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The map label for this gene is dme [H]

Identifier: 150396768

GI number: 150396768

Start: 1650759

End: 1653071

Strand: Direct

Name: dme [H]

Synonym: Smed_1558

Alternate gene names: 150396768

Gene position: 1650759-1653071 (Clockwise)

Preceding gene: 150396765

Following gene: 150396769

Centisome position: 43.65

GC content: 62.26

Gene sequence:

>2313_bases
ATGAACACGGGCGATAAAGCGAAATCCCAAGCGGTTCCCGCCAGCGGAGATATCGATCAGCAGGCACTCTTCTTTCATCG
CTATCCTCGCCCCGGCAAACTCGAGATCCAGCCGACAAAACCGCTCGGGAACCAGCGCGACCTGGCCCTCGCCTATTCTC
CCGGTGTCGCCGCACCGTGCCTCGCCATCAGGGACAACCCTGAAACTGCGGCCGATTTTACCGCTCGCGCCAACCTCGTC
GCGGTGGTGTCCAACGGCACAGCCGTTCTCGGGCTCGGCAATATCGGCCCTCTCGCCTCCAAGCCCGTCATGGAAGGAAA
GGCCGTTCTCTTCAAGAAGTTCGCAGGGATCGACGTCTTCGACATCGAGATCGATGCGCCGACGGTCGAGCGGATGGTCG
ACGTCATCTCCGCTCTGGAGCCGACTTTCGGCGGCATCAATCTCGAGGACATAAAGGCACCCGAATGCTTCGAGGTGGAG
CGACGCCTGCGCGAGAAAATGGAAATACCGGTTTTTCACGATGACCAGCACGGAACCGCGATCATCGTTGCGGCGGCCGT
ACTGAACGGCCTGGAACTCGCCGGCAAGGACATCGCCAAGGCCAAGATCGTGGCTTCGGGTGCCGGAGCTGCGGCGCTGG
CCTGTCTCAACCTGCTCGTCACGCTTGGCGCCAGACGCGAAAACATCTGGGTCCATGATATCGAGGGTCTGGTCTACCAG
GGGCGCGAGGTCCTGATGGACGAATGGAAAGCGGTCTACGCGCAGGAAAGCGACCACCGCGTTCTCGCCGACAGCATCGC
TGGCGCCGACGTGTTTCTGGGCCTGTCGGCCGCAGGCGTTCTCAAGCCCGAGCTGCTGGTTCAAATGGCTGAGAAGCCGC
TGATCATGGCGCTCGCCAATCCGACGCCGGAGATCATGCCCGAGATGGCCCGTGCCGCGCGTCCCGACGCCATGATCTGC
ACCGGACGATCGGACTTTCCCAACCAGGTCAACAATGTCCTCTGTTTCCCGCATATCTTTCGCGGCGCTCTCGATTGCGG
CGCACGTACCATCAACGAAGAGATGAAGATGGCGGCCGTGCGCGCGATCGCCGGTCTTGCCCGCGAAGAACCGTCGGACG
TCGCCGCCCGCGCCTATTCCGGCGAAACGCCGGTTTTCGGCCCCGATTACCTGATCCCCTCGCCCTTCGATCAGCGGCTG
ATCCTGCGCATTGCGCCGGCCGTCGCGAGGGCTGCGGCCGAAAGCGGCGTCGCCACGCGCCCGATCCAGGATTTCGACGC
CTATCTCGACAATCTCAACCGGTTCGTCTTCCGCTCCGGCTTCATCATGAAGCCCGTCTTCGCCGCAGCGAAGAATGCGT
CGAAGAACCGCGTCATCTTCGCCGAGGGCGAAGACGAGCGGGTCTTGCGCGCCGCCCAGGTGCTGCTGGAAGAAGGTACG
GCCAAGCCTATCCTGATCGGCCGCCCGCAAATCATCGAAACCCGACTGCGGCGCTATGGCCTCCGTATCCGCCCCGATAT
CGACTTCGAGGTGGTCAATCCTGAAGGCGACCCACGCTATCGCGACTATGTCGATGATTATTTCGCCCTCGTTGGTCGGC
TCGGCGTCATTCCCGAGGCGGCTCGCACGATCGTACGCACGAACACGACCGTGATCGGGGCGCTTGCAGTCAAACGCGGC
GAGGCCGACGCGCTGATCTGCGGCGTCGAAGGCCGCTACAGCAGGCATCTGCGCGATGTGTCGCAGATCATCGGCAAGCG
TTCGGGCGTGCTCGATTTCTCGGCGCTCAGCCTTCTGATCTCGCAGCGTGGCGCGACCTTCTTCACGGATACCTATGTGA
GCTTCAGTCCGAGTGCGGAGGAGATCGCTCAGACCACGGTGATGGCGGCAAACGAGATCCGCCGCTTCGGAATCACGCCG
CGCGCCGCTCTCGTCTCCCATTCGAATTTCGGCTCACGCGATTCCGAGAGTGCCTTCAAAATGCGCGCTGCTTTGCAGAT
CGTGCGCGAGCTCGCGCCTGATCTCGAGGTGGACGGGGAAATGCATGGCGATTCCGCCATTTCCGAGGGCCTGCGCCAAC
GCGTGATGCCTGACAGTACACTGAACGGCGAGGCCAATCTGCTCGTTTTCCCCAATCTCGACGCCGCCAACATAACGCTT
GGTGTCGTCAAGACCATGACGGACAGTCTGCATGTCGGCCCGATCCTTCTCGGCTCCGCACTTCCCGCGCATATTCTGTC
GCCATCAGTCACGTCCCGCGGCGTCGTCAACATGGCAGCTCTGGCGGTCGTCGAATCAAGCTATCCCGTCTGA

