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The map label for this gene is cobQ [H]

Identifier: 150396968

GI number: 150396968

Start: 1848719

End: 1850173

Strand: Reverse

Name: cobQ [H]

Synonym: Smed_1765

Alternate gene names: 150396968

Gene position: 1850173-1848719 (Counterclockwise)

Preceding gene: 150396972

Following gene: 150396967

Centisome position: 48.92

GC content: 63.37

Gene sequence:

>1455_bases
ATGACACGGAAGATCATGCTGCAGGGAACCGGCTCGGATGTCGGAAAATCGGTACTTGTCGCGGGCCTTTGCAGGCTCGC
TTCAAACCGGGGGCTCAGCGTAAAGCCCTTCAAGCCCCAGAACATGTCGAACAACGCCGCCGTTTCCGACGACGGCGGCG
AAATCGGCCGTGCGCAATGGCTCCAGGCGCTTGCCGCGCGCGTGCCGTCCTCCGTTCACATGAACCCCGTGCTGCTCAAA
CCGCAATCGGACGTGGGCAGCCAGGTCGTCGTCCAGGGAAGGGTGGCGGGGCAGGCCAGGGGGAAGGAATACCAGGCTCT
GAAGCCGGGTCTCCTCGGTTCCGTCATGGAGAGTTTCGAGCTGGTCTCTGCCGGTGCCGATCTCGTCATTGTCGAGGGGG
CGGGCTCACCCGCCGAGATCAATCTGCGCGCGGGCGACATCGCCAATATGGGGTTTGCGACCCGTGCCGGCGTGCCTGTC
GTTCTGGTCGGAGATATCGATCGTGGCGGCGTAATCGCGTCTCTGGTCGGTACGCATGCGATCCTGTCGGATGAGGATCG
GCGAATGGTCACCGGCTATTTGATCAACAAGTTCCGCGGCGACGTGACGCTGTTCGACGACGGCATAGCATCGATCCGTA
CCTTTACCGGCTGGCCCTGTTTCGGGGTCGTGCCGTGGCTTAAGAGCGCGGGGCGGCTGCCTGCCGAAGACTCCGTCGTG
CTCGAGAGACTGGCGCGGGGCGGTGGCAAGGCGCTGAAGGTCGCCGTGCCCGTTCTGTCCCGAATCGCAAATTTCGACGA
CCTCGACCCTTTGGCCGCAGAACCGGATGTGGAGATCGTCTTTGTCCGGCCGGGTACGCCGCTTCCCGACGACGCGGCCC
TGGTCGTGATACCCGGTTCCAAATCCACCATCGCCGATCTCGAAGACTTCCGCAGGCAGGGCTGGGACCGGGACCTTGAT
CGGCACATCCGGCGCGGTGGCCGGGTCATCGGAATCTGCGGCGGCTACCAGATGCTCGGAACACGAGTCATCGACCCGCT
CGGTATCGAAGGCGGCAAGCGAGAGATCGAAGGCTTGGGGCTGCTTTCTGTCGAGACGGAGATGGCGCCCGAAAAAACCG
TGCGCAACAGCCGTGCCTGGTCGCGGGAATACGGCGTGTTACTCGAAGGTTACGAGATCCATCTCGGCAGAACGGCAGGG
ATCGACTGCGGTCGTGCTCCGGTTGAGATCGACGGTCGTCGTGATGGAGCGATGTCGGCGGACGGCCGCGTCATGGGAAC
CTATCTCCACGGACTTTTCGGAAGCGACCCCTACAGGGCCGAACTGCTCAGGAGCTTCGGAATCGAAGGCGGCGGAGGCA
ATTACCGCCAGTCGGTGGATGCGGCTCTCGACGAGATCGCCGCAGAACTCGACGCGGTACTCGACCGGGTGTGGCTCGAG
ACATTGCTGGGTTAG

Upstream 100 bases:

>100_bases
GAAGGTCGGAAACGAGCCGTGATCGCCAGTGCCGCATTGGTGGCGGAAACGCGTCGGTACAGAGCTTTGCGGCACTCGGT
TTCGATGCTACGGGGGAGGC

Downstream 100 bases:

>100_bases
CGCCGAAGGCAAGGCGCCCGCCTGTTCGGAGCCTCCCGGTGGGGCACCCTTGGCGCTGTCGGCACAGCGCCTACACGAAG
GAAGGTTTGAAATGCATTAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTRKIMLQGTGSDVGKSVLVAGLCRLASNRGLSVKPFKPQNMSNNAAVSDDGGEIGRAQWLQALAARVPSSVHMNPVLLK
PQSDVGSQVVVQGRVAGQARGKEYQALKPGLLGSVMESFELVSAGADLVIVEGAGSPAEINLRAGDIANMGFATRAGVPV
VLVGDIDRGGVIASLVGTHAILSDEDRRMVTGYLINKFRGDVTLFDDGIASIRTFTGWPCFGVVPWLKSAGRLPAEDSVV
LERLARGGGKALKVAVPVLSRIANFDDLDPLAAEPDVEIVFVRPGTPLPDDAALVVIPGSKSTIADLEDFRRQGWDRDLD
RHIRRGGRVIGICGGYQMLGTRVIDPLGIEGGKREIEGLGLLSVETEMAPEKTVRNSRAWSREYGVLLEGYEIHLGRTAG
IDCGRAPVEIDGRRDGAMSADGRVMGTYLHGLFGSDPYRAELLRSFGIEGGGGNYRQSVDAALDEIAAELDAVLDRVWLE
TLLG

Sequences:

