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The map label for this gene is yitJ [H]

Identifier: 150396974

GI number: 150396974

Start: 1854761

End: 1855774

Strand: Direct

Name: yitJ [H]

Synonym: Smed_1771

Alternate gene names: 150396974

Gene position: 1854761-1855774 (Clockwise)

Preceding gene: 150396973

Following gene: 150396979

Centisome position: 49.04

GC content: 62.92

Gene sequence:

>1014_bases
ATGTCCGTTGCACCGCATGCTCTTGCCGACCTCCTCGCCCAGAAGGGTGTCCTGCTTGCCGATGGTGCTACCGGCACATC
GCTCTTCGCCATGGGGCTCGAAGCGGGCGAAGCACCGGAGATCTGGAACGAGACGAAACCGGAAAACATAACCAAGCTGC
ATCAGGACTTCGTAGATGCGGGCGCGGACATCATTCTTACCAATTCCTTTGGTGGCACGCGCCACCGGTTGAAGCTTCAT
CAGGCCGAAGACCGGGTCCATCAGCTCAACAAGCGCGCTGCGGAAATCGCGCGCGCAGTCGCTGACAAAGCGCCGCGAAA
GGTCATTACGGCTGGCTCGGTCGGCCCGACCGGCGAACTGCTGATTCCGCTCGGCGCACTCTCCTATGAGGACGCCGTCG
CAGCCTTTGTGGAACAGATCGAGGGGCTGAAGGCCGGCGGTGCGGAAGTTGCCTGGATCGAAACCATGTCTTCTGCGGAC
GAAATCCGCGCAGCCGCCGAAGCGGCCGTGAAGGTCGGCCTTCCCTATGTCTACACCGGTTCCTTCGACACTGCCGGCAA
AACGATGATGGGCCTGCACCCGAAGGACATTCACGGCGTCGCCGCCGACATCGGCGAAGGCCCGGTCGCCGTCGGCGCCA
ACTGCGGCGTCGGGGCGTCGGATATCCTCTCGTCCCTCCTCGACATGACGGCCGAAAGCCCGGATGCAACGATCGTCGTC
AAAGGCAATTGTGGAATTCCCGAGTTCCGCGGCTCCGAGATCCACTATTCGGGCACGCCGCCGCTGATGGCGGAATATGC
GCGGTTGGCCATGGACGCCGGCGCCAGGATCATCGGCGGCTGTTGCGGCACCTCCTGCAACCATTTGGCCGCAATGCGTC
TCGCGATCGACAATCACATCAAAGGTGAGCGCCCGACACTCGAGACGATCGTCGAGAAAATCGGTCCGTTGCGCAACAAG
TCTGCAAATGAAGGCCCCGCCGCCCCCGCTCGCGAGCGCCGCCGGCGCGCATAA

Upstream 100 bases:

>100_bases
GAGGCCAGAACGCATTTCAGCCTGATGCGCGCAATTCTGTTGCCAACACATAAAGATATCTTTATATCCTGTTACAGTTC
CATTCAGCCGGGAAAAACCG

Downstream 100 bases:

>100_bases
ATCACTGCTGGCAGGAGCTCCGGCAATAAAGCCGTAGCTCTCCTATTGCGCGCGTTCAACGCCAGTGCGTGAACGTGCTG
GGAAAACGTCGTTTGCGCTG

Product: methionine synthase I

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSVAPHALADLLAQKGVLLADGATGTSLFAMGLEAGEAPEIWNETKPENITKLHQDFVDAGADIILTNSFGGTRHRLKLH
QAEDRVHQLNKRAAEIARAVADKAPRKVITAGSVGPTGELLIPLGALSYEDAVAAFVEQIEGLKAGGAEVAWIETMSSAD
EIRAAAEAAVKVGLPYVYTGSFDTAGKTMMGLHPKDIHGVAADIGEGPVAVGANCGVGASDILSSLLDMTAESPDATIVV
KGNCGIPEFRGSEIHYSGTPPLMAEYARLAMDAGARIIGGCCGTSCNHLAAMRLAIDNHIKGERPTLETIVEKIGPLRNK
SANEGPAAPARERRRRA

