Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 150396721

GI number: 150396721

Start: 1589918

End: 1591156

Strand: Direct

Name: bcr [H]

Synonym: Smed_1509

Alternate gene names: 150396721

Gene position: 1589918-1591156 (Clockwise)

Preceding gene: 150396720

Following gene: 150396722

Centisome position: 42.04

GC content: 59.97

Gene sequence:

>1239_bases
ATGACAAGGCCACTAGATCAAACTGCCACCCTCTCCGGATTGACCAAGGTTCAGTTCATTGCTCTCATGGCAATGCTTAT
GTCGATCAACGCGATCTCGATCGACATCATGTTGCCGGGGCTGCAGGAAATCGGCGCGAGCCTCGGTGTGGCCGATGAGA
ACCACCGGCAATACGTCATAACCGCCTACCTGCTCGGCATGGGATTTGCGCAGCTGTTCTTTGGCCCGCTATCCGATCGT
TTCGGGCGAAAGGCACCCTTGTTGGGAGGCCTGGCGCTTTACGGCGTCTGCGCACTCGCGATCGTCTTCGTCCCGACATT
CACGACGCTCCTGTTCCTTCGGCTCGTACAGGGCGTAGGCGCGGCGGCAACCCGCGTCATCACTGTCTCAATAGTGCGCG
ATGTCTATGGCGGGCGGCAAATGGCAGAGATCATGTCGCTGGTCATGATGGTGTTCATGATCGTCCCGGTCATTGCACCG
AGTATCGGGCAATTGATCATGCTGTTTTCCGAATGGCACATGATCTTCGTCGTAATCGGATTGTTTGCGGTCGCCGTCGC
GCTTCCCGTATCGCTGCGGCTTCGTGAAACCCTGGCCCCCGAGAACCGGCGCGCCTTCACGGCGTCATCCGTGATCGCCG
GCTTCCGCATCGTCCTGACGAACCGGCTGGCGCTCTTCTACACCCTGGCAACCTCGGCGCTTCTCGGCGGCCTGTTCGGC
TTTATCAACTCTGCGCAGCAGATTCTCGTCGGAGTCTATGGTCTCGGCGTCTGGTTTCCGGCTGTTTTCGCCGCTTTCGC
CGGCATGATGGCGGTCGCCTCCTTCACCAATTCGCGCCTCGTGCGGACGTTCGGCATGCGGGCATTGTCGCATGCCGCAC
TTCTCGGCTTCACGCTGGCGAGTTTCATCTGGATGTCGGCTTCCCTGATCGGGCCGCTGCCTCTGCCGCTCTTCATGCTT
CTCCATGCCGCCACCATGTTTCAGTTCGGGCTGATAGCCGCAAACTTCAACGCCATGGCGATGGAGCCGCTCGGTCACGT
GGCTGGCACCGCCTCCTCTGTTCTTGGTTTCACGCAGACGATCGGCGGCGGCATCATCGGTGCGTTCATCGGCCAAGCCT
TCGACAATAGCGTCACGCCCCTTGCGATCGGCTTCTTTACAGTGGCCGTCGTCGCCCTGGCCTGTGTGCTGATCGCAGAG
GGCGGAAAGCTATTCAAACCGCACAATCCGGCGGGTTAG

Upstream 100 bases:

>100_bases
GAATATTCAGTGAACTCGATCATTGCGAAACGTGCCACGCACTGGCTCAGCGCGGCAAAGAACCGGCTGCGGGCAAGCTG
CAGCCGGCAGGATGTATGAA

Downstream 100 bases:

>100_bases
CCGGATTCCCCAGTTTTCCCCATCCATTTCGGAATTTCAGACATGTCTTCCGTTTCCCAGCAATCTTCACTCGGTGAGCA
GTCATCCGGGCACAGCAATC

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MTRPLDQTATLSGLTKVQFIALMAMLMSINAISIDIMLPGLQEIGASLGVADENHRQYVITAYLLGMGFAQLFFGPLSDR
FGRKAPLLGGLALYGVCALAIVFVPTFTTLLFLRLVQGVGAAATRVITVSIVRDVYGGRQMAEIMSLVMMVFMIVPVIAP
SIGQLIMLFSEWHMIFVVIGLFAVAVALPVSLRLRETLAPENRRAFTASSVIAGFRIVLTNRLALFYTLATSALLGGLFG
FINSAQQILVGVYGLGVWFPAVFAAFAGMMAVASFTNSRLVRTFGMRALSHAALLGFTLASFIWMSASLIGPLPLPLFML
LHAATMFQFGLIAANFNAMAMEPLGHVAGTASSVLGFTQTIGGGIIGAFIGQAFDNSVTPLAIGFFTVAVVALACVLIAE
GGKLFKPHNPAG