Upstream 100 bases:

>100_bases
ATTCATGCCAAAAGGCACGACATCGATCAGCATCGGACATGAACGCACTCCCGAACCCGCGCTCTCAAGTCCTACGCAAG
AAGTCAAGGAAAACAGAGGC

Downstream 100 bases:

>100_bases
GGGATGGCACTTCCTCCTCGACCATGAGCCGCGGAGAATCGCTGTTTTCCACCGTAGAGATGCATTCGCATCGGCAAAAA
TCATCGATACTGCAATCTGC

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 770; Mature: 770

Protein sequence:

>770_residues
MNTGDKAKSQAVPASGDIDQQALFFHRYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIRDNPETAADFTARANLV
AVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPTVERMVDVISALEPTFGGINLEDIKAPECFEVE
RRLREKMEIPVFHDDQHGTAIIVAAAVLNGLELAGKDIAKAKIVASGAGAAALACLNLLVTLGARRENIWVHDIEGLVYQ
GREVLMDEWKAVYAQESDHRVLADSIAGADVFLGLSAAGVLKPELLVQMAEKPLIMALANPTPEIMPEMARAARPDAMIC
TGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAVRAIAGLAREEPSDVAARAYSGETPVFGPDYLIPSPFDQRL
ILRIAPAVARAAAESGVATRPIQDFDAYLDNLNRFVFRSGFIMKPVFAAAKNASKNRVIFAEGEDERVLRAAQVLLEEGT
AKPILIGRPQIIETRLRRYGLRIRPDIDFEVVNPEGDPRYRDYVDDYFALVGRLGVIPEAARTIVRTNTTVIGALAVKRG
EADALICGVEGRYSRHLRDVSQIIGKRSGVLDFSALSLLISQRGATFFTDTYVSFSPSAEEIAQTTVMAANEIRRFGITP
RAALVSHSNFGSRDSESAFKMRAALQIVRELAPDLEVDGEMHGDSAISEGLRQRVMPDSTLNGEANLLVFPNLDAANITL
GVVKTMTDSLHVGPILLGSALPAHILSPSVTSRGVVNMAALAVVESSYPV

Sequences:

