGGCCGCTGCCCCGTCGGCATCCGGAGGACGGCGCGGAGGCCGCGCAGCCCGCGCCGGCATCGCTCGGGCGGAATAA

Upstream 100 bases:

>100_bases
AAGCATGCAGCAGGTCAAAGTGCTCGGACCGTCCGGCACGCTACAAAAACACGAACGGCCATTTCATGAAGGCCATCGCT
CGAGGATCAGAGGATCTTAT

Downstream 100 bases:

>100_bases
AATGGCAAAGCATCACTCCTTCACAAGCGATCTGATTGCACTCTGGTTTCAGGCGCCGATGGTCATCGCCGCGCGCACGC
AGGCGATGGCGGTGGCCGCG

Product: methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTRTIVASATREIVIGFDQPFCVIGERINPTGRKKLAAEMLEGNFDTVIKDALEQVAAGATMLDVNAGVTAVNPNETEPP
LLVKTLEIVQGLIDVPLSIDSSVTAAIEAGLRVAKGRPLVNSVTGEEEKLEAILPLIKKYDVPVVAISNDETGISMDPDV
RFAVAKKIVERAMDHGIKPHDIVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAGFIPM
VIGAGMTSAIMNPCRPQEMEAVRAANVLNGTDPNCTNWIMTYRDHKPAEGGHAVAAAAPSASGGRRGGRAARAGIARAE

Sequences:

>Translated_319_residues
MTRTIVASATREIVIGFDQPFCVIGERINPTGRKKLAAEMLEGNFDTVIKDALEQVAAGATMLDVNAGVTAVNPNETEPP
LLVKTLEIVQGLIDVPLSIDSSVTAAIEAGLRVAKGRPLVNSVTGEEEKLEAILPLIKKYDVPVVAISNDETGISMDPDV
RFAVAKKIVERAMDHGIKPHDIVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAGFIPM
VIGAGMTSAIMNPCRPQEMEAVRAANVLNGTDPNCTNWIMTYRDHKPAEGGHAVAAAAPSASGGRRGGRAARAGIARAE
>Mature_318_residues
TRTIVASATREIVIGFDQPFCVIGERINPTGRKKLAAEMLEGNFDTVIKDALEQVAAGATMLDVNAGVTAVNPNETEPPL
LVKTLEIVQGLIDVPLSIDSSVTAAIEAGLRVAKGRPLVNSVTGEEEKLEAILPLIKKYDVPVVAISNDETGISMDPDVR
FAVAKKIVERAMDHGIKPHDIVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAGFIPMV
IGAGMTSAIMNPCRPQEMEAVRAANVLNGTDPNCTNWIMTYRDHKPAEGGHAVAAAAPSASGGRRGGRAARAGIARAE

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=249, Percent_Identity=31.3253012048193, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790450, Length=258, Percent_Identity=29.0697674418605, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535405, Length=254, Percent_Identity=30.3149606299213, Blast_Score=95, Evalue=5e-20,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 33802; Mature: 33670

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTIVASATREIVIGFDQPFCVIGERINPTGRKKLAAEMLEGNFDTVIKDALEQVAAGA
CCCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TMLDVNAGVTAVNPNETEPPLLVKTLEIVQGLIDVPLSIDSSVTAAIEAGLRVAKGRPLV
EEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHCCCCCH
NSVTGEEEKLEAILPLIKKYDVPVVAISNDETGISMDPDVRFAVAKKIVERAMDHGIKPH
HCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DIVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAGFIPM
CEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VIGAGMTSAIMNPCRPQEMEAVRAANVLNGTDPNCTNWIMTYRDHKPAEGGHAVAAAAPS
HHHCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
ASGGRRGGRAARAGIARAE
CCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure 
TRTIVASATREIVIGFDQPFCVIGERINPTGRKKLAAEMLEGNFDTVIKDALEQVAAGA
CCEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TMLDVNAGVTAVNPNETEPPLLVKTLEIVQGLIDVPLSIDSSVTAAIEAGLRVAKGRPLV
EEEEECCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHCCCCCH
NSVTGEEEKLEAILPLIKKYDVPVVAISNDETGISMDPDVRFAVAKKIVERAMDHGIKPH
HCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DIVVDPLVMPIGALGDAGRQVFALLRRLREELKVNTTCGLSNISFGLPHRHGINAGFIPM
CEEECHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHH
VIGAGMTSAIMNPCRPQEMEAVRAANVLNGTDPNCTNWIMTYRDHKPAEGGHAVAAAAPS
HHHCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCC
ASGGRRGGRAARAGIARAE
CCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]