Sequences:

>Translated_337_residues
MSVAPHALADLLAQKGVLLADGATGTSLFAMGLEAGEAPEIWNETKPENITKLHQDFVDAGADIILTNSFGGTRHRLKLH
QAEDRVHQLNKRAAEIARAVADKAPRKVITAGSVGPTGELLIPLGALSYEDAVAAFVEQIEGLKAGGAEVAWIETMSSAD
EIRAAAEAAVKVGLPYVYTGSFDTAGKTMMGLHPKDIHGVAADIGEGPVAVGANCGVGASDILSSLLDMTAESPDATIVV
KGNCGIPEFRGSEIHYSGTPPLMAEYARLAMDAGARIIGGCCGTSCNHLAAMRLAIDNHIKGERPTLETIVEKIGPLRNK
SANEGPAAPARERRRRA
>Mature_336_residues
SVAPHALADLLAQKGVLLADGATGTSLFAMGLEAGEAPEIWNETKPENITKLHQDFVDAGADIILTNSFGGTRHRLKLHQ
AEDRVHQLNKRAAEIARAVADKAPRKVITAGSVGPTGELLIPLGALSYEDAVAAFVEQIEGLKAGGAEVAWIETMSSADE
IRAAAEAAVKVGLPYVYTGSFDTAGKTMMGLHPKDIHGVAADIGEGPVAVGANCGVGASDILSSLLDMTAESPDATIVVK
GNCGIPEFRGSEIHYSGTPPLMAEYARLAMDAGARIIGGCCGTSCNHLAAMRLAIDNHIKGERPTLETIVEKIGPLRNKS
ANEGPAAPARERRRRA

Specific function: Terminal step in the de novo biosynthesis of methionine. [C]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=280, Percent_Identity=27.8571428571429, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1790450, Length=276, Percent_Identity=30.7971014492754, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1786456, Length=307, Percent_Identity=27.0358306188925, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=321, Percent_Identity=28.3489096573209, Blast_Score=119, Evalue=2e-27,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 35183; Mature: 35052

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAPHALADLLAQKGVLLADGATGTSLFAMGLEAGEAPEIWNETKPENITKLHQDFVDA
CCCCHHHHHHHHHHCCEEEECCCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GADIILTNSFGGTRHRLKLHQAEDRVHQLNKRAAEIARAVADKAPRKVITAGSVGPTGEL
CCCEEEECCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCE
LIPLGALSYEDAVAAFVEQIEGLKAGGAEVAWIETMSSADEIRAAAEAAVKVGLPYVYTG
EEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEC
SFDTAGKTMMGLHPKDIHGVAADIGEGPVAVGANCGVGASDILSSLLDMTAESPDATIVV
CCCCCCCCEECCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KGNCGIPEFRGSEIHYSGTPPLMAEYARLAMDAGARIIGGCCGTSCNHLAAMRLAIDNHI
ECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCC
KGERPTLETIVEKIGPLRNKSANEGPAAPARERRRRA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC
>Mature Secondary Structure 
SVAPHALADLLAQKGVLLADGATGTSLFAMGLEAGEAPEIWNETKPENITKLHQDFVDA
CCCHHHHHHHHHHCCEEEECCCCCHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHC
GADIILTNSFGGTRHRLKLHQAEDRVHQLNKRAAEIARAVADKAPRKVITAGSVGPTGEL
CCCEEEECCCCCCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCE
LIPLGALSYEDAVAAFVEQIEGLKAGGAEVAWIETMSSADEIRAAAEAAVKVGLPYVYTG
EEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEC
SFDTAGKTMMGLHPKDIHGVAADIGEGPVAVGANCGVGASDILSSLLDMTAESPDATIVV
CCCCCCCCEECCCCHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEE
KGNCGIPEFRGSEIHYSGTPPLMAEYARLAMDAGARIIGGCCGTSCNHLAAMRLAIDNHI
ECCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCC
KGERPTLETIVEKIGPLRNKSANEGPAAPARERRRRA
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]