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The map label for this gene is traI [H]

Identifier: 150396770

GI number: 150396770

Start: 1654170

End: 1654814

Strand: Direct

Name: traI [H]

Synonym: Smed_1560

Alternate gene names: 150396770

Gene position: 1654170-1654814 (Clockwise)

Preceding gene: 150396769

Following gene: 150396771

Centisome position: 43.74

GC content: 60.47

Gene sequence:

>645_bases
ATGATCAGGATAGTAAACGGAAACGGTCGCAGCCAACATCCCCAGGCCATCGACGAGATGTTCCGGCTGCGTAAGCGCGT
GTTCCACGACTTCTTGAAATGGGACGTAAAAACCGAAGGGGATTGGGAAATCGATCACTACGACAAAGCCAATCCGCTCT
ATGTGATGTCGTACTCGCCGGAAACCGGCAAGATCCGGGGCTCCCTCAGGCTCCTTCCGACACTCGGACCGAATATGCTG
GATGACACCTTCCCTATCCTTCTCGGGGACAATCCGGAAATCCGTAGTGCGTCGGTGTGGGAGTCGAGCCGCTTCTGCAT
CGACCCTGAAATCTCCCAGGACCGGGCATCGAATCAGGTCACGGTGGCCGCCGCCGAGTTGATGTGCGGCGTCGGCGAAA
TGGGCCTTGCCTCGGGCATCAGCCATATCGTCACCGTCACCGACGTGTTCCTCGAGCGGATGTTCCGCCGCATGGGCTGC
CCCGGAGAACGCATCGCCGACCCGCACAGGATCGGCTCCGTCTACGCGGTTGCCATTGCCTGGGAGGTCAGCAGGACCCT
GCTCGATACGATGAAGGCGGTAGCGGCCATCGAGGGCACCGTCCTCGAGCAACCGATGTCGCTCGAAACGGCGCGTGCCG
CTTAG

Upstream 100 bases:

>100_bases
TGGGAAAAAATGTAGAAATAAACTGTCACTATAGACAGTTACATGTGTCGTGAGAGCCTGACAGCATCGCTGCATCGCGT
AATAACGCATGGAGCGAAAA

Downstream 100 bases:

>100_bases
CGAATTTCCGGGCCGTGCAGTTCGCACGGCCAACAGCTTCATTCAGGGGTCTTGCGCATAGATCGGGTGAGGAATGTTGT
GTAACGCAAACGTCCTCTCC

Product: autoinducer synthesis protein

Products: NA

Alternate protein names: Autoinducer synthesis protein traI [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MIRIVNGNGRSQHPQAIDEMFRLRKRVFHDFLKWDVKTEGDWEIDHYDKANPLYVMSYSPETGKIRGSLRLLPTLGPNML
DDTFPILLGDNPEIRSASVWESSRFCIDPEISQDRASNQVTVAAAELMCGVGEMGLASGISHIVTVTDVFLERMFRRMGC
PGERIADPHRIGSVYAVAIAWEVSRTLLDTMKAVAAIEGTVLEQPMSLETARAA

Sequences:

>Translated_214_residues
MIRIVNGNGRSQHPQAIDEMFRLRKRVFHDFLKWDVKTEGDWEIDHYDKANPLYVMSYSPETGKIRGSLRLLPTLGPNML
DDTFPILLGDNPEIRSASVWESSRFCIDPEISQDRASNQVTVAAAELMCGVGEMGLASGISHIVTVTDVFLERMFRRMGC
PGERIADPHRIGSVYAVAIAWEVSRTLLDTMKAVAAIEGTVLEQPMSLETARAA
>Mature_214_residues
MIRIVNGNGRSQHPQAIDEMFRLRKRVFHDFLKWDVKTEGDWEIDHYDKANPLYVMSYSPETGKIRGSLRLLPTLGPNML
DDTFPILLGDNPEIRSASVWESSRFCIDPEISQDRASNQVTVAAAELMCGVGEMGLASGISHIVTVTDVFLERMFRRMGC
PGERIADPHRIGSVYAVAIAWEVSRTLLDTMKAVAAIEGTVLEQPMSLETARAA

Specific function: Required for the synthesis of OHHL (N-(3-oxohexanoyl)-L- homoserine lactone), an autoinducer molecule which binds to traR and thus acts in the control of conjugal transfer [H]

COG id: COG3916

COG function: function code TQ; N-acyl-L-homoserine lactone synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the autoinducer synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016181
- InterPro:   IPR001690
- InterPro:   IPR018311 [H]

Pfam domain/function: PF00765 Autoind_synth [H]

EC number: =2.3.1.184 [H]

Molecular weight: Translated: 23880; Mature: 23880

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRIVNGNGRSQHPQAIDEMFRLRKRVFHDFLKWDVKTEGDWEIDHYDKANPLYVMSYSP
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
ETGKIRGSLRLLPTLGPNMLDDTFPILLGDNPEIRSASVWESSRFCIDPEISQDRASNQV
CCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEE
TVAAAELMCGVGEMGLASGISHIVTVTDVFLERMFRRMGCPGERIADPHRIGSVYAVAIA
EEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEHH
WEVSRTLLDTMKAVAAIEGTVLEQPMSLETARAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MIRIVNGNGRSQHPQAIDEMFRLRKRVFHDFLKWDVKTEGDWEIDHYDKANPLYVMSYSP
CEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEECC
ETGKIRGSLRLLPTLGPNMLDDTFPILLGDNPEIRSASVWESSRFCIDPEISQDRASNQV
CCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEEECCCCCCCCCCCEE
TVAAAELMCGVGEMGLASGISHIVTVTDVFLERMFRRMGCPGERIADPHRIGSVYAVAIA
EEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEHH
WEVSRTLLDTMKAVAAIEGTVLEQPMSLETARAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8197112; 9829924; 11743193; 11743194 [H]