Click here to switch to the map view.

The map label for this gene is dppA [H]

Identifier: 150396954

GI number: 150396954

Start: 1831471

End: 1833087

Strand: Reverse

Name: dppA [H]

Synonym: Smed_1751

Alternate gene names: 150396954

Gene position: 1833087-1831471 (Counterclockwise)

Preceding gene: 150396955

Following gene: 150396953

Centisome position: 48.47

GC content: 59.55

Gene sequence:

>1617_bases
ATGAGGAACTTGTTACTGGCCGGCGTCTGCGCCGCCGCACTGATGGGAAATCCCGCATTCGCCGACGACATCAAGCAGGG
TGGCGAAATGACCGTCACCTATAAGGACGATGTTTCGACACTCGATCCGGCGATCGGCTACGACTGGCAGAACTGGTCGA
TGATCAAGTCGCTGTTCGACGGCCTGATGGATTATGTCCCGGGCACGACCGAGTTGCGTCCCGATCTTGCCGAAGCCTAT
GAAATCTCCGGGGACGGCAAAATCTTCACGTTCAAGCTGCGCCAGGGCGTCAAGTTTCACAATGGTCGTGAGCTGACTGC
CGAGGACGTGAAATATTCGATTGAGCGCGTGGTGAATCCGACGACCCAGAGCCCGGGTGCCGGGTTCTTCTCATCGATCA
AAGGCTTCGAAGATGTCTCGGCCGGAAAGGGGGGTGATCTGTCCGGCATCGCCGTGCAGGATCCGCACACAATCAGGTTC
GAACTGAGCCGGCCGGACGCCACCTTCCTCCACGTCATGGCGCTCAACTTTGCCCATGTCGTGCCAAAGGAAGAGGTCGA
GAAACACGGTGCGGATTTCGGGAAAAATCCCGTCGGTTCCGGAGCGTTCAAGCTTGCCGAGTGGACGCTTGGGCAACGCC
TGGTGTTCGAACGCTTCGCCGACTACTGGAACGAAGGGCTTCCGAAGCTTGACCGCATTACCTTCGAGGTCGGCCAGGAG
CCCGTTGTCGCGCTCCTTCGCCTGCAGAACGGCGAAATCGACGTGCCCGGAGACGGCATTCCGCCGGCGAAGTTCGTCGA
GGTGACCAAAGATCCTAATTTCAAGGAGCTGATCATTCAGGGCGGTCAGTTGCACACCGGCTATGTGACGATGAACGTCA
AGATGGCCCCCTTCGACAAGGTCGAGGTGCGCAAGGCTGTGAACATGGCCATCAACAAGGATCGCATCCTGCGCATCATC
AACGGTCGCGCAGTCGCCGCCAATCAGCCGCTGCCGCCCTCGATGCCGGGATATGCCAAGGATTATAAAGGATATGCCTA
TGATCCCGAGGGCGCCAAGAAGCTGCTCGAACAGGCCGGCCTGGGCGACGGGTTCTCGACCGAACTCTATGTCATGAACA
CCGACCCTCAGCCGCGTATCGCCCAGGCCATCCAGCAGGACCTGAAGGCGATCGGCATCACGGCATCGATAAAGTCGCTG
GCACAGGCCAATGTCATCGCGGCGGGCGGCGAGGAGAACCAGGCGCCGATGGTCTGGTCGGGCGGCATGGCATGGATTGC
CGACTTCCCGGATCCCTCGAATTTCTACGGCCCCATTCTGGGGTGCGGCGGTGCCGTGCCGGGAGGCTGGAACTGGTCCT
GGTACTGCAATGAGGAGCTCGACAAGAAGGCAGCCGAAGCCGATGCCATCGTAGACCCGGCAAAGGCCGCCGAGCGCGAG
GCCATGTGGCGCGACATCTATGTGAAGATCATGGAGGACGCACCCTGGGCACCGATCTTCAACGAGGAGCGCTTTACCAT
TCGCTCGGAGCGTATCGGCGGCGACGACAAGCTGTTCGTCGATCCGGTCCACATTCCCGTTCACTACGATCAGGTATATG
CAAAAGATGTGCAGTAA

Upstream 100 bases:

>100_bases
GGCAATCTGCTCGCCGACCTCATCACGCCGCTTATCGACCCGCGCATCAAGCTGCGCTGACGCGGGAAGAACAAACTGCA
GCAACAGAAAGGAACTTCAG

Downstream 100 bases:

>100_bases
CTGCGACTACACCATCCATGGACGCCATCACCACTACGGCTGGGATCGTTCGATCCCGCCGGCGGAGACCGTGGCGTCCG
GATCGAGGCTCGAGTTCAAC

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRNLLLAGVCAAALMGNPAFADDIKQGGEMTVTYKDDVSTLDPAIGYDWQNWSMIKSLFDGLMDYVPGTTELRPDLAEAY
EISGDGKIFTFKLRQGVKFHNGRELTAEDVKYSIERVVNPTTQSPGAGFFSSIKGFEDVSAGKGGDLSGIAVQDPHTIRF
ELSRPDATFLHVMALNFAHVVPKEEVEKHGADFGKNPVGSGAFKLAEWTLGQRLVFERFADYWNEGLPKLDRITFEVGQE
PVVALLRLQNGEIDVPGDGIPPAKFVEVTKDPNFKELIIQGGQLHTGYVTMNVKMAPFDKVEVRKAVNMAINKDRILRII
NGRAVAANQPLPPSMPGYAKDYKGYAYDPEGAKKLLEQAGLGDGFSTELYVMNTDPQPRIAQAIQQDLKAIGITASIKSL
AQANVIAAGGEENQAPMVWSGGMAWIADFPDPSNFYGPILGCGGAVPGGWNWSWYCNEELDKKAAEADAIVDPAKAAERE
AMWRDIYVKIMEDAPWAPIFNEERFTIRSERIGGDDKLFVDPVHIPVHYDQVYAKDVQ

Sequences:

>Translated_538_residues
MRNLLLAGVCAAALMGNPAFADDIKQGGEMTVTYKDDVSTLDPAIGYDWQNWSMIKSLFDGLMDYVPGTTELRPDLAEAY
EISGDGKIFTFKLRQGVKFHNGRELTAEDVKYSIERVVNPTTQSPGAGFFSSIKGFEDVSAGKGGDLSGIAVQDPHTIRF
ELSRPDATFLHVMALNFAHVVPKEEVEKHGADFGKNPVGSGAFKLAEWTLGQRLVFERFADYWNEGLPKLDRITFEVGQE
PVVALLRLQNGEIDVPGDGIPPAKFVEVTKDPNFKELIIQGGQLHTGYVTMNVKMAPFDKVEVRKAVNMAINKDRILRII
NGRAVAANQPLPPSMPGYAKDYKGYAYDPEGAKKLLEQAGLGDGFSTELYVMNTDPQPRIAQAIQQDLKAIGITASIKSL
AQANVIAAGGEENQAPMVWSGGMAWIADFPDPSNFYGPILGCGGAVPGGWNWSWYCNEELDKKAAEADAIVDPAKAAERE
AMWRDIYVKIMEDAPWAPIFNEERFTIRSERIGGDDKLFVDPVHIPVHYDQVYAKDVQ
>Mature_538_residues
MRNLLLAGVCAAALMGNPAFADDIKQGGEMTVTYKDDVSTLDPAIGYDWQNWSMIKSLFDGLMDYVPGTTELRPDLAEAY
EISGDGKIFTFKLRQGVKFHNGRELTAEDVKYSIERVVNPTTQSPGAGFFSSIKGFEDVSAGKGGDLSGIAVQDPHTIRF
ELSRPDATFLHVMALNFAHVVPKEEVEKHGADFGKNPVGSGAFKLAEWTLGQRLVFERFADYWNEGLPKLDRITFEVGQE
PVVALLRLQNGEIDVPGDGIPPAKFVEVTKDPNFKELIIQGGQLHTGYVTMNVKMAPFDKVEVRKAVNMAINKDRILRII
NGRAVAANQPLPPSMPGYAKDYKGYAYDPEGAKKLLEQAGLGDGFSTELYVMNTDPQPRIAQAIQQDLKAIGITASIKSL
AQANVIAAGGEENQAPMVWSGGMAWIADFPDPSNFYGPILGCGGAVPGGWNWSWYCNEELDKKAAEADAIVDPAKAAERE
AMWRDIYVKIMEDAPWAPIFNEERFTIRSERIGGDDKLFVDPVHIPVHYDQVYAKDVQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=495, Percent_Identity=31.5151515151515, Blast_Score=219, Evalue=3e-58,
Organism=Escherichia coli, GI1787052, Length=519, Percent_Identity=29.4797687861272, Blast_Score=218, Evalue=9e-58,
Organism=Escherichia coli, GI1787495, Length=526, Percent_Identity=27.3764258555133, Blast_Score=155, Evalue=8e-39,
Organism=Escherichia coli, GI1787551, Length=495, Percent_Identity=26.4646464646465, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1787762, Length=489, Percent_Identity=26.7893660531697, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789397, Length=556, Percent_Identity=25.8992805755396, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081878, Length=522, Percent_Identity=26.4367816091954, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789887, Length=522, Percent_Identity=25.6704980842912, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87082063, Length=273, Percent_Identity=23.4432234432234, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59159; Mature: 59159

