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The map label for this gene is queA [H]

Identifier: 150396485

GI number: 150396485

Start: 1360595

End: 1361677

Strand: Direct

Name: queA [H]

Synonym: Smed_1267

Alternate gene names: 150396485

Gene position: 1360595-1361677 (Clockwise)

Preceding gene: 150396484

Following gene: 150396486

Centisome position: 35.98

GC content: 62.7

Gene sequence:

>1083_bases
ATGCGCGTAGACCTGTTTGATTTCGACCTGCCGGAGGATTCCGTAGCGCTGAGGCCCGCAAGCCCGCGCGATAGCGCCCG
CATGCTCGTCGTTCGTCCCGGTGGCGAACCGGTTCTCGAGGATCGTGGCGTCCTCGATCTGCCGTCGTTCCTGCGGCAAG
GCGATGCGCTGGTCTTCAATGACACCAAGGTCATACCGGCGCAGCTCGAAGGCGTTCGCTACCGGGGTGAGCATATCAGC
ACGCCCGTGTCGCTGACGTTGCATATGCGTGTTGCGCCTGATCGCTGGAAAGCTTTCGCGCGTCCGGCCCGCCGGCTGAA
ACCCGGAGACCGGGTCAGCTTCGGGCATGGCGGCAATGCCTGCCTGCTCGGCTCGCTGGACGCCATGGTCGAAGAGAAAG
GCGATGCCGGCGAGGTGACGCTTCGCTTCGATCTCTCGGGTCCTTCGCTTGATGAAGCGATCATGTCCGTCGGGCACATC
CCTCTGCCGCCTTACATTGCGTCCAAGCGTGCCGACGATGCGCGGGACCGGACCGATTACCAGACTGTCTATGCGCGCGA
GGAAGGCGCGGTGGCGGCGCCGACTGCGGGGCTGCATTTCACCGATCGCCTGTTCGCCATGCTCGACGAGGCAGGCATTG
AGCGGCATTTCGTCACGCTACACGTCGGGGCCGGTACATTCCTGCCGGTCAAGGCGGACGATACCGACGATCATGTGATG
CATGAGGAAATCGGCCATGTCGATGCAGTAACCGCGGCGAAGCTCAATGCAGTGCGGGAACGCGGGGGCCGTGTCGTCTG
TGTCGGCACGACATCGCTTCGGCTGGTCGAGAGCGCAGCCGCCGAAGACGGCACTATACATGCCTGGTCGGGCGCAACCG
GCATTTTCATCACCCCGGGCTACCGCTTCCGCGCGGCCCACATGCTGATGACCAATTTTCACCTGCCGAAATCGACCCTG
TTCATGCTGGTCTCGGCCTTCGCGGGGCTCGAAACAATGCGCGACGCTTATGCCCATGCGATCGCAACGGGGTACCGTTT
CTATTCCTATGGCGATTCGAGCCTGCTCTTCCGGAAAGACTAA

Upstream 100 bases:

>100_bases
TGCGGGTTGCCGCCGACGCCTGATTTGCGCTAATGCACTGACACGCCCGCCCCAGTCGCGCGAGCCGCCGGGGCGGGTTT
GCTTTTGGCTGATATTCTAG

Downstream 100 bases:

>100_bases
ATGACCGAAGCGTTTCAATTCAAACTGCTTTCAAGTGACGGCAATGCACGCCGCGGCGAGGTCTTGACGCCTCGCGGGAC
GATCCGCACGCCAGCCTTCA

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MRVDLFDFDLPEDSVALRPASPRDSARMLVVRPGGEPVLEDRGVLDLPSFLRQGDALVFNDTKVIPAQLEGVRYRGEHIS
TPVSLTLHMRVAPDRWKAFARPARRLKPGDRVSFGHGGNACLLGSLDAMVEEKGDAGEVTLRFDLSGPSLDEAIMSVGHI
PLPPYIASKRADDARDRTDYQTVYAREEGAVAAPTAGLHFTDRLFAMLDEAGIERHFVTLHVGAGTFLPVKADDTDDHVM
HEEIGHVDAVTAAKLNAVRERGGRVVCVGTTSLRLVESAAAEDGTIHAWSGATGIFITPGYRFRAAHMLMTNFHLPKSTL
FMLVSAFAGLETMRDAYAHAIATGYRFYSYGDSSLLFRKD

