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The map label for this gene is cobD [H]

Identifier: 150396946

GI number: 150396946

Start: 1822851

End: 1823804

Strand: Reverse

Name: cobD [H]

Synonym: Smed_1743

Alternate gene names: 150396946

Gene position: 1823804-1822851 (Counterclockwise)

Preceding gene: 150396947

Following gene: 150396944

Centisome position: 48.22

GC content: 61.43

Gene sequence:

>954_bases
ATGGCACTGCTTTTGGATCGCCTCATTGGCGATCCGGACTGGCTCTGGTCGCGGATCACGCATCCCGTGGTGTTTTTCGG
CCAGGCGATCGGGTTCGTCGAAAAGGCCCTGAACACCGGAGAGGTTGCCAATACCTGGCTGAAGGTGCGCGGCGCGGTCG
GTATTCTGATGCTGCTCGCCGCAGCCATCGGCTCAGGTATCGTGCTTGCCCGGCTCTTCGATGTGCTTGGCGCCCTCGGC
TCGTTGCTCGAAGTTGTCACCGTTGCCATCTTCCTCGCGCAAAAGAGCCTCGCGGATCACGTTTCCAGGGTTGCCGTCGG
TTTACGTCGCGGTGGAGTTTCGGGCGGCCGCAAAGAAGTGTCCATGATCGTCGGACGCGATCCGAGCACGCTCGACGAGC
CCGGGATATGCCGCGCAGCAATCGAAAGCCTGGCTGAGAATTTTTCGGACGGCGTCGTTGCTCCGGCATTCTGGTATGCA
ATTGCTGGTCTGCCCGGTCTTCTGGCCTACAAGATGCTGAACACGGCCGATTCGATGATTGGCCACAGAAGCCCGAAATA
TCTTCGCTTCGGTTGGGCATCGGCGCGGCTCGACGATCTGGCGAACCTGCCCGCCGCAAGGCTCTCGGCACTCCTGATCG
CCGCCGGGGCCTATTTCAAGTGCGGTGAAGAAGAGGCAAAAAACGCGATACGGGTGGCGCGGCGCGATCACGGGTTTCAC
CGTTCGCCAAATTCCGGATGGCCGGAGGCCGCGATGGCCGGCGCCATCGGCATTCAGCTTGCCGGTCCGAGAGTCTATGG
CGGCGTAAAGGTGGACGAGCCCATGATGAACGCCGCCGGTCGTGCGGCCGCAGCCATCGAGGATATCGACGCTGCCGTCA
CTGTCTTCTATGCGGCCTGCCTGGTGATGACGTCTGCCTTTGCCTTCGGTTTTCTGCTTCTGGCGGCTCTATAG

Upstream 100 bases:

>100_bases
AATCGGGCTGTCACCGAACCCGGCAAGCGACCGCCGGCTCGCAGAGGCGTTTAATCGAATGGGTTTTTAAGTGTCGTCAG
AAATACTTCTTCTTCTCGTC

Downstream 100 bases:

>100_bases
CGATCCGAGTGCCGTCGTGGCCTGCTGCCTGCAATGATGACGCTTACGGATGCACTCAACTGTCGATGCTGGCATTCTTC
ATCAGCCGGCGGATATCCGG

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MALLLDRLIGDPDWLWSRITHPVVFFGQAIGFVEKALNTGEVANTWLKVRGAVGILMLLAAAIGSGIVLARLFDVLGALG
SLLEVVTVAIFLAQKSLADHVSRVAVGLRRGGVSGGRKEVSMIVGRDPSTLDEPGICRAAIESLAENFSDGVVAPAFWYA
IAGLPGLLAYKMLNTADSMIGHRSPKYLRFGWASARLDDLANLPAARLSALLIAAGAYFKCGEEEAKNAIRVARRDHGFH
RSPNSGWPEAAMAGAIGIQLAGPRVYGGVKVDEPMMNAAGRAAAAIEDIDAAVTVFYAACLVMTSAFAFGFLLLAAL

Sequences:

>Translated_317_residues
MALLLDRLIGDPDWLWSRITHPVVFFGQAIGFVEKALNTGEVANTWLKVRGAVGILMLLAAAIGSGIVLARLFDVLGALG
SLLEVVTVAIFLAQKSLADHVSRVAVGLRRGGVSGGRKEVSMIVGRDPSTLDEPGICRAAIESLAENFSDGVVAPAFWYA
IAGLPGLLAYKMLNTADSMIGHRSPKYLRFGWASARLDDLANLPAARLSALLIAAGAYFKCGEEEAKNAIRVARRDHGFH
RSPNSGWPEAAMAGAIGIQLAGPRVYGGVKVDEPMMNAAGRAAAAIEDIDAAVTVFYAACLVMTSAFAFGFLLLAAL
>Mature_316_residues
ALLLDRLIGDPDWLWSRITHPVVFFGQAIGFVEKALNTGEVANTWLKVRGAVGILMLLAAAIGSGIVLARLFDVLGALGS
LLEVVTVAIFLAQKSLADHVSRVAVGLRRGGVSGGRKEVSMIVGRDPSTLDEPGICRAAIESLAENFSDGVVAPAFWYAI
AGLPGLLAYKMLNTADSMIGHRSPKYLRFGWASARLDDLANLPAARLSALLIAAGAYFKCGEEEAKNAIRVARRDHGFHR
SPNSGWPEAAMAGAIGIQLAGPRVYGGVKVDEPMMNAAGRAAAAIEDIDAAVTVFYAACLVMTSAFAFGFLLLAAL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 33420; Mature: 33289

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLLDRLIGDPDWLWSRITHPVVFFGQAIGFVEKALNTGEVANTWLKVRGAVGILMLLA
CHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AAIGSGIVLARLFDVLGALGSLLEVVTVAIFLAQKSLADHVSRVAVGLRRGGVSGGRKEV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SMIVGRDPSTLDEPGICRAAIESLAENFSDGVVAPAFWYAIAGLPGLLAYKMLNTADSMI
HHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GHRSPKYLRFGWASARLDDLANLPAARLSALLIAAGAYFKCGEEEAKNAIRVARRDHGFH
CCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCC
RSPNSGWPEAAMAGAIGIQLAGPRVYGGVKVDEPMMNAAGRAAAAIEDIDAAVTVFYAAC
CCCCCCCCHHHHHHHHHEEECCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
LVMTSAFAFGFLLLAAL
HHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
ALLLDRLIGDPDWLWSRITHPVVFFGQAIGFVEKALNTGEVANTWLKVRGAVGILMLLA
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AAIGSGIVLARLFDVLGALGSLLEVVTVAIFLAQKSLADHVSRVAVGLRRGGVSGGRKEV
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SMIVGRDPSTLDEPGICRAAIESLAENFSDGVVAPAFWYAIAGLPGLLAYKMLNTADSMI
HHEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHH
GHRSPKYLRFGWASARLDDLANLPAARLSALLIAAGAYFKCGEEEAKNAIRVARRDHGFH
CCCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCC
RSPNSGWPEAAMAGAIGIQLAGPRVYGGVKVDEPMMNAAGRAAAAIEDIDAAVTVFYAAC
CCCCCCCCHHHHHHHHHEEECCCEEECCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
LVMTSAFAFGFLLLAAL
HHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]