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The map label for this gene is artJ [H]

Identifier: 150396797

GI number: 150396797

Start: 1686274

End: 1687056

Strand: Direct

Name: artJ [H]

Synonym: Smed_1588

Alternate gene names: 150396797

Gene position: 1686274-1687056 (Clockwise)

Preceding gene: 150396794

Following gene: 150396798

Centisome position: 44.59

GC content: 58.24

Gene sequence:

>783_bases
ATGCTTATTTCCAAACGGCTTGCGGCCACCGCATCGGCTGCCGTTTTCGTGCTTATGACCGGCATCGCCATGGCGGACGG
CGAGAAGGTGGTAATCGGCAGCGAAGGCGCTTATCCGCCCTTCAACAATCTCGAATCCGACGGCACGCTGACCGGCTTCG
ACATCGACATCGCCAAGGCGCTTTGCGAAGAGATGAAAGCGGACTGTACTTTCGTCACACAGGAATGGGACGGAATGATT
CCGGCGCTGATCGCCAAGAAATTCGATGCGATCGTCGCGTCGATGTCGATCACGGAAGAGCGCAAGCAACAGGTCGACTT
TACCAACAAATACTACAACACGCCGCCGGCAATCGTCGTGCCGAAGGATTCACCGATTACCGAGGCAACCGCGGCCGGCC
TTTCCGGCAAGGTGCTGGGCGCGCAAAGCTCCACCACCCATTCCAACTATGCGGAAGCGCATATGAAGGAGTCTGAGCTG
AAGCTCTATCCAACCGCGGATGAATACAAGCTCGATCTTGCCAACGGCCGCATCGACGCGGCGATCGACGACGTCGTCGT
GCTTTCGGAATGGCTGAAGACCGAGGACGGCGCCTGCTGCAAGCTGCTCGGCACCCTGCCCATCGATCCCGTCATCAATG
GCGAGGGCGCCGGCATCGCAGTGCGCAAAGGCGATGACGCGCTGCGCGAAAAGCTCAACAAGGCCATCGAGGCCATCCGC
GCGAATGGCAAGTACAAGCAAATCAACGAAAAGTACTTCCCCTTCGACGTCTACGGTAGCTAA

Upstream 100 bases:

>100_bases
CCGTACGAATATCGCAGCAACCGGCGGCGCAAGCCCCGGCGCCAATTGGCATCCGCGAAACAAGAAAAACCGGATGCCGA
AAGCCAACAGAGAGGACACA

Downstream 100 bases:

>100_bases
TCGCAGCGTTCCCGCGGTATTTCGGCTTTGACGGCGGGATAACCTGTTTTGAAGAAGAAAATGCAACGGCGGATGGGCTT
GCCCTCCCGCCGTTTTTGTT

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLISKRLAATASAAVFVLMTGIAMADGEKVVIGSEGAYPPFNNLESDGTLTGFDIDIAKALCEEMKADCTFVTQEWDGMI
PALIAKKFDAIVASMSITEERKQQVDFTNKYYNTPPAIVVPKDSPITEATAAGLSGKVLGAQSSTTHSNYAEAHMKESEL
KLYPTADEYKLDLANGRIDAAIDDVVVLSEWLKTEDGACCKLLGTLPIDPVINGEGAGIAVRKGDDALREKLNKAIEAIR
ANGKYKQINEKYFPFDVYGS

Sequences:

>Translated_260_residues
MLISKRLAATASAAVFVLMTGIAMADGEKVVIGSEGAYPPFNNLESDGTLTGFDIDIAKALCEEMKADCTFVTQEWDGMI
PALIAKKFDAIVASMSITEERKQQVDFTNKYYNTPPAIVVPKDSPITEATAAGLSGKVLGAQSSTTHSNYAEAHMKESEL
KLYPTADEYKLDLANGRIDAAIDDVVVLSEWLKTEDGACCKLLGTLPIDPVINGEGAGIAVRKGDDALREKLNKAIEAIR
ANGKYKQINEKYFPFDVYGS
>Mature_260_residues
MLISKRLAATASAAVFVLMTGIAMADGEKVVIGSEGAYPPFNNLESDGTLTGFDIDIAKALCEEMKADCTFVTQEWDGMI
PALIAKKFDAIVASMSITEERKQQVDFTNKYYNTPPAIVVPKDSPITEATAAGLSGKVLGAQSSTTHSNYAEAHMKESEL
KLYPTADEYKLDLANGRIDAAIDDVVVLSEWLKTEDGACCKLLGTLPIDPVINGEGAGIAVRKGDDALREKLNKAIEAIR
ANGKYKQINEKYFPFDVYGS

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=252, Percent_Identity=39.6825396825397, Blast_Score=186, Evalue=9e-49,
Organism=Escherichia coli, GI1788648, Length=235, Percent_Identity=43.4042553191489, Blast_Score=178, Evalue=2e-46,
Organism=Escherichia coli, GI1787088, Length=238, Percent_Identity=37.3949579831933, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI1788649, Length=235, Percent_Identity=37.4468085106383, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787031, Length=226, Percent_Identity=31.858407079646, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788228, Length=230, Percent_Identity=30, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28026; Mature: 28026

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLISKRLAATASAAVFVLMTGIAMADGEKVVIGSEGAYPPFNNLESDGTLTGFDIDIAKA
CCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCEEECHHHHHHH
LCEEMKADCTFVTQEWDGMIPALIAKKFDAIVASMSITEERKQQVDFTNKYYNTPPAIVV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEE
PKDSPITEATAAGLSGKVLGAQSSTTHSNYAEAHMKESELKLYPTADEYKLDLANGRIDA
ECCCCCCHHHHCCCCCEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCEECH
AIDDVVVLSEWLKTEDGACCKLLGTLPIDPVINGEGAGIAVRKGDDALREKLNKAIEAIR
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHH
ANGKYKQINEKYFPFDVYGS
CCCCEEECCCCCCCEEECCC
>Mature Secondary Structure
MLISKRLAATASAAVFVLMTGIAMADGEKVVIGSEGAYPPFNNLESDGTLTGFDIDIAKA
CCCCHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCCCCCCCCCCCCCCEEECHHHHHHH
LCEEMKADCTFVTQEWDGMIPALIAKKFDAIVASMSITEERKQQVDFTNKYYNTPPAIVV
HHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEE
PKDSPITEATAAGLSGKVLGAQSSTTHSNYAEAHMKESELKLYPTADEYKLDLANGRIDA
ECCCCCCHHHHCCCCCEEECCCCCCCCCHHHHHHCCCCCEEEEECCCCEEEEECCCEECH
AIDDVVVLSEWLKTEDGACCKLLGTLPIDPVINGEGAGIAVRKGDDALREKLNKAIEAIR
HHHHHHHHHHHHCCCCCCCEEEEECCCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHHH
ANGKYKQINEKYFPFDVYGS
CCCCEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]