Sequences:

>Translated_360_residues
MRVDLFDFDLPEDSVALRPASPRDSARMLVVRPGGEPVLEDRGVLDLPSFLRQGDALVFNDTKVIPAQLEGVRYRGEHIS
TPVSLTLHMRVAPDRWKAFARPARRLKPGDRVSFGHGGNACLLGSLDAMVEEKGDAGEVTLRFDLSGPSLDEAIMSVGHI
PLPPYIASKRADDARDRTDYQTVYAREEGAVAAPTAGLHFTDRLFAMLDEAGIERHFVTLHVGAGTFLPVKADDTDDHVM
HEEIGHVDAVTAAKLNAVRERGGRVVCVGTTSLRLVESAAAEDGTIHAWSGATGIFITPGYRFRAAHMLMTNFHLPKSTL
FMLVSAFAGLETMRDAYAHAIATGYRFYSYGDSSLLFRKD
>Mature_360_residues
MRVDLFDFDLPEDSVALRPASPRDSARMLVVRPGGEPVLEDRGVLDLPSFLRQGDALVFNDTKVIPAQLEGVRYRGEHIS
TPVSLTLHMRVAPDRWKAFARPARRLKPGDRVSFGHGGNACLLGSLDAMVEEKGDAGEVTLRFDLSGPSLDEAIMSVGHI
PLPPYIASKRADDARDRTDYQTVYAREEGAVAAPTAGLHFTDRLFAMLDEAGIERHFVTLHVGAGTFLPVKADDTDDHVM
HEEIGHVDAVTAAKLNAVRERGGRVVCVGTTSLRLVESAAAEDGTIHAWSGATGIFITPGYRFRAAHMLMTNFHLPKSTL
FMLVSAFAGLETMRDAYAHAIATGYRFYSYGDSSLLFRKD

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=360, Percent_Identity=43.0555555555556, Blast_Score=246, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39364; Mature: 39364

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDLFDFDLPEDSVALRPASPRDSARMLVVRPGGEPVLEDRGVLDLPSFLRQGDALVFN
CEEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEEE
DTKVIPAQLEGVRYRGEHISTPVSLTLHMRVAPDRWKAFARPARRLKPGDRVSFGHGGNA
CCEEECHHHCCEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCE
CLLGSLDAMVEEKGDAGEVTLRFDLSGPSLDEAIMSVGHIPLPPYIASKRADDARDRTDY
EEEECHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
QTVYAREEGAVAAPTAGLHFTDRLFAMLDEAGIERHFVTLHVGAGTFLPVKADDTDDHVM
EEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCHHH
HEEIGHVDAVTAAKLNAVRERGGRVVCVGTTSLRLVESAAAEDGTIHAWSGATGIFITPG
HHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCC
YRFRAAHMLMTNFHLPKSTLFMLVSAFAGLETMRDAYAHAIATGYRFYSYGDSSLLFRKD
CHHHHHEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEECC
>Mature Secondary Structure
MRVDLFDFDLPEDSVALRPASPRDSARMLVVRPGGEPVLEDRGVLDLPSFLRQGDALVFN
CEEEEEECCCCCCCEEECCCCCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCEEEEE
DTKVIPAQLEGVRYRGEHISTPVSLTLHMRVAPDRWKAFARPARRLKPGDRVSFGHGGNA
CCEEECHHHCCEEECCCCCCCCEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCE
CLLGSLDAMVEEKGDAGEVTLRFDLSGPSLDEAIMSVGHIPLPPYIASKRADDARDRTDY
EEEECHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCC
QTVYAREEGAVAAPTAGLHFTDRLFAMLDEAGIERHFVTLHVGAGTFLPVKADDTDDHVM
EEEEEECCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCHHH
HEEIGHVDAVTAAKLNAVRERGGRVVCVGTTSLRLVESAAAEDGTIHAWSGATGIFITPG
HHHHCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEEECCC
YRFRAAHMLMTNFHLPKSTLFMLVSAFAGLETMRDAYAHAIATGYRFYSYGDSSLLFRKD
CHHHHHEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]