Sequences:

>Translated_412_residues
MTRPLDQTATLSGLTKVQFIALMAMLMSINAISIDIMLPGLQEIGASLGVADENHRQYVITAYLLGMGFAQLFFGPLSDR
FGRKAPLLGGLALYGVCALAIVFVPTFTTLLFLRLVQGVGAAATRVITVSIVRDVYGGRQMAEIMSLVMMVFMIVPVIAP
SIGQLIMLFSEWHMIFVVIGLFAVAVALPVSLRLRETLAPENRRAFTASSVIAGFRIVLTNRLALFYTLATSALLGGLFG
FINSAQQILVGVYGLGVWFPAVFAAFAGMMAVASFTNSRLVRTFGMRALSHAALLGFTLASFIWMSASLIGPLPLPLFML
LHAATMFQFGLIAANFNAMAMEPLGHVAGTASSVLGFTQTIGGGIIGAFIGQAFDNSVTPLAIGFFTVAVVALACVLIAE
GGKLFKPHNPAG
>Mature_411_residues
TRPLDQTATLSGLTKVQFIALMAMLMSINAISIDIMLPGLQEIGASLGVADENHRQYVITAYLLGMGFAQLFFGPLSDRF
GRKAPLLGGLALYGVCALAIVFVPTFTTLLFLRLVQGVGAAATRVITVSIVRDVYGGRQMAEIMSLVMMVFMIVPVIAPS
IGQLIMLFSEWHMIFVVIGLFAVAVALPVSLRLRETLAPENRRAFTASSVIAGFRIVLTNRLALFYTLATSALLGGLFGF
INSAQQILVGVYGLGVWFPAVFAAFAGMMAVASFTNSRLVRTFGMRALSHAALLGFTLASFIWMSASLIGPLPLPLFMLL
HAATMFQFGLIAANFNAMAMEPLGHVAGTASSVLGFTQTIGGGIIGAFIGQAFDNSVTPLAIGFFTVAVVALACVLIAEG
GKLFKPHNPAG

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=395, Percent_Identity=30.126582278481, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1790146, Length=347, Percent_Identity=30.2593659942363, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI48994889, Length=194, Percent_Identity=32.4742268041237, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790794, Length=195, Percent_Identity=28.2051282051282, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI87082312, Length=350, Percent_Identity=27.1428571428571, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787065, Length=243, Percent_Identity=27.5720164609054, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43672; Mature: 43541

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRPLDQTATLSGLTKVQFIALMAMLMSINAISIDIMLPGLQEIGASLGVADENHRQYVI
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCCCCHHHH
TAYLLGMGFAQLFFGPLSDRFGRKAPLLGGLALYGVCALAIVFVPTFTTLLFLRLVQGVG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AAATRVITVSIVRDVYGGRQMAEIMSLVMMVFMIVPVIAPSIGQLIMLFSEWHMIFVVIG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAVAVALPVSLRLRETLAPENRRAFTASSVIAGFRIVLTNRLALFYTLATSALLGGLFG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FINSAQQILVGVYGLGVWFPAVFAAFAGMMAVASFTNSRLVRTFGMRALSHAALLGFTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SFIWMSASLIGPLPLPLFMLLHAATMFQFGLIAANFNAMAMEPLGHVAGTASSVLGFTQT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGGGIIGAFIGQAFDNSVTPLAIGFFTVAVVALACVLIAEGGKLFKPHNPAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC
>Mature Secondary Structure 
TRPLDQTATLSGLTKVQFIALMAMLMSINAISIDIMLPGLQEIGASLGVADENHRQYVI
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHCCCCCCCCCHHHH
TAYLLGMGFAQLFFGPLSDRFGRKAPLLGGLALYGVCALAIVFVPTFTTLLFLRLVQGVG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AAATRVITVSIVRDVYGGRQMAEIMSLVMMVFMIVPVIAPSIGQLIMLFSEWHMIFVVIG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAVAVALPVSLRLRETLAPENRRAFTASSVIAGFRIVLTNRLALFYTLATSALLGGLFG
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FINSAQQILVGVYGLGVWFPAVFAAFAGMMAVASFTNSRLVRTFGMRALSHAALLGFTLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SFIWMSASLIGPLPLPLFMLLHAATMFQFGLIAANFNAMAMEPLGHVAGTASSVLGFTQT
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
IGGGIIGAFIGQAFDNSVTPLAIGFFTVAVVALACVLIAEGGKLFKPHNPAG
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]