>Translated_770_residues
MNTGDKAKSQAVPASGDIDQQALFFHRYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIRDNPETAADFTARANLV
AVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPTVERMVDVISALEPTFGGINLEDIKAPECFEVE
RRLREKMEIPVFHDDQHGTAIIVAAAVLNGLELAGKDIAKAKIVASGAGAAALACLNLLVTLGARRENIWVHDIEGLVYQ
GREVLMDEWKAVYAQESDHRVLADSIAGADVFLGLSAAGVLKPELLVQMAEKPLIMALANPTPEIMPEMARAARPDAMIC
TGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAVRAIAGLAREEPSDVAARAYSGETPVFGPDYLIPSPFDQRL
ILRIAPAVARAAAESGVATRPIQDFDAYLDNLNRFVFRSGFIMKPVFAAAKNASKNRVIFAEGEDERVLRAAQVLLEEGT
AKPILIGRPQIIETRLRRYGLRIRPDIDFEVVNPEGDPRYRDYVDDYFALVGRLGVIPEAARTIVRTNTTVIGALAVKRG
EADALICGVEGRYSRHLRDVSQIIGKRSGVLDFSALSLLISQRGATFFTDTYVSFSPSAEEIAQTTVMAANEIRRFGITP
RAALVSHSNFGSRDSESAFKMRAALQIVRELAPDLEVDGEMHGDSAISEGLRQRVMPDSTLNGEANLLVFPNLDAANITL
GVVKTMTDSLHVGPILLGSALPAHILSPSVTSRGVVNMAALAVVESSYPV
>Mature_770_residues
MNTGDKAKSQAVPASGDIDQQALFFHRYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPCLAIRDNPETAADFTARANLV
AVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEIDAPTVERMVDVISALEPTFGGINLEDIKAPECFEVE
RRLREKMEIPVFHDDQHGTAIIVAAAVLNGLELAGKDIAKAKIVASGAGAAALACLNLLVTLGARRENIWVHDIEGLVYQ
GREVLMDEWKAVYAQESDHRVLADSIAGADVFLGLSAAGVLKPELLVQMAEKPLIMALANPTPEIMPEMARAARPDAMIC
TGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAVRAIAGLAREEPSDVAARAYSGETPVFGPDYLIPSPFDQRL
ILRIAPAVARAAAESGVATRPIQDFDAYLDNLNRFVFRSGFIMKPVFAAAKNASKNRVIFAEGEDERVLRAAQVLLEEGT
AKPILIGRPQIIETRLRRYGLRIRPDIDFEVVNPEGDPRYRDYVDDYFALVGRLGVIPEAARTIVRTNTTVIGALAVKRG
EADALICGVEGRYSRHLRDVSQIIGKRSGVLDFSALSLLISQRGATFFTDTYVSFSPSAEEIAQTTVMAANEIRRFGITP
RAALVSHSNFGSRDSESAFKMRAALQIVRELAPDLEVDGEMHGDSAISEGLRQRVMPDSTLNGEANLLVFPNLDAANITL
GVVKTMTDSLHVGPILLGSALPAHILSPSVTSRGVVNMAALAVVESSYPV

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505145, Length=350, Percent_Identity=26.5714285714286, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI4505143, Length=396, Percent_Identity=22.979797979798, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI62420882, Length=352, Percent_Identity=25.2840909090909, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI62420880, Length=352, Percent_Identity=25.2840909090909, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI239049447, Length=352, Percent_Identity=25.2840909090909, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI270265879, Length=317, Percent_Identity=27.4447949526814, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788806, Length=752, Percent_Identity=59.1755319148936, Blast_Score=911, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=313, Percent_Identity=32.5878594249201, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1788635, Length=345, Percent_Identity=27.2463768115942, Blast_Score=126, Evalue=5e-30,
Organism=Escherichia coli, GI87081919, Length=294, Percent_Identity=26.1904761904762, Blast_Score=70, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17537199, Length=410, Percent_Identity=24.1463414634146, Blast_Score=73, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=28.4132841328413, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI281362674, Length=421, Percent_Identity=27.0783847980998, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI281362672, Length=421, Percent_Identity=27.0783847980998, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356279, Length=421, Percent_Identity=27.0783847980998, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 83002; Mature: 83002

