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The map label for this gene is pcm [H]

Identifier: 150396367

GI number: 150396367

Start: 1219126

End: 1219785

Strand: Direct

Name: pcm [H]

Synonym: Smed_1148

Alternate gene names: 150396367

Gene position: 1219126-1219785 (Clockwise)

Preceding gene: 150396366

Following gene: 150396368

Centisome position: 32.24

GC content: 58.94

Gene sequence:

>660_bases
ATGAACTTCGAAGCAGCCCGCATCAAGATGGTGGACAACCAGATCCGGACCACGGATGTGACGTCGCATTCAGTCCTGTC
CGCCTTCCTGTCGGTGCCGCGCGAGGAGTTCGTTCCAGCCAAGATGCGGGAACTGGCCTATATCGACACCGACGTCGAAC
TCGTGGGCGGGGTGCAGCCGCGTTACCTGATGGAGCCATCGCCGCTGGCGAAGCTGCTTCAGCTCGCAGAAATTTCGAAA
TCCGACCTGGTCCTCGAGATTGGCTGCGGCACTGGCTACGCTTCGGCATTGCTTTCGCTTCTCGCCGGTTCCGTCGTGGC
CCTGGAGAGCGACGAAGCCCTCGCTGCGACGGCCACGGCAACGCTGTCGCGTCTCGGTTATGACAACGTCGCCGTCGTGA
GTGGCGATCTGACCAAAGGCTATGCGGCGGAAGCACCCTATGATGTGATCTTCGTCAACGGTGCAGTGGAAATCATACCG
GCCGAACTGTTCGAGCAGCTTCGCGACGGTGGACGCCTCGTGACGGTGGAAGGTTTTGGTAACGCTTCCCGTGCAAAACT
TTATGCGCGCGAATCGGGCATGACATCGGAGCGAGCTGACTTCAATACGGCCGTGAAACCGCTTCCCGGTTTCCTTCTCG
AGCGTAGTTTTGTTTTTTGA

Upstream 100 bases:

>100_bases
GATTGATGCGACACGCTTCAGGCAACAGAGTGGCAGCAGCCCGACGCCAGATCGAACGCTATTCCAAGGCGCCGCGCCCA
TTCCGACACGAGGCAATATC

Downstream 100 bases:

>100_bases
TGGGCCTCTTATTGAGGTAGCTCAACATATGAGTGTTGACGCTGAAACTGCAATGTTGCCGAACGGCAACGTATTATTTG
TTTCTTTTTGAAATGGCCGT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MNFEAARIKMVDNQIRTTDVTSHSVLSAFLSVPREEFVPAKMRELAYIDTDVELVGGVQPRYLMEPSPLAKLLQLAEISK
SDLVLEIGCGTGYASALLSLLAGSVVALESDEALAATATATLSRLGYDNVAVVSGDLTKGYAAEAPYDVIFVNGAVEIIP
AELFEQLRDGGRLVTVEGFGNASRAKLYARESGMTSERADFNTAVKPLPGFLLERSFVF

Sequences:

>Translated_219_residues
MNFEAARIKMVDNQIRTTDVTSHSVLSAFLSVPREEFVPAKMRELAYIDTDVELVGGVQPRYLMEPSPLAKLLQLAEISK
SDLVLEIGCGTGYASALLSLLAGSVVALESDEALAATATATLSRLGYDNVAVVSGDLTKGYAAEAPYDVIFVNGAVEIIP
AELFEQLRDGGRLVTVEGFGNASRAKLYARESGMTSERADFNTAVKPLPGFLLERSFVF
>Mature_219_residues
MNFEAARIKMVDNQIRTTDVTSHSVLSAFLSVPREEFVPAKMRELAYIDTDVELVGGVQPRYLMEPSPLAKLLQLAEISK
SDLVLEIGCGTGYASALLSLLAGSVVALESDEALAATATATLSRLGYDNVAVVSGDLTKGYAAEAPYDVIFVNGAVEIIP
AELFEQLRDGGRLVTVEGFGNASRAKLYARESGMTSERADFNTAVKPLPGFLLERSFVF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=168, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23529; Mature: 23529

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEAARIKMVDNQIRTTDVTSHSVLSAFLSVPREEFVPAKMRELAYIDTDVELVGGVQP
CCCCHHEEEEECCCEEECCCHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCCHHHHCCCCC
RYLMEPSPLAKLLQLAEISKSDLVLEIGCGTGYASALLSLLAGSVVALESDEALAATATA
CEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHHH
TLSRLGYDNVAVVSGDLTKGYAAEAPYDVIFVNGAVEIIPAELFEQLRDGGRLVTVEGFG
HHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCC
NASRAKLYARESGMTSERADFNTAVKPLPGFLLERSFVF
CCHHHEEEECCCCCCCCCCCCCCHHCCCCHHHHHHCCCC
>Mature Secondary Structure
MNFEAARIKMVDNQIRTTDVTSHSVLSAFLSVPREEFVPAKMRELAYIDTDVELVGGVQP
CCCCHHEEEEECCCEEECCCHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCCHHHHCCCCC
RYLMEPSPLAKLLQLAEISKSDLVLEIGCGTGYASALLSLLAGSVVALESDEALAATATA
CEEECCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEECCCCHHHHHHH
TLSRLGYDNVAVVSGDLTKGYAAEAPYDVIFVNGAVEIIPAELFEQLRDGGRLVTVEGFG
HHHHCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHCCCEEEEEECCC
NASRAKLYARESGMTSERADFNTAVKPLPGFLLERSFVF
CCHHHEEEECCCCCCCCCCCCCCHHCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA