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The map label for this gene is recG [H]

Identifier: 150396615

GI number: 150396615

Start: 1479092

End: 1481197

Strand: Direct

Name: recG [H]

Synonym: Smed_1401

Alternate gene names: 150396615

Gene position: 1479092-1481197 (Clockwise)

Preceding gene: 150396612

Following gene: 150396621

Centisome position: 39.11

GC content: 64.48

Gene sequence:

>2106_bases
ATGCGCCCTGCCCTGCTCGACCCGCTGTTTTCGCCTCTCGATACTTTGCCCGGCATCGGACCAAAGACCGGCGAGCTTTA
CGCCCGTCTGCTCGGGCGCGAGACGATCGAAGACTGCCGCGTCGTGGATCTTCTGTTCCATGCGCCCCATTCACTGATCG
ACCGCCGCCGCCAGCCCGGCATCGCCTATGCTCCAGCCGGTTCGATCGTCACCATTACCGGGCGCGTCGACCGGCACCAG
CCCGCCCCCAGCGGCAGGTCGAACGTGCCATATCGCGTCTTTCTTCACGACGATACCGGCGAACTCGCGCTGACCTTCTT
CCGCGTGAAGGGCAACTGGCTCGAAAAGGCCCTGCCGGTCGACGAGACGGTCATCGTCAGCGGCAAGGTCGACTGGTTCA
ACGGGCGGGCCTCCATGGTACACCCGGATTACATGGTCCGCGCGGCCGAATCGCAGAATATGACGCTCGTCGAACCGGTT
TACGGGCTGACGGCGGGCCTGACTTCCAGGCCGCTTCGCAAGTCCATCGAGGCGGCGGTGGCGCGCGTCCCGGACCTGCC
GGAGTGGCTGGACGGGGCCCTACTTCGCCAGCAGGGTTTCCAAAGCGTCAAGGAGAGTCTTCAGCGCCTGCACGAACCAC
GGGATGACGCAGACATCGACGCGCAGGCTCCCGCGCGCCGCAGGCTAGCCTACGACGAATTCCTGGCGGGGCAGCTTTCG
CTCTCGCTCGTTCGCCAGCGACTGCGCAAAGTGTCCGGCACGCCTGTTCATCCAACGGGCCGATTGAGCAGTCCAGTCAT
CGCCGCCCTGCCCTTCTCGCTGACGAGCAGCCAGTCGACCGCCGTCGACGAGATCCTTCAAGACATGTCCGGAACAGACC
GCATGCTGCGATTGCTGCAGGGTGATGTAGGTTCGGGCAAGACGGCCGTCGCCTTGATGGCGATTCTGGCGGCTCTGGAG
GCCGGCGGCCAGGCGGTGCTGATGGCGCCGACGGAAATTCTCGCGCGCCAGCACCACGCCACCCTTTCGAAGTTGGCGGC
ACCGGCGGGTATCACCATCGACGTCCTGACCGGCAGGACGAAGGGCAAGGAGCGTGACGCGGTACTGGAGCGCATCGCTT
CCGGCGAAACGCAGTTGGTGATCGGCACGCACGCACTTTTTCAGGATGCAGTGAGCTATCGCCACCTCGTCCTCGCCGTG
GTCGATGAGCAGCATCGTTTTGGCGTGCACCAGAGATTGCGTCTGACGGCCAAGGGTGTCTCGCCGCACATGCTCGTCAT
GACCGCGACACCTATTCCGAGAACACTTGTGCTTGCGGCCTTCGGCGACATGGACGTTTCGAAGCTGACGGAGAAGCCGG
CCGGGCGCAAGCCGATCCAGACCGTCACGATACCGAACGAACGGACCGACGAGATCGTGGAGAGGCTCGATGCGGCGCTG
AGCCAAGGCAAGAAGGCCTATTGGATCTGCCCTCTCGTCGAGGAGTCCGAGGAAACCGACGCAATGTCTGCCGATGAGCG
CTACCAGAGCCTTGCTCGACGGTTCGGCAAGGATGTCGGCCTGGTGCACGGGCGCATGACGGGGCCTGAAAAGGATGCCG
TCATGCTCGCCTTCAAGACCGGCGACATCCGGTTGCTCGTGGCAACGACCGTCGTCGAGGTCGGGGTCGACGTGCCGGAC
GCCACGATCATGGTGATCGAACACGCCGAACGCTTCGGCCTTGCGCAACTGCATCAGCTTCGGGGGCGCGTTGGTCGCGG
CGACGAAGCCTCGACCTGCATCCTGCTTTACAAAAGCCCGCTCAGCGAAGCCGGGCGGGCTCGGCTCTCGGTCCTGCGCG
AGACGGAAGACGGCTTTCTGATCGCCGAGGAAGATCTCAGGCTGCGTGGCGAAGGCGAGCTCCTCGGAACGCGCCAATCC
GGCACCCCCGGCTTCCTGATCGCCAGTCTCGAGGCGCATGGCGACCTCCTGGAGATGGCGCGAAAGGATGCCGCCTATGT
GATCGATCGGGATCCGGAACTGACATCGGAACGCGGTGCGGCGCTCAGGATGCTTCTGTATCTCTTCCGTCGCGACGAGG
CGATCCGGTTCCTCCGCGCCGGCTGA

Upstream 100 bases:

>100_bases
GTCCGCCGCCTCGGCGATCGAGATCATTGGGCTCTATAAACGCTGGAAAGCCGGTTGTCAGCCTTGCCAAAGGCGGAATT
ATCGTCGCAATTTCATTGCC

Downstream 100 bases:

>100_bases
TCATCCGGCGCGCCCCTCAAACGCTCTCCTGCTGTTTGATCGCAAGCTGCGGCGCGTTGGCCAGAGGGCTGTAATCGGGG
GCGACGAGGCCTCCGGAAAT

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 701

Protein sequence:

>701_residues
MRPALLDPLFSPLDTLPGIGPKTGELYARLLGRETIEDCRVVDLLFHAPHSLIDRRRQPGIAYAPAGSIVTITGRVDRHQ
PAPSGRSNVPYRVFLHDDTGELALTFFRVKGNWLEKALPVDETVIVSGKVDWFNGRASMVHPDYMVRAAESQNMTLVEPV
YGLTAGLTSRPLRKSIEAAVARVPDLPEWLDGALLRQQGFQSVKESLQRLHEPRDDADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVSGTPVHPTGRLSSPVIAALPFSLTSSQSTAVDEILQDMSGTDRMLRLLQGDVGSGKTAVALMAILAALE
AGGQAVLMAPTEILARQHHATLSKLAAPAGITIDVLTGRTKGKERDAVLERIASGETQLVIGTHALFQDAVSYRHLVLAV
VDEQHRFGVHQRLRLTAKGVSPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTVTIPNERTDEIVERLDAAL
SQGKKAYWICPLVEESEETDAMSADERYQSLARRFGKDVGLVHGRMTGPEKDAVMLAFKTGDIRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKSPLSEAGRARLSVLRETEDGFLIAEEDLRLRGEGELLGTRQS
GTPGFLIASLEAHGDLLEMARKDAAYVIDRDPELTSERGAALRMLLYLFRRDEAIRFLRAG

Sequences:

>Translated_701_residues
MRPALLDPLFSPLDTLPGIGPKTGELYARLLGRETIEDCRVVDLLFHAPHSLIDRRRQPGIAYAPAGSIVTITGRVDRHQ
PAPSGRSNVPYRVFLHDDTGELALTFFRVKGNWLEKALPVDETVIVSGKVDWFNGRASMVHPDYMVRAAESQNMTLVEPV
YGLTAGLTSRPLRKSIEAAVARVPDLPEWLDGALLRQQGFQSVKESLQRLHEPRDDADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVSGTPVHPTGRLSSPVIAALPFSLTSSQSTAVDEILQDMSGTDRMLRLLQGDVGSGKTAVALMAILAALE
AGGQAVLMAPTEILARQHHATLSKLAAPAGITIDVLTGRTKGKERDAVLERIASGETQLVIGTHALFQDAVSYRHLVLAV
VDEQHRFGVHQRLRLTAKGVSPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTVTIPNERTDEIVERLDAAL
SQGKKAYWICPLVEESEETDAMSADERYQSLARRFGKDVGLVHGRMTGPEKDAVMLAFKTGDIRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKSPLSEAGRARLSVLRETEDGFLIAEEDLRLRGEGELLGTRQS
GTPGFLIASLEAHGDLLEMARKDAAYVIDRDPELTSERGAALRMLLYLFRRDEAIRFLRAG
>Mature_701_residues
MRPALLDPLFSPLDTLPGIGPKTGELYARLLGRETIEDCRVVDLLFHAPHSLIDRRRQPGIAYAPAGSIVTITGRVDRHQ
PAPSGRSNVPYRVFLHDDTGELALTFFRVKGNWLEKALPVDETVIVSGKVDWFNGRASMVHPDYMVRAAESQNMTLVEPV
YGLTAGLTSRPLRKSIEAAVARVPDLPEWLDGALLRQQGFQSVKESLQRLHEPRDDADIDAQAPARRRLAYDEFLAGQLS
LSLVRQRLRKVSGTPVHPTGRLSSPVIAALPFSLTSSQSTAVDEILQDMSGTDRMLRLLQGDVGSGKTAVALMAILAALE
AGGQAVLMAPTEILARQHHATLSKLAAPAGITIDVLTGRTKGKERDAVLERIASGETQLVIGTHALFQDAVSYRHLVLAV
VDEQHRFGVHQRLRLTAKGVSPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTVTIPNERTDEIVERLDAAL
SQGKKAYWICPLVEESEETDAMSADERYQSLARRFGKDVGLVHGRMTGPEKDAVMLAFKTGDIRLLVATTVVEVGVDVPD
ATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKSPLSEAGRARLSVLRETEDGFLIAEEDLRLRGEGELLGTRQS
GTPGFLIASLEAHGDLLEMARKDAAYVIDRDPELTSERGAALRMLLYLFRRDEAIRFLRAG

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=692, Percent_Identity=41.4739884393064, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1787357, Length=400, Percent_Identity=37.25, Blast_Score=228, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C; PF01336 tRNA_anti [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 76788; Mature: 76788

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPALLDPLFSPLDTLPGIGPKTGELYARLLGRETIEDCRVVDLLFHAPHSLIDRRRQPG
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
IAYAPAGSIVTITGRVDRHQPAPSGRSNVPYRVFLHDDTGELALTFFRVKGNWLEKALPV
EEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCC
DETVIVSGKVDWFNGRASMVHPDYMVRAAESQNMTLVEPVYGLTAGLTSRPLRKSIEAAV
CCEEEEECCEEEECCCCCEECCHHHEEECCCCCCEEECHHHHHHCCCCCHHHHHHHHHHH
ARVPDLPEWLDGALLRQQGFQSVKESLQRLHEPRDDADIDAQAPARRRLAYDEFLAGQLS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLVRQRLRKVSGTPVHPTGRLSSPVIAALPFSLTSSQSTAVDEILQDMSGTDRMLRLLQ
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
GDVGSGKTAVALMAILAALEAGGQAVLMAPTEILARQHHATLSKLAAPAGITIDVLTGRT
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
KGKERDAVLERIASGETQLVIGTHALFQDAVSYRHLVLAVVDEQHRFGVHQRLRLTAKGV
CCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEEECCHHHCCHHHHHEEEECCC
SPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTVTIPNERTDEIVERLDAAL
CCEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
SQGKKAYWICPLVEESEETDAMSADERYQSLARRFGKDVGLVHGRMTGPEKDAVMLAFKT
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEEEC
GDIRLLVATTVVEVGVDVPDATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKSP
CCEEEEEEHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEECCC
LSEAGRARLSVLRETEDGFLIAEEDLRLRGEGELLGTRQSGTPGFLIASLEAHGDLLEMA
CCHHHHHHHHHHHCCCCCEEEECCCCEEECCCCEEEECCCCCCCEEEEEEHHCCHHHHHH
RKDAAYVIDRDPELTSERGAALRMLLYLFRRDEAIRFLRAG
HCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MRPALLDPLFSPLDTLPGIGPKTGELYARLLGRETIEDCRVVDLLFHAPHSLIDRRRQPG
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
IAYAPAGSIVTITGRVDRHQPAPSGRSNVPYRVFLHDDTGELALTFFRVKGNWLEKALPV
EEEECCCCEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCC
DETVIVSGKVDWFNGRASMVHPDYMVRAAESQNMTLVEPVYGLTAGLTSRPLRKSIEAAV
CCEEEEECCEEEECCCCCEECCHHHEEECCCCCCEEECHHHHHHCCCCCHHHHHHHHHHH
ARVPDLPEWLDGALLRQQGFQSVKESLQRLHEPRDDADIDAQAPARRRLAYDEFLAGQLS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
LSLVRQRLRKVSGTPVHPTGRLSSPVIAALPFSLTSSQSTAVDEILQDMSGTDRMLRLLQ
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
GDVGSGKTAVALMAILAALEAGGQAVLMAPTEILARQHHATLSKLAAPAGITIDVLTGRT
CCCCCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
KGKERDAVLERIASGETQLVIGTHALFQDAVSYRHLVLAVVDEQHRFGVHQRLRLTAKGV
CCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHEEEEEECCHHHCCHHHHHEEEECCC
SPHMLVMTATPIPRTLVLAAFGDMDVSKLTEKPAGRKPIQTVTIPNERTDEIVERLDAAL
CCEEEEEECCCCCCEEEEEECCCCCHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHH
SQGKKAYWICPLVEESEETDAMSADERYQSLARRFGKDVGLVHGRMTGPEKDAVMLAFKT
HCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCEEEEEEEC
GDIRLLVATTVVEVGVDVPDATIMVIEHAERFGLAQLHQLRGRVGRGDEASTCILLYKSP
CCEEEEEEHHHHHHCCCCCCCEEEEEECHHHCCHHHHHHHHCCCCCCCCCCEEEEEECCC
LSEAGRARLSVLRETEDGFLIAEEDLRLRGEGELLGTRQSGTPGFLIASLEAHGDLLEMA
CCHHHHHHHHHHHCCCCCEEEECCCCEEECCCCEEEECCCCCCCEEEEEEHHCCHHHHHH
RKDAAYVIDRDPELTSERGAALRMLLYLFRRDEAIRFLRAG
HCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]