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNLLLAGVCAAALMGNPAFADDIKQGGEMTVTYKDDVSTLDPAIGYDWQNWSMIKSLFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCHHHHHHHHH
GLMDYVPGTTELRPDLAEAYEISGDGKIFTFKLRQGVKFHNGRELTAEDVKYSIERVVNP
HHHHHCCCCCCCCCCHHHHEEECCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCC
TTQSPGAGFFSSIKGFEDVSAGKGGDLSGIAVQDPHTIRFELSRPDATFLHVMALNFAHV
CCCCCCCCHHHHHCCCHHCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHC
VPKEEVEKHGADFGKNPVGSGAFKLAEWTLGQRLVFERFADYWNEGLPKLDRITFEVGQE
CCHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
PVVALLRLQNGEIDVPGDGIPPAKFVEVTKDPNFKELIIQGGQLHTGYVTMNVKMAPFDK
CEEEHEEECCCCEECCCCCCCCHHEEEECCCCCHHHHEEECCEEEEEEEEEEEEECCCCH
VEVRKAVNMAINKDRILRIINGRAVAANQPLPPSMPGYAKDYKGYAYDPEGAKKLLEQAG
HHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCC
LGDGFSTELYVMNTDPQPRIAQAIQQDLKAIGITASIKSLAQANVIAAGGEENQAPMVWS
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCCEEEE
GGMAWIADFPDPSNFYGPILGCGGAVPGGWNWSWYCNEELDKKAAEADAIVDPAKAAERE
CCEEEEECCCCCCCCCCCHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHH
AMWRDIYVKIMEDAPWAPIFNEERFTIRSERIGGDDKLFVDPVHIPVHYDQVYAKDVQ
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEHHHHHHCCCC
>Mature Secondary Structure
MRNLLLAGVCAAALMGNPAFADDIKQGGEMTVTYKDDVSTLDPAIGYDWQNWSMIKSLFD
CCCHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCHHHHHHHHH
GLMDYVPGTTELRPDLAEAYEISGDGKIFTFKLRQGVKFHNGRELTAEDVKYSIERVVNP
HHHHHCCCCCCCCCCHHHHEEECCCCEEEEEEECCCCEECCCCCCCHHHHHHHHHHHCCC
TTQSPGAGFFSSIKGFEDVSAGKGGDLSGIAVQDPHTIRFELSRPDATFLHVMALNFAHV
CCCCCCCCHHHHHCCCHHCCCCCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHC
VPKEEVEKHGADFGKNPVGSGAFKLAEWTLGQRLVFERFADYWNEGLPKLDRITFEVGQE
CCHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
PVVALLRLQNGEIDVPGDGIPPAKFVEVTKDPNFKELIIQGGQLHTGYVTMNVKMAPFDK
CEEEHEEECCCCEECCCCCCCCHHEEEECCCCCHHHHEEECCEEEEEEEEEEEEECCCCH
VEVRKAVNMAINKDRILRIINGRAVAANQPLPPSMPGYAKDYKGYAYDPEGAKKLLEQAG
HHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCHHCCCCCCCCCHHHHHHHHHCC
LGDGFSTELYVMNTDPQPRIAQAIQQDLKAIGITASIKSLAQANVIAAGGEENQAPMVWS
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCCEEEE
GGMAWIADFPDPSNFYGPILGCGGAVPGGWNWSWYCNEELDKKAAEADAIVDPAKAAERE
CCEEEEECCCCCCCCCCCHHCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHHHH
AMWRDIYVKIMEDAPWAPIFNEERFTIRSERIGGDDKLFVDPVHIPVHYDQVYAKDVQ
HHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]