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The map label for this gene is bpoA2 [H]

Identifier: 150396967

GI number: 150396967

Start: 1847812

End: 1848627

Strand: Reverse

Name: bpoA2 [H]

Synonym: Smed_1764

Alternate gene names: 150396967

Gene position: 1848627-1847812 (Counterclockwise)

Preceding gene: 150396968

Following gene: 150396966

Centisome position: 48.88

GC content: 61.64

Gene sequence:

>816_bases
ATGCATTATTGCGACGACGATCTTCGGCGTTTCGAAGCGGAGGGGGCGGCGGCTCTGCCTCCTGCCGCGGAACAGGGCTT
TGTCGAGCACGATGGCGCCCGAATATGGTTTTCGATATACGGCGCCGGAACACCGGTGCTCCTCCTGCACGGCGGCCTCG
GCCACAGCGGCAACTGGGGCTATCAGCTTGCGCCGCTTCTCGAGGCGGGCCACCGCGTCGTGCTTGTCGACAGTCGCGGA
CATGGGCGCAGTACGCGCGATAAGCGGCCTTACAGCTATGAATTGATGGCATCCGATGTGCTTGCGGTCATGGACAGGCT
GCAGCTCCACAACGCGGCGTTGGTAGGCTGGAGCGACGGGGCATGCATCAGCCTCGTTCTTGCAAGGCAGGCTCCCGCGC
GGATTGCCGGCGTCTTTTTCTTCGCCTGCAACATGGACCCGAGCGGCACAAAGGCGTTCGAGGCGACACCCGTCATCGAC
CGTTGCTTCGCCCGCCACCGGAAGGACTACGCCGAATTATCGGCCACGCCGGATGATTTCGATGACTTTGTCGCGGACGT
CGGCGAGATGATGCGGACGCAACCCGATTATTCGGCAGCCGATCTTTCCGCGATCCGCGTGCCTGTCGCGATCGTGCATG
CAGAGAAGGACGAGTTCATAAAAATGGAGCACGCCGAGTATCTCGCCCGGAGCATTCCCTCGGCCGAACTGATTCCGCTT
CAAGGCGTCAGCCACTTTGCTCCGCTGCAGAGACCGGAAGTGTTCAATGGTGCCATGCTTGCGTTCCTGGAGCGAATACG
TCCCGCGGGTAGTTAG

Upstream 100 bases:

>100_bases
CTGGGTTAGCGCCGAAGGCAAGGCGCCCGCCTGTTCGGAGCCTCCCGGTGGGGCACCCTTGGCGCTGTCGGCACAGCGCC
TACACGAAGGAAGGTTTGAA

Downstream 100 bases:

>100_bases
AGCATTTCCGCTTTTTTCCGAATCGCGGAAACTCTCTAATCCTTTACTTTGCCGCAAATCCGGACGGAAAACCGCTACGC
ACTTTTCCTGGAATTGTTTT

Product: alpha/beta hydrolase fold protein

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: BPO2; Bromide peroxidase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MHYCDDDLRRFEAEGAAALPPAAEQGFVEHDGARIWFSIYGAGTPVLLLHGGLGHSGNWGYQLAPLLEAGHRVVLVDSRG
HGRSTRDKRPYSYELMASDVLAVMDRLQLHNAALVGWSDGACISLVLARQAPARIAGVFFFACNMDPSGTKAFEATPVID
RCFARHRKDYAELSATPDDFDDFVADVGEMMRTQPDYSAADLSAIRVPVAIVHAEKDEFIKMEHAEYLARSIPSAELIPL
QGVSHFAPLQRPEVFNGAMLAFLERIRPAGS

Sequences:

>Translated_271_residues
MHYCDDDLRRFEAEGAAALPPAAEQGFVEHDGARIWFSIYGAGTPVLLLHGGLGHSGNWGYQLAPLLEAGHRVVLVDSRG
HGRSTRDKRPYSYELMASDVLAVMDRLQLHNAALVGWSDGACISLVLARQAPARIAGVFFFACNMDPSGTKAFEATPVID
RCFARHRKDYAELSATPDDFDDFVADVGEMMRTQPDYSAADLSAIRVPVAIVHAEKDEFIKMEHAEYLARSIPSAELIPL
QGVSHFAPLQRPEVFNGAMLAFLERIRPAGS
>Mature_271_residues
MHYCDDDLRRFEAEGAAALPPAAEQGFVEHDGARIWFSIYGAGTPVLLLHGGLGHSGNWGYQLAPLLEAGHRVVLVDSRG
HGRSTRDKRPYSYELMASDVLAVMDRLQLHNAALVGWSDGACISLVLARQAPARIAGVFFFACNMDPSGTKAFEATPVID
RCFARHRKDYAELSATPDDFDDFVADVGEMMRTQPDYSAADLSAIRVPVAIVHAEKDEFIKMEHAEYLARSIPSAELIPL
QGVSHFAPLQRPEVFNGAMLAFLERIRPAGS

Specific function: May be a chlorinating enzyme involved in 7- chlorotetracycline biosynthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial non-heme bromo- and chloro- peroxidases family [H]

Homologues:

Organism=Homo sapiens, GI221316588, Length=264, Percent_Identity=25, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 29710; Mature: 29710

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHYCDDDLRRFEAEGAAALPPAAEQGFVEHDGARIWFSIYGAGTPVLLLHGGLGHSGNWG
CCCCCHHHHHHHCCCCCCCCCCHHCCCEECCCCEEEEEEECCCCCEEEEECCCCCCCCCC
YQLAPLLEAGHRVVLVDSRGHGRSTRDKRPYSYELMASDVLAVMDRLQLHNAALVGWSDG
EEECHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCC
ACISLVLARQAPARIAGVFFFACNMDPSGTKAFEATPVIDRCFARHRKDYAELSATPDDF
HHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCH
DDFVADVGEMMRTQPDYSAADLSAIRVPVAIVHAEKDEFIKMEHAEYLARSIPSAELIPL
HHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEC
QGVSHFAPLQRPEVFNGAMLAFLERIRPAGS
CCCHHHCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHYCDDDLRRFEAEGAAALPPAAEQGFVEHDGARIWFSIYGAGTPVLLLHGGLGHSGNWG
CCCCCHHHHHHHCCCCCCCCCCHHCCCEECCCCEEEEEEECCCCCEEEEECCCCCCCCCC
YQLAPLLEAGHRVVLVDSRGHGRSTRDKRPYSYELMASDVLAVMDRLQLHNAALVGWSDG
EEECHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECCCC
ACISLVLARQAPARIAGVFFFACNMDPSGTKAFEATPVIDRCFARHRKDYAELSATPDDF
HHHHHHHHHCCCHHEEEEEEEEECCCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCH
DDFVADVGEMMRTQPDYSAADLSAIRVPVAIVHAEKDEFIKMEHAEYLARSIPSAELIPL
HHHHHHHHHHHHCCCCCCHHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCEEEC
QGVSHFAPLQRPEVFNGAMLAFLERIRPAGS
CCCHHHCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1527491; 1783900; 7664081; 9642069 [H]