>Translated_484_residues
MTRKIMLQGTGSDVGKSVLVAGLCRLASNRGLSVKPFKPQNMSNNAAVSDDGGEIGRAQWLQALAARVPSSVHMNPVLLK
PQSDVGSQVVVQGRVAGQARGKEYQALKPGLLGSVMESFELVSAGADLVIVEGAGSPAEINLRAGDIANMGFATRAGVPV
VLVGDIDRGGVIASLVGTHAILSDEDRRMVTGYLINKFRGDVTLFDDGIASIRTFTGWPCFGVVPWLKSAGRLPAEDSVV
LERLARGGGKALKVAVPVLSRIANFDDLDPLAAEPDVEIVFVRPGTPLPDDAALVVIPGSKSTIADLEDFRRQGWDRDLD
RHIRRGGRVIGICGGYQMLGTRVIDPLGIEGGKREIEGLGLLSVETEMAPEKTVRNSRAWSREYGVLLEGYEIHLGRTAG
IDCGRAPVEIDGRRDGAMSADGRVMGTYLHGLFGSDPYRAELLRSFGIEGGGGNYRQSVDAALDEIAAELDAVLDRVWLE
TLLG
>Mature_483_residues
TRKIMLQGTGSDVGKSVLVAGLCRLASNRGLSVKPFKPQNMSNNAAVSDDGGEIGRAQWLQALAARVPSSVHMNPVLLKP
QSDVGSQVVVQGRVAGQARGKEYQALKPGLLGSVMESFELVSAGADLVIVEGAGSPAEINLRAGDIANMGFATRAGVPVV
LVGDIDRGGVIASLVGTHAILSDEDRRMVTGYLINKFRGDVTLFDDGIASIRTFTGWPCFGVVPWLKSAGRLPAEDSVVL
ERLARGGGKALKVAVPVLSRIANFDDLDPLAAEPDVEIVFVRPGTPLPDDAALVVIPGSKSTIADLEDFRRQGWDRDLDR
HIRRGGRVIGICGGYQMLGTRVIDPLGIEGGKREIEGLGLLSVETEMAPEKTVRNSRAWSREYGVLLEGYEIHLGRTAGI
DCGRAPVEIDGRRDGAMSADGRVMGTYLHGLFGSDPYRAELLRSFGIEGGGGNYRQSVDAALDEIAAELDAVLDRVWLET
LLG

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 51487; Mature: 51355

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKIMLQGTGSDVGKSVLVAGLCRLASNRGLSVKPFKPQNMSNNAAVSDDGGEIGRAQW
CCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCHHHH
LQALAARVPSSVHMNPVLLKPQSDVGSQVVVQGRVAGQARGKEYQALKPGLLGSVMESFE
HHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHH
LVSAGADLVIVEGAGSPAEINLRAGDIANMGFATRAGVPVVLVGDIDRGGVIASLVGTHA
HHHCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHH
ILSDEDRRMVTGYLINKFRGDVTLFDDGIASIRTFTGWPCFGVVPWLKSAGRLPAEDSVV
HCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHH
LERLARGGGKALKVAVPVLSRIANFDDLDPLAAEPDVEIVFVRPGTPLPDDAALVVIPGS
HHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCC
KSTIADLEDFRRQGWDRDLDRHIRRGGRVIGICGGYQMLGTRVIDPLGIEGGKREIEGLG
CCHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHCCCE
LLSVETEMAPEKTVRNSRAWSREYGVLLEGYEIHLGRTAGIDCGRAPVEIDGRRDGAMSA
EEEEECCCCCHHHHHCCHHHHHHCCEEEECEEEEECCCCCCCCCCCCEEECCCCCCCCCC
DGRVMGTYLHGLFGSDPYRAELLRSFGIEGGGGNYRQSVDAALDEIAAELDAVLDRVWLE
CCEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLG
HHCC
>Mature Secondary Structure 
TRKIMLQGTGSDVGKSVLVAGLCRLASNRGLSVKPFKPQNMSNNAAVSDDGGEIGRAQW
CCEEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCEECCCCCCCCHHHH
LQALAARVPSSVHMNPVLLKPQSDVGSQVVVQGRVAGQARGKEYQALKPGLLGSVMESFE
HHHHHHHCCCCCCCCEEEECCCCCCCCEEEEEEEECCCCCCCCCHHCCCHHHHHHHHHHH
LVSAGADLVIVEGAGSPAEINLRAGDIANMGFATRAGVPVVLVGDIDRGGVIASLVGTHA
HHHCCCCEEEEECCCCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCCCHHHHHHHHHH
ILSDEDRRMVTGYLINKFRGDVTLFDDGIASIRTFTGWPCFGVVPWLKSAGRLPAEDSVV
HCCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHHCCCCCHHHHHHHHHHCCCCCCCHHHH
LERLARGGGKALKVAVPVLSRIANFDDLDPLAAEPDVEIVFVRPGTPLPDDAALVVIPGS
HHHHHCCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCC
KSTIADLEDFRRQGWDRDLDRHIRRGGRVIGICGGYQMLGTRVIDPLGIEGGKREIEGLG
CCHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHCCCE
LLSVETEMAPEKTVRNSRAWSREYGVLLEGYEIHLGRTAGIDCGRAPVEIDGRRDGAMSA
EEEEECCCCCHHHHHCCHHHHHHCCEEEECEEEEECCCCCCCCCCCCEEECCCCCCCCCC
DGRVMGTYLHGLFGSDPYRAELLRSFGIEGGGGNYRQSVDAALDEIAAELDAVLDRVWLE
CCEEHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TLLG
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]