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTGDKAKSQAVPASGDIDQQALFFHRYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPC
CCCCCCCHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCE
LAIRDNPETAADFTARANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCCHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAPTVERMVDVISALEPTFGGINLEDIKAPECFEVERRLREKMEIPVFHDDQHGTA
EEEECCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IIVAAAVLNGLELAGKDIAKAKIVASGAGAAALACLNLLVTLGARRENIWVHDIEGLVYQ
EEHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
GREVLMDEWKAVYAQESDHRVLADSIAGADVFLGLSAAGVLKPELLVQMAEKPLIMALAN
HHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHEEECCCCCCCCHHHHHHHHCCCEEEEECC
PTPEIMPEMARAARPDAMICTGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAV
CCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHH
RAIAGLAREEPSDVAARAYSGETPVFGPDYLIPSPFDQRLILRIAPAVARAAAESGVATR
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PIQDFDAYLDNLNRFVFRSGFIMKPVFAAAKNASKNRVIFAEGEDERVLRAAQVLLEEGT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
AKPILIGRPQIIETRLRRYGLRIRPDIDFEVVNPEGDPRYRDYVDDYFALVGRLGVIPEA
CCCEEECCCHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
ARTIVRTNTTVIGALAVKRGEADALICGVEGRYSRHLRDVSQIIGKRSGVLDFSALSLLI
HHHHHHCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SQRGATFFTDTYVSFSPSAEEIAQTTVMAANEIRRFGITPRAALVSHSNFGSRDSESAFK
HHCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCHHHHH
MRAALQIVRELAPDLEVDGEMHGDSAISEGLRQRVMPDSTLNGEANLLVFPNLDAANITL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEH
GVVKTMTDSLHVGPILLGSALPAHILSPSVTSRGVVNMAALAVVESSYPV
HHHHHHHCCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNTGDKAKSQAVPASGDIDQQALFFHRYPRPGKLEIQPTKPLGNQRDLALAYSPGVAAPC
CCCCCCCHHCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCE
LAIRDNPETAADFTARANLVAVVSNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCCHHHHHHHCCCEEEEEECCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEIDAPTVERMVDVISALEPTFGGINLEDIKAPECFEVERRLREKMEIPVFHDDQHGTA
EEEECCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCE
IIVAAAVLNGLELAGKDIAKAKIVASGAGAAALACLNLLVTLGARRENIWVHDIEGLVYQ
EEHHHHHHHHHHHCCCCHHHEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCHHHHH
GREVLMDEWKAVYAQESDHRVLADSIAGADVFLGLSAAGVLKPELLVQMAEKPLIMALAN
HHHHHHHHHHHHHCCCCCCEEHHHHHCCCHHEEECCCCCCCCHHHHHHHHCCCEEEEECC
PTPEIMPEMARAARPDAMICTGRSDFPNQVNNVLCFPHIFRGALDCGARTINEEMKMAAV
CCHHHHHHHHHHCCCCCEEEECCCCCCHHHHCEEECHHHHHHHHHHHHHHHHHHHHHHHH
RAIAGLAREEPSDVAARAYSGETPVFGPDYLIPSPFDQRLILRIAPAVARAAAESGVATR
HHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCC
PIQDFDAYLDNLNRFVFRSGFIMKPVFAAAKNASKNRVIFAEGEDERVLRAAQVLLEEGT
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHCCC
AKPILIGRPQIIETRLRRYGLRIRPDIDFEVVNPEGDPRYRDYVDDYFALVGRLGVIPEA
CCCEEECCCHHHHHHHHHCCCEECCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHH
ARTIVRTNTTVIGALAVKRGEADALICGVEGRYSRHLRDVSQIIGKRSGVLDFSALSLLI
HHHHHHCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
SQRGATFFTDTYVSFSPSAEEIAQTTVMAANEIRRFGITPRAALVSHSNFGSRDSESAFK
HHCCCEEEEECEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCHHHHH
MRAALQIVRELAPDLEVDGEMHGDSAISEGLRQRVMPDSTLNGEANLLVFPNLDAANITL
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEEH
GVVKTMTDSLHVGPILLGSALPAHILSPSVTSRGVVNMAALAVVESSYPV
HHHHHHHCCCCCCHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]